Pre_GI: SWBIT SVG BLASTP

Query: NC_010556:462500 Exiguobacterium sibiricum 255-15, complete genome

Lineage: Exiguobacterium sibiricum; Exiguobacterium; Bacillales Family XII; Bacillales; Firmicutes; Bacteria

General Information: This organism was isolated from a 2-3 million-year permafrost core in Siberia, Russia and can survive and grow rapidly at low temperatures. Analysis of long-term survival of psychrophilic organisms such as this one may aid understanding of the potential growth of organisms in astrobiology. Exiguobacterium sibiricum is a psychrotolerant organism able to grow at temperatures that range from -6 to 40 degrees C. This organism is also able to survive repeated freeze/thaw cycles which may contribute to its ability to survive in cold environments.

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BLASTP Alignment.txt

Subject: NC_013890:808791 Dehalococcoides sp. GT chromosome, complete genome

Lineage: Dehalococcoides mccartyi; Dehalococcoides; Dehalococcoidaceae; Dehalococcoidales; Chloroflexi; Bacteria

General Information: Temp: Mesophile; Habitat: Fresh water, Groundwater. Dehalococcoides sp. GT was isolated from an chloroethene-contaminated aquifer. This strain can dechlorinate trichloroethene and vinyl chloride. This organism was isolated from environments contaminated with organic chlorinated chemicals such as tetrachloroethene (PCE) and trichloroethane (TCE), common contaminants in the anaerobic subsurface. There are at least 15 organisms from different metabolic groups, halorespirators, acetogens, methanogens and facultative anaerobes, that are able to metabolize PCE. Some of these organisms couple dehalogenation to energy conservation and utilize PCE as the only source of energy while others dehalogenate tetrachloroethene fortuitously. This non-methanogenic, non-acetogenic culture is able to grow with hydrogen as the electron donor, indicating that hydrogen/PCE serves as an electron donor/acceptor for energy conservation and growth. This organism can only grow anaerobically in the presence of hydrogen as an electron donor and chlorinated compounds as electron acceptors. Dehalococcoides ethenogenes is typically found at sites contaminated with chlorinated solvents, and have been independently isolated in dozens of sites across the USA.