Pre_GI: SWBIT SVG BLASTN

Query: NC_012560:2906459 Azotobacter vinelandii DJ, complete genome

Lineage: Azotobacter vinelandii; Azotobacter; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.

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BLASTN Alignment.txt

Subject: NC_008544:615448 Burkholderia cenocepacia HI2424 chromosome 3, complete sequence

Lineage: Burkholderia cenocepacia; Burkholderia; Burkholderiaceae; Burkholderiales; Proteobacteria; Bacteria

General Information: This organism (HI2424; BCC1) was isolated as part of a study to find this organism in natural environments. Samples taken from fields that were planted with onions in New York in 1999 and 2000 were examined and it was discovered that isolates were the same as ones that can infect CF patients. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients.