Pre_GI: SWBIT SVG BLASTN

Query: NC_009720:216589 Xanthobacter autotrophicus Py2, complete genome

Lineage: Xanthobacter autotrophicus; Xanthobacter; Xanthobacteraceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Alkene-degrading bacterium. Xanthobacter autotrophicus is a nitrogen-fixing methylotroph, commonly isolated from organic rich soil, sediment and water. This organism uses an alkene-specific monooxygenase to convert propene to epoxypropane. This monooxygenase is also able to catalyze the initial step in the cometabolism of chlorinated alkenes such as vinyl chloride and trichloroethene. The Xanthobacter autotrophicus alkene monooxygenase and other genes involved in alkene degradation are located on a 320 kb megaplasmid.

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BLASTN Alignment.txt

Subject: NC_012850:459315 Rhizobium leguminosarum bv. trifolii WSM1325, complete genome

Lineage: Rhizobium leguminosarum; Rhizobium; Rhizobiaceae; Rhizobiales; Proteobacteria; Bacteria

General Information: Temp: Mesophile; Habitat: Host, Root nodule. This biovar is a symbiont of clover plants and is important commercially as it is used in the agricultural industry. Strain WSM1325 is compatible with many perennial clovers of Mediterranean origin used in farming, such as T. pratense, and is therefore one of the most important clover inoculants but is incompatible with American and African perennial clovers, such as those nodulated by the dissimilar strain WSM2304. This organism, like other Rhizobia, establishes a symbiotic relationship with a legume plant, providing nitrogen in exchange for a protected environment. The legume roots secrete flavonoids and isoflavonoids which the bacteria recognize and use to turn on genes involved in root nodulation. Many of the root nodulation genes are involved in synthesis and secretion of a nodule inducing signal, a lipochito-oligosaccharide molecule, which the plant recognizes, triggering nodule formation. The bacterium is endocytosed and exists inside a membrane bound organelle, the symbiosome, and fixes nitrogen for the plant cell while the host cell provides carbon compounds for the bacterium to grow on. The nitrogen fixation is important as it obviates the need for expensive and environmentally damaging fertilizer use.