LOCUS NC_017179:8 18736 bp DNA linear BCT 04-SEP-2013 DEFINITION Clostridium difficile BI1, complete genome. ACCESSION NC_017179 SOURCE Clostridium difficile BI1, complete genome. COMMENT locus start: 1378875; locus end: 1397610 COMMENT n1_4mer:GRV/n1_4mer:RV = 5.728208; n0_4mer:D = 25.539696; n0_4mer:PS = 43.850753 FEATURES Location/Qualifiers source 1..18736 /organism="Clostridium difficile BI1, complete genome." gene complement(1..738) /gene="" /locus_tag="" /db_xref="1378875..1379612" CDS complement(1..738) /gene="" /product="NAD-dependent deacetylase" /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN TVYDKSASLVINKPIGEVLYEAVLRQI" gene 953..2776 /gene="" /locus_tag="" /db_xref="1379827..1381650" CDS 953..2776 /gene="" /product="mannosyl-glycoprotein endo-beta-N-acetylglucosamidase" /translation="VKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK" gene 2991..7289 /gene="" /locus_tag="" /db_xref="1381865..1386163" CDS 2991..7289 /gene="" /product="DNA polymerase III PolC-type" /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF" gene 7493..7954 /gene="" /locus_tag="" /db_xref="1386367..1386828" CDS 7493..7954 /gene="" /product="hypothetical protein" /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF" gene 7984..9138 /gene="" /locus_tag="" /db_xref="1386858..1388012" CDS 7984..9138 /gene="" /product="transcription elongation protein" /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE" gene 9151..9426 /gene="" /locus_tag="" /db_xref="1388025..1388300" CDS 9151..9426 /gene="" /product="hypothetical protein" /translation="LKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGRAN GRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK" gene 9416..9727 /gene="" /locus_tag="" /db_xref="1388290..1388601" CDS 9416..9727 /gene="" /product="ribosomal protein" /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE I" gene 9749..11689 /gene="infB" /locus_tag="infB" /db_xref="1388623..1390563" CDS 9749..11689 /gene="infB" /product="translation initiation factor IF-2" /translation="VSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN YNDIKEGDIVEAYITKEVERKL" gene 11732..12112 /gene="" /locus_tag="" /db_xref="1390606..1390986" CDS 11732..12112 /gene="" /product="ribosome-binding factor A" /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM YMDNLIKRVNEKNAQQNEDENYDE" gene 12105..13076 /gene="" /locus_tag="" /db_xref="1390979..1391950" CDS 12105..13076 /gene="" /product="RNA-binding protein" /translation="MNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTLKNLG KNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEIKNNA SKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIATCLYT GLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEALKGL EIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEIKVSF RSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI" gene 13089..13994 /gene="" /locus_tag="" /db_xref="1391963..1392868" CDS 13089..13994 /gene="" /product="tRNA pseudouridine synthase B" /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST DNFLGLGKLSKHNNTISVKSDKMFI" gene 14125..15054 /gene="" /locus_tag="" /db_xref="1392999..1393928" CDS 14125..15054 /gene="" /product="riboflavin biosynthesis protein" /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK" gene 15158..15415 /gene="rpsO" /locus_tag="rpsO" /db_xref="1394032..1394289" CDS 15158..15415 /gene="rpsO" /product="30S ribosomal protein S15" /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK" gene 15655..16500 /gene="" /locus_tag="" /db_xref="1394529..1395374" CDS 15655..16500 /gene="" /product="hypothetical protein" /translation="LKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA ISCSN" gene 16625..18736 /gene="" /locus_tag="" /db_xref="1395499..1397610" CDS 16625..18736 /gene="" /product="polynucleotide phosphorylase/polyadenylase" /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID EKGRVNLSRKVLLPKPEHKNK" ORIGIN 1 ctatatttgt cttaatacag cctcatataa aacttctcca ataggtttat ttattacaag 61 agaagctgac ttatcataga ctgtatttgc tttattaata agagcaatat aatcaccttt 121 gtaataatct ataaaacttg ctgctgggta cacagctaat gatgtacctc ctataatcaa 181 taaatcagca ttacttatag cagaaattgt tttagtaatg acatcactgt caagagcttc 241 ctcataaagt actacatctg gttttactat agaaccacag ttgtcacaat aaggaatatt 301 acctcccaga tttaacatag attctaagtc aaaaaactta ccacatttag tacaataatt 361 tctatgtaca gaaccatgaa gttctagcac atttttactt ccagccattt gatgaagtcc 421 atcaatattt tgagttatga cagctttaag cttacccatt tcctcgagtt ttgctaaagc 481 tatatgagca ttgttgggtt ttgcattagg atatattaat ttatccttat aaaattcaaa 541 aaattcttct ggatatctaa caaaaaaagt atgtgaaact aattgttcag cagtaaattg 601 tttattgagt ttttggctaa ataatccagt agaacttcta aagtctggaa tattagattc 661 agttgaaaca cctgctccac caaaaaatac aatattattg tgattagcta ttaagtcttt 721 taagctattt gcatccatag ttacacctcc aaattattat atataaattg tactacatat 781 taggtctttt atgttttatt aatttaaata aatatgtagt ttaataagtt aaataaaggg 841 aatataaata aaatgtatac agagtataaa tagacaatat ttactaaaat aacaagttgt 901 cgtataatta tattaaaatt gttttagtgt ataagaaggg cgggggaagt acgtgaagaa 961 aaaagcagct ttagcaacat tggctatgtt acccttaggt gtggtaaatg cacatgctga 1021 tggagatata ggtatagtga ctataaatta tttaaatgta agaaatgagc caactgccga 1081 aagtagcata gcctttgttg ctaaaaaaga tgataaagtt ttgattaaag actcttctaa 1141 tggatggtat aaaataaaag ctgaatctgg acaagaaggt tgggcttcat caaaatatat 1201 agcaaaatca aatagtgact ctttaagaac gtctacaaat aaagaaaaac aagttatttc 1261 aaatagttta aatatgagaa atggagcagg tacaagttac agagtaataa ctgtactaaa 1321 aaaaggtcaa aaagtagaag taatatcaga gagtaatgga tggtctaaaa tcaaatatga 1381 tggaagacta ggatatgtat ctagctctta tttaggagac gtttcaaatt caactaataa 1441 atcaaaaact aaacaagtga atacaacttc actaaatgta aggagtggac caaatacaag 1501 ttatggtttg ttaggcaagt tgccaaaagg aagtaaagta gaagtaatat cagaaagtaa 1561 tggatggtcc aaaataaagt ataatggaaa agatgcctat atatctagta tgtacttatc 1621 ggatgtaagt caaagtaatt cagacaattc tagtcaaagc aatgataaaa agaatactga 1681 taaggttgta aatacagctt ctttaaatgt aagaagtgga ccaggttcta catatagtaa 1741 gttgggaaaa gtttataaag gaagtaaagt aactgtacta tcagaaagta gtggatgggc 1801 taagattaat tttaacaata aagaagcatt tgtagtaggc aattatttat ctacttcagc 1861 agatacttca aataataact caaatagtaa ctctgataac agttctaata gtaatggcaa 1921 taattcttca tcatcaggtc aagtaaatgg tatgtcaggt ataagtgggg ctaaaattga 1981 ttataaatct ttgagttata ctttagaatc tcatataagt aaacaagttg agaaagcagc 2041 atcaggagga aatgtgatag ctccaagtaa taggaaaagc actccaagtc ctgaatttag 2101 tactttttca gctcaaagaa caagctcatt tgtaaatgca agttcaagtg atatagaata 2161 ttatttaaat cctaaaaatt ttacaaatac tactaaaggt atgatgcagt ttttaaagat 2221 taacagttat agagatggta tttcagaatc aagtcttaac