LOCUS NC_010723:4 18756 bp DNA linear BCT 25-AUG-2011 DEFINITION Clostridium botulinum E3 str. Alaska E43, complete genome. ACCESSION NC_010723 SOURCE Clostridium botulinum E3 str. Alaska E43, complete genome. COMMENT locus start: 298954; locus end: 317709 FEATURES Location/Qualifiers source 1..18756 /organism="Clostridium botulinum E3 str. Alaska E43, complete genome." gene 0..1067 /gene="buk" /locus_tag="buk" /db_xref="298954..300021" CDS 0..1067 /gene="buk" /locus_tag="buk" /product="butyrate kinase" /translation="MSYKLLIINPGSTSTKIGVYENEKELFEETLRHTNEEIKRYETI YDQFQFRKDVILNILKEKNFDITTLSAIVGRGGMLKPVEGGTYAVNDAMIEDLKVGVQ GPHASNLGGIIAKSIGDELNIPSFIVDPVVTDELDDVARLSGVPELPRKSKFHALNQK AVAKRYGKDSGKGYENLNLIVVHMGGGVSVGAHKHGKVVDVNNALDGDGPFSPERAGT VPVGDLIKMCFSGQYTESEVYTKIVGKGGFVGYLNTNDVKGVIDKMEAGDKECEKIYK AFLYQITKTIGEMAAALNGKVDQILLTGGIAYSPTLVPDLKSNVEWIAPVTVYPGEDE LLALAQGAIRVLDGEEKAKIY" gene 1453..2286 /gene="" /locus_tag="" /db_xref="300407..301240" CDS 1453..2286 /gene="" /locus_tag="" /product="HTH DNA-binding protein" /translation="LRSSLNITNKLTMLTEMENDSKFQILEYNDLNGATDLETAFGLN IINESNIKLKQIRIILDESSVKLESGLLSYMKGNIDIKSDIGGVFGLGKKFITSKLTG ETMFKPVYKGSGEIFLEPSFGHFALIELEDDEIIVDDGMFCACEDGIEVGASLQRNIS STFLGNEGLCQTKISGNGIVALEIPVPETEIFKCILIDDTLKVDGNFAILRTGNIEFS VEKSSKSITGAITSGEGFVNVYRGTGEVWLIPTKTIYDEMRTKSFKEMTKPSKERNTE S" gene 2369..2665 /gene="" /locus_tag="" /db_xref="301323..301619" CDS 2369..2665 /gene="" /locus_tag="" /product="putative TrpR homolog YerC/YecD" /translation="MSSSDSKIKSEELDLLFKGILELENIDECYNFFEDVATINEIKS LAQRFHVAMLLKSKKTYTEIAEVTGASTATISRVNRCLNYGSNGYKTILEKLEK" gene 2955..4298 /gene="glmM" /locus_tag="glmM" /db_xref="301909..303252" CDS 2955..4298 /gene="glmM" /locus_tag="glmM" /product="phosphoglucosamine mutase" /translation="MDRMFGTDGVRGIANTELTAQMAYNLGRAGAYVLTEGAHKPKIL VAKDTRISGDMLESALVAGILSVGAEAVILGVVPTPAVAYLTREYNADAGVMISASHN PVEYNGIKFFNNKGYKLSDELEDGIQKVIESDFEGVPSPIGIDLGRERIEVAALEDYT EFAKQTIPYNLKGMKIALDCANGASYKSAVKAFRDLGADVFVINDNPDGTNINKNCGS THPEELMDYVVKKGCDLGFAFDGDADRCLAVDENGKLINGDFILMLCANYLKEIGKLK DDTLVVTVMSNLGLDIACRGLGIKLEKTKVGDRYVLEEMTKDNYVLGGEQSGHVIFLD YNTTGDGLVTALQVASIVKKKEKTLSELCSVMKELPQVLVNATVPNDKKNIYLEDAEI VEAIKEIEAKLNGVGRVLIRPSGTEPLVRVMLEGENQVEIDEMAHGLANLILSKI" gene 4654..5067 /gene="" /locus_tag="" /db_xref="303608..304021" CDS 4654..5067 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MNIVPYNEYLLVMPNGDTKGFDDLELAKAYINIYYERVIDHKID KDDYNDATEIGAEEPRRNICTQLGVDEGRCEVYSIDNFIEVIRNNLVFDDEREEIISK LLAKEISFNVYDYNLDTILVDIESIDMMEPYGDAD" gene 5602..6111 /gene="ilvN" /locus_tag="ilvN" /db_xref="304556..305065" CDS 5602..6111 /gene="ilvN" /locus_tag="ilvN" /product="acetolactate synthase, small subunit" /translation="MRKYVLSVLVKNSSGVLSRVSGLFSRRGYNIDSITAGRTEDPLV SRMTITLSGDDDVLEQVKKQLNKLEDVIRVINITSDDSVYRELVLIKVKASGDERAAI NETAKIFRCKVVDVSPNTLTIELTGDENKISALIKLMEEYGIEEMVRTGITALQRGNS TIKNSIEEY" gene 6285..7544 /gene="leuC" /locus_tag="leuC" /db_xref="305239..306498" CDS 6285..7544 /gene="leuC" /locus_tag="leuC" /product="3-isopropylmalate dehydratase, large subunit" /translation="MGMTMTQKILASHAGLESVKAGQLIEVNLDLVLGNDITTPVAIN EFKKFGVDKVFNKSQIAIVPDHFTPNKDIKAAEQVKYVREFSNKMGIENFFEVGEMGI EHCLLPEKGLVVAGDVVIGADSHTCTYGALGAFSTGIGSTDMAAGMATGQTWFKVPSA IKFILKNKPAKWVSGKDIILHIIGMIGVDGALYKSMEFVGDGLNYLSMDDRFTMANMA IEAGGKNGIFPVDDKTVEYLKEHTEKEWKIYEADEDAEYDEVIEIDLSTLKPTVSFPH LPDNTRTIDNANRVNIDQVVIGSCTNGRISDLRIARGILKGKKVKKGIRCIVIPGTQK IYLQALEEGIIKDLIEAGAVVSTPTCGPCLGGHMGILAKGERCVSTTNRNFVGRMGHV ESEVYLASPAVAAASALTGKITDPELV" gene 7560..8045 /gene="leuD" /locus_tag="leuD" /db_xref="306514..306999" CDS 7560..8045 /gene="leuD" /locus_tag="leuD" /product="3-isopropylmalate dehydratase, small subunit" /translation="MSIKGRVFKYGDNVDTDVIIPARYLNTSNHKELASHCMEDIDKD FVNNVKDGDIIVANKNFGCGSSREHAPIAIKASGISCVIASTFARIFYRNSINIGLPI LECDEAVKNINDGDELEVDFSTGIIKNLSKNEKYKGEAFPEFMQKIIDNDGLIGYIRN K" gene 8215..9297 /gene="leuB" /locus_tag="leuB" /db_xref="307169..308251" CDS 