Pre_GI: SWBIT SVG BLASTP

Query: NC_012560:603447 Azotobacter vinelandii DJ, complete genome

Lineage: Azotobacter vinelandii; Azotobacter; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.

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BLASTP Alignment.txt

Subject: NC_010407:316320 Clavibacter michiganensis subsp. sepedonicus chromosome, complete

Lineage: Clavibacter michiganensis; Clavibacter; Microbacteriaceae; Actinomycetales; Actinobacteria; Bacteria

General Information: Causative agent of bacterial ring rot. Isolated from infected potato. This organism was first described and classified in 1914 as "Bacterium sepedonicus" and is considered a major plant pathogen. It is a phytopathogenic actinomycete that causes wilt and tuber rot in potato, which is a plant vascular disease with very high bacterial titers. Pathogenicity is believed to be associated with the presence of two plasmids, pCSL1 and pCSL2. This species is subdivided into five subspecies: michiganensis, sepedonicus, nebraskensis, tesselarius and insidiosus each of which infects specific hosts: tomato, potato, corn, wheat and alfalfa, respectively. Members of the Clavibacter genus are known to produce antimicrobial compounds.