LOCUS NC_010723:15 17945 bp DNA linear BCT 25-AUG-2011 DEFINITION Clostridium botulinum E3 str. Alaska E43, complete genome. ACCESSION NC_010723 SOURCE Clostridium botulinum E3 str. Alaska E43, complete genome. COMMENT locus start: 1133163; locus end: 1151107 FEATURES Location/Qualifiers source 1..17945 /organism="Clostridium botulinum E3 str. Alaska E43, complete genome." gene 0..563 /gene="" /locus_tag="" /db_xref="1133163..1133726" CDS 0..563 /gene="" /locus_tag="" /product="RNA polymerase sigma-70 factor, family" /translation="MINENTLIYRFYNKNPMKNDLVKMAMKGDSKAFTSLIRENKEYL YKMAYSYVKNEQMALDILQESIYKAFVNIKKLKRENVFKTWITKILINECINTIRKNA KVTYLNNDILIKNESISIDEKLDLYEAIDQLRDGYKTVIILKYFNDLPLKDISYIMDI PENTVKSHLKRAKNDLSKILKEDFLNE" gene 556..1626 /gene="" /locus_tag="" /db_xref="1133719..1134789" CDS 556..1626 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MNKNYENISIPNNIDDFIDVGVKRAVDLKNIKRKKLIRRKLGIT AASLIIILTIGFSNPAMAAKIPILGNVFQAIEKNIYYPSNYSEYSTSVNETSYSNGVG VTLSDILCDGQSLYVTYNIESDKPFKYTSWGDGPLTMNQLITSEKYNKVDFSHKELDN TGFAGLEGKFIDDNTFVGMEKYKLSSLKTSIPDEFIFQVKYASFETHSLEDDEKRDIK SGTWAFKIPVKVNKDIKRVVNLDNMGNDSVTLNSLSITPFDMIFSLTYNKGKWNDYEV SIYDENGEELNLSDSSIDEENKTENIIFKAPSNESKSIRIIINKPILEKGETITDSEG QCETSYEELGKELIFDEIINIK" gene 2037..2597 /gene="" /locus_tag="" /db_xref="1135200..1135760" CDS 2037..2597 /gene="" /locus_tag="" /product="membrane protein, DedA family" /translation="MMNTDVFINIMSNYSNIALLISIFASVIIAVLGVVPSIFVTGAN IIFFDPIKGFFISLIGETIGAAISFWLYRKGFKKKIENFSVNNKYLKKLVKSKGKEST YLVFLIRLLPFVPSGFVTLGASVSNMNIVPFTIATFLGKIPSIILEALVSYDFINIQD NWIRLIITLLALILIMIFMNKNYKSN" gene 2756..3682 /gene="" /locus_tag="" /db_xref="1135919..1136845" CDS 2756..3682 /gene="" /locus_tag="" /product="conserved protein, tetratricopeptide repeat family protein" /translation="MENKNYLYDNYLNMARNLKSERNLLKALKFYKKAYALNIGKTDI EFLLDMALLYDEIGLGKEAEEKYLEIINLDEDEPRAYYGLGTIHDELGNLSKAKEYYK ISIQKDSSYDKAYFFLANIYDELGDKEKAILNYKKTIELNNKDLWGYANLACIFEELD KNKEALKYINKALEINPKHYRILFNKAVISNKLHKKQEAIDYYKKSIKANPTYTYSFL NLAVIYREDNDFTTAINIINKGIEINPEEGFLYYNRACFYVNTKELLKALKDVEKCIE LNDLFLDYMKKDKELDPIRDLKEYKEFINNIT" gene 3866..4369 /gene="" /locus_tag="" /db_xref="1137029..1137532" CDS 3866..4369 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MKETFTEGLEISMDNLYKTGAFLTCGNENKANTMTISWGSVGYI WRRPIFMALVRETRYTKEFLDSGDNYTISIPFEGSMKSSLTICGTKSGRDVDKEKEAN IKFLPSKSVSSPIVHGCNKYYECKIMFKQEMDLDNIDPEIKDKFYGEGESKHVLYFAE ILESYTK" gene complement(4489..5139) /gene="" /locus_tag="" /db_xref="1137652..1138302" CDS complement(4489..5139) /gene="" /locus_tag="" /product="putative lipoprotein" /translation="MVKKLILATVLMLSIALVGCSSKNSATNTKTEDNTVVENSKTDN NENDIKKDTPTESSKDEKNDNKKETEAFSIYTEDANSMEIKEISTIELNKNLSLEDKL KQLSKTLSEKVFDKLPIEVKSIETIDGKKIATINLDESAANKGVTSNADLKKPNWTAD KLQGSTGGEITENSLLETFLQVKYKGEWIDGVKFLYKNEPIEFEHTPNLSQINYRK" gene 5390..5839 /gene="" /locus_tag="" /db_xref="1138553..1139002" CDS 5390..5839 /gene="" /locus_tag="" /product="MarR-family transcriptional regulator" /translation="MDQSKKIVNEILVELFNDILQIEEQTIREGVISDLSITEIHTIE AIGMYKKRTMSEVAQDLKITVGTLTTAINKLIKKGYVERSRIEEDRRVVLIALTKKGK LAYRLHEKFHNDMVSNSIESLTQQEEEMLISALNKINEFLKQKYKLK" gene 5854..6822 /gene="fabH" /locus_tag="fabH" /db_xref="1139017..1139985" CDS 5854..6822 /gene="fabH" /locus_tag="fabH" /product="3-oxoacyl-(acyl-carrier-protein) synthase III" /translation="MNIKIGGVGGYLPSLAVTNDKISEFVDTNDEWIVERTGIKERRI SEGENTSEIAVKAAKVALERAKIDSENLDLIIVATITPDMFMPSVACLVQKELNANNA TAFDINVACSGFVFALETANSLMKSLNYKNALVIGAETLSKVINWNDRGTCILFGDGG GAAVLVRDEKNGIINSYLKTDGKKGDSLTIGALDFNTPFTKENFISNQYVEMNGREVF KFATSAIIKAIDGALEGTDICLDDIKYIVPHQANIRILDYVAKKLKIDINKFYTNLDK TGNTSSASVPLALNEVYEKGLLNEGDKILLVAFGGGLSYGATLLEW" gene 6937..7161 /gene="" /locus_tag="" /db_xref="1140100..1140324" CDS 6937..7161 /gene="" /locus_tag="" /product="acyl carrier protein homolog" /translation="MLFEEIREIICEQLGVEKDGITLETTFEDLGADSLDLFQVVIEL EEKFDIQIEEVEKLKSLKDAVEYVKNKTNN" gene 