LOCUS NC_017243:10 18030 bp DNA linear BCT 04-SEP-2013 DEFINITION Brachyspira intermedia PWS/A chromosome, complete genome. ACCESSION NC_017243 SOURCE Brachyspira intermedia PWS/A chromosome, complete genome. COMMENT locus start: 685783; locus end: 703812 COMMENT n1_4mer:GRV/n1_4mer:RV = 1.986749; n0_4mer:D = 13.633876; n0_4mer:PS = 23.510781 FEATURES Location/Qualifiers source 1..18030 /organism="Brachyspira intermedia PWS/A chromosome, complete genome." gene complement(1..876) /gene="ybfH" /locus_tag="ybfH" /db_xref="685783..686658" CDS complement(1..876) /gene="ybfH" /product="hypothetical protein" /translation="MWGITFISTKILLRDFSPIEILFSRFLLGFALLFIIYPKNNKIY SKKEEMLFAVTGLSGITLYYLFENIALNYSLASNVGILVAIGPLFTGIFASIFLKEKL KLNFFVGFIFAIIGIAVITFNGKFILKINPIGDIFAILAAVMWGIYSVLVKKIADFGH NSVLITKKTFMYGIIFMIPVMLFMGFNVNINDYLKPINLINFLFLSFIACTLCFITWA YATKILGAVKTNTYIYFIPIITLITSKIVLDENITLFAIIGIIFILLGVIISQANISF KKMNKINTRKIMNNQ" gene complement(944..1090) /gene="" /locus_tag="" /db_xref="686726..686872" CDS complement(944..1090) /gene="" /product="hypothetical protein" /translation="MIKKIYCLLVLIMLLLSCADKKEADNKNSIKVMEDDEYIKNVIK DSSK" gene complement(1116..1337) /gene="" /locus_tag="" /db_xref="686898..687119" CDS complement(1116..1337) /gene="" /product="hypothetical protein" /translation="LILSYGFLEQENTNNNDTEIIKYNKIFFIFIVPLNYPNIMLINI ILYLNILSNYFIDKVIIFYFFNYYTVYII" gene 1339..1644 /gene="" /locus_tag="" /db_xref="687121..687426" CDS 1339..1644 /gene="" /product="rhodanese domain-containing protein" /translation="LKKALDLMSKSTNLVLLDVRTPEEYMGGSAPNSINIDVLNTDFK SKIDLLDKNKEYIVYCRSGNRSSIASSIMATNGFLHVYNLQNISYVDFVNAILTNRK" gene complement(1719..2147) /gene="" /locus_tag="" /db_xref="687501..687929" CDS complement(1719..2147) /gene="" /product="hypothetical protein" /translation="LINLEKEEILYKSIKKGVIYGKNIIKNSIKEYYINNDKNIILII EKDINASIYNELYNILNRLLENHAKNIIIHFRINIKYIISNAIYLMLEVQKVLEFRGG NLILTGLNEYSKWSLKSLEAHKKVKIHDDIQNAIKEIKAS" gene complement(2113..2349) /gene="" /locus_tag="" /db_xref="687895..688131" CDS complement(2113..2349) /gene="" /product="hypothetical protein" /translation="MKINNTIYNMKKSRSIYSNRSAVIQNDEEIVLLLGNEMRKENTV DVDTTVYLSLPSFLRLYDTLKEKVDKFREGGNLI" gene 2723..3388 /gene="" /locus_tag="" /db_xref="688505..689170" CDS 2723..3388 /gene="" /product="hypothetical protein" /translation="MTPEKVVSMISNRFSTIKGFSANFVERNGERISYGNVITKNPNL FRMEYSSGGNGQSIYCNGETLWIIFPKNNVVAEQKLNYGDSGAIYTPEGIMRLTTKFN IDFYNERELVSLNSFKDSELGIAKYNSSYAANDNRRAYHMLLTSKQASVDRTGFTKIH LWVDNTGMIIRILGITTTNVPVEYLFTSIRYNPKYEDNNKDFEPEISENMQVLKDGLV PNN" gene 3403..4785 /gene="" /locus_tag="" /db_xref="689185..690567" CDS 3403..4785 /gene="" /product="hypothetical protein" /translation="METIGQILKNAREKKGLTIEELEATTHIVARFIKALENEEFDVL PGEIYVKGFIKNLSDKLSLDADMVLERYNLQKNGIKSEQDLLKSGKAIKNIKKNKEPV KEVKDNKDINDGKDNKEQEVNKEPVKDTNTKESLKDVINTEKHEEKTIKETNKKNKNT NTNNRAEADLLFMARRDLYKLRNKNKSIIPTVIIILAIIVVIAVIVFNWKNITGFIFP SNNKRQNRNEVEISKNIVDSKAKQSVKSGDIIYFKPLGISATIKFNSIGNVLHINING QDLSFSKSSPIILDLNGNGINDFKISVIEVYDNSATVEMEKLEENQMVNTGYTNDIES AAMNTTNYNNTVSTNLLVINGETYIEQDTEKVDIRIEITAKHFVYIRYFIDSNRPATT NLLSGKTLYLEAKDVVMLTIGNAGEVVVKVNGKIINVGAAGETVNKTIKWVKNLNDST RYNLIMSDTK" gene 4855..6177 /gene="" /locus_tag="" /db_xref="690637..691959" CDS 4855..6177 /gene="" /product="MiaB, 2-methylthioadenine synthetase" /translation="LQNIYLHSLGCEKNTVDGEHILAILQKKGFNITNNPEDADIIVI NTCAFIEDSKKESIDAIFDHSMYKKYGKCKRLIVSGCMSERYKENFLEMFKEVDSAIG IYDLENILKAVEEDGFHDAKENTEYKEYGDRVNTGTKYSVYIRISDGCHANCSFCAIP GIRGDHRSRKIEDIVKEAENYAKNGAKEINLIAQETTFYGYDIYNKLALPDLLKALSK VEGIQWIRVLYQNPVVLNDEIIESFFNTDKVVPYFDIPLQHVDKDILKDMNRGTRSYK FYREMIDKIRSYNNDAVIRTSLIVGFPGETPESFKKLIKFVRNAKLDRVGVFTYSEEE NTKALLIDKPKLSRNRKLMLREKLMRAAIEVSEKRLERFIGRTIDVLIEKKEDENKFI GRSIYDAPEVDGYVEVYNENTNNNINIGDIVQVTVTHNTKYDLIGNVI" gene 6195..8033 /gene="" /locus_tag="" /db_xref="691977..693815" CDS 