Pre_GI: SWBIT SVG BLASTN

Query: NC_008146:5106775 Mycobacterium sp. MCS, complete genome

Lineage: Mycobacterium; Mycobacterium; Mycobacteriaceae; Actinomycetales; Actinobacteria; Bacteria

General Information: Mycobacterium MCS was isolated from soil in a wood preservative-contaminated land-treatment unit where remediation of polycyclic aromatic hydrocarbon (PAH) was occurring. This isolate mineralizes small- and large-ring PAHs, in contrast to other PAH-degrading microbes. Bioremediation of PAHs offers an attractive solution to pollution clean-up because it can occur on site and at relative little cost compared to alternatives. This isolate belongs to a fast-growing group of the mycobacterium genus that is defined as Gram-positive, acid-fast, pleomorphic, non-motile rods. Bioremediation of soils contaminated with wood preservatives containing polycyclic aromatic hydrocarbons (PAHs) is desired because of their toxic, mutagenic, and carcinogenic properties. Creosote wood preservative–contaminated soils at the Champion International Superfund Site in Libby, Montana currently undergo bioremediation in a prepared-bed land treatment unit (LTU) process.

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BLASTN Alignment.txt

Subject: NC_018583:95388 Gordonia sp. KTR9 plasmid pGKT3, complete sequence

Lineage: Gordonia; Gordonia; Gordoniaceae; Actinomycetales; Actinobacteria; Bacteria

General Information: Isolated from soil at an explosives testing facility. Can transform hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a cyclic nitramine explosive. Many military ranges in the United States and Canada are contaminated with explosive and propellant residues. Of the nitroaromatic and nitramine explosives that exist on these sites, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) has a low sorption capacity and can migrate through soil into surface water and groundwater.