7271..8212 /gene="" /locus_tag="" /db_xref="1140434..1141375" CDS 7271..8212 /gene="" /locus_tag="" /product="putative enoyl-(acyl-carrier-protein) reductase II" /translation="VANNRVCDLLGIKYPIFQGGMAWIADASLAAAVSEAGGIGIITG AAPTEWVRNQIREAKKLTDKPFGVNIMLMSENAEEIAELVCEENVAIVTTGAGSPGKY MERWKANNIKVIPVVASVALAKRMQKAGADAIIAEGTESGGHVGQLTTMALIPQVVDA VSIPVIAAGGIGDGRGVAASFMLGAEGVQVGTRFLVARECTVNQNYKDKILKAKDIDT EVTGRSTGHPVRVLKNKLSRMYNKLEKEGISSEKLEELGAGTLKKAAVDGDIENGSIM SGQIAGLINKEQTSKEIIEEMFEEAQQRFMLFGGNHE" gene 8205..9146 /gene="fabD" /locus_tag="fabD" /db_xref="1141368..1142309" CDS 8205..9146 /gene="fabD" /locus_tag="fabD" /product="malonyl CoA-acyl carrier protein transacylase" /translation="MNSENIAFLFPGQGAQYVGMAKEFYDKIPECKEIVDKAEKILDM PIKDMLFNGPEEVLMQTENAQPTIVVASLVALKALEVNNIEAKYTAGLSLGEYASLIF SGALSLEEGLLLVKERGRIMGSSLPKGLGKMAAILKLDNEKLEELLNRASEFGVVEAA NYNCPKQVAIAGENKAIDEAVKIARELGGLGIPLKVSGPFHSSLLKSASEEFYKTLKD TNINSLNKVTYSNVKGLPYDEKDDIKELLKKHIRSSVLFDKTIENMIKSGVNTFIEVG PGKVLSGFVKKINRTVKVLNIEDMESLNKLLLTLEEI" gene 9167..9907 /gene="fabG" /locus_tag="fabG" /db_xref="1142330..1143070" CDS 9167..9907 /gene="fabG" /locus_tag="fabG" /product="3-oxoacyl-[acyl-carrier-protein] reductase" /translation="MLKGKCAIITGAARGIGKAIALKLASLGANIVLNYRSSEEEAKI VASEIEKMGVEVLTVKGDISKLEDVENIISEAKNKFGIIDIIVNNAGITKDTLILRMK EKDFDDVIDVNLKGVFNCLKSITPVMVKQRHGKIINISSVVGVAGNAGQVNYAASKAG VIGMTKSLAKELGARGINVNAVAPGFIETDMTSVLGEKVKEEAKKNIPLKRFGTPEDV AGVVAFLASENSNYVTGQVINIDGGMVM" gene 9925..11166 /gene="fabF" /locus_tag="fabF" /db_xref="1143088..1144329" CDS 9925..11166 /gene="fabF" /locus_tag="fabF" /product="3-oxoacyl-[acyl-carrier-protein] synthase 2" /translation="MERRVVVTGMGALTPIGNDVDTFWKNNKEGKLGIDFIKLIDNEL INVKIAGELKDFNPEEVLGKKECKRLDRFSQMALIAADEAIKSSKIDLEKVDKKRFGV MVGSGIGGFATIETECTKLVTGKSKRMSPFFVPMAIINLAAGNISIQYGLEGSCTSVV TACATGTNNIGDAFRSIKHGYADFMLAGGAEAPITRIGVEGFHSMKALNSSNDPIKAS TPFDKNRSGFVMGEGAGILLLESLDSALERGANILGEIVGYGSTCDAYHITSPHPEGF GAAEAMSQAINEAKIDKTEVSYINAHGTSTQLNDKFETAAIKKVFGEDAYNIPISSTK SMTGHLLGAAGAIESIVCLKALEEGFVPPTIGYETKDEELDLDYVPNKGRKKELKYAL TNSLGFGGHNATLLFKRWEEK" gene 11178..11672 /gene="accB" /locus_tag="accB" /db_xref="1144341..1144835" CDS 11178..11672 /gene="accB" /locus_tag="accB" /product="acetyl-CoA carboxylase, biotin carboxyl carrier protein" /translation="MDFQNIKELINIINSSDLAYFELKSNDGYVKMDKSLTRSLNRNV EEEETTKKESNIHTKEEPIQEKNNFPENKSSNMQEEAVEEDTVIITSPMVGTFYSSPS PESESFAKEGDYVKKGKVICIIEAMKLMNEIESNYNGKIVKCFAKDGDMVEFGQKLFE IKED" gene 11678..12106 /gene="fabZ" /locus_tag="fabZ" /db_xref="1144841..1145269" CDS 11678..12106 /gene="fabZ" /locus_tag="fabZ" /product="beta-hydroxyacyl-(acyl-carrier-protein) dehydratase FabZ" /translation="MLKIEEIKEILPHRYPFLLIDRVTEMDIEEKLFVKGYKNVSANE QFFQGHYPQEPIMPGVLQIEALAQAGAVAILSMEKFKGKTPLFAGTNKVRFKAKVVPG DRLDLYCEIVKLKGPIGIGKGIASVDGKTVCEAEILFAIG" gene 12116..13468 /gene="accC" /locus_tag="accC" /db_xref="1145279..1146631" CDS 12116..13468 /gene="accC" /locus_tag="accC" /product="acetyl-CoA carboxylase, biotin carboxylase" /translation="MFKKVLIANRGEIAVRIIRACREMGILTVAVYSEIDKEALHTQL ADEAICIGPAIAKDSYLNIANILSACVLSGADAIHPGFGFLSENAKFAKMCKECNIKF IGPDYESIELMGDKARAREIMKKANVPVVPGYEGEIENETHALGIAKKIGYPIMIKAA AGGGGKGIRIARSDEEFLKGYKTAKSESKACFGDDRLYIEKFIEKPRHIEFQILADEY GNVVHLGERECSLQRKNQKVLEEAPSNILNEEIRNTMGEIAKKAAKAVNYKNAGTIEF LFDKDNNFYFMEMNTRIQVEHPITEMITGVDLVKEQLKIASGEKLLLKQEDIKISGHA IECRINAEDPENDFRPCPGEVSELCVPGGMGVRVDSAIYCGYNIPYFYDSMIAKLITF GKTREEAIVKMKRALGEFAVGGVKTNIDFQFSILEMQEFLDGNYDTSFLSEKMVKNNA" gene 13461..14336 /gene="accD" /locus_tag="accD" /db_xref="1146624..1147499" CDS 13461..14336 /gene="accD" /locus_tag="accD" /product="acetyl-CoA carboxylase, carboxyl transferase, beta subunit" /translation="MLKDLFKKSKYATVNPSAYKSKVVEEKPNIPSGMWTKCSNCNSM IYYEDLENNKYVCTKCNQHFRISPKERIKQIFDKDTFKEMWKSLKTNNPIDFEDYEEK INKSQSNTGSKEAVVTGVGRINDLEVACAIMDSFFMMGSMGTVVGEKITRIVEYATEN NLPILIFTASGGARMQEGIFSLMQMAKISAALARHDEKGLLYITILTDPTTGGVTASF