LOCUS NC_017179:5 18891 bp DNA linear BCT 04-SEP-2013 DEFINITION Clostridium difficile BI1, complete genome. ACCESSION NC_017179 SOURCE Clostridium difficile BI1, complete genome. COMMENT locus start: 1133510; locus end: 1152400 COMMENT n1_4mer:GRV/n1_4mer:RV = 2.682383; n0_4mer:D = 20.028271; n0_4mer:PS = 34.557599 FEATURES Location/Qualifiers source 1..18891 /organism="Clostridium difficile BI1, complete genome." gene complement(1..801) /gene="" /locus_tag="" /db_xref="1133510..1134310" CDS complement(1..801) /gene="" /product="inorganic polyphosphate/ATP-NAD kinase" /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL" gene 936..1808 /gene="" /locus_tag="" /db_xref="1134445..1135317" CDS 936..1808 /gene="" /product="ABC transporter ATP-binding protein" /translation="VFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE LMYYYVNEVRGCSI" gene 1796..2482 /gene="" /locus_tag="" /db_xref="1135305..1135991" CDS 1796..2482 /gene="" /product="ABC transporter permease" /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI KISKKNKFNY" gene 2534..3076 /gene="" /locus_tag="" /db_xref="1136043..1136585" CDS 2534..3076 /gene="" /product="hypothetical protein" /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA KGLYLVVGKNNDTWNANIME" gene 3155..3622 /gene="" /locus_tag="" /db_xref="1136664..1137131" CDS 3155..3622 /gene="" /product="regulatory protein" /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW" gene 4330..5466 /gene="" /locus_tag="" /db_xref="1137839..1138975" CDS 4330..5466 /gene="" /product="butyryl-CoA dehydrogenase" /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK" gene 5525..6307 /gene="" /locus_tag="" /db_xref="1139034..1139816" CDS 5525..6307 /gene="" /product="electron transfer flavoprotein subunit beta" /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII" gene 6326..7336 /gene="" /locus_tag="" /db_xref="1139835..1140845" CDS 6326..7336 /gene="" /product="electron transfer flavoprotein alpha-subunit" /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL AN" gene 7355..8152 /gene="" /locus_tag="" /db_xref="1140864..1141661" CDS 7355..8152 /gene="" /product="3-hydroxybutyryl-CoA dehydratase" /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG" gene 8250..9095 /gene="" /locus_tag="" /db_xref="1141759..1142604" CDS 8250..9095 /gene="" /product="3-hydroxybutyryl-CoA dehydrogenase" /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF YDYNK" gene 9182..10357 /gene="" /locus_tag="" /db_xref="1142691..1143866" CDS 9182..10357 /gene="" /product="acetyl-CoA acetyltransferase" /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR" gene 10552..11454 /gene="" /locus_tag="" /db_xref="1144061..1144963" CDS 10552..11454 /gene="" /product="pseudouridylate synthase" /translation="LFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP RTKEKLKFKADIPEDMKELISKLR" gene 11468..12472 /gene="" /locus_tag="" /db_xref="1144977..1145981" CDS 11468..12472 /gene="" /product="hypothetical protein" /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK" gene complement(12616..12843) /gene="" /locus_tag="" /db_xref="1146125..1146352" CDS complement(12616..12843) /gene="" /product="acyl carrier protein" /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA LEDEFGIEIPDEEAENFKCIGDICKYIEENK" gene 13015..13440 /gene="" /locus_tag="" /db_xref="1146524..1146949" CDS 13015..13440 /gene="" /product="hypothetical protein" /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI" gene 