Pre_GI: SWBIT SVG BLASTN

Query: NC_010322:1336231 Pseudomonas putida GB-1 chromosome, complete genome

Lineage: Pseudomonas putida; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Proteobacteria; Bacteria

General Information: Pseudomonas putida strain GB-1, a fresh water, Gram-negative gamma-proteobacterium, is a genetically tractable, robust manganese (Mn) oxidizer, and as such, is an ideal model for unraveling the catalytic mechanism for, and the molecular regulation of Mn(IV) oxide production and its eventual accumulation on the cell surface at the onset of stationary phase. Since its isolation from Green Bay nearly 20 years ago by Ken Nealson’s group (then at the Center for Great Lakes Studies, Univ. Wisconsin-Milwaukee, USA), it has been the non spore-forming, model organism (along with the closely-related strain MnB1) for molecular genetic studies of Mn(II) oxidization, protein transport and biofilm formation and for biochemical studies on protein purification and Mn(III)-pyoverdine binding. Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. Pseudomonas bacteria are found in nature in a biofilm or in planktonic form. Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. As they are metabolically versatile, and well characterized, it makes them great candidates for biocatalysis, bioremediation and other agricultural applications. Certain strains have been used in the production of bioplastics.

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BLASTN Alignment.txt

Subject: NC_004547:1174650 Erwinia carotovora subsp. atroseptica SCRI1043, complete genome

Lineage: Pectobacterium atrosepticum; Pectobacterium; Enterobacteriaceae; Enterobacteriales; Proteobacteria; Bacteria

General Information: This strain (ATCC BAA-672) is a virulent blackleg isolated from the stem of a potato plant. Causative agent for blackleg and soft rot disease in potatoes. Formerly Erwinia, these organisms are plant-specific pathogens that invade the vascular systems of plants. Both Pectobacterium chrysanthemia and Pectobacterium carotovora cause soft-rot diseases of various plant hosts through degradation of the plant cell walls. Pectobacterium colonize the intercellular spaces of plant cells and deliver potent effector molecules (Avr - avirulence) through a type III secretion system (Hrp - hypersensitive response and pathogenicity). Avr proteins control host-bacterium interactions, including host range. Expression of the plant cell-wall-degrading enzymes is controlled through a quorum-sensing mechanism that quantifies the number of Pectobacterium bacteria through measurement of the concentration of small molecules (acyl homoserine lactones) produced by Pectobacterium. Pectobacterium atrosepticum is an environmentally widespread organism that causes blackleg and soft rot disease in potatoes. This organism produces pectolytic enzymes that destroy plant tissue and allow the bacteria to spread.