Query: NC_009051:419929 Methanoculleus marisnigri JR1, complete genome Lineage: Methanoculleus marisnigri; Methanoculleus; Methanomicrobiaceae; Methanomicrobiales; Euryarchaeota; Archaea General Information: Methanogen present in marine and freshwater sediments. Methanoculleus marisnigri was first isolated from sediment from the Black Sea, but has also been identified in freshwater sediments. This organism can use hydrogen, formate, and secondary alcohols, such as propanol and butanol, for methanogenesis.
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General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.