Pre_GI: SWBIT SVG BLASTN

Query: NC_020134:845164 Clostridium stercorarium subsp. stercorarium DSM 8532, complete

Lineage: Clostridium stercorarium; Clostridium; unclassified Ruminococcaceae; Clostridiales; Firmicutes; Bacteria

General Information: Lignocellulosic biomass has great potential as an abundant and renewable source of fermentable sugars through enzymic saccharification. Clostridium stercorarium is a catabolically versatile bacterium producing a wide range of hydrolases for degradation of biomass. Together with Clostridium thermocellum, Clostridium aldrichii and other cellulose degraders, it forms group I of the clostridia. It is moderately thermophilic, with an optimum growth temperature of 65 degrees C, and has repeatedly been isolated from self-heated compost. The two-component cellulase system of C. stercorarium has been investigated thoroughly. Due to its ability to utilize the various polysaccharides present in biomass it is especially suited for the fermentation of hemicellulose to organic solvents. Some isolates have been used in Japan in a single-step ethanol-fermenting pilot-process with lignocellulosic biomass as substrate.

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BLASTN Alignment.txt

Subject: NC_015709:1459838 Zymomonas mobilis subsp. pomaceae ATCC 29192 chromosome, complete

Lineage: Zymomonas mobilis; Zymomonas; Sphingomonadaceae; Sphingomonadales; Proteobacteria; Bacteria

General Information: Country: United Kingdom; Isolation: Sick cider; Temp: Mesophile. The natural habitat of this organism includes sugar-rich plant saps where the bacterium ferments sugar to ethanol. The high conversion of sugars to ethanol makes this organism useful in industrial production systems, particularly in production of bioethanol for fuel. A recombinant strain of this bacterium is utilized for the conversion of sugars, particularly xylose, which is not utilized by another common sugar-fermenting organism such as yeast, to ethanol. Since xylose is a common breakdown product of cellulose or a waste component of the agricultural industry, it is an attractive source for ethanol production. Zymomonas mobilis was chosen for this process as it is ethanol-tolerant (up to 120 grams of ethanol per litre) and productive (5-10% more ethanol than Saccharomyces). This bacterium ferments using the Enter-Doudoroff pathway, with the result that less carbon is used in cellular biomass production and more ends up as ethanol, another factor that favors this organism for ethanol production.