General Information: Strain B4 was isolated from a soil sample taken from the roadside in Hiroshima, Japan. It is a benzene-tolerant, Gram-postitive, non-motile, strictly aerobic rod, able to use many organic compound as its sole source of carbon and energy. Rhodococcus opacus is an aerobic, Gram-positive bacterium that can accumulate triacylglycerols (TAGs).