AMEGDIILSEPNTLVGFAGRRVIENTINETLPESFQKSEFLLEKGFIDSIVERKNMRA YLYKILILHGVNKYE" gene 14329..15153 /gene="accA" /locus_tag="accA" /db_xref="1147492..1148316" CDS 14329..15153 /gene="accA" /locus_tag="accA" /product="acetyl-CoA carboxylase, carboxyl transferase, alpha subunit" /translation="MNKEFIKSIVVSSPWEKVEIARHKDRPTGKYYIDNIFKDFIEFH GDRLFGDDKAIIGGIASFEDISVTVIAITKGANTNENIERNFGMPNPEGYRKALRLMK QAEKFNRPVICFIDTPGAFCGVGAEERGQGSAIANNLFELSRLKTPIISIVIGEGGSG GALALTVADKILMLENAVYSILSPEGFASILWKDSKRVKEAANVMKITAQDLNEFGII DTVIKEPRGGAHKNPQKQVTLIKKEIMNAMNEMKNIETNQMINERYDKFRKIGTLE" gene 15335..16276 /gene="" /locus_tag="" /db_xref="1148498..1149439" CDS 15335..16276 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MDVDLKSKVDIFKDYYKDISRTLIFDNNKVKVLESLLYIINKNE VDKSELKSIRRYISKSELNSVFNGNVKKAMSAFLCSNFQYENIVKDTIIIYDNLVNKG FKKDKYLELLSFIITLRFHKKEYDNILNNMLKAKTEIKSKIQGIDEFKLMNICFINLS MFSREYDQLVRKYLKIYFKLLRKNFDKKEAFFIVLAMINLDEIDYTSYINNVIEIKSH LNNTKMEIKEEHYLLLGLSSLIIENNSEFVNNISEVYNELCEKRAIKKDQLVLVSITL VLYEYIEIISEDDEDLFTENEINIVKKLIEYLTVLLI" gene 16310..16723 /gene="" /locus_tag="" /db_xref="1149473..1149886" CDS 16310..16723 /gene="" /locus_tag="" /product="HutP superfamily" /translation="MESNSTRVAKIATKMAICDRFEEDDLKKIYNKEGIKVTAVNIGG NINSSISKILESALVASKRNGLIREEHLHEGAVIGATRDAIIQVSNRANAQNVGGKIG IARGGEHISVCIFLSIGLLHLDEVVIGIGHRAIPE" gene 17144..17944 /gene="" /locus_tag="" /db_xref="1150307..1151107" CDS 17144..17944 /gene="" /locus_tag="" /product="oxidoreductase, short chain dehydrogenase/reductase family" /translation="MNKELINQVAIVTGGNTGIGRAVSEELAERGAKVMILARNEEKN EETKKAIVAKGGYAESYRVDVSNKEPVDDAIKSIYEKHGKIDILVCNAGNSTPLNYLT KMPMEDIEAIINTHVYGAIYCVKACGEKMKERKYGRIVIMSSLGAYHGVTGNAHYCLA KEGLVAMAQSLAKEFGRYGITVNSIQPGSINTSMSSQLLQVAKDKIVSETPVARIGEP EDIAYATAFYCSPRASFITGDTMRVDGGYIIESGMDRLILSMIDPELN" ORIGIN 1 atgattaatg aaaatacatt aatatataga ttttataata aaaatccaat gaaaaatgat 61 ttagtaaaaa tggcaatgaa aggagattca aaagcattta caagcttaat aagggaaaat 121 aaagaatacc tttataaaat ggcttatagc tacgtaaaaa atgaacagat ggcgttagat 181 atattacagg aaagtatata taaagcattt gttaatataa aaaaactaaa aagagaaaat 241 gtttttaaaa catggataac aaaaatactc ataaatgaat gtataaatac aataagaaaa 301 aatgcaaaag ttacttattt aaataatgat atattaatta aaaatgaatc tatatctatt 361 gatgaaaaac ttgatttata tgaagcgatt gaccaattga gggatggata taaaacagta 421 ataatactta agtattttaa tgatttacca ttaaaggata tttcttatat tatggatata 481 ccagaaaata cggttaaaag tcatttgaaa agagctaaaa atgatttaag taaaatttta 541 aaggaggact ttttaaatga ataagaatta tgaaaatatt agtataccaa ataatataga 601 tgattttata gacgttgggg taaagagagc tgtagattta aaaaatatta aaagaaaaaa 661 acttattaga agaaaattag ggattacagc agcttcttta attattattt taactatagg 721 tttttcaaat ccagcaatgg cagctaaaat acctattttg ggaaatgtat ttcaagctat 781 tgaaaaaaac atatattatc caagtaatta ttctgaatat tctacatcag taaatgaaac 841 atcatattcc aatggtgttg gtgtaacatt gtctgacata ttatgtgatg gacaatcatt 901 atatgtaaca tataatattg aaagtgataa accatttaaa tatacttcat ggggagatgg 961 accattaaca atgaatcagc ttataacgag tgaaaaatat aataaagtgg atttttcaca 1021 taaagaatta gataatactg gttttgcagg acttgaagga aaatttattg atgataacac 1081 ttttgtgggt atggaaaaat ataaactttc atcactaaaa acatctattc cagatgagtt 1141 tatatttcaa gttaaatatg catcatttga aacacattca ctagaggatg acgaaaaacg 1201 tgatattaaa tctggtacat gggcatttaa aatacctgta aaagttaata aagacattaa 1261 aagagttgtt aatttagata acatgggcaa tgacagtgta actttaaatt cattatcaat 1321 aacacctttt gatatgattt ttagtttaac ttataacaaa ggtaaatgga atgactatga 1381 agtttccatt tatgatgaaa atggagaaga acttaatctt tcagatagta gtatagatga 1441 agagaataaa actgaaaata taatttttaa agcgccttct aatgaaagta agagtattag 1501 gattataata aataaaccta tattagaaaa aggtgaaaca ataactgact cagaaggtca 1561 atgtgaaact tcttatgaag agcttggaaa agaattaata tttgatgaga taataaatat 1621 taaataaaca taatatgatt agatattgtt attcaatata ataaatgtaa gtatattata 1681 gaaacaaaaa tatggcatgg agaaattgca cataaaaaag gtttaaatca attaaaaaat 1741 tatctcgaaa