Query: NC_011894:3797500 Methylobacterium nodulans ORS 2060, complete genome
Lineage: Methylobacterium nodulans; Methylobacterium; Methylobacteriaceae; Rhizobiales; Proteobacteria; Bacteria
General Information: Methylobacterium nodulans was isolated from root nodules from the legume Crotalaria, a large Fabaceae group found in Africa. Methylobacterium nodulans fixes nitrogen and can grow with C1 compounds, such as methanol and formate, as a sole carbon source.
Subject: NC_007426:205390 Natronomonas pharaonis DSM 2160, complete genome
Lineage: Natronomonas pharaonis; Natronomonas; Halobacteriaceae; Halobacteriales; Euryarchaeota; Archaea
General Information: Isolated from Lake Gabara in Egypt. Extreme haloalkaliphilic archeon. Natronomonas pharaonis is able to survive at high salt and pH conditions which results in limited nitrogen availability through ammonium. In order to compensate for this, Natronomonas pharaonis has developed three systems to promote nitrogen assimilation: direct uptake of ammonia, uptake of nitrate, and uptake of urea. Another problem with high pH environments is the use of a proton gradient for the generation of ATP, which other alkaliphiles have adapted to by substitution of sodium ions for protons. However, this organism utilizes protons for ATP generation as determined by experimental data.