8215..9297 /gene="leuB" /locus_tag="leuB" /product="3-isopropylmalate dehydrogenase" /translation="MKYNVAVIPGDGIGPDIVTEAIKVLNVVGEKFGHTFEYEYVMAG GCSIDKTGEALPKETLDICKASDAVLLGAVGGPKWDNPDAKVRPEQALLGLRSGMNLY CNLRPAVLYEPLKNASPLKDEIVKNGIDIAIVRELTGGIYFGERGRDEIEGVESAYDT ERYSTTEINRIVKIGFETAMKRNKKLTCVDKANILETSRLWRKVIGEISKDYPEVEVN YLYIDNASMQLVKDPTQFDVIVTSNMFGDILSDEASMITGSIGMLPSASLGEGTLGMY EPIHGSAPDIAGKGIANPLATILSAAMMLRYSFNLDNEAKLIESSVLSVLEEGYRTGD IMSEGKIKVGTNEMGDLVCKKINEGE" gene 9304..10962 /gene="ilvD" /locus_tag="ilvD" /db_xref="308258..309916" CDS 9304..10962 /gene="ilvD" /locus_tag="ilvD" /product="dihydroxy-acid dehydratase" /translation="MKSDSIKKGPGKAAQRSLLKALGLTNEEISRPIIGIVSSQNEII PGHMNLDKITEAVRKGVLMSGGTPLVVPTIGVCDGIAMGHEGMKYSLVTRELIADSIE CMVKAHAFDALVLIPNCDKIVPGMVMAALRVNVPAVVISGGPMLAGKHKGKDISLTTM FEAVGSYENGTMDEKELCDLEECACPTCGSCSGMFTANSMNCLCEVLGIALPGNGTIP AVFSERIRLAKKAGMAVMDMLKNDIKPRDIINERSIMNALKADMALGCSTNSVLHITA IANEAKVNMNLDIINDLSSKTPDLCKLAPASNIHIENLYAAGGITAIMNELSKKDILD LNCITVTGKTQGENIKGVTVKDYEVIRPIDNPYSENGGIAILRGNLAPDGAVVKRAAV LPKMLVHEGPARVFNSEEEANTAIFDKTINPGDVIVIRYEGPKGGPGMREMLQATAAI AGMGLDDSVALITDGRFSGATRGASIGHVSPEAASGGMIGLLENGDIISIDINNAKLE VKLSDEEIKRRKLNFKPLEPKVKEGYLSRYAKLVSSASEGAILK" gene 11002..12675 /gene="ilvB" /locus_tag="ilvB" /db_xref="309956..311629" CDS 11002..12675 /gene="ilvB" /locus_tag="ilvB" /product="acetolactate synthase, large subunit, biosynthetic type" /translation="MLLTGAEILIKSLVDEGIDTVFGYPGGAVLNIYDELYKYKEKIR HVLTCHEQAAAHAADGYARATGKVGVCIATSGPGATNLVTGIATAYMDSIPMVAITGN VAKPLLGKDSFQEVDITGITMPITKHNYIVKNIDDLQDVIREAFYIAEEGRKGPVLID IPKDITSNKTEYKKLIPKKVTKGIENIKEGSFDEAINYINNCKKPFIYAGGGIVSSEA TEELIKFAEKIGAPVATSLMCTSEFPFEHNLYTGMIGMHGTKASNIGATQCDLLIVLG ARFSDRVISNQSHIKNAKIIHIDIDPAEINKNIKVNSCIIGDLKYVLNKITPLVGKRE NKEWINFITSLKEENHIDESNNGELTPKFLFDKLNKLNDGSFIITTEVGQHQMWASQY FNYINRRSFLSSGGLGTMGYGFGAAIGAQIGKPDKKVFNIAGDGSFGMNCNEMATAVK NKLLLVVIVMNNNALGMVRQWQTLFYEKRYSETTLDRQTDYVKLAEAFGAKGFRVEKR EDLEEALKKAIASKEPALIDYVISNDKKVFPMVAPGAPINQIISEEDIN" gene 12696..13712 /gene="ilvC" /locus_tag="ilvC" /db_xref="311650..312666" CDS 12696..13712 /gene="ilvC" /locus_tag="ilvC" /product="ketol-acid reductoisomerase" /translation="MTKMYYEKDTDLNLLKGKTIAVIGYGSQGHAHALNAKESGCNVI IGLYEGSKSWGKAEAQGFEVFSTAEAAKKADIIMILINDELQAAMYKKDIEPNLESGN MLMFAHGFNIHFDQITAPKDVDVTMIAPKGPGHTVRSEYQLGKGVPCLVAVHQDATGR ALETALAYANAIGGARAGVLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLVEAG YDERNAYFECIHEMKLIVDLIYQSGFAGMRYSVSNTAEYGDYITGSKIITEETKKTMK KILKDIQDGTFAKDFLLDMSEAGGQAHFKAMRKLAAEHKSEAVGSEIRKLYCWNNEDK LINN" gene 13798..13917 /gene="" /locus_tag="" /db_xref="312752..312871" CDS 13798..13917 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MSFMDTVKVDQYCKNIYVKNIYVKYEIYKFLSQFYAQFL" gene complement(13997..15886) /gene="" /locus_tag="" /db_xref="312951..314840" CDS complement(13997..15886) /gene="" /locus_tag="" /product="ABC-type multidrug/protein/lipid transport system, ATPase" /translation="MSKDLKKISNQKRGPMGGHGPGPRMVLPGEKAKDFKGTLKRLLG YLKPRRFTLIFVFIMAILSTLFNILSPKVMGEATTTLFNGVTMKLQGIPGAKIDFYAL KNILILLTSLYLFSAFFGYLQQYFMAGVAQKTVQKMRKDVNEKLARLPLKYFDSNSHG DILSRVTNDIDNISSTLQQSLTQLITAIVTMVGILIMMLSISPIMTLICLITLPACIF ATRPIIKRSQKFFLKQQKELGKLNGHIEEMYTGHQIIKAFGHEEKAIDTFNEINDKLY QAGWKAQFVSGLMMPLMNFINNIGYVLVSVVGGLLVTKKYITIGDIQAFIQYSKQFTQ PINQTANIANIIQSTIASAERVFEILDEIEEIQDTNTSKVDEHTINNLQGSVKFDHVK FGYKEDSILIEDMNIDVKPGETIAIVGPTGAGKTTLVNLLMRFYEINDGKITIDGINI IDFNRGDLRTTFGMVLQDTWLFKGTIKENIAYGKENSTDEDVIKAAKAAHADHFIRTL PKGYDTLLNEDASNISQGQKQLLTIARAILANPRILILDEATSSVDTRTESYIQNAMT RLMEGRTNFVIAHRLSTIKDADLILVMNHGDVIEKGTHSELLAQKGFYSDLYNSQFNH DEEAV" gene complement(15879..17612) /gene="" /locus_tag="" /db_xref="314833..316566" CDS complement(15879..17612) /gene="" /locus_tag="" /product="ABC transporter, ATP-binding protein" /translation="MLKLFKNLKPYKFGILFIILLTACQAFTQLYLPKLMSNIVDKGI INGDINYILKIGTVMILVALLGSVAAVFSSLFSSKVAIGFSKDLRKNIFTKVETLSVG DFNQIGTASLITRTTNDINQIQQVLVMMLRMMLMAPIMCAGGIVMALTTNAKLSTVLL