tagaaggttt aaataaggga tatttactaa tatttaattt taatcaaaat 1801 aaagaataca ctcatagtaa atattatgta gatgataaaa ctatagttga agttagagct 1861 taaaagaaat agtagcgtca taattgaaag tgaagctact atttttgtat ttataataat 1921 gtatggagaa ccttatcgaa agtaatgaag ttataagtaa tatatctatt caaatattaa 1981 attactatgc tataattatt taaatttata atagaatttt agataggaga ttaacatatg 2041 atgaatacag atgtgtttat taatataatg agtaattata gtaatatagc attattgata 2101 agtatttttg ctagtgtgat tatagcagta ttaggagtag taccatctat atttgttact 2161 ggtgctaata ttattttctt tgatcctata aaaggttttt tcatatcatt aataggggag 2221 actataggtg cagctatatc attttggtta tatagaaaag gttttaagaa aaagatagaa 2281 aacttttcag ttaataataa atacttgaaa aaattagtaa agagtaaagg taaagagagc 2341 acttatctag tatttttaat aagattattg ccttttgtgc cttctggatt tgtaacatta 2401 ggtgcttcag taagtaatat gaatattgtt ccttttacga tagcaacttt tttagggaaa 2461 atcccttcta ttatattaga agcattggta agttacgact ttattaatat acaagataat 2521 tggattagac taataattac gttattagca ttaatattaa tcatgatttt tatgaacaaa 2581 aattataaat ctaattaggt ataactttaa aatgtatagg tgaaaacaat tatatatgat 2641 aataaatgcc tttataatca ataaaataag ttattatgaa agcattttat ttttgaaatt 2701 tttagtgata tactaaatta gttattataa agaattagca aggatgaagt ataaacatgg 2761 aaaataaaaa ttatttgtac gataattatt taaatatggc tagaaatcta aaaagtgaga 2821 gaaacttatt aaaagcttta aaattttata aaaaagcata tgcattaaat ataggtaaaa 2881 ctgatataga atttttactt gatatggcat tattatatga tgaaattggt ttaggaaaag 2941 aagcagaaga aaaatattta gaaataatta atttagatga agatgaaccc agagcttatt 3001 atggcttagg cactatacat gatgaattag gtaatttatc aaaagcaaaa gagtattaca 3061 aaatatcaat acaaaaagat tcaagttatg ataaagcata tttcttttta gccaatatat 3121 atgatgaatt aggtgataaa gaaaaggcaa ttttaaatta taaaaaaact attgaattaa 3181 ataacaagga tttatgggga tatgcgaact tagcttgcat ttttgaagaa ttagataaaa 3241 ataaagaagc gttaaaatat ataaataagg ctttagaaat aaatcctaaa cattatagaa 3301 tattatttaa taaagctgta atatcaaaca aattacataa aaaacaagaa gctatagatt 3361 attataaaaa gtctataaaa gcaaatccca catatacata tagtttttta aatttagctg 3421 tgatatacag agaagataat gatttcacaa ctgcaataaa cataataaat aaaggaatag 3481 aaataaatcc agaagaagga ttcttatatt acaatagagc ttgcttctat gttaatacaa 3541 aggaattatt aaaggcttta aaagatgtag aaaagtgtat agagctaaat gatttatttt 3601 tagattatat gaaaaaagat aaagaacttg atcctataag agatttaaag gaatacaaag 3661 aatttataaa caacattaca tagaataata tgatattatt ttatatagtg atgtaaaaat 3721 gatatgttta aagctgtgtt tatatatgta ataggtaaag tggcatttca atttttctta 3781 agttgaattg ttttcattat atttgcttat acatatataa atgctttgct aaaataaata 3841 ttaaaaaaag tggggggcga aggcttatga aagaaacttt tacagaaggt ttagagattt 3901 caatggataa tctttataaa acaggagcat ttttaacttg tggaaacgaa aataaagcta 3961 atactatgac aattagctgg ggaagtgtcg gttatatttg gagacgacct atatttatgg 4021 cactagttag agaaacaaga tatactaaag aatttttaga ttctggtgat aattatacca 4081 ttagtatacc atttgaagga agtatgaaat catcacttac tatatgtgga acaaaatcag 4141 gtagagatgt agataaagaa aaagaagcta atataaaatt tctacctagc aaaagtgtaa 4201 gtagtcctat agttcatgga tgtaataaat attatgagtg caaaataatg tttaaacaag 4261 aaatggattt agataatatt gatcctgaaa ttaaagataa attctatggt gaaggtgaat 4321 caaagcatgt attatatttt gctgaaatat tagaaagcta tactaaataa taagtattta 4381 tctatgtttt aaaatatgtt attaaacgta gtcttttatt ttcacaataa aaagctatta 4441 cttaactggg ttgtccaatc aagtaatagc tttattgtta ttttaataat tattttctat 4501 aattaatttg tgataaatta ggtgtatgct caaattcaat tggttcgttc ttgtataaga 4561 attttacacc atcaatccat tcacctttat attttacttg aagaaaagtt tctaataaac 4621 tattttcagt tatttctcca ccagtagaac cttgcaattt atcagctgtc cagtttggtt 4681 tctttaaatc agcattgcta gtaacacctt tgttagctgc gctttcatct aagtttatag 4741 tagcgatttt tttaccatct atagtttcaa tagatttaac ttctattggt aatttatcga 4801 atactttttc agaaagtgtt tttgataatt gttttaattt atcttctaaa cttaaatttt 4861 tatttaactc aattgttgaa atttctttaa tttccatact gtttgcatct tcagtgtata 4921 tagaaaatgc ttcagtttct tttttattat cattcttttc atctttagaa ctttcagtag 4981 gggtgtcttt tttaatatca ttttcattat