Pre_GI: SWBIT SVG BLASTN

Query: NC_011988:924721 Agrobacterium vitis S4 chromosome 2, complete genome

Lineage: Agrobacterium vitis; Agrobacterium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Agrobacterium vitis is the causative agent of crown gall disease in grapes. Agrobacterium are Gram-negative, motile, soil-dwelling plant pathogens with the species name given based on the disease phenotype associated with the bacteria. Disease causing Agrobacterium spp. possess a tumor-inducing (Ti) plasmid, which carries genes for the formation of opines. A segment of the Ti plasmid gets transferred to the plant cell and integrates into the host cell genome. The plant cell then starts synthesizing opines, which can be catabolized by Agrobacterium spp, but not by the plant cells.

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BLASTN Alignment.txt

Subject: NC_012850:4422966 Rhizobium leguminosarum bv. trifolii WSM1325, complete genome

Lineage: Rhizobium leguminosarum; Rhizobium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Temp: Mesophile; Habitat: Host, Root nodule. This biovar is a symbiont of clover plants and is important commercially as it is used in the agricultural industry. Strain WSM1325 is compatible with many perennial clovers of Mediterranean origin used in farming, such as T. pratense, and is therefore one of the most important clover inoculants but is incompatible with American and African perennial clovers, such as those nodulated by the dissimilar strain WSM2304. This organism, like other Rhizobia, establishes a symbiotic relationship with a legume plant, providing nitrogen in exchange for a protected environment. The legume roots secrete flavonoids and isoflavonoids which the bacteria recognize and use to turn on genes involved in root nodulation. Many of the root nodulation genes are involved in synthesis and secretion of a nodule inducing signal, a lipochito-oligosaccharide molecule, which the plant recognizes, triggering nodule formation. The bacterium is endocytosed and exists inside a membrane bound organelle, the symbiosome, and fixes nitrogen for the plant cell while the host cell provides carbon compounds for the bacterium to grow on. The nitrogen fixation is important as it obviates the need for expensive and environmentally damaging fertilizer use.