tcgtatctta atggtttatc 2281 ttctagtgta tttaaaaacc agggagcagc ctttataaat gctgccaaga agtataatat 2341 agatgttgta tatctagtat ctcatgctat gtgggaaact gcttatggta agtctacact 2401 tgcccaaggt caaacattaa catcttataa gggacaacct cttagtaagc cagttaaagt 2461 atacaatttt tttggtatag gagcaataga taaaagtgct aatgtttcag gtgcagaagc 2521 agcgtattcc aatggctgga ctagtgtaga agctacaata gatggctctg caaagtggat 2581 atcacaaaat tacgtaaata gttctaagta caatcagaat actatctaca agatgaagtg 2641 gaattatgat tatacatggc atcagtatgc aactgatgta aactgggcta atggaatttc 2701 tggaattatg gaaaatttaa ttggtcttta tggaggagga agtagtttgg tttttgaagt 2761 tcctcaatat aagtaataca taaataaaaa atatagacta tgggtagttt tcattttatt 2821 taaattatct atagtctttt tgtatataat ttataatctg taaatttaat gttaacaaaa 2881 cttttatgtt ataatattaa ttctaagtaa atataagtga gtaataggta gaaatagata 2941 aaaagtggta ataatatata taaagataat tttgagaaag gaggatatat atggaaagta 3001 ttaaggaata tttggataag ttagaaatta ataatagtgg tcttggaaag caacttaaag 3061 aagtttatat aaatagagta gtttatttta aggaagataa gatagtttat ttttatctaa 3121 cttctaaaga tattgtttct catgaacttt tagataaatt taaagaagaa ttgatgtata 3181 aactcgatta ttttaaagat atgaggatga agataagatt tactggtctt gaaagaaaat 3241 ccaataaaga tgttataaaa aaatattgga ataatatatt gtatatatta aagtatcttt 3301 gtccatctat tgcagggtgg tataaacaag tagaatttct ttgtttagaa gaagaattaa 3361 aaataaaact tccaaaaggt atattttatg agcgattaat gaaaaaaaat gtaacttatg 3421 tacttaaaac cgtgcttagc gaagagttgg gattagattt aaatataaca atagaaaaag 3481 ctgttgatga aaaggtaaat aaagaaagac tcataagaat caatgataga gaaatggaag 3541 aaaaaattag ggctttagaa attggaaaag ttaataactg tgaagaaaat gaagaggaaa 3601 gctatgtcat taagtctgat gtagatgaaa atttaatcta tggagataat gcaaatgcta 3661 tgattgaaaa tatagttgaa ttaaatgcat cttctggaac agttgcagtt gttggagata 3721 tatttgatgt tgacactaaa gaacttaaaa acggaaaaat acttatgata gcttcaatta 3781 cagattatac tagctcaata agctgtaaac tttttttaac agatacaaat aaggatggag 3841 tacttgaaaa tgttaaaaag ggagcatact taaaaatcaa gggtgacata gtttatgata 3901 catatcaaag agaaattagt atgatgatta gtgggataag aaaagaaact agaatagaga 3961 gagaagataa atcagaggaa aaaagggtag aactccatgc acatacacaa atgtcttcta 4021 tggatgctat ttgttctgtt aaaaagcttg tagagcgtgc agccaaatgg ggtcattctg 4081 ctattgctat aacagaccat ggtgttgtac aaggatttcc agatgctatg aatgcaggta 4141 agtcaaataa tataaaaata ctttatggag tagaagggta cttggttgaa gatgattctc 4201 tcattataga ggatgcaaat gataaagaac tttcgcagac atttgttgta tttgacatag 4261 agacaactgg tttttctaat acaaatgata aaataactga gattggtgct gtaaaaatag 4321 aaaactttaa gattgtagat agatttagtg agttaataaa tcctgaaaaa gatatttcct 4381 ataagataca ggaattgaca ggaataacta atgaattaat taaagacaag ccaaccattg 4441 aagaaattct tcctaagttt atggaatttg ttggagatag tgtattagta gcacataatg 4501 cagagtttga taccggattt atatcgcaaa aatgtaggga acaaggcttg ttatataata 4561 acaaaaaagt agatacactt atgttagcaa gagtaatgtt gccaaactta aaaagacata 4621 gattaaatgt cgttgcaaaa gaacttggta ttcctctttt aaatcaccat agagcagtgg 4681 atgatgctga ggctacagca cttatattta ataagttttt gcagatgctt acagaaaaag 4741 gtgcaaaaac tctaagtgat gtaaacaata tacttggaaa gattgattac acaaagctta 4801 gcacaaatca tataaccttg atagcaaaaa attctatagg tataaaaaat ttatataaaa 4861 taatttcaga tgcacatgta aatcattttt ttagagcacc aagaatcctt agaagtgtat 4921 taaatgaata taaagaagga ttaataattg gttcggcttg tgaagctggg gtggtttttc 4981 aagctgtaaa gaaaaacgtg tctgatgagg aaatgaaaaa aataatagat