Query: NC_007426:205390 Natronomonas pharaonis DSM 2160, complete genome Lineage: Natronomonas pharaonis; Natronomonas; Halobacteriaceae; Halobacteriales; Euryarchaeota; Archaea General Information: Isolated from Lake Gabara in Egypt. Extreme haloalkaliphilic archeon. Natronomonas pharaonis is able to survive at high salt and pH conditions which results in limited nitrogen availability through ammonium. In order to compensate for this, Natronomonas pharaonis has developed three systems to promote nitrogen assimilation: direct uptake of ammonia, uptake of nitrate, and uptake of urea. Another problem with high pH environments is the use of a proton gradient for the generation of ATP, which other alkaliphiles have adapted to by substitution of sodium ions for protons. However, this organism utilizes protons for ATP generation as determined by experimental data.
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General Information: This strain was isolated at Penn State University, USA and is used commercially for malolactic fermentation in wines. Lactic acid bacterium used in wine production. Oenococcus oeni is another member of the lactic acid bacteria and it occurs naturally in marshes and similar environments. It carries out malolactic conversion during secondary fermentation in wine production which is the conversion of malic acid to lactic acid with a concomitant rise in pH, making the wine microbiologically stable and enhancing the sensory properties of the wine (aroma, flavor, and texture). The organism's high tolerance to sulfite and ethanol mean that it will be the predominant organism in the wine at the end of fermentation where it cleans up the remaining sugars and converts the bitter-tasting malic acid.