Query: NC_013235:1761539 Nakamurella multipartita DSM 44233, complete genome
Lineage: Nakamurella multipartita; Nakamurella; Nakamurellaceae; Actinomycetales; Actinobacteria; Bacteria
General Information: Isolation: Actived sludge cultured in fed-batch; Temp: Mesophile; Temp: 28C; Habitat: Sludge. Nakamurella multipartite DSM 44233 was isolated from activated sludge and is able in store polysaccharides inside the cell. Obligately aerobic chemoorganotrophic, polysaccharide-accumulating Gram-positive bacterium.
Subject: NC_006177:280234 Symbiobacterium thermophilum IAM 14863, complete genome
Lineage: Symbiobacterium thermophilum; Symbiobacterium; Shewanellaceae; Clostridiales; Firmicutes; Bacteria
General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.