Query: NC_015379:1887275 Pseudomonas brassicacearum subsp. brassicacearum NFM421 chromosome, Lineage: Pseudomonas brassicacearum; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria General Information: Pseudomonas brassicacearum is a Gram-negative soil bacterium that infects the roots of Brassica napus, from which it derives its name. Based on 16S rRNA analysis, P. brassicacearum falls within the P. fluorescens group. It has also been shown to have both pathogenic and plant growth-promoting effects on tomato plants.
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General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.