tatctgtctt agaattttct actacagtat 5041 tgtcttctgt ctttgtatta gtagctgaat ttttagaaga acaacctact aaagctatgc 5101 ttaacattaa aacagtagct aaaatcaatt tttttaccat tatttttcct cctcatgtaa 5161 aaaaataagt aatatttaaa aatattataa taattatagc atatgtactc aggaattaaa 5221 agtttgggta tatagtgaaa ataatggtaa ataataaaaa tatattttca aaataattga 5281 caatttattt tgaatgtagt atattttgat tatcaaaatg tttgattgag aaattaaatt 5341 caaattgcta aatcaaatat ttaatgtttt atctggagga aaataaataa atggatcaat 5401 ctaaaaagat agtcaatgag atattagtag aattatttaa tgatatttta caaatagaag 5461 aacaaacaat aagagaagga gtaatttcag atctttcaat tactgaaatt catactattg 5521 aagcaattgg aatgtacaaa aaaagaacaa tgtctgaagt agctcaagat ttaaaaataa 5581 cagttgggac tttaacaact gcaataaata agcttattaa aaaaggttat gtggaaagaa 5641 gtagaataga agaagataga agagttgttt taattgcttt aactaaaaag ggaaaactag 5701 cttatagatt acatgagaaa tttcataatg atatggttag caattcaata gaaagcttaa 5761 cacaacaaga agaagaaatg ctaatttcag cattaaataa gataaatgaa tttttaaaac 5821 aaaaatataa attgaaataa ttaaggagaa aagtatgaac attaaaatag ggggagtagg 5881 gggatattta ccatcacttg ctgttacaaa tgataagatt agtgaatttg tagacactaa 5941 tgatgaatgg atagttgaaa gaactggcat aaaagaacgt agaatctcag aaggtgagaa 6001 tacttcggaa attgctgtta aggccgctaa agttgctctt gaaagagcaa aaattgattc 6061 tgaaaattta gatttaataa tagtagcaac tattactcca gatatgttta tgccatctgt 6121 agcatgttta gttcaaaaag aattaaatgc taataatgct acagcatttg atataaatgt 6181 tgcttgctca ggatttgtat ttgcattaga aactgcaaat tcattaatga aatcattaaa 6241 ttataagaat gcacttgtta taggtgcaga aacactttct aaagttatta attggaatga 6301 cagaggaact tgtatattat ttggtgatgg tggtggagca gcagtacttg taagagatga 6361 aaagaatgga ataattaatt cgtatttgaa aacagatggg aaaaaaggag actcattgac 6421 tataggtgca cttgatttta acactccttt tactaaagaa aattttatta gcaaccaata 6481 tgtagagatg aatggtagag aagtttttaa atttgcaact tcagctatta ttaaagcaat 6541 tgatggtgca ttagaaggta ctgatatttg tttagatgat ataaagtaca tagtgcctca 6601 tcaagctaat ataagaatat tagattatgt tgctaaaaaa ttaaaaatag atataaataa 6661 attttataca aatttagata aaacaggaaa tacatcttcg gcttcagtac cattagcttt 6721 aaatgaagtt tatgaaaaag gtcttttaaa tgaaggcgat aaaattcttt tagttgcttt 6781 tggtggagga ttaagttatg gagctacatt attagaatgg tagaagtcaa attattagac 6841 tatgtcttag aaaagaatat atatgtagat atctaagaca aggaaaaata ttgattaaaa 6901 ataaatatta taaggaaaat tagggaggaa ataaattatg ttatttgaag aaattagaga 6961 aattatatgt gaacaattag gtgtagaaaa ggatggaata actttagaaa ctacatttga 7021 agatttagga gcagattctt tagacttatt ccaagttgta attgaattag aagaaaaatt 7081 tgatattcaa atagaagaag ttgaaaagtt aaagagttta aaagatgcag ttgaatatgt 7141 aaagaataaa actaataact aaaatttaaa atattaacat ccaagttaag tcgggtatgc 7201 aaataagtat tcccgattta atttatataa tgtaagtatt tttataagaa cactattagg 7261 aggaattatt tgtggcaaat aatagagtat gtgatctact tggtataaag tatccgatat 7321 ttcaaggagg tatggcttgg atagcagatg catctttagc agcagcagta agtgaagctg 7381 gtggaattgg aataataaca ggagcagcac caacagaatg ggtaagaaat caaatcagag 7441 aagctaaaaa attaacagat aaaccttttg gtgtaaacat aatgctaatg tctgaaaatg 7501 cagaagaaat agcagaacta gtatgtgaag aaaatgtagc tattgttact actggagcag 7561 gaagtccagg taagtacatg gaaagatgga aagcaaacaa tataaaagtt ataccagttg 7621 ttgcatcagt agctcttgct aagagaatgc aaaaagcagg agcagatgca attatagcag 7681 aaggtacaga atcaggtggt catgtaggtc agcttactac aatggcgtta attccacaag 7741 ttgtagatgc agtaagtata ccagttatag cagcaggtgg aataggtgat ggaagaggag 7801 ttgcagcttc ttttatgtta ggagcagaag gggttcaggt tggaactaga tttttagttg 7861 caagagaatg tacagttaat caaaattata aagataagat attaaaagct aaagatatag 7921 atacagaagt tacaggtaga tctacaggtc atccagtaag agtgcttaaa aacaagctat 7981 caagaatgta taacaagtta gaaaaagaag gtataagttc agaaaaatta gaagaattag 8041 gagcaggtac tttaaagaaa gcagctgtag atggggatat tgaaaatggc tctataatgt 8101 ctggacaaat tgcaggctta attaacaaag aacaaacatc aaaggaaatt attgaagaga 8161 tgtttgaaga agcacagcaa aggtttatgt tatttggagg aaatcatgaa tagtgaaaat 8221 attgcatttc tttttccagg tcaaggtgct caatatgttg gaatggcaaa ggaattctat 