Pre_GI: SWBIT SVG BLASTN

Query: NC_014976:1174430 Bacillus subtilis BSn5 chromosome, complete genome

Lineage: Bacillus subtilis; Bacillus; Bacillaceae; Bacillales; Firmicutes; Bacteria

General Information: Bacillus subtilis BSn5 was isolated from Amorphophallus konjac calli tissue culture. Bacilllus subtilis BSn5 could inhibit Erwinia carotovora subsp. carotovora strain SCG1, which causes Amorphophallus soft rot disease and affects Amorphophallus industry development This organism was one of the first bacteria studied, and was named Vibrio subtilis in 1835 and renamed Bacillus subtilis in 1872. It is one of the most well characterized bacterial organisms, and is a model system for cell differentiation and development. This soil bacterium can divide asymmetrically, producing an endospore that is resistant to environmental factors such as heat, acid, and salt, and which can persist in the environment for long periods of time. The endospore is formed at times of nutritional stress, allowing the organism to persist in the environment until conditions become favorable. Prior to the decision to produce the spore the bacterium might become motile, through the production of flagella, and also take up DNA from the environment through the competence system.The sporulation process is complex and involves the coordinated regulation of hundreds of genes in the genome. This initial step results in the coordinated asymmetric cellular division and endospore formation through multiple stages that produces a single spore from the mother cell.

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BLASTN Alignment.txt

Subject: NC_012560:4311906 Azotobacter vinelandii DJ, complete genome

Lineage: Azotobacter vinelandii; Azotobacter; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.