ttatacgatt 5041 atattgaagt aatgcctata gataataata gatttatgat tgataaagga gaagtaaagg 5101 atgaagaaga gttaagggag ttgaatagga aattaattga tacagccaaa aaatttgaca 5161 aaattccagt agctactggg gatgttcatt ttttagacaa acatgaagct gtcttaagaa 5221 aagttcttaa atattctcaa ggttttaaag tagatgaaga agaaacttat ttacatttta 5281 gaacaacaga tgaaatgttg gaagagttta gttatcttgg agaagaattg gcatatgaag 5341 ttgtagttga aaatagtaat ctgattgccg atatggttga agatataaaa cctataccag 5401 atgatactta tcctccaatt atagagggct cagatgtgga gttaagagaa atgtgttaca 5461 aaaaagccaa acgaatctat ggagacccaa taccagaaat agttcaaaaa agactggata 5521 gagaattaaa ttcaataata agcaatgggt atgcagttat gtatattata gctcaaaaac 5581 tggttgcaaa atctctttca gatggatatt tagttggttc aagaggctca gttggctcat 5641 cttttgcagc tactatgagt gatataacag aagttaatcc tcttccagca cattatattt 5701 gtgaaaacga agactgtaaa tattcattct tttatgaaat aggtgaatgg ggctcaggtg 5761 ttgatttacc agacaaggat tgtccaaagt gtggaagaaa acttgctaaa aacggtcatg 5821 atattccatt tgaggttttc ttaggttttg aaggtgataa ggaaccagat atagacttaa 5881 atttctcagg aaattatcag ccaacaattc ataaatacac agaagaacta tttggagagg 5941 ggtatgtata tagggctggg actataggta ctgtagcaga aaaaacagct tttggatacg 6001 caagaaaata tgtggaagaa aataatataa gtgtaccaaa tgcagaagta cttaggcttt 6061 caaatggatg tactggagta aaaagaacct caggacaaca tcctggtgga gttatggtta 6121 taccagacta taaagatgta tacgatttta cacctataca gtatccagca aatgatactt 6181 cttgtggtgt tataactact cactttgatt atcattcaat aagtggtaga atacttaaac 6241 ttgatatact tggacacgac ggtcctacca ttataaggat gcttgaagat atgactggaa 6301 taaatataac agaaattcct ttagatgata aagagactat gtctctattt acatctacag 6361 aagcattagg tgttacacca gaagaaataa actgtcctat aggttgcctt gctgtaccag 6421 agtttggaac taagtttgta cgacagatgt tacttgacac caaacccact acatttgcag 6481 aacttgtgcg tatatctggt ctctctcatg ggacagatgt ttgggtaaat aatgcacaag 6541 acttagttag agaagatata gttggattaa aagatgttat atctacacga gatgacataa 6601 tgaactattt aatatttaaa ggattacctc ccaaaatgag ttttacaata atggagagcg 6661 taagaaaagg taaaggtcta aaaccagaac acattgaaga aatgaaaaaa aataatgttc 6721 cagaatggta tataggctca tgtaaaaaga ttaagtacat gttcccaaaa gcacatgctg 6781 ttgcttatgt aatgacatca tttagaattg catattgtaa agttcactat ccagaagcat 6841 tttatgcgac ttattttacg acaaaagttg aggattttga tgcagattta attgttaaag 6901 gattagatgc tataaaatca aaaataaatg aaattgagtc actgggaaat gatgcaacca 6961 caaaagaaaa aggtatgttg accgttttag aagttgctct tgaaatgtat gctagaggaa 7021 taaaattatt accagttgat atatataaat cagatgcaac tgagtttata gttgtaggag 7081 aaaaaacatt acttccacca atggcagcaa tacaagggct tggaggaaat gcggctataa 7141 acatccaaaa tgaaagaaaa aatggtgaat ttatatcaaa agaagatttg agaaagagaa 7201 caaaaatatc aaaaacggta atagaaactc ttactaatca tggttcactt gagaatatga 7261 gtgagagaaa tcaattgtca ttgttttaag ttagcccaaa aagttgcaaa taaaagaatt 7321 ttatgatatc atagtataga aaatagtata ctttgcagaa actaagagtg agacagctgt 7381 tctcactctt ttattttaaa tatattatta aaaagttggt ttatttttat taaatatatt 7441 ttatttaaga ttatataaac taatcattta caattgaata ggaggaactt taatgaaaaa 7501 aaatttagaa gctacaattg aggagattgt aactaaaata acggatgaac atggatttga 7561 aatggttgat gttgaatatg taaaagaagc aggagaatac tacttaagag tatacataga 7621 taaagaagaa ggaatatctt taaatgaatg tgaattagtt agtagagaat taagtccaat 7681 actagatgaa aaagacccta taaaagaaaa ttacttttta gaggtatctt ctccaggact 7741 tgatagagca ttaaaaaaag acagagattt tgttagatat caaggtagag