6195..8033 /gene="" /product="membrane-associated ATP-dependent Clp protease ATP-binding subunit" /translation="MKKKKQENEPSSIEKEEKKSSYANREDAFRAYFEIVTKIVYTLN LISDRNDMTRRLSSNIDRALGYDDEMMSNNEKIANNSNEVEILINSLKTDVRKISTFK KYNFNIERFISDKNLDLDERFILYSVLVYTIYGDSMSAISVRRILELITLNNDIYINK YHYFDNNSKLISSGIFNLSHEPYPSSGILEITNTLITFISSKVIFAFLEKDNYNVIES IISSGEAIKKISLENKIGGKRKSSESDILTPKEIVAQLDKTVIGQDEAKKALAVHAYL HCLRINGNKDIPFRSNILMIGPTGVGKTYLVKTLADILGLPFARADVTTLTETGYVGD DVEVVLYNLYRKANGDLEKAQHGIVFLDEVDKIAKADAHQSTTGNPSDKAVQEALLSM MNGEDIRVPEFGDRRMMHSSDGILMNTKNILFIFGGAFVGLDDIIKMRLKGESSLGFG SNAVINKLQKNRILSQVDVKDIEKYGMIPEFIGRIPIIVTLNDLTKENLKDILTKTSE SPIIKYTEFFKSIGKKLVVTAEAINFIVDKASSMNMGARSLKSIVETAMVNILFNLDG VKGNTLTLTKYDIENAFSEKEFVNSGNEDKKTDNSNKNFSKESFMA" gene 8030..8689 /gene="" /locus_tag="" /db_xref="693812..694471" CDS 8030..8689 /gene="" /product="RpoE, DNA-directed RNA polymerase specialized sigma subunit, sigma24" /translation="LILLYVCLFVINNVYINYLRTGNILEFVEMIVTKEKELLKDYEL IEAHKKGDSEALDILLTRYKNYIFNLSYRFMHNYEDAMDLTQDVLIRVYKAIDRYEER NYFKGWLYRIISNTAINMYSKAYRRESPYLEKLEIVDDKRYNTEKEYERIYLQEKIYN ASTKLKGKQRDVFILRYYENYSYKEISDILNISSDSAKSNYSYALKKMKSLLEKERTL L" gene 8758..9015 /gene="" /locus_tag="" /db_xref="694540..694797" CDS 8758..9015 /gene="" /product="hypothetical protein" /translation="LPDREWQYMLQRAKMESIKENEISPLYAFMTSARLSIALSTAIV LVLALSLFYGNPNKEDSHNTNTASSSKTNINVVNVANSSFI" gene 9184..11670 /gene="hlyB" /locus_tag="hlyB" /db_xref="694966..697452" CDS 9184..11670 /gene="hlyB" /product="hemolysin B ATP-binding subunit" /translation="MFQFHLTSKAKKVIELYAQEEAKRLNHDMVTPEHILLGLLHESE ALATRVLLRLKIDLDRLKLELESAMVKSSTTKVFGTLPTAPRVQKLISRSAEEARALS HNYIGTEHLLLGLLREESGTAYNVLTSMGLELTILRQEILKMLGVAGSNISSMEQTTQ EDNVKKVKTPTLDQFARDLTKMARDKALDRVIGRENEVMRVVQILSRRKKNNPILLGE PGVGKTAIVEGLAEKIVAADVPDILLKKRVLTLDLSSVVAGTKYRGEFEERIKNIVLE IKKASNIIIFIDELHTLIGAGGAEGALDAANMLKPALSRGEIQCIGATTINEYKKYIE KDGALVRRFQPINVEEPSIEDTIEILNGIKGKYEEHHKVKYTDEAVNAAAVLSKRYIF ERHLPDKAIDLIDEAGSRARLLNMTRPQEFKDLDKKIEELNQQKKNAVDNQNFEDAAK IRDEISSIQEELAKKEEKWREEREKIETFIEEDDIRHVISEITNIPIKRLLNSESKRL IGMEEELHQKVVGQKEAISSISKAIRRSRAGLKTSKRPLGSFIFLGPTGVGKTALAKV LSEFMFGDSDALIRIDMSEFMEKFAVSRLIGAPPGYVGYEEGGGLTEKVRRKPYSLIL FDEIEKAHPDVTNILLQVLEEGQLTDNFGRKVDFSNTIIIITSNLGARDIIKGSSLGF NAVGSEKDANDIKNFALEELKQNFNPEFLNRIDDIIVFHTLSKEDLKDIINIMLKELN EAIKERNIVINLSEEAKNYIIDKGFDKKYGARSLRRAIQKEIEDYVSTEILFGNIEDG DTINVDANDGSLIFSYEKSTKTENKELSKS" gene complement(11764..12327) /gene="" /locus_tag="" /db_xref="697546..698109" CDS complement(11764..12327) /gene="" /product="hypothetical protein" /translation="MRIYICFIISITFIISCQTNQVYNIKNDDDKSHFVKINRIEYLK PNIKYNINGTLYSTDKQSRIYKVIRKDNLILSNAKRNNYAQRKVVELYGIKNHDQGGH IIGRQFGGSPNIDNIIPINKKLNIGEMKNTEMEWRESIIKGYEINDIIIDISYIYTNT RPYIITIDYNIEKDFTNYRIKKVFTND" gene complement(12738..14537) /gene="" /locus_tag="" /db_xref="698520..700319" CDS complement(12738..14537) /gene="" /product="hypothetical protein" /translation="MMKIKKIMATIALLSFTWSILPAQTYEDAARITQEADDLQNQGQ YQQSYDKSQEASLSIDKAQMALFYRLMNARINKTKNDANNAITEINQMGAATDNQFKT QYEEAVKYFEEGNANIGSIPGPDTVAQTDEEFNTASNTFNTVLEYYNNALSSANSVKE GYLGRERDTASKAIADARGKYNAALGKSIKAGDNNDKTVSGALTKADEALKADNFASV QQNVAAALAGITKAEADAKAAAERAAAAAKAKAEAEAKAKAAAEAKAKAAAEAKAKAE AEAKAKAAAEAKKKAEEEARKKAEQEALAKEKADAIAKAKQDIANAQQKYDGLVNDNT ITRGDEADQNISTLLADANNVVETDPAAASQKALEASKAMDDLVTERDNTIAREENQR QLDDLKARYQQLVDEGYIIPDSEEDQNLSQILKDAEDALANNDNTLAREKLEEANRTM NAIYEMGPKRPVDGDLVDTDNNNNETGQIIDATTGQTVNTEGKVTVLPMYYTVVQRTP LTDALWRIAGYSFIYNDPIQWYRLYQANRNVLRDPNNPDLILPGQVLTIPSMNGEERA GTYDPNMEYITYDEAMILRNQQQNNAGTTQTNN" gene complement(14554..15162) /gene="" /locus_tag="" /db_xref="700336..700944" CDS complement(14554..15162) /gene="" /product="hypothetical protein" /translation="LQIGFITLIAFMLISCGGLPTKEYERVTALRDTVVNKYPEIQTF AQEDFDIAEKGYAEADIIMKEENKDEAEKAKELLLAAETNYNLVLDKGLPPYSEILKK