13488..13694 /gene="" /locus_tag="" /db_xref="1146997..1147203" CDS 13488..13694 /gene="" /product="hypothetical protein" /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC DDNIWIWVILIVLLLFNFDDGCCC" gene 13876..14055 /gene="" /locus_tag="" /db_xref="1147385..1147564" CDS 13876..14055 /gene="" /product="hypothetical protein" /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF LIVLLILCCCGGCLE" gene 14089..14289 /gene="" /locus_tag="" /db_xref="1147598..1147798" CDS 14089..14289 /gene="" /product="hypothetical protein" /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF LILLLITCSCGGFFDGEVGCGC" gene 14542..15558 /gene="" /locus_tag="" /db_xref="1148051..1149067" CDS 14542..15558 /gene="" /product="LacI family transcriptional regulator" /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT ERKK" gene complement(15681..16079) /gene="" /locus_tag="" /db_xref="1149190..1149588" CDS complement(15681..16079) /gene="" /product="hypothetical protein" /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE" gene 16291..17196 /gene="" /locus_tag="" /db_xref="1149800..1150705" CDS 16291..17196 /gene="" /product="hypothetical protein" /translation="LNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL YNINEQESEYNYNEKNTKTRAKKKR" gene 17674..18891 /gene="" /locus_tag="" /db_xref="1151183..1152400" CDS 17674..18891 /gene="" /product="hypothetical protein" /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC GPNHGSGKPSCHR" ORIGIN 1 ctataaaaat ttttcagata ctctatgcca aaactcatag tttgacattc taagtaattt 61 tatttttgca tcagatatac taacttttat attttcgatt tgtctaaatc tgtgctcaac 121 tccatcaacg acaatcaata cagaatcttc aaatctatat tctggtgcaa tctttataac 181 agattcattt gaacatatta tacttgatgt aaagcaccta tatgcattgg tatttatcgg 241 atgtaaaggt gtaatctgca taagttcaag atttatatca acaatactac ctccagcaga 301 atagttatag gctgttgaac ctgtggaagt agaaataatc ataccatctc cactaaaatt 361 ttgtatatgt ttattatcaa ggcttaaatt taggtgtata gtcctagact tgtctcctct 421 tataacaacc tcatttacag caagcatatt agaaccacta gtagttgtat atacttccgc 481 attaagaagt gacatttctt gaataatata atcttttttt gtataagctt caataaagct 541 atcaatctta tctggtaaaa tatcaggaaa aaaccccaga tgacctgtat ttattcccat 601 tatgggaatc tcaggaaaat cgaaatctct aactgttctt aaaaaagagc catcaccacc 661 aattgatata ataagctctg tgtctggata aatatctgaa tacacttcaa agccagcatt 721 gataagtttt ctagttaaga gttctttagt ctcaagggac ttatttaatt ggtttgtatt 781 tatagttata attcttttca tatattcacc tcttcatact ttgtattata acatagtttt 841 ataattctta atttataaat ttataaatga cctttgtagt gaatgtgtta aaatatgtat 901 aggtgggtat aaaaaaatgc ataagaggtg aagatgtgtt tagcataagg aatatcacaa 961 aaaaattagg aaaattcaag ttaaataata taaacttaga gttaaaggaa ggggatattg 1021 ttgggattat aggaccaaat ggttcgggaa aaacgacttt aataaaaatt ataatgggga 1081 ttattgatgc agatgaaggt gaaattgaac tttgtaatga aactattgaa aattctccaa 1141 taagctttaa aaacaatatt ggttttgtat atgattcttt acaattttat ccacatttaa 1201 aggttaagga atttagaaaa atagtttctt tattttataa gaattttgat agagaaaaat 1261 ttgatgaata ccttaataag tttgacatag aagaaaatat gcatattgaa aatttatcta 1321 aaggtcaatc tgagaagctt atgttatcaa gcgcacttag tcataatgct aagttattga 1381 ttttggatga accaactgct ggtatagacc cgattgtaag aactgaaata atgcagtact 1441 tacaagattt tgtgaaaaat ggaagtagta gtgtaataat ttctactcat aatactgata 1501 atttaattaa aattgctgat tatcttgttt ttataaatag aggaaatcag atttttactg 1561 ttaagaaaga attaatagaa caagaatata agataattag ggcaaataaa gcagagttag 1621 aagcaattaa agaaagtata gttggtgtta aagagtataa