FAAPWVIVSIAIVSKKATPLFKSMQKKLDKLNLVLRENLTGIRVIRAFNRISHERIRF NEANDDLTNTSIKVNKIMAVMMPLMQLILNLTAVAIIWFGGIRIDQGSMQIGDLMAFI QYTMEIMFSLLLLSMMFVMIPRASASAVRLNEVLEIEPSIKDEYNDKVNTTLRGFVEF KNVSFKYSDSETPVLSNINFSSNPGETTAIIGGTGSGKSTLIGLLPRFYDVTDGEILV SGINIKDIPQHELRNKIGFVPQKAVLFTGTISENIKYGKDDATLDEIKHAAEIAQATD FISEMKDNFETVIDQGGNNVSGGQKQRLSIARAIIRKPEIYIFDDSFSALDFKTDAKL RAALKNETVNSTVLIVAQRVSTVMDADRIIVLDDGIIVGIGTHKELLKTCDVYKEIVS SQLSKEEIENE" gene complement(17666..18109) /gene="" /locus_tag="" /db_xref="316620..317063" CDS complement(17666..18109) /gene="" /locus_tag="" /product="MarR-family transcriptional regulator" /translation="LSYDLTHSIHGILVDIMRLHFSSMSSLIDENLHPGQPKLLMALY NKDGQSQRELSDVLHIKPATTNVMISRLEKGAFLRKEQDKCDQRATKIFLTEKGHTTI KNISKSLEYLDDICLKDFTFEEELTLRRLLSKLKDNLTLNCNNNN" gene 18498..18755 /gene="" /locus_tag="" /db_xref="317452..317709" CDS 18498..18755 /gene="" /locus_tag="" /product="GIY-YIG domain protein" /translation="MNYVYILECSDNTFYTGWTNDLKKRIKMHSLGKGAKYTRGRTPV KLLYYEVFEDKKEAMRREYKIKRLTRLQKEILIKNFDCTLL" ORIGIN 1 atgtcatata aattattaat tattaatcca ggatcaactt ctacaaaaat aggagtttac 61 gaaaatgaaa aagaattatt tgaagaaaca ttaagacata ctaatgaaga aataaagaga 121 tatgaaacta tatatgatca atttcaattt agaaaagatg taatattaaa tattcttaag 181 gaaaagaatt tcgatataac tactttaagt gcaatagttg gaagaggcgg aatgcttaaa 241 ccagtagaag gtggaactta cgctgttaac gatgcaatga tagaagattt aaaagtagga 301 gtacaaggtc ctcatgcttc aaatcttgga ggaataatag caaaatcaat tggtgacgaa 361 ttaaatatac catcatttat agtagatcca gtagttactg acgaattaga tgatgtagca 421 agattatcag gagttccaga acttccaaga aaaagcaaat tccatgcttt gaatcaaaaa 481 gcagtagcta agagatatgg taaagatagt ggaaaaggtt acgaaaactt aaacttaata 541 gttgtacaca tgggtggtgg agtttctgtt ggagctcaca aacatggaaa agtagttgac 601 gttaacaatg ctttagatgg tgatggtcca ttttcaccag aaagagctgg aacagtacca 661 gtaggtgatt taattaaaat gtgttttagc ggacaatata ctgaatcaga agtatatact 721 aagatagttg gaaaaggtgg atttgttgga tacctaaaca ctaatgatgt taaaggtgtt 781 atagataaaa tggaagctgg agataaagaa tgtgaaaaaa tatataaagc attcctttat 841 caaataacaa aaactattgg agaaatggca gctgcattaa atggaaaagt ggatcaaata 901 ttattaacag gtggaattgc atattcacca acacttgttc cagatttaaa atcaaatgta 961 gaatggatag ctccagtaac tgtatatcca ggagaagatg aattattagc attagcacaa 1021 ggtgctataa gagttcttga cggagaagaa aaagctaaga tttactagaa tatatgaaaa 1081 gaggtcatat caaaagaaat tttgatatga cctcttttaa tgtataagaa aatataaaat 1141 ttttaaacat agaaactgag taatatcaaa ggtttataat gtgaaaaata gtaaatgtat 1201 tgtcacaaag tgaacgttgc acagtaactt ttccataatt tagtttaata tatgtatata 1261 taaataagtg ctatctgtta taggtgtatt ttaaatatat tattaaattc agtacatatt 1321 ataattttag atttaaataa gttaatgttt gatttggaat gttatttatc aaagctatag 1381 tataaatgaa taataaacga taataaagtc aaggttaata aataattatt ataatattaa 1441 agggggaact accttgagaa gttctttaaa tataactaac aaattaacaa tgttaactga 1501 aatggagaat gattcgaaat ttcaaatttt agaatataat gatttaaatg gagctactga 1561 tttagaaaca gcttttggac taaatataat taatgaaagt aatataaagt taaagcaaat 1621 aagaataata cttgatgaaa gttctgtaaa actagaatca ggtcttttga gttacatgaa 1681 aggtaatata gatattaaga gtgatatagg tggagtattt ggtttaggta agaaatttat 1741 tacaagtaaa ttaactggtg aaacaatgtt caaacctgtt tataaaggtt caggagaaat 1801 atttttagag ccatcatttg gacattttgc tttaattgaa ttagaagatg atgagataat 1861 tgtagatgat gggatgtttt gtgcttgtga agatggaata gaagtaggtg catcattaca 1921 gagaaatata tcttcaactt ttttaggaaa tgaaggttta tgtcaaacta aaataagtgg 1981 taatggaata gttgcattag agataccagt tcctgaaact gaaatattta agtgcatatt 2041 aatagatgat acattaaaag tagatgggaa ttttgcaatt ttaagaactg gaaacattga 2101 attttcagta gaaaaatcat ctaaatctat tacaggtgct ataacaagtg gtgaaggttt 2161 tgttaatgta tataggggga caggagaagt atggcttatt ccaactaaaa ctatatatga 2221 tgaaatgaga actaaaagtt ttaaagaaat gactaagcct agtaaagaga gaaatacaga 2281 gagttaatgc ttttatatat taaagttgta aagaaaagaa gttaaagtta taatatttaa 2341 agatgaaata attaaagagg tgagagataa tgagttcgtc agattcgaaa attaaaagtg 2401 aagaattaga cttattgttt aagggaattt