atgtagattt 7801 aaaattatat aaaccattaa atggatgtaa gcaatttgaa ggtgaacttg taggattaac 7861 agaagataat aatataaaaa tcattgcaaa tggtaaggaa atggaattta atagaaaaga 7921 tgttgctatt gtcagattag caataaagtt ttagtggtat attaaatagg gaggatataa 7981 aaaatgaatc atgaatttat ggaagcatta gatgaattag tagttgacag aggtatagat 8041 aaagaaatac ttatagatac tatagaacaa gctcttttaa ctgcatacaa gaaaaacttt 8101 ggttcagcac aaaatgttag agttgaattt gatagagaca agggagatat aaaagtattt 8161 tctcaaagag ttgttgttga tgaatcagat ttatatgata ctttcttaga aatagagtta 8221 gcggatgcta gagagataaa ccctaattat gaattaggtg atattataga acatgaagtt 8281 acacctaaaa actttggaag aattgcagct caaacagcaa agcaagtagt tgttcaaaga 8341 ataagggaag ctgagagaga aatagtgtat aatgaattta tagaaaaaga aaatgaaata 8401 gttacaggag aggttgctag agcaaataaa aatgttgttt atgtaaatct tggaagaata 8461 gaaggtataa tgactcaaag tgaacaaata ccatttgaga attataaagc aggtcaaaaa 8521 ataaaattct atatacttga agttaaaaag actaacaaag gacctcaaat agttgtatca 8581 agaagtcatc cgggtctagt aaagagattg tttgaagaag aagttcctga aatatatgaa 8641 ggaatagttc aaataaaatc tgtagcaaga gaagcagggt caagaactaa aatggcagta 8701 aaatctatag atgaaaaaat agacccaatt ggagcttgtg ttggaccaaa aggacttaga 8761 gtaaaaaata tagtggatga acttggcgat gagaaaatag atataataaa atatagtgaa 8821 gacccagcag agtttatatc agcagcactt agcccatcaa aagttgttag agtagatata 8881 gatgaagatg aaaagtctgc cttagtaatc gttccagatt atcagttatc gcttgctata 8941 ggtaaagaag gtcaaaatgc aagactagca gcaaaattga ctaactggaa aatagatatt 9001 aagagcgaat ctcaagcaga acaagaaatg ttaaatcttg aaaattccaa agaaatagaa 9061 gctgaggatg ttgaagctga atttatcgac caagaagagt taggtacttc agaggataat 9121 tttagtgaag aagagtaagg agggatgatt ttgaaaaaag taaaaaaagt gcctcaaaga 9181 aaatgcatag catgtcaaga tagagactct aaaaaagagc ttataagaat agttaaaaat 9241 aaagaaggaa aaatattttt agacccaaca ggaagagcca atggaagagg agcatatgta 9301 tgtaaaagct ctgaatgtct aaaaaaagct ataaagagta aagcattaaa taaagctttt 9361 aaaatagatg ttccagatga agtctatgat aatctactta tggagttaga agaatatgaa 9421 aaataataaa gaaaaagtac tttcgttctt agggctcgca actcgtgcag ggaaaatagt 9481 gtctggtgat gattcaactt tgcttgacct taaaaaagga aaagtaaatt tgattttaat 9541 cgcagaagat gcatcaaata atactaaaaa actttttaaa gataaatcaa cttttagaaa 9601 tattccatat ctattttttt caacaaaaga ggaaatagga tttgcaatag gaaaatctcc 9661 aagagcagtt gtaggaataa aagatgaaaa tttttctaaa aagataattg aactaataga 9721 aatttaaaat aaaatagggg gtgatcttgt gtcaaaaaca agagtatacc aaatagcaga 9781 agaattaaat atatcaaatg aagagcttat aaataagtta gctgaattag atataaatgt 9841 aactgacaaa gattcagtat tagaaggtga agagttagaa ttagcacttg aaatgttagg 9901 tgaagattta tctcaagaaa atggaaatgt tatagaaata gatggaaagt tgacagtaca 9961 agtattagca acgaaattag ataaatctcc atcagaaata attatgaagc ttatgaaaat 10021 gggaactatg gcaacaataa atcaagagat aagttttgag attgctgctt tggctgccaa 10081 agactatgga tttgaattaa cagtagctga aagtgatgat acagaagctt tagaaataga 10141 agctttaatg gaaattgaag aagataaaga agaggattta aaacctagac caccagttgt 10201 tacagttatg ggacatgttg accatggtaa gacatcttta ctagatgcaa taagaaaaac 10261 tgatgtgatt tcaggtgaag ctggaggaat aactcagcat ataggtgctt cagaagttaa 10321 aatcaatgga cacaaaatag tatttttaga tactccagga catgaggctt tcacatcaat 10381 gagagcaaga ggagcacaag ttacagatat agcaatatta gtagttgcag cagatgatgg 10441 tataatgcct caaacagttg aagctataaa ccatgctaaa gcagcaggtg tacctttaat 10501 agttgctatt aataaaatag ataaaccagg tgcaaaccca gataaggtta