8281 gataaaatac cggaatgtaa agaaatagtt gataaagcag aaaaaatatt agatatgcca 8341 ataaaagata tgctatttaa tggtccagaa gaagtattaa tgcaaacaga aaatgctcaa 8401 cctacaatag tagtggcctc tttagtagca ttaaaagctt tggaagtaaa taatattgaa 8461 gcaaagtaca ctgccggatt aagtcttggt gaatatgctt ccttaatttt ttcaggagca 8521 ctttctttag aagaaggatt acttcttgtg aaagagagag gtaggataat gggaagctct 8581 cttccaaaag gtcttggaaa gatggcagct attttaaaac ttgataatga aaaattagaa 8641 gagctattaa atagagcaag tgaatttggt gttgtagaag cagcaaatta taattgtcca 8701 aagcaagtgg caatagctgg tgaaaataag gctattgatg aagcagtcaa aatagcaagg 8761 gaacttggcg gcttaggaat acctttaaaa gtaagtggac cttttcatag ttcgttatta 8821 aagagtgcaa gtgaagaatt ttataaaaca ttaaaagata caaacataaa ttcattaaat 8881 aaagttactt attctaatgt gaaagggtta ccttatgatg aaaaagatga tattaaagag 8941 ttgttaaaaa agcatataag atcatctgta ctttttgata agactattga aaatatgata 9001 aaaagtggag ttaatacatt tattgaagtt ggaccaggta aggttcttag tggatttgta 9061 aagaagatta atagaactgt taaagtatta aatatagaag atatggaatc attaaataaa 9121 ttattattaa cattagaaga aatttagctt aaggaggatt ttaattaatg ctaaagggaa 9181 aatgtgcaat tattacaggt gcagcaagag gaataggtaa agcaattgct ttaaaattag 9241 cttctcttgg tgcaaacata gtattaaatt atagaagtag tgaagaagag gcaaaaatag 9301 tagcttctga aatagaaaaa atgggtgtag aagttttaac tgttaaaggt gatataagca 9361 aattagaaga cgtagaaaac attataagtg aagcaaagaa caaattcgga attatagata 9421 ttattgtaaa taatgctgga ataacaaaag atacccttat tcttagaatg aaagaaaaag 9481 attttgatga tgttatagat gtaaatttaa aaggagtttt taattgttta aaatcaatta 9541 ctcctgttat ggttaagcaa agacatggaa agataataaa tatttcatca gtagttggtg 9601 ttgcaggaaa tgcaggtcaa gtaaactatg cagcttctaa agcaggggtt ataggtatga 9661 ctaaatcttt agcaaaagag cttggagcaa gaggaattaa tgtaaatgca gttgctccag 9721 gattcattga aacagatatg acaagtgttt taggtgaaaa ggttaaagaa gaagctaaaa 9781 agaatatacc attaaagaga tttggaactc cagaagatgt agcaggtgtt gtagcttttc 9841 ttgcaagtga aaattcaaac tatgttacag gtcaagttat aaatattgat ggcggaatgg 9901 taatgtaatg gaggagtata gagttatgga aagaagagtt gttgttacag gaatgggagc 9961 attaactcca ataggaaatg atgtagatac attttggaaa aacaataaag agggtaaatt 10021 aggaatagat tttattaagt taatagataa tgaattaatt aatgtgaaga ttgctggaga 10081 acttaaggat tttaatccag aagaagtttt aggtaaaaaa gaatgtaaaa gactagatag 10141 attttctcaa atggcattaa ttgcagctga tgaggcaatt aaaagttcta aaatagattt 10201 agaaaaagta gataaaaaaa gatttggtgt tatggtaggt tcaggaatag gtggatttgc 10261 aactatagaa actgaatgta ctaaattagt tacaggtaaa tcaaagagaa tgtcaccatt 10321 ctttgttcca atggcaatta tcaatttagc tgctggaaat atatctattc aatatggact 10381 tgaaggttct tgtacatcag tagttacagc gtgtgcaaca ggtacaaata atattggaga 10441 tgcatttaga tctataaaac atggatatgc tgattttatg ttagcaggtg gtgcagaagc 10501 tccaataaca agaattggtg ttgaaggatt ccatagtatg aaagcactta attcttcaaa 10561 tgatcctatt aaagcttcaa ctccttttga taagaataga agtggctttg taatgggtga 10621 aggtgcagga atattattac ttgaatcttt agattcagct ttagaaagag gagcaaatat 10681 attaggcgaa attgtaggat atggttcaac ttgtgatgca taccacatta catccccaca 10741 tccagaaggt tttggagcag cagaagctat gagtcaagca attaatgaag ctaaaataga 10801 taaaactgaa gtttcatata taaatgctca tggaacttca actcaattaa atgataaatt 10861 tgaaactgca gcaattaaaa aagtttttgg agaagatgca tataacattc caatatcttc 10921 tacaaaatca atgacaggac acttattagg tgcagcagga gctattgaat ctatagtgtg 10981 tttaaaagca ctagaagaag gatttgtacc gcctacaata ggttatgaaa ctaaagatga 11041 agaactagat ttagattatg ttccgaataa gggaagaaaa aaagaactta agtatgcttt 11101 aacaaattct ttaggatttg gtggacataa tgcaacttta ttatttaaga gatgggaaga 11161 aaaataggag gcaattttat ggattttcaa aatattaaag aacttataaa tattataaat 11221 tcttcggatt tagcttattt tgaattaaaa tcaaatgatg gctatgtaaa aatggataag 11281 tctttaacta gaagcttaaa tagaaatgta gaagaagaag agactacaaa aaaggaaagc 11341 aacattcata caaaagagga acctatacaa gaaaagaata atttcccaga aaataaatcg 11401 agtaatatgc aagaagaagc tgtagaagaa gacacagtaa taattacatc accaatggtt 11461 ggaacatttt attcatcacc atctcctgag agcgagtcat ttgctaaaga aggagattat 