Query: NC_007681:1157402 Methanosphaera stadtmanae DSM 3091, complete genome
Lineage: Methanosphaera stadtmanae; Methanosphaera; Methanobacteriaceae; Methanobacteriales; Euryarchaeota; Archaea
General Information: Isolated from human feces. Methanogen that uses methanol and hydrogen to produce methane. This organism produces methane from methanol and hydrogen unlike most methanogens that use carbon dioxide and hydrogen.
Subject: NC_007426:205390 Natronomonas pharaonis DSM 2160, complete genome
Lineage: Natronomonas pharaonis; Natronomonas; Halobacteriaceae; Halobacteriales; Euryarchaeota; Archaea
General Information: Isolated from Lake Gabara in Egypt. Extreme haloalkaliphilic archeon. Natronomonas pharaonis is able to survive at high salt and pH conditions which results in limited nitrogen availability through ammonium. In order to compensate for this, Natronomonas pharaonis has developed three systems to promote nitrogen assimilation: direct uptake of ammonia, uptake of nitrate, and uptake of urea. Another problem with high pH environments is the use of a proton gradient for the generation of ATP, which other alkaliphiles have adapted to by substitution of sodium ions for protons. However, this organism utilizes protons for ATP generation as determined by experimental data.