Pre_GI: SWBIT SVG BLASTN

Query: NC_012850:3454270 Rhizobium leguminosarum bv. trifolii WSM1325, complete genome

Lineage: Rhizobium leguminosarum; Rhizobium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Temp: Mesophile; Habitat: Host, Root nodule. This biovar is a symbiont of clover plants and is important commercially as it is used in the agricultural industry. Strain WSM1325 is compatible with many perennial clovers of Mediterranean origin used in farming, such as T. pratense, and is therefore one of the most important clover inoculants but is incompatible with American and African perennial clovers, such as those nodulated by the dissimilar strain WSM2304. This organism, like other Rhizobia, establishes a symbiotic relationship with a legume plant, providing nitrogen in exchange for a protected environment. The legume roots secrete flavonoids and isoflavonoids which the bacteria recognize and use to turn on genes involved in root nodulation. Many of the root nodulation genes are involved in synthesis and secretion of a nodule inducing signal, a lipochito-oligosaccharide molecule, which the plant recognizes, triggering nodule formation. The bacterium is endocytosed and exists inside a membrane bound organelle, the symbiosome, and fixes nitrogen for the plant cell while the host cell provides carbon compounds for the bacterium to grow on. The nitrogen fixation is important as it obviates the need for expensive and environmentally damaging fertilizer use.

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BLASTN Alignment.txt

Subject: NC_008314:1465643 Ralstonia eutropha H16 chromosome 2, complete sequence

Lineage: Cupriavidus necator; Cupriavidus; Burkholderiaceae; Burkholderiales; Proteobacteria; Bacteria

General Information: This strain (ATCC 17699; H16), formerly Alcaligenes eutrophus was originally isolated from sludge. Cupriavidus necator also known as Ralstonia eutropha is a soil bacterium with diverse metabolic abilities. Strains of this organism are resistant to high levels of copper or are able to degrade chloroaromatic compounds such as halobenzoates and nitrophenols making them useful for bioremediation. Other strains have been studied for their ability to produce polyhydroxybutyrates which have industrial application. Another strain is able to attack other bacteria and fungi when nutrients in the soil are low.