atattataat gaggcacttg 1681 ttaaagttgg agatagcttg aaagtaaaaa gcctattgat agaaattgat aagcataagg 1741 tcaaaaatcc tactatagaa gaactaatgt attattatgt aaatgaggtg agaggatgtt 1801 caatttaata agaaaagatt taataatagg aattagttca gatggtatcc gtaatttaaa 1861 gtacatattg ctattttttg tgttttattt tttccttaat tcaatttcat attatacagt 1921 aagtattgtt gttagttatt tgatatttat aaatacattt gagtgtgatt atgaaaatga 1981 ttcaaggatt tttataagga gtatgccagt tagtatagaa gatatagttt actcaaaata 2041 tttattaggt gtaggactaa taatttctgt aacaataatt gtgtccttac taagtaaatt 2101 gacatcacta gtgttcttta gaaatatggt tttaaatgat gtattttttt ctgtaaatat 2161 atttttagca attttgacga ttttactgcc tttaattttt aagtttggat atggaaaaat 2221 gaaagtttgt ggttttatag taagtatttt aatttacttt gtatatggaa gtcttttgaa 2281 gatgattagt atgattgtat atcaagtaaa gcattttaat tatagtaaag ttggaggagt 2341 atatctttca aattatgtaa ctgatatagc taatactaag tatataaatt tatatagtat 2401 gacattttta acaataataa tttttataat atcaatgtat ttttctatta agatttcaaa 2461 aaagaataag tttaattatt aaaattttaa ttaatattat agcacaatag cacaaaatat 2521 aaggagacta aaaatgaaga agtttataaa agtttcattt tttattttgg ggatttttac 2581 actattcata gctataaata aattagtata tagagatatg gtaagtgtca aagattatga 2641 aataaatcat ttagaggaaa ttgtttttga ttttgatagc aggagaagta gagatatcgg 2701 tactattgta agatttaaaa taagcaataa tagtaaatat agttataaat taaacagtgc 2761 taaaatgaag tttgaaaatt gtgtggaaaa taaaaaggga atgaataagt gttctgcata 2821 tgtaaatgta gatttattta atgaaaaaaa ttctataact gaaagtactc tgaaatatgg 2881 aataaaacca aataatcaag gttatgttga ttttgtaatt ccaaaaggac taaattttga 2941 ttataggtac tttaatgaat ctggtatgag tgtagaatat aaaggagaat atattgtcaa 3001 tattccaatg gcaaaaggac tttatctagt agttggaaaa aataatgata catggaatgc 3061 taatattatg gaataatttg aaaaataaat caattataga tataattaag aataatatga 3121 aatttataaa aattcagact aggaggggta tttaatgtca ataagtaatg taagagagta 3181 ttttaaacaa tttggcaagg aagacagtat attagaattt gaacaatcta gtgctactgt 3241 ggaacttgca gctgaagcag ctggggttat tccagcaaga atagctaaga cactctcatt 3301 taaaatagga gatgatgcaa ttttaattgt gacagctggt gatgctaaga ttgataataa 3361 gaagtataag gctgaattta attgtaaggc taaaatgttg acaccagaag aagtcttgga 3421 gtttactgga catgctattg gtggagtctg tccatttggt ttaaaaaatt ctataaaagt 3481 ctatttagat gattcaatga agagatttga tacagtattt cctgcatgtg gcagtagtaa 3541 ctctgcaata gaacttacat gtgaagaaat ggagaagttt tctaagagtg aaaaatgggt 3601 agatgtttgt aagaattggt aaaaaaatat agtaaactat agtaggaatt agtaaaaaac 3661 tacctctgta tacaatctat acagaggtag ttttttgtgt aaatatttat aaatctaaga 3721 ggaaaatcta atttaaacta ataatccaaa ataaagttaa aaaattctga ttttttttcg 3781 aaacaataaa aaaatgacaa aaaagtggaa aaaatgtaat aaatattgtt aaaaaaataa 3841 ttagaaatta ttgaaaaatt atcctttgag tgttactatt atagatgtaa gatatttaaa 3901 aataaaaaat agtcagataa aaatacgatt atttagctct attaaaataa tttttatacg 3961 ataaaaatct tctgtacatg gctaaaaaac agatgtacag acaatattta atgtttaata 4021 taccctaata taaatatcgt aaacaaatta ttttatctgt gaccagtgtg aaagaaatct 4081 tattctaact tgaaatttca caaaattcca aaaagcgaga ttttttttaa gtatagttga 4141 ttttaaatta acaatttatg attaaattaa tgtcataatt ggttagtata atcacatata 4201 aatatagagc gtagtttgat taaggttata aatttctcga cgtatattca aataccttat 4261 tatagatgtt gccgagattt taattaataa atgaaaataa aatatttata aaatactgag 4321 gaggctatta tggatttaaa ttctaaaaaa tatcagatgc ttaaagagct atatgtaagc 4381 ttcgctgaaa atgaagttaa acctttagca acagaacttg atgaagaaga aagatttcct 4441 tatgaaacag tggaaaaaat ggcaaaagca ggaatgatgg gtataccata tccaaaagaa 4501 tatggtggag aaggtggaga cactgtagga tatataatgg cagttgaaga