Query: NC_010322:1336231 Pseudomonas putida GB-1 chromosome, complete genome Lineage: Pseudomonas putida; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria General Information: Pseudomonas putida strain GB-1, a fresh water, Gram-negative gamma-proteobacterium, is a genetically tractable, robust manganese (Mn) oxidizer, and as such, is an ideal model for unraveling the catalytic mechanism for, and the molecular regulation of Mn(IV) oxide production and its eventual accumulation on the cell surface at the onset of stationary phase. Since its isolation from Green Bay nearly 20 years ago by Ken Nealson’s group (then at the Center for Great Lakes Studies, Univ. Wisconsin-Milwaukee, USA), it has been the non spore-forming, model organism (along with the closely-related strain MnB1) for molecular genetic studies of Mn(II) oxidization, protein transport and biofilm formation and for biochemical studies on protein purification and Mn(III)-pyoverdine binding. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. As they are metabolically versatile, and well characterized, it makes them great candidates for biocatalysis, bioremediation and other agricultural applications. Certain strains have been used in the production of bioplastics.
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General Information: The SL254 strain is an MDR strain from one of two distinct lineages of the Newport serovar. Salmonella enterica subsp. enterica serovar Newport is common worldwide. Outbreak investigations and targeted studies have identified dairy cattle as the main reservoir this serotype. Antimicrobial resistance (Newport MDR-AmpC) is particularly problematic in this serotype, and the prevalence of Newport MDR-AmpC isolates from humans in the United States has increased from 0% during 1996-1997 to 26% in 2001. MDR strains have been recorded as resistant to ampicillin, chloramphenicol, streptomycin, sulphonamides and tetracycline (ACSSuT) and many of these strains show intermediate or full resistance to third-generation cephalosporins, kanamycin, potentiated sulphonamides, and gentamicin. This group of Enterobactericiae have pathogenic characteristics and are one of the most common causes of enteric infections (food poisoning) worldwide. They were named after the scientist Dr. Daniel Salmon who isolated the first organism, Salmonella choleraesuis, from the intestine of a pig. The presence of several pathogenicity islands (PAIs) that encode various virulence factors allows Salmonella spp. to colonize and infect host organisms. There are two important PAIs, Salmonella pathogenicity island 1 and 2 (SPI-1 and SPI-2) that encode two different type III secretion systems for the delivery of effector molecules into the host cell that result in internalization of the bacteria which then leads to systemic spread.