Pre_GI: SWBIT SVG BLASTN

Query: NC_016894:2809125 Acetobacterium woodii DSM 1030 chromosome, complete genome

Lineage: Acetobacterium woodii; Acetobacterium; Eubacteriaceae; Clostridiales; Firmicutes; Bacteria

General Information: Acetobacterium woodii is a Gram positive, motile, strict anaerobic, acetogenic bacterium, that relies on Na+ as coupling ion in bioenergetic reactions. The organism can use a wide range of substrates, such as sugars, alcohols, methoxylated aromatic acids or C1 compounds. Electrons derived from these electron donors are used in the Wood-Ljungdahl-pathway where the organism fixes CO2 and produces acetate. The pathway of CO2-fixation is coupled to energy conservation via a chemiosmotic mechanism, one enzyme that seems to be involved is the Rnf complex. The produced Na+ gradient can be used to drive ATP-synthesis or flagella rotation. The ATP synthase is a member of the F1FO class of enzymes and has an unusual hybrid rotor. Can use alternative electron acceptors like the lignin degradation product caffeate.

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BLASTN Alignment.txt

Subject: NC_009012:3591687 Clostridium thermocellum ATCC 27405, complete genome

Lineage: Clostridium thermocellum; Clostridium; Clostridiaceae; Clostridiales; Firmicutes; Bacteria

General Information: This strain is a well studied producer of endoglucanase and several restriction endonucleases. Thermophilic cellulose degrading bacterium. This genus comprises about 150 metabolically diverse species of anaerobes that are ubiquitous in virtually all anoxic habitats where organic compounds are present, including soils, aquatic sediments and the intestinal tracts of animals and humans. This shape is attributed to the presence of endospores that develop under conditions unfavorable for vegetative growth and distend single cells terminally or sub-terminally. Spores germinate under conditions favorable for vegetative growth, such as anaerobiosis and presence of organic substrates. It is believed that present day Mollicutes (Eubacteria) have evolved regressively (i.e., by genome reduction) from gram-positive clostridia-like ancestors with a low GC content in DNA. Some species are capable of producing organic solvents (acetone, ethanol, etc,), molecular hydrogen and other useful compounds. This organism is a thermophilic anaerobe that produces an extracellular enzyme system capable of degrading crystalline cellulose to soluble sugars that are further utilized as the carbon source for growth.