attgtctaga 4561 gtttgtggta ctacaggagt tatattatca gctcatacat ctcttggctc atggcctata 4621 tatcaatatg gtaatgaaga acaaaaacaa aaattcttaa aaccactagc aagtggagat 4681 aaattaggag catttggtct tactgagcct aatgctggta cagatgcgtc tggccaacaa 4741 acaactgctg ttttagacgg ggatgaatac atacttaatg gctcaaaaat atttataaca 4801 aatgcaatag ctggtgacat atatgtagta atggcaatga ctgataaatc taaggggaac 4861 aaaggaatat cagcatttat agttgaaaaa ggaactcctg ggtttagctt tggagttaaa 4921 gaaaagaaaa tgggtataag aggttcagct actagtgaat taatatttga ggattgcaga 4981 atacctaaag aaaatttact tggaaaagaa ggtcaaggat ttaagatagc aatgtctact 5041 cttgatggtg gtagaattgg tatagcagca caagctttag gtttagcaca aggtgctctt 5101 gatgaaactg ttaaatatgt aaaagaaaga gtacaatttg gtagaccatt atcaaaattc 5161 caaaataccc aattccaatt agctgatatg gaagttaaag tacaagcggc tagacacctt 5221 gtatatcaag cagctataaa taaagactta ggaaaacctt atggagtaga agcagcaatg 5281 gcaaaattat ttgcagctga aacagctatg gaagttacta cgaaagctgt acaacttcat 5341 ggaggatatg gatacactcg tgactatcca gtagaaagaa tgatgagaga tgctaagata 5401 actgaaatat atgaaggaac tagtgaagtt caaagaatgg ttatttcagg aaaattatta 5461 aaatagataa atattaaaat atacacaata ggtcgatatt aaaatagata tggaggaagg 5521 atttatgaat atagtcgttt gtataaaaca agttccagat acaacagaag ttaaactaga 5581 tcctaataca ggtactttaa ttagagatgg agtaccaagt ataataaacc ctgatgataa 5641 agcaggttta gaagaagcta taaaattaaa agaagaaatg ggtgctcatg taactgttat 5701 aacaatggga cctcctcaag cagatatggc tttaaaagaa gctttagcaa tgggtgcaga 5761 tagaggtata ttattaacag atagagcatt tgcaggtgct gatacttggg caacttcatc 5821 agcattagca ggagcattaa aaaatataga ttttgatatt ataatagctg gaagacaggc 5881 gatagatgga gatactgcac aagttggacc tcaaatagct gaacatttaa atcttccatc 5941 aataacatat gctgaagaaa taaaaactga aggtgaatat gtattagtaa aaagacaatt 6001 tgaagattgt tgccatgact taaaagttaa aatgccatgc cttataacaa ctcttaaaga 6061 tatgaacaca ccaagataca tgaaagttgg aagaatatat gatgctttcg aaaatgatgt 6121 agtagaaaca tggactgtaa aagatataga agttgaccct tctaatttag gtcttaaagg 6181 ttctccaact agtgtattta aatcatttac aaaatcagtt aaaccagctg gtacaatata 6241 caatgaagat gcgaaaacat cagctggaat tatcatagat aaattaaaag agaagtatat 6301 catataattt aaggggggat aaagaatggg taacgtttta gtagtaatag aacaaagaga 6361 aaatgtaatt caaactgttt ctttagaatt actaggaaag gctacagaaa tagcaaaaga 6421 ttatgataca aaagtttctg cattactttt aggtagtaag gtagaaggtt taatagatac 6481 attagcacac tatggtgcag atgaggtaat agtagtagat gatgaagctt tagcagtgta 6541 tacaactgaa ccatatacaa aagcagctta tgaagcaata aaagcagctg accctatagt 6601 tgtattattt ggtgcaactt caataggtag agatttagcg cctagagttt ctgctagaat 6661 acatacaggt cttactgctg actgtacagg tcttgcagta gctgaagata caaaattatt 6721 attaatgaca agacctgcct ttggtggaaa tataatggca acaatagttt gtaaagattt 6781 cagacctcaa atgtctacag ttagaccagg ggttatgaag aaaaatgaac ctgatgaaac 6841 taaagaagct gtaattaacc gtttcaaggt agaatttaat gatgctgata aattagttca 6901 agttgtacaa gtaataaaag aagctaaaaa acaagttaaa atagaagatg ctaagatatt 6961 agtttctgct ggacgtggaa tgggtggaaa agaaaactta gacatacttt atgaattagc 7021 tgaaattata ggtggagaag tttctggttc tcgtgccact atagatgcag gttggttaga 7081 taaagcaaga caagttggtc aaactggtaa aactgtaaga ccagaccttt atatagcatg 7141 tggtatatct ggagcaatac aacatatagc tggtatggaa gatgctgagt ttatagttgc 7201 tataaataaa aatccagaag ctccaatatt taaatatgct gatgttggta tagttggaga 7261 tgttcataaa gtgcttccag aacttatcag tcagttaagt gttgcaaaag aaaaaggtga 7321 agttttagct aactaatttc ttggggaggg tattatgagt acaagtgatg ttaaagttta 7381 tgagaatgta gctgttgaag tagatggaaa tatatgtaca gtgaaaatga atagacctaa 7441 