QTDESATNAIDIKAGVMYADEFAKADTVYQEANAMYTAETKDYRVMVDKLYQAKVAYT DLYNKTKEQFDKSDEALKLVKERLAQLEKMMQEIEAFEQEQN" gene complement(15450..16808) /gene="" /locus_tag="" /db_xref="701232..702590" CDS complement(15450..16808) /gene="" /product="glutamate synthase (DPH)" /translation="MPARSPEERRKDFKEVPLGYTEEQAYQESLRCLDCKVPHCMEGC PAKVKIPEFIGLIAEKKFLEAAKKIKETNALPAACGRVCPQEEQCEQRCVVGKKFEPV AIGKLEMFVADYERKHAQHEDLKVEKNGKKVCIIGAGPAGLACAGDLIKLGYDVTVLE ALHTIGGVLMYGIPEFRLPKELVAHEVENLKKDGVNFRINEVAGISLDFNELRKEYDA IFLGTGAGLPAFLNVPGEHLCGVYSANEYLTRVNLMGAYKFPEVDTPIIKHKRVAVLG GGNVAMDACRTAVRLGAEKVYIIYRRTEKELPARLEEIHHAMEEGVDFRFLRAPLEIL GDENDNVIGVRTQVMELGEADADGRRKPVAVEGQTEDIEVDAVIVAIGTTPNPLIARK VPELETTKKGTYVINEETGATSMEGVFAGGDAARGAATVILAIGDGKRAAAGIDKYLS NK" gene complement(17116..18030) /gene="" /locus_tag="" /db_xref="702898..703812" CDS complement(17116..18030) /gene="" /product="N-acetylneuraminate lyase" /translation="MRNLDKYKGIIPAFYACYDEKGEISPERVKKFTQHLIDKGVNGL YVGGSSGECIYHSKEERKLVLENVMEVAKGKITIIAHVGCNNTADSAELAAHAEKLGV DAIASIPPIYFHLPDYAIADYWNDISAAAPNTDFIIYNIPQLAGVALNVNLYKKMREN PRVIGVKNSSMPVQDIQMFKDVGGDDSIIFNGPDEQFVAGRLIGADAGIGGTYAVMPE LFLAANDAVNKCQFDKARDIQYKIDRIIYAMCECHGNLYAVMKAILKLKGLELGGVRK PLSNIIDDDKAKIEKCAKMIDDAINSIK" ORIGIN 1 tcattgatta ttcattatct ttcttgtatt tattttattc atctttttaa acgaaatatt 61 tgcctgcgat attatcacac caagcaatat aaaaattatt cctataatag caaatagagt 121 aatattttca tctaaaacta tttttgaagt tattaaagtt attattggta tgaagtatat 181 atatgtgtta gttttcacag ctcctaatat ttttgttgca taagcccaag ttataaagca 241 taaagtacat gctataaaag ataaaaataa aaaattaatt aaatttatag gctttaaata 301 atcatttata tttacattaa atcccataaa taacataacg ggtatcataa atattattcc 361 atacataaaa gtttttttag ttattaaaac tgaattatgc ccaaaatctg caattttttt 421 tactaataca gaatatattc cccacataac agcagccaat atagcgaata tatctcctat 481 aggatttatc tttaaaataa actttccatt aaatgttatt acagctattc caattattgc 541 aaatatgaat cctacaaaaa aattaagttt tagtttttct tttaaaaata ttgaagcgaa 601 tattcctgta aataatggcc ctatagcaac taaaatacca acatttgatg ctaatgaata 661 attcaatgct atattttcaa atagataata aagtgttatt ccgcttaatc ctgtaacagc 721 aaataacatt tcttctttct ttgaatatat tttattattt ttaggatata ttataaataa 781 taaagcaaat cctaataaga atctgctgaa aagtatctct ataggtgaga agtctcttaa 841 taatattttt gttgaaataa aagtgatacc ccacatcaat atactgaaaa atgtgcatag 901 gtatgcttta atattttgat ttataaacat ggtgtttttc caattatttg gaagaatcct 961 ttataacatt ttttatatat tcatcatctt ccataacttt tatagaattt ttattatctg 1021 cttctttttt atcagcgcaa gataataata acataattaa aactaataaa caatagattt 1081 tttttatcat tacattatcc gcaaaacaaa ttatactata ttatataaac agtataataa 1141 ttaaaaaaat aaaaaataat tactttatca ataaaatagt ttgacaaaat gtttaaatat 1201 aatattatat ttattaacat aatattcgga taatttaaag gaactatgaa tatgaaaaaa 1261 attttattat attttattat ttcagtatca ttattatttg tattttcctg ctccaaaaaa 1321 ccatatgata atatcaattt gaaaaaagca ttggatttaa tgagtaaatc aactaattta 1381 gttttattag atgttagaac accagaggaa tatatgggag gaagtgctcc taattctatt 1441 aatattgatg ttcttaatac tgatttcaaa agtaaaatag atcttttaga taaaaataaa 1501 gaatatatag tgtactgcag aagcggaaac agatctagta tagcttcaag tataatggct 1561 actaatggat ttttacatgt ttataatttg cagaatataa gctatgttga ttttgtaaat 1621 gctatattaa caaatagaaa gtaatttctt taatatatta atttataagt aagcaaggat 1681 attaaaaatc cctgcttatt ttttattata ttatgatttt atgatgcttt tatttctttt 1741 atagcattct gtatatcatc atgtattttt acttttttat gtgcttctaa tgattttaaa 1801 gaccattttg aatattcatt taaacctgtc aatattagat ttcctcctct gaattctaat 1861 accttttgaa cttcaagcat taaatatatg gcatttgata ttatatattt aatgttgatt 1921 ctgaaatgta taatgatatt tttagcatga ttttccaaaa gcctgttcaa tatattataa 1981 agttcattat atatgcttgc attaatatct ttttctatta ttagtattat atttttatca 2041 ttatttatat agtattcttt tatactgttt tttataatat tttttccata aattactcct 2101 ttttttattg atttatataa gatttcctcc ttctctaaat ttatcaacct tttctttaag 2161 tgtatcgtat