Pre_GI: SWBIT SVG BLASTN

Query: NC_010322:1520973 Pseudomonas putida GB-1 chromosome, complete genome

Lineage: Pseudomonas putida; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: Pseudomonas putida strain GB-1, a fresh water, Gram-negative gamma-proteobacterium, is a genetically tractable, robust manganese (Mn) oxidizer, and as such, is an ideal model for unraveling the catalytic mechanism for, and the molecular regulation of Mn(IV) oxide production and its eventual accumulation on the cell surface at the onset of stationary phase. Since its isolation from Green Bay nearly 20 years ago by Ken Nealson’s group (then at the Center for Great Lakes Studies, Univ. Wisconsin-Milwaukee, USA), it has been the non spore-forming, model organism (along with the closely-related strain MnB1) for molecular genetic studies of Mn(II) oxidization, protein transport and biofilm formation and for biochemical studies on protein purification and Mn(III)-pyoverdine binding. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. As they are metabolically versatile, and well characterized, it makes them great candidates for biocatalysis, bioremediation and other agricultural applications. Certain strains have been used in the production of bioplastics.

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BLASTN Alignment.txt

Subject: NC_003305:1743939 Agrobacterium tumefaciens str. C58 chromosome linear, complete

Lineage: Agrobacterium tumefaciens; Agrobacterium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Gram-negative soil bacterium. This is the most widely studied species in the genus. Strains of Agrobacterium are classified in three biovars based on their utilisation of different carbohydrates and other biochemical tests. The differences between biovars are determined by genes on the single circle of chromosomal DNA. Biovar differences are not particularly relevant to the pathogenicity of A. tumefaciens, except in one respect: biovar 3 is found worldwide as the pathogen of gravevines. This species causes crown gall disease of a wide range of dicotyledonous (broad-leaved) plants, especially members of the rose family such as apple, pear, peach, cherry, almond, raspberry and roses. Because of the way that it infects other organisms, this bacterium has been used as a tool in plant breeding. Any desired genes, such as insecticidal toxin genes or herbicide-resistance genes, can be engineered into the bacterial DNA, and then inserted into the plant genome. This process shortens the conventional plant breeding process, and allows entirely new (non-plant) genes to be engineered into crops.