agcccttaat gcaataaatt caaagacttt agaagaactt tatgaagtat ttgtagatat 7501 taataatgat gaaactattg atgttgtaat attgacaggg gaaggaaagg catttgtagc 7561 tggagcagat attgcataca tgaaagattt agatgctgta gctgctaaag attttagtat 7621 cttaggagca aaagcttttg gagaaataga aaatagtaaa aaagtagtga tagctgctgt 7681 aaacggattt gctttaggtg gaggatgtga acttgcaatg gcatgtgata taagaattgc 7741 atctgctaaa gctaaatttg gtcagccaga agtaactctt ggaataactc caggatatgg 7801 aggaactcaa agacttacaa gattggttgg aatggcaaaa gcaaaagaat taatctttac 7861 aggtcaagtt ataaaagctg atgaagctga aaaaataggg ctagtaaata gagtcgttga 7921 gccagacatt ttaatagaag aagttgagaa attagctaag ataatagcta aaaatgctca 7981 gcttgcagtt agatactcta aagaagcaat acaacttggt gctcaaactg atataaatac 8041 tggaatagat atagaatcta atttatttgg tctttgtttt tcaactaaag accaaaaaga 8101 aggaatgtca gctttcgttg aaaagagaga agctaacttt ataaaagggt aaaaatctaa 8161 ttaaaaatat atttgctgtt aactagatta aatagtattt taatctagtt aacagaatat 8221 aaatactaaa ataacaggag gttataacta tgaaattagc tgtaataggt agtggaacta 8281 tgggaagtgg tattgtacaa acttttgcaa gttgtggaca tgatgtatgt ttaaagagta 8341 gaactcaagg tgctatagat aaatgtttag ctttattaga taaaaattta actaagttag 8401 ttactaaggg aaaaatggat gaagctacaa aagcagaaat attaagtcat gttagttcaa 8461 ctactaatta tgaagattta aaagatatgg atttaataat agaagcatct gtagaagaca 8521 tgaatataaa gaaagatgtt ttcaagttac tagatgaatt atgtaaagaa gatactatct 8581 tggcaacaaa tacttcatca ttatctataa cagaaatagc ttcttctact aagagaccag 8641 ataaagttat aggaatgcat ttctttaatc cagttcctat gatgaaatta gttgaagtta 8701 taagtggtca gttaacatca aaagttactt ttgatacagt atttgaatta tctaagagta 8761 tcaataaagt accagtagat gtatctgaat ctcctggatt tgtagtaaat agaatactta 8821 tacctatgat aaatgaagct gttggtatat atgcagatgg tgttgcaagt aaagaagaaa 8881 tagatgaagc tatgaaatta ggagcaaacc atccaatggg accactagca ttaggtgatt 8941 taatcggatt agatgttgtt ttagctataa tgaacgtttt atatactgaa tttggagata 9001 ctaaatatag acctcatcca cttttagcta aaatggttag agctaatcaa ttaggaagaa 9061 aaactaagat aggattctat gattataata aataagaatt tgggaattaa aagtttaaat 9121 aaaattgttt aaaaaacaat ttcggatata tgaaaaatct aatttaatgg gggtaatgaa 9181 tatgagagaa gtagtaattg ccagtgcagc tagaacagca gtaggaagtt ttggaggagc 9241 atttaaatca gtttcagcgg tagagttagg ggtaacagca gctaaagaag ctataaaaag 9301 agctaacata actccagata tgatagatga atctctttta gggggagtac ttacagcagg 9361 tcttggacaa aatatagcaa gacaaatagc attaggagca ggaataccag tagaaaaacc 9421 agctatgact ataaatatag tttgtggttc tggattaaga tctgtttcaa tggcatctca 9481 acttatagca ttaggtgatg ctgatataat gttagttggt ggagctgaaa atatgagtat 9541 gtctccttat ttagtaccaa gtgcgagata tggtgcaaga atgggtgatg ctgcttttgt 9601 tgattcaatg ataaaagatg gattatcaga catatttaat aactatcaca tgggtattac 9661 tgctgaaaac atagcagagc aatggaatat aactagagaa gaacaagatg aattagctct 9721 tgcaagtcaa aataaagctg aaaaagctca agctgaagga aaatttgatg aagaaatagt 9781 tcctgttgtt ataaaaggaa gaaaaggtga cactgtagta gataaagatg aatatattaa 9841 gcctggcact acaatggaaa aacttgctaa gttaagacct gcatttaaaa aagatggaac 9901 agttactgct ggtaatgcat caggaataaa tgatggtgct gctatgctag tagtaatggc 9961 taaagaaaaa gctgaagaac taggaataga gcctcttgca actatagttt cttatggaac 10021 agctggtgtt gaccctaaaa taatgggata tggaccagtt ccagcaacta aaaaagcttt 10081 agaagctgct aatatgacta ttgaagatat agatttagtt gaagctaatg aggcatttgc 10141 tgcccaatct gtagctgtaa taagagactt aaatatagat atgaataaag ttaatgttaa 10201 tggtggagca atagctatag gacatccaat aggatgctca ggagcaagaa tacttactac 10261 acttttatat gaaatgaaga gaagagatgc taaaactggt cttgctacac tttgtatagg 10321 tggtggaatg