Query: NC_008786:1298817 Verminephrobacter eiseniae EF01-2, complete genome Lineage: Verminephrobacter eiseniae; Verminephrobacter; Comamonadaceae; Burkholderiales; Proteobacteria; Bacteria General Information: This species was isolated from the kidney of the earthworm Eisenia foetida. Evidence based on curing experiments, Acidovorax-specific probes, and 16S phylogeny, indicate that earthworm egg capsules contain high numbers of the bacterial endosymbiont. Juvenile earthworms are colonized during embryonic development within the egg capsule, and failing this are not likely to acquire the symbiont by association with colonized adults or their bedding.
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General Information: This symbiotic and thermophilic bacterium was discovered by screening for thermostable tryptanophases in Japanese compost. Cultured growth of this organism requires the presence of another bacterial species, such as a Bacillus or Escherichia coli, which provides diffusable metabolites required for its growth. Pure cultures can be obtained by growing Symbiobacterium thermophilum in a bioreactor, separated from its symbiotic counterpart by a dialysis membrane. Because of its symbiotic nature, it cannot be cultured with conventional methods. Despite a negative reaction for gram stain, this species is placed with the gram-positive bacteria based on 16s phylogenetic analysis.