aacaggaatt 10561 agcagaccaa ggattattag ttgaagactg gggtggagaa gtaatagcag ttccagtatc 10621 agctaagaaa aaagaaggaa tagatacact tttagaaatg gtattattgg ttgctgaaat 10681 ggaagagtta agagctaatc caaataaaag agcagtagga actgttatag aagctgagct 10741 tgataaagga agagggccag ttgcaacagt acttgttcaa ggtggtactt taacagtagg 10801 tgacccaatc gttgcaggtg ttgcatgtgg taaagttaga gcaatgataa atgcaaaagg 10861 taagagagta aaaacagcag gaccatctac agcagttgag atattaggtc tatcagaagt 10921 tccacaagga ggagaccaat ttgtcgaagt tccaactgat aagattgcta gaagtgtagc 10981 agcaagaaga caacaaatag ttagagatga aatgttaaaa tcaactcaaa gattatcact 11041 agatgctcta tttagtcaaa tgagtgaagg ttctataaaa gaccttaata tagttataaa 11101 agcagatgta caaggttcag tacaggcagt taaacaatct ttagaaaaat tatcaaatga 11161 agaagttcaa gttaaagtaa tacatggtgg tgttggagct gtaacagaat cagacatatt 11221 acttgcagca gcttctaacg caataataat aggattcaat gtaagaccag ttccaggtgc 11281 agaatctcta ggagaaaaag aaaatgttga tataagaaca tatactataa tatacaaggc 11341 aatagaagat atacaagcgg ctatgactgg tatgttagac cctgaatatg ttgatgaaga 11401 aacaggaaaa gctgaaataa gagaaatata taaaatatct ggtgttggaa cagtagctgg 11461 atgttatgtg actaatggta agatatttag aaattgtaaa gtaagattag ttagagatag 11521 cataataata catgaaggtg agcttgcagc acttaagaga tttaaagatg atgtaaaaga 11581 agttaattct ggatacgaat gtggtatgag ttttgtaaat tataacgaca taaaagaagg 11641 cgatatagta gaagcttata taactaaaga agtagaaaga aaattataaa ttcgtatcaa 11701 agaaaatcag ttagtaaaga aggtcgatgg tatggcatca tataatagaa caagaaggat 11761 agcagaagaa ataagaaaag tcgtaagtac tatgcttata aatggagtaa aagaccctag 11821 aataacatct atggtgagtg taactgatgt agaagttaca aatgacctaa gatatgctta 11881 tgtatatgta agtattctag gtggagatga agaatccaca ctaacaggtc ttaaaagtgc 11941 tggaggatat ataagaagag aagtaggaaa aaatataaaa cttagatata ttcctgagat 12001 agtatttaaa cttgatgatt caatagaaaa aggaatgtat atggataacc ttataaaaag 12061 agttaatgaa aaaaatgctc aacaaaatga ggatgaaaat tatgatgaat aatatggata 12121 gtattataaa ctatatttat gagggtgact attttatagt tacctctcat atcagtccag 12181 atggagacaa tgttggttct acactttcaa tgtattatac attgaagaat ttaggaaaaa 12241 atgtatatta tgtacttgat gatgaagctc cattaaattt aaggtttttg gttgaaggtg 12301 taaaaatcta taagtctaat gaatttaata tgaagaacta ttctttaatt gcacttgatt 12361 gtggggataa gatgagaata tgtgtgagtg atgaaatcaa aaataatgca tcaaaattaa 12421 tctgtattga ccatcatgct agtaatgatt catatggaga tttaaattat gtagatattg 12481 atgcatcttc tacatgtgaa ctagtttata atttattggt tagatttcag caaacaaaag 12541 atattaatat tataaatgaa gatatagcaa catgtttata tacaggtctt gtaactgata 12601 ctggaaactt ttcgtattca aatgtacatg caagtagttt tgaaatggca aaaaatttac 12661 ttgttttagg tgcacaaaaa aatactataa tacaaaatat atatcaaagc aattcttctg 12721 attattataa attattagga gaagctctaa aaggtttgga gatatttgat tcaaaggtat 12781 ctagtatagt tttaactcaa gacatgatga atagaaataa tattagcttt aatgatgttg 12841 atggaataac aacatataca agagatataa aaggtataga agttggaatt ttatttaaag 12901 agaaaaaaca aaatgaaatt aaagtgagtt ttaggtcaaa aaattatgtt gatgtaagtg 12961 aaatagccaa gttatttggc ggtggtggtc atgtaagggc tgctggatgt actattagag 13021 atagtattga taatgcaaag aaaatggtac tagaggcagt gttaaaatca atttaaggtg 13081 acagtaaaat gaataagatt ataagtatat tgaaacccac aggtatgaca tctcatgatg 13141 ttgtaagtag agtgagaaag attttaaata taaaaaaagt tggtcataca ggtactcttg 13201 acccagatgc ttcaggtgtt ttaccaatct gtatagggaa agctacaaaa gtatgtgaag 13261 ttattttaaa caaagataaa