11521 gtgaaaaagg gaaaagtaat ttgtataatt gaagcaatga agttaatgaa tgaaattgaa 11581 agcaattata atgggaaaat agtaaagtgt tttgcaaaag atggagatat ggttgagttt 11641 ggacaaaaac tctttgaaat taaggaggat taatatttat gttaaagata gaagaaataa 11701 aagaaatttt acctcataga tatccatttt tacttataga tagagtaact gaaatggata 11761 tagaagaaaa actatttgtt aagggatata agaatgtctc tgcaaatgaa caattttttc 11821 aaggccatta tccacaagag ccaattatgc caggagtttt acaaatagaa gctttggcac 11881 aagcaggagc tgtggctatt ttatcaatgg aaaagtttaa aggaaaaact ccattgtttg 11941 ctggaactaa taaggttaga tttaaggcaa aggttgtacc aggagataga ttagatttgt 12001 attgtgaaat agtaaaactt aaggggccaa taggaatagg taaaggaata gcttcagttg 12061 atggtaaaac agtttgtgaa gctgaaatac tttttgctat aggataggtg aaggatatgt 12121 ttaaaaaggt acttattgca aatagaggag aaattgcagt tagaataatt agagcttgta 12181 gagaaatggg aatacttaca gttgctgttt actcagaaat tgataaagaa gcacttcata 12241 ctcaattagc tgatgaagcg atttgtatag gtccagcaat agctaaagat agttatttaa 12301 atattgctaa tattttaagt gcatgtgtac ttagtggggc tgatgcaata catcctggat 12361 ttggattttt atctgaaaat gctaagttcg caaagatgtg taaagaatgt aatataaaat 12421 ttataggtcc agattatgaa tcaatcgaac ttatgggtga taaggcaaga gctagagaaa 12481 taatgaaaaa agcaaatgtt cctgttgtac ctggatatga aggggaaata gagaatgaaa 12541 cacatgcttt aggtattgct aaaaaaatag gttatcctat aatgataaaa gcagctgctg 12601 gtggtggtgg aaaaggaata agaattgcca gaagtgatga agaattttta aagggatata 12661 aaactgctaa atctgaatct aaagcttgct ttggtgatga tagattatat atagaaaaat 12721 ttattgaaaa acctagacat attgaatttc aaattttagc tgatgagtat ggaaatgttg 12781 ttcatttagg agaaagagaa tgctctcttc aaagaaagaa tcaaaaagtt ttagaagagg 12841 caccatctaa tatattaaat gaagaaataa gaaatacgat gggagaaata gctaagaaag 12901 cagccaaggc tgttaattat aaaaatgcag gaactataga atttttattt gataaggata 12961 ataactttta ctttatggaa atgaacacaa gaatacaagt agagcatcca attacagaaa 13021 tgattacagg tgttgattta gttaaagagc aattaaagat agcatcagga gaaaagttat 13081 tattaaaaca agaagatata aagatatctg gtcatgcaat tgaatgtaga attaatgcag 13141 aagatccaga aaatgatttt agaccatgtc ctggagaagt atcagagtta tgtgttccag 13201 gtggaatggg agttagagta gactcggcta tatattgtgg atataatata ccttattttt 13261 atgattctat gattgcaaag cttataactt ttggaaaaac tagagaagaa gctattgtaa 13321 aaatgaaaag agccttaggt gaatttgcag taggtggagt aaaaactaat attgattttc 13381 aattttctat acttgaaatg caagagtttt tagatggaaa ttatgacacg tcatttttat 13441 cagaaaagat ggtgaagaat aatgcttaag gatttattta aaaaaagtaa atatgcaacg 13501 gttaatccat cagcatataa aagtaaagta gtagaagaga agcctaatat tccatctgga 13561 atgtggacta aatgtagtaa ttgtaatagc atgatttact atgaggattt agaaaacaac 13621 aaatatgtat gtactaagtg caatcaacac tttaggatat cacctaagga aagaataaag 13681 cagatatttg ataaagatac ttttaaagaa atgtggaaat cattaaaaac aaataatcct 13741 attgactttg aagactatga agagaaaata aataaatctc aaagcaatac tggaagtaaa 13801 gaggcagtag ttactggagt aggtagaata aatgatttgg aagtagcttg tgcaataatg 13861 gatagcttct ttatgatggg aagcatgggg actgttgttg gtgaaaaaat tactagaata 13921 gtagaatatg ctacagaaaa taatcttcct attttgattt ttacagcttc aggtggagca 13981 agaatgcaag aaggtatttt ttcgcttatg caaatggcaa aaataagtgc tgctcttgca 14041 aggcatgatg aaaaagggct attatatata acaatattaa ctgatccaac tactggagga 14101 gtaacagcaa gttttgctat ggaaggcgat attatattaa gcgagcccaa tactttagtt 14161 ggatttgcag gtagaagagt tatagaaaat acaataaatg aaactcttcc agaatcattt 14221 caaaaatcag aatttcttct tgaaaaagga ttcatagata gcattgtaga aagaaaaaat 14281 atgagagctt atttatataa gattcttatt ttacatggag tgaataagta tgaataaaga 14341 gtttataaaa tcaatagtag tatcaagccc ttgggaaaag gttgaaatag ctaggcataa 14401 agatagacct acaggaaaat attatataga taatattttt aaagatttta tagaattcca 14461 tggagataga ttatttggtg atgataaagc tataattggt ggaatagcat catttgaaga 14521 tattagcgtt acagtaatag caataacaaa gggagctaat acaaatgaaa atatagaaag 14581 aaattttgga atgccaaatc cagagggata tagaaaagca ttaagactta tgaaacaagc 14641 agaaaagttt aatagacctg ttatttgttt catagatact ccaggagcct tttgtggagt 14701 tggagcagaa gaaagaggtc aaggtagtgc