tagaacttga aaatatagat gaatgttata 2461 acttttttga agatgttgct acaataaatg aaataaaatc attagctcaa agatttcatg 2521 ttgctatgct tttaaagagt aaaaaaactt atactgaaat tgctgaagtt acaggagcaa 2581 gtacagctac tataagcaga gtaaacagat gcttaaatta tggtagtaac ggatataaaa 2641 caatattaga aaaactagaa aagtaaatta ttaaattttt gctataagta gttaaaaatt 2701 acttatagca tattttaatt taaatactaa catatataaa ttaaaaatat tcaagagata 2761 ataaaattac atttatttct taaatatatg aacatatgct taaaatattg tcaatattga 2821 tgaaaaacat tatactatta tataggttaa attaaaaaaa tcatcaatat aaatagagaa 2881 ttatcaaatt tacgattatt tcataataat atagtaatta gatatatgag ataataaaaa 2941 aagcgaggta aaaatatgga tagaatgttt ggaactgatg gagttagagg aattgctaat 3001 actgagttaa cagctcaaat ggcttataat ttaggtagag ctggtgcata tgttttaact 3061 gaaggtgcac ataaaccaaa aatattagta gctaaggata caagaatatc tggagatatg 3121 ttagagtctg ctttagttgc tggaatatta tcagttggag cagaagcagt aatattagga 3181 gtagttccaa caccagcagt tgcatattta acaagagaat ataatgcaga tgcaggtgta 3241 atgatatcag cttctcataa tccagtagaa tacaatggaa taaaattctt taataataaa 3301 ggatataaac tatcagatga attagaagat ggaattcaaa aagtaattga aagtgatttt 3361 gaaggagtac caagtccaat aggaatagac ttaggaagag aaagaatcga agttgctgca 3421 ttagaggatt atacagaatt tgcaaagcaa acaattcctt ataatttaaa aggaatgaaa 3481 atagcactag attgcgctaa tggtgcatct tataaatctg ctgtaaaagc atttagagat 3541 ttaggtgctg atgtttttgt aattaatgat aatccagatg gaactaatat aaataaaaat 3601 tgtggttcaa cacatccaga agaacttatg gattacgttg ttaaaaaagg ctgcgattta 3661 ggatttgcat ttgatggaga tgctgacaga tgtttagcag ttgatgagaa tggtaaatta 3721 ataaatggtg atttcatact gatgttatgt gcaaattatc taaaagaaat aggaaagtta 3781 aaggacgata ctttagttgt aactgttatg agtaatttag gtttagacat tgcatgtaga 3841 gggttaggaa taaagcttga aaaaacaaaa gttggtgata gatacgttct tgaagaaatg 3901 actaaggaca actatgtgct aggtggagaa caatcaggtc atgttatatt cttagattat 3961 aatacaacag gagatggact tgttacagca cttcaagttg catcaatagt taaaaagaag 4021 gaaaaaacat tatcagaatt atgttctgta atgaaagaat taccacaagt tttagttaat 4081 gcaactgttc ctaatgataa aaagaacata tacttagaag atgcagaaat agtagaagct 4141 attaaagaaa tcgaagcaaa actaaatggt gtaggaagag tcttaataag accatcagga 4201 actgaaccat tagttagagt tatgctagaa ggtgaaaacc aagtagaaat agatgaaatg 4261 gctcatggtt tagctaactt aattttatct aaaatataac tagtttatgt tttaatataa 4321 aattattata gataagcaaa catgaaatta ataaatctat aaagaaccat cacaaggtga 4381 ttaaaaatca ttttgtgatg ttttttttat ttcttataat tttaaaaaat aattatcttt 4441 aaatatattt aataagaata aagatgaatt atttattacc taaaacatta aatttttata 4501 ataatttaaa attgaaaaaa gtgtttcaaa tattaatttt aaaatatatg tagtaagaat 4561 aaaaagcaac ttttttgttt agagaatagc atctataaaa ctagggtata aataaggtga 4621 tgtctaaaag tacatgctaa ggagggaaaa acttatgaat atagttccat ataatgaata 4681 tttattagtt atgccaaatg gtgatactaa gggattcgat gatttagagt tagcaaaagc 4741 ttatataaat atatattatg aaagagttat agatcataaa attgataaag atgattataa 4801 tgatgcaaca gaaattggcg cagaagaacc tagaagaaat atatgcactc aattaggcgt 4861 agacgaagga cgctgtgaag tctatagtat agataatttc atagaagtta taagaaataa 4921 tttggttttt gatgatgaaa gagaagaaat aatatcaaaa ttattagcaa aggaaataag 4981 ctttaatgtc tatgattata atttagatac tatattagtt gatattgaaa gtatagatat 5041 gatggagcct tatggagatg cagattaagg ataaaaatct caaaaattat gtattattat 5101 tatgtttaat aaattgataa aataaaaatc attatgataa tatataatgt atattgatta 5161 aaaactatca ataaaatagc tactttattg actaattaga aaatatatat caaaaaatat 5221 tccttaatat ataagttttg taaaaataat ctacggttgt tgactttgat attttataag 5281 agtaaacttt aaaaaaagca aaagttaata tgtcttaatc attaaatgta tagatggaga 5341 cgattatata gcaagataag ttttcagaga atcggtggtt ggtgtgaatc gatactttag 5401 ctttatagtt atcactcctg agcagaatca cagaacaata gtatatatta ttaagtaagt 5461 gatgtccggt gtgcacccgt tatatgcaag ggtttgttag aacctagtga tattttatat 5521 aagcggaatt taaccacttt ttatgatttg tatcattaaa agtggttttt gttttaaata 5581 aattttacgg aggtatgatt ttatgaggaa atatgtatta tccgttttag taaagaattc 5641 atcaggtgta ttaagtaggg tttcagggtt attttcaaga cgaggatata atattgacag 5701 cataacagca ggaagaacag