aaccttagaa aactcggtag agataaatat acagtagtat caacatctac 2221 agtattttct tttctcattt catttccaag caaaagcact atctcttcat cattttgtat 2281 tacagcactt ctgttgctat atatacttct tgattttttc atattgtata ttgtattgtt 2341 tattttcata ctatctcctt ttatagttct atatataatt ttataatgtg aatgtattta 2401 ataattttca gtgtatttaa aatatacttt ttattttcaa taagtcatta atgtgaagta 2461 atatatagtt cctataaatc ttaaaaagta tatattttat aaaaaaatcg aattttataa 2521 gcatttttta gtgaaaacga gtatgaattt atttgtttta aataaaaaaa taaaatgctt 2581 gttttttata gatttttatt atatcatata atattagtaa aaaaataaga taaagtttaa 2641 cgttttaagg aaagaaaatt aatttatgaa aaaaattgta ttgtttatta tattgctaag 2701 cattcaagcc tattcacaga taatgactcc tgaaaaagtt gttagtatga tatctaatag 2761 attttcaact ataaaaggtt tttcagctaa ttttgtagag agaaatggtg aaagaatttc 2821 ttatggtaat gttataacta aaaatcctaa tttgtttaga atggaatatt caagtggagg 2881 aaatggacag tctatatact gtaatggtga aactttatgg ataatatttc caaaaaataa 2941 tgttgtagca gagcagaaat taaattatgg tgattcaggt gctatatata ctccggaagg 3001 aattatgagg cttacaacta aatttaatat agatttttat aatgaaagag aattggtatc 3061 tttaaatagt tttaaggatt ctgaactagg aatagctaaa tataacagtt cttatgcagc 3121 aaatgataac agaagggctt atcatatgct tttgacttca aaacaggcta gtgtagacag 3181 aactggcttc acaaagatac atttatgggt tgataataca ggtatgatca taagaatatt 3241 aggaataaca actactaatg ttcctgtaga gtatttattt acatctatta gatataatcc 3301 taaatatgag gacaataata aggattttga accggaaata tcagaaaata tgcaggtact 3361 taaagatggt ttagtaccta acaattaatt ttaaggattt atatggaaac tataggtcaa 3421 atattaaaaa atgctagaga aaagaaaggg cttactatag aagaacttga ggctactact 3481 catatagttg ctaggtttat aaaggctttg gaaaatgaag aatttgatgt tcttcctggt 3541 gaaatatatg taaaaggctt tataaagaac ttaagtgata aattgtcatt agatgctgat 3601 atggtgcttg aaagatataa tttgcagaaa aacggcatca agtctgaaca ggatttattg 3661 aaaagcggta aagctattaa aaatattaaa aaaaataaag agcctgtaaa agaagttaaa 3721 gacaataaag atattaatga tggtaaagat aataaagaac aagaagttaa taaagaacca 3781 gtaaaagata ctaatacaaa agaatctctt aaagatgtta taaataccga aaagcatgaa 3841 gaaaaaacta taaaagaaac aaataagaaa aataaaaata ctaatactaa taatcgtgct 3901 gaagcagatt tattatttat ggcaaggaga gatttatata aattaagaaa taaaaataaa 3961 tctataatac ctacagttat tattatactt gctatcatag ttgttatagc tgttatagtg 4021 tttaattgga agaatataac aggatttata ttcccttcaa ataataaaag acaaaacaga 4081 aatgaagttg agatatcaaa aaatatagtt gacagtaagg ctaagcaaag cgttaaatca 4141 ggagatatta tatattttaa gcctcttgga atatcagcta caattaaatt taatagtata 4201 ggtaatgtgc ttcatattaa tattaacgga caggatttat ctttctctaa gagcagtcct 4261 ataatattgg atttaaatgg aaatggaata aatgatttta agataagtgt tatagaagtt 4321 tatgataatt cagctactgt agaaatggaa aaacttgaag aaaatcagat ggttaataca 4381 ggttatacca atgatataga gtctgctgct atgaatacca caaattataa taacactgta 4441 tctacaaatt tacttgtgat aaatggagaa acttatatag aacaagatac tgaaaaagta 4501 gatataagaa tagagataac tgctaagcat tttgtatata taagatattt tatagattca 4561 aacagaccag ctactactaa ccttttaagc ggtaaaacat tatatttaga agctaaagat 4621 gttgttatgc ttactatagg taatgcggga gaggttgtag taaaagttaa tggaaaaatc 4681 attaatgtag gtgctgcagg agaaactgtt aataagacta taaaatgggt taagaatttg 4741 aatgattcta ctagatataa tcttattatg tcagatacta agtaattatt tatttagtat 4801 tttaatattt ttgcagcata gtatcataat ttaatattaa aaaggaaatt taatttgcaa 4861 aatatatatt tacatagttt aggatgcgag aagaatacgg ttgatggaga gcatatatta 4921 gccatattac agaaaaaagg ctttaatatt accaataatc cggaagatgc agatattata 4981 gttataaata cttgtgcttt tatagaggat tcaaagaaag aatctattga tgctatattc 5041 gatcattcta tgtacaaaaa atacggaaaa tgtaaaagat taatagtatc aggctgtatg 5101 agcgagagat ataaagaaaa ttttcttgag atgttcaagg aagttgattc tgctataggc 5161 atatatgatt tagaaaatat attaaaagcc gtagaagaag atggttttca tgatgcaaaa 5221 gaaaatactg aatataaaga gtatggcgat agagtaaata ctggtaccaa gtatagcgtt 5281 tatataagaa taagcgatgg atgtcatgca aactgcagtt tttgtgctat acctggaata 5341 aggggagatc atagaagcag aaagatagaa