Query: NC_009937:673820 Azorhizobium caulinodans ORS 571, complete genome Lineage: Azorhizobium caulinodans; Azorhizobium; Xanthobacteraceae; Rhizobiales; Proteobacteria; Bacteria General Information: This species is a stem-nodulating nitrogen-fixing organism isolated from Sesbania rostrata. Azorhizobium caulinodans ORS571 is a microsymbiont of the water-tolerant tropical legume Sesbania rostrata.A. caulinodans ORS571 is able to fix nitrogen in the free-living state, which is not the case for most rhizobia.
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General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.