Query: NC_008705:5144433 Mycobacterium sp. KMS, complete genome
Lineage: Mycobacterium; Mycobacterium; Mycobacteriaceae; Actinomycetales; Actinobacteria; Bacteria
General Information: This organism was isolated from creosote-contaminated soil from the Champion International Superfund site in Libby, Montana, USA. This microbe, along with some others collected at this site, are able to rapidly mineralize 14C-labeled pyrene. Phylogenetic studies based on the rRNA 16S gene confirmed that strains collected at this site were distinct from one another and also from other known Mycobacterium isolates.
Subject: NC_006177:2943259 Symbiobacterium thermophilum IAM 14863, complete genome
Lineage: Symbiobacterium thermophilum; Symbiobacterium; Shewanellaceae; Clostridiales; Firmicutes; Bacteria
General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.