Query: NC_007681:1157402 Methanosphaera stadtmanae DSM 3091, complete genome
Lineage: Methanosphaera stadtmanae; Methanosphaera; Methanobacteriaceae; Methanobacteriales; Euryarchaeota; Archaea
General Information: Isolated from human feces. Methanogen that uses methanol and hydrogen to produce methane. This organism produces methane from methanol and hydrogen unlike most methanogens that use carbon dioxide and hydrogen.
Subject: NC_006177:3457637 Symbiobacterium thermophilum IAM 14863, complete genome
Lineage: Symbiobacterium thermophilum; Symbiobacterium; Shewanellaceae; Clostridiales; Firmicutes; Bacteria
General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.