Pre_GI: SWBIT SVG BLASTN

Query: NC_012560:1564500 Azotobacter vinelandii DJ, complete genome

Lineage: Azotobacter vinelandii; Azotobacter; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: This organism was first isolated from the soil in Vineland, New Jersey, although it is found worldwide. It is a large obligate aerobe that has one of the highest respiratory rates of any organism. Azotobacter vinelandii also produces a number of unusual nitrogenases which allow it to fix atmospheric nitrogen to ammonia, a compound it can then use as a nitrogen source. It protects the oxygen-sensitive nitrogenase enzymes through its high respiratory rate, which sequesters the nitrogenase complexes in an anoxic environment. This organism has a number of unusual characteristics. Under extreme environmental conditions, the cell will produce a cyst that is resistant to dessication and is surrounded by two capsular polysaccharide layers. This organism produces two industrially important polysaccharides, poly-beta-hydroxybutyrate (PHB) and alginate. PHB is a thermoplastic biopolymer, and alginate is used in the food industry. Alginate is also used by the pathogen Pseudomonas aeruginosa to infect the lungs of cystic fibrosis patients.

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BLASTN Alignment.txt

Subject: NC_008702:2039462 Azoarcus sp. BH72, complete genome

Lineage: Azoarcus; Azoarcus; Rhodocyclaceae; Rhodocyclales; Proteobacteria; Bacteria

General Information: This organism is a nitrogen-fixing obligate endophyte first isolated from the roots of Kaller grass in the Punjab region of Pakistan. These organisms are never found in root-free soil and colonize root tissues without causing disease. This strain has been shown to express genes involved in nitrogen fixation in root tissue and is thought to provide significant amounts of nitrogen to its host plant.