Pre_GI: SWBIT SVG BLASTN

Query: NC_006396:1253873 Haloarcula marismortui ATCC 43049 chromosome I, complete sequence

Lineage: Haloarcula marismortui; Haloarcula; Halobacteriaceae; Halobacteriales; Euryarchaeota; Archaea

General Information: This organism was isolated from the Dead Sea and will provide information on the proteins necessary for adaptation to a high salt environment. Halophilic archaeon. Halobacterial species are obligately halophilic microorganisms that have adapted to optimal growth under conditions of extremely high salinity 10 times that of sea water. They contain a correspondingly high concentration of salts internally and exhibit a variety of unusual and unique molecular characteristics. Since their discovery, extreme halophiles have been studied extensively by chemists, biochemists, microbiologists, and molecular biologists to define both molecular diversity and universal features of life. A notable list of early research milestones on halophiles includes the discovery of a cell envelope composed of an S-layer glycoprotein, archaeol ether lipids and purple membrane, and metabolic and biosynthetic processes operating at saturating salinities. These early discoveries established the value of investigations directed at extremophiles and set the stage for pioneering phylogenetic studies leading to the three-domain view of life and classification of Halobacterium as a member of the archaeal domain. This organism is also know as "Halobacterium of the Dead Sea". Growth occurs in 1.7-5.1 M NaCl with optimum salt concentration of 3.4-3.9 M NaCl. The cytosol of this organism is a supersaturated salt solution in which proteins are soluble and active. This halophile is chemoorganotrophic and able to use a wide variety of compounds as sole carbon and energy sources.

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BLASTN Alignment.txt

Subject: NC_013665:1766158 Methanocella paludicola SANAE, complete genome

Lineage: Methanocella paludicola; Methanocella; Methanocellaceae; Methanocellales; Euryarchaeota; Archaea

General Information: This strain was isolated in Japan from rice paddy samples. Strain SANAE belongs to the Rice Cluster I (RC-I), a 16S rRNA and mcrA gene sequences-defined euryarchaeal lineage within Methanosarcinales and Methanomicrobiales. The RC-I group plays a key role in releasing methane from rice fields, which accounts for 10 to 25% of the global methane emissions.