Pre_GI: SWBIT SVG BLASTN

Query: NC_011368:678291 Rhizobium leguminosarum bv. trifolii WSM2304 plasmid pRLG201,

Lineage: Rhizobium leguminosarum; Rhizobium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Nitrogen-fixing plant symbiont. This biovar is a symbiont of clover plants and is important commercially as it is used in the agricultural industry. Strain WSM1325 is compatible with many perennial clovers of American and African origin used in farming, and is therefore one of the most important clover inoculants but is incompatible with Mediterranean perennial clovers, such as those nodulated by the dissimilar strain WSM1325. This organism, like other Rhizobia, establishes a symbiotic relationship with a legume plant, providing nitrogen in exchange for a protected environment. The legume roots secrete flavonoids and isoflavonoids which the bacteria recognize and use to turn on genes involved in root nodulation. Many of the root nodulation genes are involved in synthesis and secretion of a nodule inducing signal, a lipochito-oligosaccharide molecule, which the plant recognizes, triggering nodule formation. The bacterium is endocytosed and exists inside a membrane bound organelle, the symbiosome, and fixes nitrogen for the plant cell while the host cell provides carbon compounds for the bacterium to grow on. The nitrogen fixation is important as it obviates the need for expensive and environmentally damaging fertilizer use.

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BLASTN Alignment.txt

Subject: NC_010552:293703 Burkholderia ambifaria MC40-6 chromosome 2, complete sequence

Lineage: Burkholderia ambifaria; Burkholderia; Burkholderiaceae; Burkholderiales; Proteobacteria; Bacteria

General Information: This strain was isolated from a cystic fibrosis patient. These bacteria present great versatility to adapt to diverse environments, and are capable of degrading pollutants in water and soil, and fixing atmospheric nitrogen. This species include strains isolated from cystic fibrosis patients as well as strains with potential use in biocontrol.