aagatccttt agtatctaga atgacgatta ctttaagcgg 5761 agatgatgat gttttagaac aagttaagaa gcaattaaat aaacttgaag atgttataag 5821 agtaataaat attacttctg atgattctgt ttatagagaa ttagtactta taaaagttaa 5881 agctagtgga gatgaaagag cagcaattaa tgaaaccgca aagatattta gatgtaaagt 5941 agttgatgtt tcaccaaata cattaactat agagttgaca ggtgatgaga ataaaatatc 6001 agcattaata aaacttatgg aggaatatgg aatagaggag atggttagaa caggtataac 6061 tgctcttcaa aggggaaatt caacaattaa aaattcaatt gaggaatatt aaaatatcta 6121 tttgaaatta aaaaaatatg ataggtaaag aaataagatt aatgattagt tattaaaaat 6181 ggggcaagct atgtttaatc aatttgttaa aaatttaatt caatactaaa tgatggggta 6241 caagataaac taggttaaaa acgtattttt aggaggatga taaaaatggg gatgacaatg 6301 actcaaaaaa tattagcgtc acatgcaggg ttagaaagtg tgaaagcagg tcaattaatc 6361 gaagtaaatc ttgatttagt tttaggaaat gatattacaa caccagtagc tataaatgaa 6421 tttaaaaaat ttggtgttga taaagtcttt aataaaagtc aaattgcaat tgtaccagat 6481 cactttactc caaataaaga tataaaagca gctgaacagg taaagtatgt tagagaattt 6541 tctaataaga tggggataga aaatttcttt gaagtaggag aaatgggaat agagcattgt 6601 ttacttcctg aaaaaggact agttgttgca ggggatgtgg ttattggggc tgattctcat 6661 acttgcacat atggtgctct tggagcattt tcaactggaa taggttcaac tgatatggca 6721 gcaggaatgg ctactggaca aacttggttt aaagttccgt cagctataaa atttattctt 6781 aaaaataaac cagctaaatg ggttagtgga aaagatatta ttttacacat aataggaatg 6841 ataggtgttg atggagcttt atataaatca atggagtttg ttggagatgg attaaattat 6901 ctttccatgg atgatagatt tacaatggct aatatggcaa ttgaagcagg tggaaaaaat 6961 ggaatattcc cagtagatga taagactgtt gaatatttaa aagaacatac agaaaaagaa 7021 tggaagatct atgaagcaga tgaagatgca gaatatgatg aagttataga aatagattta 7081 agcacactaa aacctacagt ttcatttcct catttacctg ataatacaag aactattgat 7141 aatgctaata gggtaaatat tgaccaagta gttattggat cttgtacaaa tggaagaata 7201 tcagatttaa gaatagcaag gggtatttta aaaggtaaaa aggttaaaaa aggaattaga 7261 tgcatagtta tcccaggaac tcaaaaaatt tatttgcaag cattagaaga aggtattatt 7321 aaagatttaa ttgaagcagg agcagtggtt tcaacgccaa cttgtggccc atgtttaggt 7381 ggtcatatgg ggatattagc aaaaggagaa agatgtgttt caacaacaaa tagaaatttt 7441 gtgggtagaa tgggtcatgt tgaatcagaa gtatatcttg ctagtccagc agtagcggca 7501 gcatcagctt taacaggaaa aataactgat ccagagttag tgtaagaggg gggattttat 7561 atgagcataa aaggtagagt atttaaatat ggtgataatg ttgatactga tgtaataatt 7621 ccagcaagat acttaaatac atctaatcac aaagaacttg catcacattg tatggaagat 7681 atagataaag attttgttaa taatgtaaaa gatggagata taatagttgc taataaaaat 7741 tttggttgtg gctcttcaag agaacatgca cctatagcga ttaaagcatc tgggattagc 7801 tgtgttatag catcaacatt tgcaagaatc ttttatagga attcaataaa cataggttta 7861 ccaatattag aatgtgatga agcagtgaaa aatattaatg atggagatga acttgaagtt 7921 gatttctcaa cagggataat taagaatcta agtaaaaatg agaaatataa aggtgaagca 7981 tttcctgaat ttatgcaaaa aattatagat aacgatgggc taatagggta tataagaaat 8041 aagtaatttc aatgagtaat aaggaataaa taaaaattaa ggcaataagt aaaattgata 8101 attgatacat tttatgggga tgaaataaat aatatacaac ttaaagaaat tttgaaagag 8161 ttatgctttt aattgtttat tataaattat caattaaaaa gaagggggac tatttatgaa 8221 atataatgtg gctgtaatac caggagatgg aataggtcct gatatagtaa cagaagctat 8281 aaaagtattg aatgtagttg gtgaaaagtt tggacataca tttgaatatg aatatgtaat 8341 ggctggaggt tgttctattg ataaaactgg agaagcactt ccaaaagaaa ctctagatat 8401 atgtaaagca agtgatgctg ttttgttagg tgctgtaggt ggaccaaaat gggacaatcc 8461 tgatgcaaaa gttagaccag aacaagcatt attaggatta agaagtggaa tgaatcttta 8521 ttgtaactta agaccagcag tattatatga acctctaaaa aatgcatcac cacttaaaga 8581 tgagatagtt aaaaatggaa tagatattgc tattgtaaga gagttaacag gtggtattta 8641 ttttggtgaa agaggaagag atgaaataga aggcgtagaa tcagcttatg atacagaaag 8701 atatagcact actgaaataa atagaatagt taaaattggt tttgaaacgg caatgaagag 8761 aaataaaaaa ttaacatgtg ttgataaagc aaatatactt gaaacttcta gactttggag 8821 aaaagtaatt ggggaaatat caaaagatta tccagaagtt gaggttaatt atttatatat 8881 agataatgcc tcaatgcaac ttgtaaaaga tccaacgcaa tttgatgtaa ttgtaacgtc 8941 taatatgttt ggggatattt tatctgatga agctagtatg attacaggct caattggtat 9001 gttaccatca