ggaactactt taatagttaa gagatagttt tagattataa tatttacaaa 10381 ttaaatattt aattatgaat ttaacctgta tgaataacta tttgttattc ttacaggttt 10441 ttatagttaa aaataattat tatctattaa acaaaaatag tgtaaaatat ataatatcat 10501 gattttgaag atataaaaag caggaagata taaaagaaag taggtaaaag tttgtttaaa 10561 aaagagaatc aaagatataa tcttatatcg tatacaaatg aagaagaaat gactttaaaa 10621 gaagttttat tggacaagct aaatttctca gttaggtcat tatcaaaaat gaagaaggaa 10681 aagagtgttt tagtaaatgg agtatataaa aaaccaagtt taaaggtgta tagtggtgat 10741 ttaatagaag taaaaataga cgaggaaaaa gcaaattttg aacctcaaga tttaaattta 10801 cagataattt atgatgattt tgatataatt atggtgaata aacctccatt tatggtagtt 10861 cacccaacaa aaagccacta tgataaaaca atagcaaatg gtattagtta ctatatagat 10921 aatcaaaaag aaaacgtaaa aataagattt gtaaataggt tagatatgaa tacttcaggg 10981 ttagttatag tagcaaaaaa tgcttatgca catcatactt tatctacagc aatgagtgaa 11041 aataaagttg aaaaaaagta tataacagta gttgatggaa ttataaaaga aaatgaggga 11101 actatagatg agccaattta tagacctaca gaagattcaa taaagaggat aatagatgaa 11161 agaggacagt catctgttac tcactataaa gtaatagaaa gactagaaaa tgcaactgta 11221 ttagaagtaa gcttagaaac tggtagaaca catcaaataa gagtacatat ggctcatata 11281 ggtcatggaa taataggtga tgagttgtat ggttatgtag atgaagagct aataaatagg 11341 caagcacttc atgcatacaa gctagagttt gaacaaccaa gaacaaaaga aaaattgaaa 11401 tttaaagcag atataccaga ggatatgaaa gagttaatat cgaaattaag ataatggggt 11461 gatatatatg ataatacata aatttataat acatgttttg gacaaaaata gtgatgttcc 11521 aatattaaat gactttgaag gcaaagttaa tcaagaagta gatggttttt tccagaaagc 11581 tataaaaaga attgcaaaag atgaggattt aagaaaaggt gtttttaaag attataatga 11641 caatttaatt aaaaactgtt gtgaacaaat aatatatgat gaaagtactt ttttgaaaaa 11701 ttctaaagaa attgcatctt acttgtttga cgttatgaaa attaatgctg cactagaatc 11761 ttgtgatttg gcaatttgct tatacactat taaagatgaa aagagtgtag caatactgaa 11821 attagattat aaaaagttgt acactcattc aattgaattt gtggatgata aatttaatat 11881 tcaaatggtt tcaaatgaaa ttggtatccc agagacttta agacaaaagc aaggagcttt 11941 gattagttta agtggtataa atgatgagtt tcatctaagg cttttagata aggatgctga 12001 aaaagaaggg tcagaatcta agtttgtaac agaattttta aatgctaaaa aaatagatga 12061 tgataagtac aagaccaagg tatttaaaaa tacagctgaa aattggataa ctaatgctct 12121 tagtaatgat ataaagcaag ctgaagatgt aagaagtata ttaaattata cactgaagga 12181 aaaacatgaa gttgatataa atgactttgt tgataattca attaaagatg atgagttaaa 12241 ggatagcttt aaagagcata tggaagaaaa aggtcttgat gaaagtttta gtatagataa 12301 aaaatgggtt gagaaaaagc ttaaaaaaag aagtataaaa acggataatg gttttgacat 12361 aaaagggaat ctaactgatt ttgaagaccc aatgaagtat actgtaaagc aaaatcaaga 12421 tggaactata gatataataa taaagaatgt tacattttat gaagaaaagt aatttatata 12481 cttagtatat aaattaaata taagagcgtg tattttatat attgtaaata aatgtaatct 12541 agtctgtgcg aactctaata taatttatta tgtagaagtt tacacagact atttatattt 12601 gtagacaaaa cgatactatt tgttttcttc gatatattta catatatcac caatacattt 12661 aaagttctca gcttcctcgt ctggtatttc tattccaaat tcatcttcaa gggccattat 12721 aatttcaaca gcatctaatg aatcagcttc taagtcgtcc attaaagatg cttccatagt 12781 tatctcatct aaattgtcta cccctaattg ttctgctatt atttctttta ttttctcaaa 12841 cattttaagc ctcctaaagc agttttaatt aattttaatg ttagtttaat ataatatcaa 12901 gaatttttca atatggaatc tataaaaaat aataaaattt tataaatgta gtaataaagt 12961 attatttgat gaataaactt tattgataac aaatataact taggaggaaa tgctatgaaa 13021 tatgtaggac tattactaac ttcagtagga atgtttttac ttatagcagt aaatttttat 13081 tataattcaa taactttaga tatgcaacgt atagaggatt atgtaatgga gaccaattta 13141 atccttgaag atgtagctga aaaggagagt tatgtatcta atgaaaaaga