tattgcaaat aatttattcg aacttagtag 14761 attgaaaact cctataattt ctatagttat tggagaaggg ggaagtggag gagctttagc 14821 attaacagta gcagataaaa tacttatgtt agagaatgca gtttattcaa tactttcacc 14881 agaagggttt gcatctatat tatggaaaga tagcaaaaga gtaaaagaag cagcaaatgt 14941 tatgaaaata acagcacaag atttaaatga gtttgggatt atagatacgg taataaaaga 15001 accaagaggt ggagcacata aaaatccaca aaaacaagta acgctcataa aaaaagaaat 15061 tatgaatgca atgaatgaaa tgaaaaacat tgaaacaaat caaatgataa atgaacgtta 15121 tgataaattt agaaaaatag gaactttaga gtaatttatg atatatagtg atttttaata 15181 taagctcaaa agattgcaat cttttgagct tttttgatta tacatatttt aatatatttt 15241 ttaagctgag aaagttaaat atgagtcatt gacaatatat actatttaga aataatataa 15301 tattaaagtt aaattttttt aagaggatga tgattatgga tgttgatctg aaaagtaagg 15361 tagatatatt taaagattat tataaagata taagcagaac attaatattt gataataata 15421 aagttaaagt attagaaagc ttactctata taataaataa gaatgaagta gataaaagtg 15481 aattaaaaag tattagaaga tacataagta aaagtgaact aaatagtgta tttaatggaa 15541 atgttaaaaa agcaatgtca gcttttttat gttcaaattt tcaatatgaa aatatagtta 15601 aagatacaat tataatatat gataatttag ttaataaagg ttttaaaaag gacaagtact 15661 tagagctttt atcgtttatt attactttaa gatttcataa aaaagaatat gataatatat 15721 taaataatat gttaaaggca aaaactgaaa taaaaagcaa aatacaagga atagatgaat 15781 ttaaattaat gaatatatgt tttataaatt tatcaatgtt ttctagagag tatgatcaat 15841 tagttagaaa gtatttaaaa atatatttta aattacttag gaaaaacttt gataaaaaag 15901 aagctttttt tattgtgctt gccatgataa atttagatga gatagattat actagttata 15961 taaataatgt tatagagata aagtcacatt taaacaatac taaaatggaa ataaaagaag 16021 agcattattt attgttaggt ttatcttcat taataattga aaataatagt gaattcgtta 16081 ataatattag tgaagtatat aatgaattat gtgaaaaaag agctataaaa aaagatcagc 16141 ttgtattagt atctattaca ttagtattat atgaatacat tgaaattatt tcagaagatg 16201 atgaggattt gtttactgaa aatgaaataa acatagttaa gaagttaata gaatatctaa 16261 ctgtattgtt aatataataa aataaattaa tattaaagga gaagaatctt atggagagta 16321 atagcacaag ggttgctaaa atagcaacta aaatggctat atgtgataga tttgaggaag 16381 atgatttaaa aaagatatat aacaaagaag gtataaaagt tacagcagta aatataggtg 16441 gaaatattaa ttcatcaata tctaagatat tagaaagtgc actagttgca tcaaaaagaa 16501 atggtctcat aagagaagaa catttacatg aaggtgcagt aataggagcg actagagatg 16561 ctataataca agtttctaat agagctaatg cacaaaatgt aggtggaaag attggaatcg 16621 ctagaggtgg agaacacatt tctgtatgta tattcttaag tattggattg cttcatttag 16681 atgaagttgt tataggtata ggacacaggg ctataccaga ataaaaatat tatgatatgt 16741 ttttgtgaat ttttgatata cgtataagca aaatggatgg agtaattgaa attatgaata 16801 ctaaaataat ataattaaat agagagaaat agtaaataga tatttactta tattgttagg 16861 atagatatta agataagaag agttatttta atatctatcc tttttattat agagattaaa 16921 ataagtgtgt tttgaaaaat aaatttaaag tttaaaggac aagtgcataa cagctgattt 16981 aattttagga tagtttttta taatatggat taaaaaatat tatcgaaaat ttgtataatc 17041 attacttttt tgttatatta atgaatgtgg aaaataatag ttaaattaat aataactata 17101 aatttttata ataatataaa tacaagaaaa ggaagatggg gattatgaac aaagaattaa 17161 ttaatcaagt tgcgattgtt acaggaggga acactggaat aggtagagct gtcagtgaag 17221 aactagctga aagaggcgct aaagttatga ttttagctag aaatgaagaa aaaaacgaag 17281 aaacaaagaa agctattgtg gcaaaagggg gatatgctga atcatataga gtagatgtat 17341 ctaataaaga gcctgtggat gatgctatca aaagtattta tgagaaacat ggtaaaatag 17401 atatattagt ttgcaatgca ggaaactcaa cacctttaaa ttatcttact aaaatgccaa 17461 tggaagatat agaagctatt ataaatacac acgtttatgg agctatctat tgtgtaaagg 17521 catgcggaga aaaaatgaaa gaaaggaaat atggaagaat tgtaataatg tcatcacttg 17581 gagcttatca tggggttacg ggaaatgcac attattgttt agctaaagaa ggcttggttg 17641 ctatggcaca aagcttagct aaagaatttg gacgatatgg aataacagtt aattcaattc 17701 aaccaggatc tattaatact tctatgagtt ctcaattatt acaagtagct aaggataaga 17761 ttgtgagtga aacaccagtt gcaagaatag gggaaccaga agatattgct tatgctacag 17821 cattttattg tagcccaaga gcaagtttta taacaggaga tacaatgcga gttgatggtg 17881 gatacattat tgaaagtgga atggatagat taatattaag catgattgat cctgaattaa 17941 attaa //