Query: NC_003901:2533000 Methanosarcina mazei Go1, complete genome
Lineage: Methanosarcina mazei; Methanosarcina; Methanosarcinaceae; Methanosarcinales; Euryarchaeota; Archaea
General Information: Methanosarcina mazei Go1 (DSM 3647) was isolated from an anaerobic sewage digester in Germany. Anaerobic methane-producing archeon. This organism is a strictly anaerobic, non-motile, methane-producing archaeon. This organism can also aggregate forming large irregular shaped clumps of cells. Occasionally these aggregates can grow to 1000 microns or more in diameter. Growth occurs at pH 5.5-8.0, with optimum growth at pH 6.8-7.2. Growth occurs at pH 5.5-8.0, with optimum growth at pH 6.8-7.2. Can be found in decaying leaf litter, garden soil, sewage treatment sludge digestors, black mud, feces of herbivores and other urban waste and sewage products.
Subject: NC_006396:1380882 Haloarcula marismortui ATCC 43049 chromosome I, complete sequence
Lineage: Haloarcula marismortui; Haloarcula; Halobacteriaceae; Halobacteriales; Euryarchaeota; Archaea
General Information: This organism was isolated from the Dead Sea and will provide information on the proteins necessary for adaptation to a high salt environment. Halophilic archaeon. Halobacterial species are obligately halophilic microorganisms that have adapted to optimal growth under conditions of extremely high salinity 10 times that of sea water. They contain a correspondingly high concentration of salts internally and exhibit a variety of unusual and unique molecular characteristics. Since their discovery, extreme halophiles have been studied extensively by chemists, biochemists, microbiologists, and molecular biologists to define both molecular diversity and universal features of life. A notable list of early research milestones on halophiles includes the discovery of a cell envelope composed of an S-layer glycoprotein, archaeol ether lipids and purple membrane, and metabolic and biosynthetic processes operating at saturating salinities. These early discoveries established the value of investigations directed at extremophiles and set the stage for pioneering phylogenetic studies leading to the three-domain view of life and classification of Halobacterium as a member of the archaeal domain. This organism is also know as "Halobacterium of the Dead Sea". Growth occurs in 1.7-5.1 M NaCl with optimum salt concentration of 3.4-3.9 M NaCl. The cytosol of this organism is a supersaturated salt solution in which proteins are soluble and active. This halophile is chemoorganotrophic and able to use a wide variety of compounds as sole carbon and energy sources.