Query: NC_008826:170609 Methylibium petroleiphilum PM1 plasmid RPME01, complete sequence Lineage: Methylibium petroleiphilum; Methylibium; ; Burkholderiales; Proteobacteria; Bacteria General Information: Methylibium petroleiphilum strain PM1 (ATCC BAA-1232) was isolated in 1998 from the biofilter of a treatment plant in an oil refinery in Los Angeles, California, USA. Strain PM1 is capable of degrading aromatic compounds such as benzene, toluene and xylenes. Methyl tertiary-butyl ether-degrading bacterium. Methylibium petroleiphilum is a methylotroph (able to utilize reduced one-carbon compounds) able to degrade methyl tertiary-butyl ether (MTBE) under aerobic conditions. MTBE is a gasoline additive used as an oxygenate and to raise the octane number.
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General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.