Query: NC_013174:23421 Jonesia denitrificans DSM 20603, complete genome
Lineage: Jonesia denitrificans; Jonesia; Jonesiaceae; Actinomycetales; Actinobacteria; Bacteria
General Information: Isolation: Boiled ox blood; Temp: Mesophile; Temp: 37C. This species was formerly classified as Listeria denitrificans, and is the only species of the genus Jonesia. It is a Gram-positive nonspore-forming, motile rod that is catalase positive, oxidase negative, and facultatively anaerobic.
Subject: NC_006177:835459 Symbiobacterium thermophilum IAM 14863, complete genome
Lineage: Symbiobacterium thermophilum; Symbiobacterium; Shewanellaceae; Clostridiales; Firmicutes; Bacteria
General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.