Query: NC_021150:1965264 Azotobacter vinelandii CA6, complete genome Lineage: Azotobacter vinelandii; Azotobacter; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.
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General Information: Mycobacterium MCS was isolated from soil in a wood preservative-contaminated land-treatment unit where remediation of polycyclic aromatic hydrocarbon (PAH) was occurring. This isolate mineralizes small- and large-ring PAHs, in contrast to other PAH-degrading microbes. Bioremediation of PAHs offers an attractive solution to pollution clean-up because it can occur on site and at relative little cost compared to alternatives. This isolate belongs to a fast-growing group of the mycobacterium genus that is defined as Gram-positive, acid-fast, pleomorphic, non-motile rods. Bioremediation of soils contaminated with wood preservatives containing polycyclic aromatic hydrocarbons (PAHs) is desired because of their toxic, mutagenic, and carcinogenic properties. Creosote wood preservative–contaminated soils at the Champion International Superfund Site in Libby, Montana currently undergo bioremediation in a prepared-bed land treatment unit (LTU) process.