Pre_GI: SWBIT SVG BLASTP

Query: NC_020134:2832857 Clostridium stercorarium subsp. stercorarium DSM 8532, complete

Lineage: Clostridium stercorarium; Clostridium; unclassified Ruminococcaceae; Clostridiales; Firmicutes; Bacteria

General Information: Lignocellulosic biomass has great potential as an abundant and renewable source of fermentable sugars through enzymic saccharification. Clostridium stercorarium is a catabolically versatile bacterium producing a wide range of hydrolases for degradation of biomass. Together with Clostridium thermocellum, Clostridium aldrichii and other cellulose degraders, it forms group I of the clostridia. It is moderately thermophilic, with an optimum growth temperature of 65 degrees C, and has repeatedly been isolated from self-heated compost. The two-component cellulase system of C. stercorarium has been investigated thoroughly. Due to its ability to utilize the various polysaccharides present in biomass it is especially suited for the fermentation of hemicellulose to organic solvents. Some isolates have been used in Japan in a single-step ethanol-fermenting pilot-process with lignocellulosic biomass as substrate.

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BLASTP Alignment.txt

Subject: NC_007413:1570000 Anabaena variabilis ATCC 29413, complete genome

Lineage: Anabaena variabilis; Anabaena; Nostocaceae; Nostocales; Cyanobacteria; Bacteria

General Information: These cyanobacteria are bluegreen algae that are capable of fixing carbon and nitrogen. They form long filaments and can be found worldwide in various aquatic environments as well as some terrestrial ones. These bacteria can form a variety of differentiated cell types, including spore-like cells (akinetes), small motile filaments (hormongia) and most importantly, heterocysts that are nitrogen-producing cells. The heterocyst produces multiple layers outside of its cell wall, shuts down photosystem II in order to inhibit oxygenic photosynthesis and ramps up metabolism in order to use up the oxygen present. Heterocysts donate fixed nitrogen compounds as amino acids to neighboring cells and in return receive a photosynthetically produced carbon source such as sucrose. These organisms produce toxic blooms in aquatic environments that are harmful or fatal to animals and humans due to the various cyanotoxins they produce. Anabaena variabilis is a filamentous heterocyst-forming cyanobacterium that fixes nitrogen and CO2 using the energy of sunlight via oxygen-evolving plant-type photosynthesis. In addition, this organism has been studied extensively for the production of hydrogen using solar energy.