gatattgtaa aagaggctga aaattatgct 5401 aaaaacggag ctaaagaaat taatttaata gctcaggaaa cgactttcta cggatatgat 5461 atatacaata aattagcatt accagatttg cttaaagctc tttcaaaggt tgaaggcatt 5521 caatggataa gagtattata tcagaatcct gttgtcttaa atgatgagat aatagaatca 5581 ttctttaata cagataaagt tgttccttat tttgatatac ctttgcagca tgttgataaa 5641 gatatattaa aagatatgaa cagaggcaca agaagctata aattttatag agaaatgata 5701 gacaaaataa gaagttataa taatgatgct gttataagaa cttctttgat agtaggattt 5761 cccggagaaa cacctgaatc gtttaaaaag ttaataaagt ttgtaagaaa tgccaagctt 5821 gatagggtag gggtattcac ttattcagaa gaggagaata ctaaggcgtt acttatagat 5881 aaacctaaat tgagcagaaa cagaaaatta atgcttagag aaaaacttat gagagcagct 5941 atagaggtgt ccgaaaaaag attagagaga tttataggaa gaactataga tgtattgata 6001 gagaaaaaag aagatgagaa taaatttata ggcagaagca tatacgatgc tcctgaagtt 6061 gatggatatg ttgaagttta taatgaaaat actaataata atataaatat tggcgatata 6121 gtgcaagtta ctgttactca taatacaaaa tatgatttaa taggtaatgt tatttaatta 6181 ggagagcttt tgctatgaag aaaaaaaagc aggaaaatga acctagcagt atagaaaaag 6241 aagaaaaaaa atcatcttat gctaacagag aagatgcttt tagggcatat tttgaaatag 6301 ttacaaagat agtttataca ttgaatttaa tatcagacag aaatgacatg actagaagac 6361 tttcaagcaa tattgataga gctttgggtt atgatgatga aatgatgtct aataatgaga 6421 aaatagccaa taacagcaat gaagttgaaa tacttataaa ttctcttaaa acagatgtaa 6481 ggaaaatatc cacttttaaa aagtataatt ttaatataga aagatttatt tcagataaaa 6541 atttagattt agatgagaga tttattttat attccgtatt agtttatact atttacggtg 6601 attctatgtc agcaatatct gttagaagaa tattagagct tattacattg aataatgata 6661 tatatataaa taagtaccat tattttgata ataatagtaa attgatatca agcggtatat 6721 ttaatttatc gcatgaacct tatccaagca gcggtatact tgaaatcaca aatacattga 6781 taacatttat aagttctaaa gtgatattcg catttttgga aaaagataat tataatgtga 6841 tagaaagtat aataagcagc ggagaggcta taaagaaaat ttcattagaa aataagatag 6901 gaggtaaacg taaatcttcc gaatcagata ttttaactcc taaagaaata gtagctcaat 6961 tagataaaac agttataggt caagacgagg caaaaaaagc attagccgtg catgcttatt 7021 tgcattgttt gagaataaat ggcaataaag atataccatt cagatcaaat attcttatga 7081 tagggcctac aggagttgga aaaacttatt tagtaaaaac attggcagat atattgggac 7141 ttccttttgc tcgtgcagat gttaccacac ttacagaaac aggttatgtt ggtgatgatg 7201 tagaggtagt tctttataat ctttatagaa aagctaatgg tgatttggag aaagcccagc 7261 atggtatagt atttttagat gaggttgata agatagcaaa ggcagatgct catcaatcaa 7321 ccacaggcaa tccttcagat aaagcagtac aggaagctct tctttctatg atgaatggtg 7381 aagatataag agtacctgag tttggcgaca gaagaatgat gcattcaagc gacggaatac 7441 taatgaatac taaaaatata ttgtttatat tcggaggtgc ttttgtaggt cttgatgata 7501 tcattaaaat gcgtttgaag ggtgaaagca gtttaggttt tggttctaat gctgttataa 7561 ataaactgca gaaaaatagg atattaagtc aggttgatgt taaagatata gaaaaatatg 7621 gtatgatacc tgagtttata ggaagaatac ctataatagt aacattgaat gatctcacta 7681 aagaaaattt gaaagatatc ttgacaaaaa cttcagaatc tcctattata aaatatactg 7741 aatttttcaa aagtataggt aagaagttgg tagtaactgc agaagcaatt aactttatag 7801 ttgataaggc ttctagcatg aatatggggg caagatcttt aaaaagtata gtagaaacag 7861 caatggttaa tatacttttc aatttagacg gagtaaaagg caatacctta actcttacta 7921 aatatgatat agagaatgca ttcagtgaga aagagtttgt aaattcaggt aatgaagata 7981 aaaaaacaga taattctaat aagaattttt ctaaagaatc ctttatggct tgattttact 8041 ttatgtttgt ttatttgtta taaataatgt atatataaat tatttaagaa caggtaatat 8101 attggagttt gtagagatga tagtaactaa agaaaaagaa ttattaaaag attatgaatt 8161 aatagaagca cataaaaaag gtgatagtga agcattagat atattattaa caagatataa 8221 aaactatata tttaatttat catatcgttt tatgcataat tatgaagatg ctatggattt 8281 aacacaagat gtattgatac gtgtatataa agctatagac agatatgaag aacgcaatta 8341 ttttaaaggt tggttatatc gtataataag caatacagct ataaacatgt attctaaagc 8401 ttataggaga gaatcacctt atctagaaaa actagaaata gttgatgata aaaggtataa 8461 tacagaaaaa gaatatgaaa gaatatacct tcaggaaaaa atttacaatg cttctactaa 8521 attaaaggga aaacagagag atgtctttat attgagatat