Query: NC_009439:4913767 Pseudomonas mendocina ymp, complete genome Lineage: Pseudomonas mendocina; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria General Information: Soil and subsurface bacterium. Strain ymp was isolated as part of the Yucca Mountain Project from sediment in a surface holding pond of a drilling operation at the Nevada Test Site, NV, USA (the proposed site of the high-level nuclear repository). Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. Pseudomonas mendocina is a pentachlorophenol (PCP)-degrading bacterium was isolated from PCP-contaminated soil. It is able to act as a bioremediation agent without the accumulation of inhibitory toxic compounds.
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General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.