Query: NC_012988:3288253 Methylobacterium extorquens DM4, complete genome Lineage: Methylobacterium extorquens; Methylobacterium; Methylobacteriaceae; Rhizobiales; Proteobacteria; Bacteria General Information: Methylobacterium extorquens DM4 was isolated from soil contaminated with halogenated (chlorine-containing) hydrocarbons. This organism is able to use dichloromethane as a sole carbon and energy source. Dichloromethane, an industrial solvent, is toxic and carcinogenic. This compound is water soluble and can easily contaminate groundwater. Methylobacterium chloromethanicum was isolated from soil at a petrochemical factory in Tatarstan, Russia. This organism is able to grow using chloromethane as the sole carbon and energy source.
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General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.