tatgagaatt actcatataa 8581 agaaatatca gatattttga atatatcaag cgattcagct aagagtaatt attcctatgc 8641 tctcaagaaa atgaaatcgc ttctagaaaa agaaaggact cttttatgaa actacatgac 8701 atgatgcaat ttaaaagcaa aatcgaaaag aaacgacatc attttgattt gccaagcttg 8761 cctgatagag aatggcagta catgttgcaa agggctaaaa tggaaagtat aaaagaaaat 8821 gaaattagcc cattgtatgc ttttatgact tctgctagac tttctatagc tctatctaca 8881 gcaatagttt tggtattagc tttatctttg ttttatggaa atcctaataa agaggattct 8941 cataatacca atactgcatc atctagtaaa actaacataa atgttgttaa tgtagctaat 9001 agtagtttta tttaaaattt aaaacctata atagaaattc taaattatac attaacattc 9061 ctatatgtta atgtataatt ttattttatt tataataaat agtatttcta ttatatccga 9121 taataattat taaaaaatat atttttttat atatgaaaaa ttatatatat acaggactgt 9181 aatatgtttc aatttcattt aacaagtaag gcaaaaaagg taatagaatt atatgcccag 9241 gaagaggcta aaagattaaa tcatgatatg gttactcctg aacatatact tttggggctt 9301 cttcatgaat cagaagcttt ggctacacgt gttttattga gattgaaaat tgatttggac 9361 agacttaaat tagaattaga atcagctatg gtaaaatctt caactacaaa agtatttgga 9421 actttaccta ctgctcctag agtacagaaa cttataagca gatctgctga agaggctaga 9481 gctttaagcc ataattatat aggtactgaa catttacttc ttggactttt aagagaagaa 9541 agcggtacag cttataatgt actaacaagt atggggcttg agcttactat attaagacaa 9601 gaaatattaa aaatgcttgg tgttgcagga agcaatattt cttctatgga acagactact 9661 caagaagata atgttaaaaa agttaaaact cctactttag atcaatttgc cagagattta 9721 actaaaatgg ctagagataa ggctttagac agagttatag gcagagaaaa tgaagttatg 9781 agggtagttc agattctatc aagaagaaag aaaaataatc ctatacttct tggagagcct 9841 ggggtaggta aaacagctat agttgaagga cttgctgaaa agatagtagc ggctgatgtg 9901 cctgatatac ttcttaaaaa acgcgtatta actttggatt tatcttctgt tgttgctggt 9961 acaaaataca gaggtgaatt tgaagagaga ataaaaaata tagttttaga aataaaaaaa 10021 gctagtaata ttattatatt catagatgag cttcatacat taataggagc aggtggtgct 10081 gaaggtgctt tagatgctgc taatatgtta aagccggcac tttcaagagg cgagattcaa 10141 tgtattggtg ctactactat aaatgaatat aaaaaataca tagaaaaaga cggtgctttg 10201 gttagaagat ttcagcctat caatgtggaa gagcctagca tagaagatac tatagaaata 10261 ttgaatggta taaaaggtaa atacgaagaa catcataaag taaaatatac agatgaagct 10321 gtaaatgctg ccgctgtact aagtaaaaga tacatttttg aaagacattt acctgataaa 10381 gctatagatt taatagatga ggcaggttca agagcaagac ttcttaatat gacaagacct 10441 caagagttta aagatttaga taagaaaata gaagaactta atcagcaaaa gaaaaatgct 10501 gttgataatc agaattttga agatgctgct aaaataaggg atgaaattag ttctatacag 10561 gaagagcttg ctaagaaaga agaaaaatgg cgtgaagaaa gagaaaagat agagacattc 10621 attgaagaag atgatataag acatgttata tctgaaataa ctaatatacc tataaaaaga 10681 ttattaaatt cagaaagcaa aagattaata ggtatggaag aagaactaca tcagaaagtt 10741 gtaggacaga aagaggctat atcttctata tctaaggcta ttagaagaag cagagcagga 10801 cttaaaacat caaaaagacc acttggtagt tttatattct taggccctac tggtgttggt 10861 aaaacagctt tagctaaagt gctttcagag tttatgtttg gagatagcga tgctcttatc 10921 agaatagata tgagtgagtt tatggaaaag tttgcggtaa gcagacttat aggagctcct 10981 ccaggatatg ttggttatga agagggaggt ggacttactg aaaaggtaag aagaaagcct 11041 tattctctta tacttttcga tgagatagaa aaggctcacc ctgatgttac taatatactt 11101 ttacaggtgc ttgaagaagg tcagcttact gataattttg gaagaaaagt tgatttctca 11161 aatactatta taattataac aagtaatttg ggtgcaagag atattattaa aggaagttct 11221 ttaggattta atgctgttgg aagcgaaaaa gatgctaatg atattaaaaa tttcgctttg 11281 gaagaattaa aacagaattt caatcctgaa tttttaaata gaattgatga tattatagtg 11341 ttccatactc ttagcaaaga ggatttaaaa gatataatca atataatgct taaagagctt 11401 aatgaggcta ttaaagagag aaatatcgtt atcaatttaa gcgaagaagc taagaattat 11461 attattgata agggatttga taagaagtat ggtgctagaa gtttaagaag ggctatacag 11521 aaagagatag aagattatgt gagtaccgaa atattgtttg gtaatattga agatggtgat 11581 actattaacg ttgatgctaa tgatggctct ttgatatttt cttatgagaa gtctactaag 11641 actgaaaata aggaattatc taaaagttag ttaacatact cctataaata tttgtgatgc 11701 ttccaaaatt atttttatga ttttggggcg