gcatcattag gtgaggggac tttaggaatg tatgaaccaa ttcatggtag 9061 tgcacctgat atagcaggta agggaattgc aaatccatta gcaacaatat tatctgctgc 9121 tatgatgctt aggtatagtt ttaatttaga taatgaagca aaactaattg aaagttcagt 9181 attatcagta ttagaagagg gttatagaac tggagatata atgagtgaag gaaaaattaa 9241 ggttggaact aatgaaatgg gagatttagt ttgtaagaaa ataaatgagg gggagtaggg 9301 agttatgaaa agtgattcta taaagaaagg tcctggaaaa gcagcacaaa gatcactcct 9361 taaggcttta ggattaacaa atgaagaaat ttcaaggcct attataggaa tagtttcatc 9421 acaaaatgag atcattccag gtcatatgaa tcttgataaa ataactgaag cagtaaggaa 9481 aggagtttta atgtctggtg gtacaccact cgttgtgcct acaattggag tatgtgatgg 9541 tattgcaatg ggacatgaag gaatgaaata ttctttagtt acaagagaac ttatagcaga 9601 ttctattgag tgtatggtta aagcgcatgc ttttgatgca ttagttctca ttccaaactg 9661 cgataagatt gtaccaggaa tggttatggc agcattaaga gttaatgttc cagctgttgt 9721 tataagtgga ggaccaatgc ttgcaggaaa acataaagga aaagatattt ctttaactac 9781 aatgtttgaa gctgttggat catatgaaaa tggaacaatg gatgaaaagg aattatgtga 9841 tttggaagag tgtgcatgcc caacatgtgg ttcatgttca ggaatgttta cagctaattc 9901 aatgaattgt ttatgtgagg tattgggtat agctttacct ggaaatggaa ctattccagc 9961 agttttttct gaaagaataa gacttgctaa aaaagctgga atggctgtta tggatatgtt 10021 aaaaaatgat attaagccaa gagatattat aaatgaaaga agtattatga atgcacttaa 10081 ggctgatatg gcacttggat gttcaactaa tagcgtgctt catataacag caatagcaaa 10141 tgaagcaaag gttaatatga atttagatat aataaatgat ttatcatcaa aaacaccaga 10201 tctttgtaag ttagctccag cttcaaatat tcatattgag aatttatatg cagcaggtgg 10261 aattactgca ataatgaatg aactttctaa gaaagatatt ttagatttaa attgcattac 10321 tgttacagga aaaacacaag gagaaaatat aaaaggggta acagttaagg attatgaagt 10381 cattagacct atagacaatc catatagtga aaatggagga atagcaatac ttagaggaaa 10441 tttagctcca gatggtgctg ttgtaaaaag agcagcagtt ttaccgaaga tgttagttca 10501 tgaaggacca gctagagtat ttaattcaga agaggaagca aacacagcta tttttgataa 10561 aacaataaat ccaggtgatg taattgtaat aaggtatgaa ggtcctaaag gtggacctgg 10621 aatgagagaa atgcttcaag ctacagcagc aattgctggt atgggacttg atgattctgt 10681 tgcacttata actgatggaa gatttagtgg agctactagg ggtgcatcaa tagggcatgt 10741 ttctccagaa gcagcatcag gtggaatgat tggattattg gaaaatgggg atattatttc 10801 aatagatata aacaatgcaa aactggaagt taaattaagt gatgaagaaa ttaaaagaag 10861 aaaacttaac tttaaaccac tagaaccaaa ggtaaaagag ggatacttat ctagatatgc 10921 aaagcttgta agctctgcca gtgaaggtgc aatattaaag taataagtaa atagcaataa 10981 gtaaagcaaa ggggtgaata aaatgttatt aacgggggct gaaatattga taaagtctct 11041 tgttgatgag ggtatagaca cagtttttgg ataccctgga ggggctgttt taaatatata 11101 tgatgaatta tataaatata aagagaaaat tcgtcatgtt ttaacttgtc atgaacaagc 11161 agctgcacat gcagcagatg gatatgccag agcaactggt aaggttggtg tatgtatagc 11221 gacatcagga ccaggagcaa caaatttagt tacagggata gcaacagctt atatggattc 11281 aattcctatg gtagctatta caggaaatgt agctaaacca cttcttggta aagatagttt 11341 tcaagaggta gatataactg gtatcacaat gccaattaca aagcataact acattgtaaa 11401 aaatatagat gatcttcagg atgtaataag agaagcattt tatatagctg aagaaggtag 11461 aaaaggtcca gttttaatag atataccaaa ggatataacc tctaataaaa cagaatataa 11521 aaaattgatt cctaaaaaag taactaaggg aatagagaat ataaaagaag gttcatttga 11581 tgaggctata aattatataa ataattgtaa aaaaccattt atatatgctg ggggcggaat 11641 tgtttcttct gaagcaacag aagagctaat aaagtttgca gaaaaaatag gagcacctgt 11701 agctacttct ttaatgtgta cttctgagtt tccatttgag cataatttat atacaggaat 11761 gattggaatg catgggacaa aagcatcaaa tataggtgca acgcagtgtg acttattaat 11821 agtattaggg gcaagattta gtgacagagt tataagtaat caaagtcata ttaaaaatgc 11881 aaaaataatt catatagata tagatccggc agaaataaat aaaaatataa aagtaaattc 11941 atgtataatt ggtgatttaa aatatgtgtt aaataaaata acaccgttag tggggaaaag 12001 agaaaataaa gaatggatta attttataac ttctcttaaa gaagaaaatc acatagatga 12061 aagcaataat ggagaattaa caccaaagtt tttatttgat aagctgaata aactaaatga 12121 tggtagtttt ataattacaa ctgaagttgg acaacatcaa atgtgggctt ctcagtattt 12181 taattatata aatagaagaa gttttttaag ttcaggtgga cttggaacaa tggggtatgg 12241 ctttggagct gctataggtg ctcaaatagg