Query: NC_014614:6115 Clostridium sticklandii, complete genome Lineage: Clostridium sticklandii; Clostridium; unclassified Peptostreptococcaceae; Clostridiales; Firmicutes; Bacteria General Information: This Clostridium (HF strain, ATCC 12662) was initially found associated with Methanococcus vannieli in formate-enriched cultures. After its isolation in pure culture, it was shown that this organism is capable of fermenting amino acids. Biochemical studies have shown that C. sticklandii obtains energy for growth from oxidation-reduction reactions between specific amino acid pairs. Clostridium sticklandii is a Gram-positive bacterium with low (G +C) content that is capable of fermenting amino acids.
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General Information: This strain was isolated at Penn State University, USA and is used commercially for malolactic fermentation in wines. Lactic acid bacterium used in wine production. Oenococcus oeni is another member of the lactic acid bacteria and it occurs naturally in marshes and similar environments. It carries out malolactic conversion during secondary fermentation in wine production which is the conversion of malic acid to lactic acid with a concomitant rise in pH, making the wine microbiologically stable and enhancing the sensory properties of the wine (aroma, flavor, and texture). The organism's high tolerance to sulfite and ethanol mean that it will be the predominant organism in the wine at the end of fermentation where it cleans up the remaining sugars and converts the bitter-tasting malic acid.