tctttttatt ttttagtttt tatataatga 11761 aaattagtca tttgtgaata ctttctttat tctataattt gtgaaatctt tttctatatt 11821 ataatctata gtaattatat atggtctagt attagtgtat atatagctta tatctataat 11881 aatatcattt atttcatagc cttttataat gctttctctc cattccattt cagtattttt 11941 catttcgcct atatttaatt ttttatttat tggtataata ttatctatat taggagagcc 12001 tccgaattgc cttcctatta tatgtccgcc ttgatcatga ttttttatgc cgtagagttc 12061 cacaactttt ctttgagcat aattatttct tttagcattt gaaagtatga gattatcttt 12121 tcttatgact ttatatattc ttgattgttt gtctgtactg tatagtgtgc catttatatt 12181 gtattttata ttaggtttta gatattctat tctatttatt tttacaaaat gagatttatc 12241 atcatcattt tttatattgt atacctgatt agtttggcat gatattataa aagttataga 12301 gataataaaa caaatatata ttctcatatg ataatggatt atatttttta tttctttact 12361 tacaaccttt taaaagatgt atttttaatt ttttattatg aaaatacatt ttatatacgg 12421 tatttctata tggaacttta tataaataat acaaatgttt ttttaattag ttatataatt 12481 atcaaaataa ttttagttaa aatgatgttt tatgtattgt gtaaatcttt aatacacaat 12541 taatatatgt atacttaaag tttattagct ttagtgcata gtagggtaaa tagagaatta 12601 taaatatata taatcaaagc agtttaaata tttgttagtt ttgaaaaagt ataattatgg 12661 atttataaag agcaataaaa aagctatgta tgaatattgt atccatacat agctattatt 12721 tttaataact gttattttta gttatttgtt tgagtagtac cagcattatt ctgctgttgg 12781 tttcttaata tcatagcttc atcataagtt atatattcca tattaggatc ataagtaccg 12841 gctctctctt caccattcat actaggtata gttaatacct gtcctggtaa tattaagtct 12901 gggttattag gatctcttaa tacgtttcta ttagcttgat ataatctata ccattgtata 12961 gggtcattat atatgaatga ataaccagct attctccata aagcatcagt taaaggagtt 13021 ctttgtacta cagtgtaata cataggaaga acagtaactt taccttcagt attaacagtt 13081 tgaccagtag tagcatctat tatttgacca gtttcattat tattgttatc tgtatcaact 13141 aaatcaccat ctacaggtct cttaggtccc atttcatata tagcattcat agttctatta 13201 gcttcttcta gtttttctct agctaaagta ttatcattat tagctaaagc atcttcagca 13261 tcttttaata tttgagaaag attttgatcc tcttcactat ctggtattat ataaccttca 13321 tcaactaatt gttgatatct agctttaaga tcatctaatt gtctttgatt ttcttctctt 13381 gctatagtat tatcacgttc tgtaactaaa tcgtccatag ccttagaagc ttccaatgct 13441 ttttgactag cagcagcagg atctgtttct actacattat tagcatcagc taaaagagta 13501 gatatattct gatcagcttc atcacctctt gttatagtgt tgtcattaac taggccatca 13561 tatttttgct gtgcattagc aatatcttgt ttagctttag cgatagcatc agctttttct 13621 ttagcaagtg cttcttgttc tgcttttttt ctagcttctt cttcagcttt tttcttagct 13681 tcagcagcag ccttagcttt tgcctctgcc tcagcttttg ctttagcctc tgcagcagcc 13741 ttagcttttg cttcagcagc agccttagct ttagcctctg cttcagcttt tgctttagca 13801 gcagcggcag ctctttcagc agcagctttt gcatctgctt cagctttagt tataccagca 13861 agagcagcag caacattctg ttgaacgctt gcaaaattat cagcttttaa agcttcatct 13921 gctttagtta aagcacctga aactgtttta tcattattgt cacctgcttt tatactttta 13981 cctaaagcag cattgtattt acctctagca tcagctattg ctttagaagc agtgtctctt 14041 tctcttccta aataaccttc tttaactgaa ttagcagaag ataatgcatt attatagtat 14101 tctaaaacag tattgaaagt attagaagcg gtattgaact cttcatcagt ttgtgctact 14161 gtatcaggac caggtatgct tcctatatta gcattacctt cttcaaaata tttaacagct 14221 tcttcatact gagttttgaa ttgattatca gtagcagctc ccatttgatt tatttcagtt 14281 attgcattat tagcatcatt tttagtttta tttattctag cattcataag tctatagaat 14341 aatgccattt gggctttatc tatacttaaa gaagcctctt gagacttatc ataagactgc 14401 tgatattgac cttgattctg aagatcatca gcttcttgag ttattctagc agcatcttca 14461 taagtctgtg ccggtaaaat agaccatgtg aaagataata aagctatagt agccattatt 14521 ttttttattt tcatcattta ctccttaaaa atattagttc tgttcctgtt caaaggcttc 14581 tatttcctgc atcatctttt ctaattgagc tagacgttct ttaactaatt tcaaagcctc 14641 atcactttta tcaaattgtt ctttggtttt attatataaa tctgtataag ctactttggc 14701 ttgataaagt ttatctacca tcactctgta gtctttagtt tcagctgtat acattgcatt 14761 agcttcctga tatacagtat cagctttagc aaattcatca gcgtacatta caccagcttt 14821 aatatcaata gcattagttg cactttcatc agtctgtttt ttgagtattt cagaatatgg 14881 