taagccagat aaaaaagtat ttaatattgc 12301 tggagacgga agttttggaa tgaattgcaa tgagatggct acagcagtta aaaataaatt 12361 actattggta gttatagtta tgaataacaa tgcactagga atggtaagac aatggcaaac 12421 attattctat gaaaaaagat attcagagac aactttagat aggcaaacag attatgttaa 12481 acttgcagaa gcttttggtg ctaaggggtt tagagttgaa aaaagagaag atcttgaaga 12541 ggctttaaag aaagcaattg catcaaaaga acctgcttta atagattatg taataagtaa 12601 tgataaaaaa gtttttccta tggttgcacc tggggcaccc attaatcaaa ttataagtga 12661 agaagatata aattaaataa ttagggggac gttaaaatga caaaaatgta ttatgaaaaa 12721 gacacagatt taaatttatt aaagggaaag actattgcag taataggcta tggtagtcaa 12781 ggacacgctc atgcattaaa cgctaaagaa tcaggatgta atgtaattat aggattatat 12841 gaaggtagca aatcatgggg caaagcagaa gcacaaggat ttgaagtgtt tagcacagct 12901 gaggcagcaa aaaaagctga tataatcatg attcttataa atgatgaatt acaagcagca 12961 atgtataaga aggatattga accaaatctt gaatcaggaa atatgttaat gtttgctcat 13021 ggttttaata ttcattttga tcaaataaca gcacctaaag atgttgatgt aacaatgatt 13081 gctccaaaag gaccaggaca tacagtaaga agtgaatatc aattaggtaa aggtgttcct 13141 tgtcttgtag cagtacatca agatgcaact ggtagggctt tagaaactgc acttgcatat 13201 gcaaatgcaa ttggtggagc gagagctggt gttcttgaaa caacttttag aacagaaact 13261 gaaactgatt tatttggaga acaagcagtt ctttgtggtg gagtttgtgc tcttatgcaa 13321 gctggttttg aaacattagt agaggctgga tacgatgaaa gaaatgctta ttttgaatgt 13381 atacatgaaa tgaaattaat agtagatctt atttatcaat caggttttgc tggaatgaga 13441 tattcagttt caaatacagc tgaatatggc gattatatta caggatctaa gataattact 13501 gaagaaacta agaagactat gaaaaaaata cttaaggata ttcaagatgg aacttttgca 13561 aaagatttct tattagatat gtctgaagct ggaggacaag ctcactttaa agcaatgaga 13621 aaattggcag cagaacataa atcagaagca gttggtagtg aaatacgtaa actttattgt 13681 tggaataatg aagacaaact aataaataac taataaataa ctaataaatt atattaagat 13741 gtaatatgat tagaagaaga atatacaatt aaaaagtggt aaagaattaa gtggtatgat 13801 gagttttatg gatactgtta aagtagatca gtattgtaaa aacatatatg taaaaaatat 13861 atatgtaaaa tatgagattt ataaattttt atcacaattt tatgcacagt ttttgtagat 13921 aacaatttca tttattaaaa aagtgtggat aactttaaaa aatgtggatt cattaatgaa 13981 tccacatttt ttgtatttta aacagcttct tcatcatgat taaattgact gttgtaaaga 14041 tcagagtaga atcctttttg agcaaggagt tctgagtgtg tacctttttc tataacatct 14101 ccatgattca taactaaaat taaatcagca tctttaattg ttgataatct atgagctata 14161 acaaagttag ttctaccttc cataagtctt gtcatagcat tttgtatata actttcagtt 14221 cttgtatcaa cgctactagt tgcttcatct agtattagta ttctaggatt agcaagtata 14281 gctcttgcaa tagttaataa ttgtttttga ccttgtgata tatttgatgc atcctcattt 14341 aataatgtgt cataaccttt aggtagagtt cttataaagt gatcagcatg agcagctttt 14401 gcagctttta ttacatcctc atcagtagaa ttttcttttc cataagcaat attttcttta 14461 atagtccctt taaataacca agtatcttga agaaccattc caaatgtagt acgtaaatca 14521 ccacgattaa agtcaattat atttattcca tcaatagtta tttttccatc attaatttcg 14581 tagaatctca ttaaaagatt aactaatgtg gttttaccag caccagtagg tcctacgatt 14641 gctattgttt caccaggttt aacatctata ttcatatcct caattaatat agaatcttct 14701 ttatatccaa atttaacatg atcaaactta acagatcctt gtaaattatt aatagtgtgt 14761 tcatctactt tagatgtatt ggtatcttga atttcttcta tttcatctaa gatttcgaat 14821 acacgttcag cagatgcaat agtagattga atgatattag ctatatttgc agtttgattt 14881 attggttgag tgaattgttt tgaatactga ataaatgctt gaatatcacc aatagttata 14941 tattttttag taaccaataa tccaccaaca actgaaacta atacatatcc tatattattt 15001 atgaaattca ttaagggcat cataagacca gatacaaatt gagccttcca accagcttga 15061 taaagtttat catttatctc attaaatgta tcaatagcct tttcttcatg tccaaaagct 15121 ttaataattt gatgaccagt atacatttct tcaatatgac catttaattt cccaagttct 15181 ttttgttgtt ttaaaaagaa cttttgagaa cgctttatta ttggtcttgt ggcaaatata 15241 caagcaggta acgtaattaa acaaatcaaa gtcattatag gactgatgct aagcatcatt 15301 ataagtattc caaccatagt aacaatagct gtgataagtt gtgttaaact ttgttgtaaa 15361 gtactactta tattatcaat atcattagta actctactta aaatatcacc atgagaattt 15421 gaatcaaaat attttaaagg taatctggct aatttttcat ttacatcttt acgcattttt 15481 tgaacagttt tttgtgcaac tccagccata