agactacata 13201 tcaaggttaa tgcatgtaaa gaaaggaata gaaaattcaa aaacatcctt cttgatagaa 13261 agatataagg agtacaaaat aaaatcaata gaaagtttaa tctacactat atcagaggag 13321 aaaaaagatt atctggatga agttgataga tacaataaac ttggtgaaaa agaaataaat 13381 aaactaatca ataaaaactt tttagaagta acttatctgt ctattactac atatatttaa 13441 ttaaagagac aaaacttagt ctcttaatga ttcaaggagg attagttatg acaaaattag 13501 aagacaatat ggaacgtttc tttggaggat gcgaaggatt ttttggtaat aaaatgttga 13561 taataatagt tatagtattc ttactattat gcacagacat tttagaagac ttattatgcg 13621 atgacaatat atggatatgg gttatattaa tagtattgtt attatttaac ttcgatgatg 13681 gttgttgttg ctaaaaaata ttgattatta aataggtaaa accagtaact tatacaagtt 13741 atggttttac cttttttagt tcaaattaaa aaatttaaaa aaatagtaaa aatacattag 13801 caaaatggaa accaaatgca tagtatatgt taggagcgaa aagctcaaca aaatatcaac 13861 aggaggtaac gtacaatgtt agatagattc tttggagaag aaggatgttt tggtggagga 13921 ggatggtgga taatagtact attcttccta ttcttagctt ttggagatag ctgggcagaa 13981 tttgacataa tggcatggat accattccta atagttttat taatactttg ctgctgtggt 14041 ggatgcttag aataagaaca cagttattaa aaaaaggagg gctttgttat gttagataga 14101 ttttttggag aagaaggatg ctttggtgga ggaggatggt ggataatagt actattcttc 14161 ctattcttag cttttgaaga ttgctggaga gacattgaca taatgtcatg gatacctttc 14221 ttaatattat tgctaataac ttgttcatgt ggtggattct ttgatggtga agtaggttgc 14281 ggctgctaat acttaaatca ttagaataaa aaaatgctgg tattttatac cagtattttt 14341 ttattttatt ttttaatatg atttaaatta agaatgaata ttattaaatt tgttgaatat 14401 agggtataat ataaaatata atgtatttaa atgtacttgc ttttatggta aaataaaaac 14461 ttatataaat gtagttaaaa taggaacctt caaaaatttg gttcagtttt agataaattt 14521 agtaaaataa ggaggatgaa gatgaaaggc aatataacga taaaagatgt tgctaaacaa 14581 gcaggagtgt caatatctac tgtatctaga gttataaatg attcaaaacc tgtaactgat 14641 gaagtcaaac aaaaagtttt agaggttata aaagagactg gatatatacc aaatccactt 14701 gctagaagct tagtaacaaa gaagagtcaa ttaatagggg taatagttcc agaagtttca 14761 gattcttttg ttaatgaggt gttaaatggg atagaagaag ttgctaaaat gtatgactat 14821 gatattcttt tagcgaatac atactctgat aaggaacaag aacttaagag tataaatcta 14881 ttgagagcaa aacaagtgga aggtatagtt atgatttcat ggatagttga acaagaacat 14941 atcaactata tacaaaattg tggaatacca gcgacatata taagtaaaac tgctagaaat 15001 tatgatatat atacagtaag tactagcaac gaagaagcta cttttgatat gacagagcat 15061 cttataaaga aaggtcatga aaagatagct tttataatga cgagtaaaga tgatactgtt 15121 ttagaaatgg aaagacttgc tggttatgag aaagcacttt caaataacaa tatagaatta 15181 gacaagagtt tgattaagta tggtggaact gattatgaga gtggatacaa tagtatgaaa 15241 gaactattag atgatggaat aatacctcat gcggcttttg taacaggtga tgaggctgcc 15301 ataggtgcta taaatgctat atgtgatgct ggatataagg ttccagaaga catatctgtt 15361 gcaggattta atgatgttaa gatagctaga atgtatagac ctaaacttac tacagtatat 15421 caacctctat acgatatggg agcagtagca ataagaatgg ttataaaatt aataaataag 15481 gaattaattg aaaataagaa aatagaatta ccttatagaa ttgttgatag agaaagtgtt 15541 acagaaagaa aaaaataaaa ctattaaaat caatcaggta gtgcctaagg cactacctga 15601 ttttttatgt caataatatc aaatttaata atagattaac taaaaataat tatctaatta 15661 cttaaatagt ttatttttat ctactcatga gggttagact ttttagtttt cttaaataaa 15721 cctttagatt ttttttgttg accacctggt gtattttttg gtgcaggtga atttgattta 15781 cctttagtag gtttagcctt cttagtttta gctggtttag tatctctttg attttctgca 15841 tctgagattt tttccattat attatctagt tttttgcgtt gattttcatc taaaaatgct 15901 ttgaattttg atactacttc atctcttcca tctaagttct tacctatttt tataagttcc 15961 tccataagtt cattttctga ttttcctttg taactatcag ccatcttttc tattttatcc 16021 ttgtcaatat ttttttgttt