tggcaaacct ttgtctaaaa ccaaattata attagtttca gcagctaata ataattcttt 14941 agctttttca gcttcgtctt tgttttcctc tttcattatg atatcagctt cagcatagcc 15001 tttttctgct atatcaaaat cttcctgagc aaatgtttgt atctctggat atttgtttac 15061 tacagtatct cttagagcgg ttaccctttc atactccttt gtaggcaagc cgccgcatga 15121 aataagcata aaagctatta aggtgataaa acctatttgc aacttcagtt tcataatcag 15181 attaatcctc cagtatttat aaatatattt tatggctttc aattaagtaa aaccataatt 15241 attaaaataa gaaaaaaata taattacatt ttatataaaa aaaatgaaat tatgttaact 15301 atgttattat taacggtata aaagtcaagg gcttttatag ttttttactt tatttatgta 15361 taagattatt tatttttgta aaaaaagaga ctttttaaat ataaagtctc tttttttaaa 15421 aatatttgtt atatttatta tcctaattat tatttattag ataagtattt atctattcct 15481 gcagctgctc ttttaccatc tccaatagct aaaataacag tagcagcacc tctagcagca 15541 tctcctccag caaatactcc ttccatagaa gtagcacctg tctcctcatt tataacataa 15601 gtaccctttt tagtagtttc taattcagga acttttctag ctataagtgg gttaggagta 15661 gtacctattg ctactataac agcatcaact tctatgtctt ctgtttgtcc ttcaacagca 15721 acaggttttc ttcttccatc agcatcagcc tctccaagct ccataacttg agttctaaca 15781 cctataacat tgtcattttc atcgcctaat atttctaaag gagctcttaa gaatctaaaa 15841 tcaacacctt cttccatagc atggtgaatt tcttctaatc ttgctggaag ttctttttca 15901 gttcttctgt atatgatgta tactttttca gcacctaatc taacagcagt tctgcaagca 15961 tccatagcaa cgttaccacc gcctaataca gctactcttt tatgttttat aataggagta 16021 tctacttcag ggaatttata agctcccatt aagttaactc ttgttaaata ttcattagca 16081 gagtaaacac cgcataaatg ttcaccaggc acattcaaaa atgcaggaag tccggcacct 16141 gttcctaaaa atatagcatc gtattctttt cttaattcat tgaaatctaa tgatatacca 16201 gcaacttcat taattctgaa gtttacacca tcttttttaa ggttttctac ttcatgtgct 16261 accaattctt taggaaggcg gaattctgga ataccgtaca tcaatacacc acctattgtg 16321 tgtaatgctt ctaatacagt aacatcatat cctaatttta ttaagtctcc agcacaagct 16381 aaacctgcag gtccagcacc aattatacat acctttttac catttttttc tacttttaga 16441 tcctcatgct gagcatgttt tctttcatag tcagcaacaa acatttctaa tttaccaata 16501 gcaacaggtt cgaatttttt acctactaca catctttgtt cacattgctc ttcctgaggg 16561 catactcttc cgcatgcagc aggcaaagca ttagtttctt taattttttt agctgcttct 16621 aaaaattttt tctctgctat aagtcctata aattcaggaa tttttacctt agcaggacaa 16681 ccttccatac aatgtggaac tttacaatct aaacatctta aagattcttg atatgcttgc 16741 tcttcagtat atcctagagg tacttcttta aagtcttttc ttctttcctc agggcttcta 16801 gcaggcattt cttgtctagg tatttcgcct aatttcgatc ttggtggcat atatatctct 16861 cctattttat attttaggta tttttgcttt tattttcttt agcaaagatt atactataat 16921 tttaaatata atcaatgcta atattatttt ttatttttaa gttttttatt attggattat 16981 atactgaatt taaataaata ttatcgtttt tattttgtga tttaaatttt attttgtaaa 17041 ataaaataac cagtcattta ctttaatatg aataaatgac tggttgtata ttataataat 17101 tttataatat agatattatt ttatactgtt tatagcatca tctatcattt ttgcacattt 17161 ttctattttt gctttatcat catctattat attgcttaat ggttttctta ctccgcccaa 17221 ttctagacct tttaatttaa gaattgcttt cattacagca tataaattac catgacattc 17281 gcacatagca tatatgattc tgtctatttt gtattgtata tctcttgctt tatcaaactg 17341 gcatttatta acggcatcgt tagcagctaa gaataattca ggcattacag cataagtacc 17401 tcctattcca gcatcagcac ctattaatct tccagctaca aattgttcat ctggtccgtt 17461 aaatataatg ctgtcatcac cgccaacatc tttaaacatt tgaatatctt gtacaggcat 17521 agatgagttt tttaccccta taactcttgg attttctctc atttttttat aaagattaac 17581 atttaaagca acaccagcaa gctgaggtat attataaata ataaaatctg tattaggagc 17641 agcagcactt atatcattcc aataatctgc tatagcataa tcaggaaggt ggaaatatat 17701 tggaggtata gaagctatag catctactcc caatttttca gcatgagcag ctaattcagc 17761 actatcagca gtattgttgc atcctacatg tgctataata gtaattttgc cttttgctac 17821 ttccataaca ttttctaata ctaattttct ttcttcttta ctatggtaaa tacattctcc 17881 tgaagatccg cctacatata agccgtttac acctttgtct attaaatgct gtgtaaactt 17941 tttaactctt tcagggctta tttctccttt ttcatcataa caagcataaa atgcaggaat 18001 aattcctttg tacttatcta ggtttctcat //