tcgtatatat gtgagcttac tttaggtata agtacagata 13321 cttatgatgc ttcaggtgaa attttaaaaa aagttgatga ttttaaattt agtaatgaag 13381 acatagaaag agcttttgat acacaaagag gagagataaa tcaacttcct ccaatttatt 13441 cagctttaaa ggtaaatgga aagagaatgt gtgatttagt aagaagtggt agacaatctg 13501 aaattacttt aaaaacaaga agagtaaata taaaagacat aaaaatacta agtataaaag 13561 gtaataaagt aatgttttat gttgagtgtt ctaaagggac ttatgtgaga agtatttgcc 13621 atgatatagg agaatatctg ggttgtggag ctcatatgtc gtttttgaat agaacatctt 13681 ctggtaaatt tgacttagat aattcaataa cattagaaga attagaatta ttttatgaaa 13741 ataaaacttt agacaaatac ttatatgata tagattatgt ccttgattcg tttaattatg 13801 ttgtattaaa ccctaatgct ataaaatatt atagtaatgg tggctcaata gatgataaaa 13861 ggtttttaaa aaataatttc gataaagatg atgaagttgt aagagtatac agtacagata 13921 atttcttagg attaggaaag ttatcaaaac acaataatac aataagtgtt aaaagtgata 13981 agatgttcat ataaccaatg tatttaaaaa aacaacaaaa gagatagtac tataataact 14041 tctatagtct atctctttta cttatgctat aatagtttgt gaataagtta agagtaaatt 14101 atattttagc ggggaacatt tattatggtg attataaaat ctataaaaga aatagcaaac 14161 ataaaagaaa gtgtcattac cataggaaat tttgatggtg tacacaaagg acatcaagtt 14221 cttataggaa aaacagttga atatgcaaaa aaagaaaata tacagagtat tgtatttaca 14281 tttgcaaatc atcctgtaaa ttactttaga ccaaactcaa caaaaaatat tataagcaat 14341 gatgataaaa taaaattatt agaagatttg ggaatagata tagttgtaat cataccattt 14401 gatgagtata tgactaaaat acctgcaaag gatttcgtag atgagatact agtaaaaaaa 14461 ttaaaagcta aaaagatagt aattggacat gactttacat ttgcaagggc aaaagaaggc 14521 aatgttaatc tattaaaaag cttagaacat gaatttggtt ttgaggtaga agtaataaaa 14581 cccatcaaaa tcaatgatgt aagagtgagt agtacttata ttagaagttt agtatcccag 14641 ggtgatatgg ctaatgtcaa ggaatatttg ggtagaaatt acaagttaga aggttgtgtt 14701 atacattcta aacaccttgg aagaactata ggattcccaa cggcaaatat tgaccttaag 14761 aataatatgc tcgttccaaa gagaggtatt tatgcttcta ttgtacatat tggagatgag 14821 acttattttg gtgctacaaa tgtaggctat aacccaacag ttaatggaaa aactttgtct 14881 attgaaacaa atattttaga gtttgataga gatatttatg gggaaaatat aattgttgaa 14941 tttttggaaa gaataagaga tgaaagaaag tttaattcaa tagatgactt aaaaaatcaa 15001 ttgtataatg atacaaatta tgtgtatgaa aactatgttt gcaaaaacaa gtaatgtatg 15061 ataaaatgat taaggaaaat aaccattact cggcatttga atactccaat caatgcttag 15121 taataggcgt ttctaaaaaa atacggaggt aatctaaatg ataaacaaat cagaaataat 15181 aaaagaatat ggaagagctg aaggggatac tggttctcca gaagtacaaa tagctttatt 15241 aacagctaga ataaacgagt taaatggaca tttaaaaact cataagaaag accaccattc 15301 aagaagagga ctattaaaaa tggttggtag aagaagaaac ttattagctt acttaaagga 15361 aaaagatctt gaaggatata gagcattaat agctaaatta ggattaagaa agtaatttca 15421 ttctttataa aaaagcaggt atagtgtata cctgcttttt tatagttaat tcattataaa 15481 gtaaaatagt taataataaa agtagtttat atgttataaa aaattataaa atgaataata 15541 ttgggtaaga atattaaaat agtatacaaa tacataatca atttaaaagg aaagacagga 15601 taaatataga aattttataa caggataatt agttggaata aggaggatat tattttgaaa 15661 ataaagttag tatgcgatag tttgtgcgat attccagatg aaatatcaga aaaagattat 15721 atagagatag ttccacttac agttattttt aatgataggg aatatataga aggagtagat 15781 ataaagaaag aagagtttta taaaaaagta aaagaaatca agcaaatacc taaaacttca 15841 caagctacat atatggagtt taaagaagtg tttgataagt tcataacaga aggttatcat 15901 ataatctgta tgacaggggc tgcaaacgca tcaggaactt ttcaaagtgc tatgatagct 15961 aaaaatgatg taaatgaaaa tgaaaaaata catatatttg atactagaaa