agccattttt tctaatgact ctgtatctaa ttttttcata 16081 aaaaaacccc tttctataaa tatatattga attatattca tactatttat atatagaaat 16141 aatattgaag tggtgaattg tgagtgatat ataatataaa taaaaattct tatagataat 16201 atatatgcac tgagctttaa aaatggaaca ttaaaatatg aaaaacatac tttaatatag 16261 ggcaattata taatataagg gggaattgtc ttgaataact tactattttt gattggagtg 16321 attgcattga gtaatggtaa tatctctata tttgatgata aaaataaatc aaaaggaaat 16381 aatagagtta gaaaaaacaa aaaaaatgca aatgataaac tttcacagtc tacacatggc 16441 aatttaaggt ttgatgcaaa tgatataaaa agaggtttaa ggctcatgga tttaagtgaa 16501 gaagatttag aaatgggaat ggaaataata acaagaacta aaaaatacat gtcaagagat 16561 gaaagaaaaa tactaataaa aatagaaagt gttttagatt tagttagagg aataaaaaaa 16621 ttaaataata tagatatggt agatacagaa gatgaaacgg attttttcag aaaaatggat 16681 agtgatgata aaaagaatat gatgataaag gaaattatag atgtattccc agaaaaaaga 16741 aaaaattcag ttgaaaaagc tataggaatg aaaaagaaga tagatttatt tgcagaatta 16801 tttttaccag atgactttgg tgaaggtgga tttagtttga gctctttggc aaatataaac 16861 aatcttggta gtatgaataa tctcaaattg ttaggaagtc ttttaagagg tgatgatagt 16921 aataatgatg atgaagacaa taatgatgag ttaaattatg aagacgaaga attagattgt 16981 gaagacaaag aattggatga tgatgagtta acagatgaag gtgaaatgga ggataatgaa 17041 gatgaattag tagaagataa gaacacaggc tatgaaaata tagaaaatga tgaagatata 17101 gtatacgatg aggaactata caacattaat gagcaagaat ccgaatacaa ttataatgaa 17161 aaaaatacta aaactagagc taagaaaaaa cgataattaa ctaaatacat aaagaaaaaa 17221 ggaagaaaaa gtaatgatag aataagatta tctcaattac tatataaaac cactttaaaa 17281 aattcaaaat ttattatata ataataatat gaaatcctga aacatacgat aaggcttttt 17341 taaattcaac ctacaaatga aatacgggag accgtgtctg gtaaattata acatgcctag 17401 ttagtttgga cgttaaaggt tactggcaag tcacccacct gggagtgcct agggtgtgaa 17461 tttacttagc caccggtgtt tcgggacaaa aaaagtgcta ctatttcaaa tagtagtact 17521 ttttttatta atatgcattt ttctactaaa tacgcctaat tattgataaa ttcaaaaagc 17581 aagtaacaat taagattata aaagcatagt attaattagg cttcaaaaag ccaaagactt 17641 aataagaatc gcttacaggg agggcaatga aaaatgcaag attataaaaa aaataaaaga 17701 agaatgatga atcagccaat gtctacaatg aatgaagaag aagtgtatac agatgaaata 17761 aattcagaag acatgagagg gtttaaaaaa tcacaccatc ataatggatg taatactgat 17821 aataagtgtg agtgccatga tgagtgtaat ccatgtaacc catgtaatcc atgtaaacct 17881 aacccatgca atccatgcaa acctaatcca tgtgatgaca aatgtgaatg ccatgacaag 17941 tgtaaatgtg attgtgaacc atgtgaaatg gattcagatg aatgttttga aaacaaatgt 18001 ggaccagaat gttgtaatcc gatatctcca agaaacttct ctgtatcaaa tgcagtgcca 18061 tttgcaatag aggctaatag aatatttgat actatgcaat tccaaacatt tacagatgca 18121 acaggtccaa atggagaacc attaactttt gaaacagaag tagtagaagt atttggttca 18181 attccaagtg caggtcaagc aagtgtaact atagaaaaaa tatgcttaag taatgatgga 18241 atagttatag acacaggaat gacaacttta gaagatttcg atttagaccc attaggagat 18301 atagtaggaa gaaactgtga aacaactttt gaatttgcag tttgtggaga aagaaacgct 18361 gagtgctgta gacaaggaaa aggcaaatca gtagcttata aacaaagagg attaactgta 18421 gcagttcgta atttagtact agagctaaga ggtagatgtg gatgtacaga gttcgttgca 18481 ttagctttcc cagcagttag agcaggaggt ggatgtaaga gaagagttga ttatgtagaa 18541 tttactttta acacactttc agcaccaata tgcttgccag ctgacggaag agctgttact 18601 ttaagacaag aatatcaaac taacttaact gtagattgta taggaaaatc tatattaaaa 18661 ttagaatgca acgaatgttg tgaacctttc tatgaattaa ttataccaaa tgatatagat 18721 ttagtacttt gcttacaaga aacagttagc acattaataa gtgaacaaat agtagtttta 18781 gcatcaccaa atccaatcca accaagactt gttgatactt tctctaaagt atgtgatttt 18841 tcgcaatgtg gacctaatca tggaagtgga aagccaagtt gccacagata g //