aagtattgtt gcaaatatcc aaagaatgca 15541 ctaaataaat ataaagaagt aagaagaatt aaaatatttt ttaaagcata aaaatctatt 15601 tttgcgcctg gaatcccttg tagtttcatg gttacaccat taaataaagt agtagtagct 15661 tcacccatta ctttagggct tagtatatta aataatgtac ttaatatagc cattataaaa 15721 acaaaaataa gagtaaatct tctaggtttt aaatatccaa gtagtctttt tagtgttcct 15781 ttaaaatctt tagctttttc accaggtaaa accatacggg gtccaggtcc gtgtccaccc 15841 ataggtccac gtttttgatt agatatcttt ttaagatctt tactcatttt ctatctcctc 15901 ctttgaaagc tgagatgaaa ctatttcttt atatacatca caagttttta atagttcttt 15961 gtgagttcca atacctacaa taattccgtc atcgagtaca ataattctat cagcatccat 16021 aacagtacta actctttggg ctacaattaa aacagtagaa ttaacagttt cattttttaa 16081 agcagctcta agttttgcat cagttttaaa atctaatgca gaaaaactat catcgaaaat 16141 atagatttca ggttttctaa taatagcacg ggctattgat aaacgttgtt tttgacctcc 16201 agaaacatta ttgccaccct gatctattac tgtttcgaag ttatctttca tttctgagat 16261 aaagtcagta gcttgagcaa tttcagcagc atgtttaatt tcatctaatg tggcatcatc 16321 tttaccatat ttaatatttt cagagattgt tccagtaaac agtacagcct tttgaggtac 16381 aaatcctatt ttatttctta attcatgttg tggaatgtct tttatattaa ttccagaaac 16441 taatatttca ccatcagtta catcataaaa tctaggtaaa agtcctatta aagttgattt 16501 accagagcct gttccaccta taattgctgt agtttcacca ggattagaag aaaaattaat 16561 attacttaag acaggtgttt cagaatcaga atatttgaaa gacacatttt tgaattctac 16621 aaatcctctt aaggtagtat taaccttgtc gttatactca tcttttatag agggttctat 16681 ttcaagaact tcatttaatc ttacagcaga agcagaagct cttggtatca taacaaacat 16741 catagaaagt aataataatg agaacattat ttccattgtg tattgaataa aggccattaa 16801 atcaccaatt tgcatacttc cttgatcaat tcttattcca ccaaaccata ttattgctac 16861 agcagttaga ttaagaatta attgcataag aggcatcata acagccatta ttttatttac 16921 ctttattgat gtatttgtaa gatcatcatt agcctcattg aatctaattc tttcatgaga 16981 aatacgatta aatgctctaa ttactctgat tccagtaaga ttttctctta agactaaatt 17041 taatttatct agcttttttt gcattgattt aaataatggg gtagcttttt tactaacgat 17101 agctatagaa actattaccc atggtgctgc aaacaataaa acagtagaaa gctttgcatt 17161 tgtagttaat gccattacta ttccaccagc acacattatt ggagccataa gcatcatacg 17221 taacatcatt actaatactt gttggatttg atttatatca ttggtagttc tagtgattaa 17281 tgaagctgta ccaatttgat taaaatcccc aacagataga gtttctactt tagtaaagat 17341 attttttctt aaatcttttg aaaagccaat agcaacttta gatgaaaata aacttgaaaa 17401 aacagcagca acactaccta aaagagctac taatatcatt actgttccta tttttagtat 17461 gtaattaata tctccattaa ttattccttt atctactata ttggacatga gtttaggtaa 17521 atataattgt gtaaaagctt ggcatgctgt taataaaata ataaataata taccaaactt 17581 atatggtttt aaattcttaa ataatttcag catttatttt cacatccttc aaatatattg 17641 tttagaatat gctaaacaaa tagtatttaa ttattattat tacaattaag cgtaagatta 17701 tcctttagtt tactcagtaa tcttcttaat gttaattcct cttcaaaagt aaaatcttta 17761 aggcatatgt catctagata ttctagtgat ttacttatgt tttttatagt tgtatgtcct 17821 ttttcagtaa gaaatatttt agttgctctt tgatcacact tatcttgctc ttttcttaaa 17881 aatgctcctt tttctaatct agatatcata acgtttgttg ttgcaggttt aatatgcaaa 17941 acatctgaaa gttctctttg actttgtcca tccttattat ataaagccat taataatttg 18001 ggttgaccag ggtgtaaatt ttcatcaatt aaggaactca tagaagaaaa atgtaatcgc 18061 attatatcta ctaatattcc atgtattgaa tgtgttaaat cataagacaa aatatcatct 18121 ccaattttaa atttagttag ttgactaact aatattataa tactaaaaat gtaatattca 18181 atactaaaag taaataaatt acaatgaaat tatattttgt gaaatgtgta tttgtataca 18241 gttatagtag tgaaaaaatg atgatatttc atgtaattat aaataataat agttataaag 18301 ttaattatat ttatgaaaaa tataattagc tggctaagta tatttataga taaaaagttt 18361 aataaaataa agtgagaatt gtgttaatac atataaataa gtattatatt aatgcattat 18421 ttatcatata aaaatattat gcactttatg ttataatagt gactgataat atataataaa 18481 attttgagga atttatatat gaattatgtt tatattttag aatgttcaga taatactttt 18541 tatactggat ggacaaatga tttaaaaaaa agaataaaaa tgcattcttt aggaaaaggt 18601 gcaaaatata cacggggaag aactccagtt aaacttttgt attatgaagt gtttgaagat 18661 aaaaaagagg ctatgagaag agagtataaa ataaaaaggt taacgagatt acaaaaagaa 18721 atattgataa aaaattttga ctgtacttta ttatag //