tttatctcta 16021 ggtagtggac aatatgttat aaaggcatgt gaacttcttg aagaaggact gggatttgaa 16081 gaaattatag atgaattaga aaatactcgc agtagtgtaa gattactttt tgcaccatat 16141 acacttgatt ttttaaagca gagtggaaga gttccagttg caactgcttt gataggaaat 16201 atgttaaata taaagcctat atttttcttt gataatgggg aagctaagtt ggtaaataag 16261 gttagaggaa taaagaatat agcaagtaaa ctggtcgata taattttaga aatgaatgaa 16321 gggtctttag aaggaaaaat cgtcacaata ggttgtggag ataatttaca cgattgtgaa 16381 atattaaaag atgaagtaaa taaaagaata aaagcaagaa gagtattatt tactcgagga 16441 ggagtttcta tttgctctca tacagggcca gatatattgg ctataagttg ttctaattag 16501 tagtaatata aggtttttgc atatatatgt gaagataatt ttatattgtt aatttatagt 16561 atttttgata atatagtaaa tagagtgata tacgtgtttt ttaataagag gaggacaagc 16621 attaatgttt gaacataaaa tatttaaaat ggattttgca ggaagagaac tttctgtaga 16681 aataggaaag atttgtgaaa tggctagtgg tagttgtata gttcgataca gtgacagtat 16741 ggtaatggta aatacaacta aatcagccaa acctagagac ggaatagact tttttccttt 16801 aagtgtggat tatgaagaaa aattatactc tgtaggtaag atacctggag gttttttaaa 16861 gagagaaggg aagccttcag aaaaagctat acttacatca agattgatag atagaccaat 16921 aagaccttta ttcccaaaag gatttagaaa tgatgttcag gtagttgcaa cagttctatc 16981 agtagaccaa gattgtacac cagatatagt agcaatgata ggttcttcaa tagccctttc 17041 tatatcagac ataccattta atggaccaac aggttcagta tgtgttggcc ttgtagatgg 17101 tgcatttgtt gtaaatccaa acgcagagca aagagaaaaa agttcaatgc atctagttgt 17161 ttcaggtaca aaggaagcaa taatgatggt tgaagccgga gctgatgaag ttccagatga 17221 ggtaatgctt gatgctattt tatttgctca ccaagaaata aaaaaaatag ttgagtttat 17281 agaaggtata gttgcagaag ttggaaaaga aaaaatgcct gttgaattat accatgcagg 17341 agaagaaata actcaattgg ttcgcgaatt tgcaactgat aagatgaaaa aagcagttca 17401 gacttttgaa aagttagaga gaatggaaaa tatggataga gttaaagaag aaactttagc 17461 tcactttgaa gaaactttag aagattttga agattttgtt ggagatatag aagaagtatt 17521 acaagatata ataaaagaag aagtaagaaa acttatagta catgaaaatg taagaccaga 17581 taatagaaaa cttgaagaaa taagacctat atggtgtgaa actggcatga tacctagagc 17641 tcatggttca gccatattta ctagaggaca aactcaagta cttaatgttg ctactttagg 17701 tgctttagga gatgttcaaa aattagatgg gttagatgaa gaagaaaaca agagatatat 17761 gcatcattat aatttccccg catatagtgt tggagaagct agaccatcaa gaggaccagg 17821 aagaagagaa ataggacatg gtgctttagc agaaagagct ttattaccag taataccatc 17881 tcaagaagaa tttccttatg ctattagatt agtttctgaa gtattaagtt caaatggttc 17941 tacatctcaa gcaagtgtat gtggctcaac attatcttta cttgatgcag gtgtacctat 18001 aaaagatatg gttgcaggta tagcaatggg acttataaag catgatggta aggttgcagt 18061 gctttcagat atacaaggta tggaagacca tttaggcgat atggacttta aagtagctgg 18121 aacagagtat ggtataacag caatacaaat ggatataaaa attgatggta tagataaaga 18181 aattctacaa cgtgctttaa aacaagctaa agagggtaga atccatatac taggagagat 18241 gagaaaaact atatctcagc caaaaccaga actttcacca tatgcaccta agattgttaa 18301 aatgcaaata aatcctgaca agataaaaga tgtaataggg cctggtggaa agataataac 18361 taagataata gacgaaactg gagttaagat agatattgaa caaactgggg aagtatttat 18421 atcaggtatt gagattgata tgattaagaa agctcaagaa cttataaata atatagtagt 18481 tgaaccagaa gtaggaaaaa cttataaagg taaggtaagt agaataatga actttggagc 18541 atttgttgaa atacttccag gaaaagaagg attacttcat atatctcata tagctcatga 18601 aagagttgcc aaagttgaag atgtattgaa tataggggat gaagttgaag ttaaagttac 18661 agagattgat gaaaaaggaa gagttaattt atcaagaaaa gttcttttac caaaaccaga 18721 acataaaaat aaataa //