General Information: This is a thermophilic, facultatively mixotrophic sulfur-oxidizing bacterium. Thermus scotoductus SA-01 was isolated from fissure water in a South African gold mine. This organism is a thermophilic bacterium which was isolated from fissure water in the Witwatersrand Supergroup at a depth of 3.2 km below surface in a South African gold mine. It is a 2.9-billion-year-old formation of low permeability sandstone and shale with minor volcanic units and conglomerates. The ambient temperature of the rock is approximately 60°C. Samples were collected from a freshly mined rock surface and from a water-producing borehole that penetrated 121 m horizontally into the formation at a depth of 3,198 m. T. scotoductus SA-01 is a facultative anaerobe capable of coupling the oxidation of organic substrates to reduction of a wide range of electron acceptors, including nitrate, Fe(III), Mn(IV) or S(0) as terminal electron acceptors.