Pre_GI: SWBIT SVG BLASTP

Query: NC_010322:1520973 Pseudomonas putida GB-1 chromosome, complete genome

Lineage: Pseudomonas putida; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: Pseudomonas putida strain GB-1, a fresh water, Gram-negative gamma-proteobacterium, is a genetically tractable, robust manganese (Mn) oxidizer, and as such, is an ideal model for unraveling the catalytic mechanism for, and the molecular regulation of Mn(IV) oxide production and its eventual accumulation on the cell surface at the onset of stationary phase. Since its isolation from Green Bay nearly 20 years ago by Ken Nealson’s group (then at the Center for Great Lakes Studies, Univ. Wisconsin-Milwaukee, USA), it has been the non spore-forming, model organism (along with the closely-related strain MnB1) for molecular genetic studies of Mn(II) oxidization, protein transport and biofilm formation and for biochemical studies on protein purification and Mn(III)-pyoverdine binding. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. As they are metabolically versatile, and well characterized, it makes them great candidates for biocatalysis, bioremediation and other agricultural applications. Certain strains have been used in the production of bioplastics.

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BLASTP Alignment.txt

Subject: NC_007347:767455 Ralstonia eutropha JMP134 chromosome 1, complete sequence

Lineage: Cupriavidus pinatubonensis; Cupriavidus; Burkholderiaceae; Burkholderiales; Proteobacteria; Bacteria

General Information: This organism is found in both soil and water and has great potential for use in bioremediation as it is capable of degrading a large list of pollutants including chlorinated aromatic compounds. The bacterium can utilize hydrogen, carbon dioxide, as well as organic compounds for growth and is a model organism for hydrogen oxidation as it can grow on hydrogen as the sole energy source. It was originally isolated due to its ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid, which is due to the degradative functions being encoded on a plasmid (pJP4). Metabolically versatile bacterium. Cupriavidus necator also known as Ralstonia eutropha is a soil bacterium with diverse metabolic abilities. Strains of this organism are resistant to high levels of copper or are able to degrade chloroaromatic compounds such as halobenzoates and nitrophenols making them useful for bioremediation.