LOCUS NC_015727:2 17777 bp DNA linear BCT 06-SEP-2013 DEFINITION Cupriavidus necator N-1 plasmid BB1p, complete sequence. ACCESSION NC_015727 SOURCE Cupriavidus necator N-1 plasmid BB1p, complete sequence. COMMENT locus start: 1050500; locus end: 1068276 COMMENT n1_4mer:GRV/n1_4mer:RV = 1.501324; n0_4mer:D = 24.075875; n0_4mer:PS = 24.259959 FEATURES Location/Qualifiers source 1..17777 /organism="Cupriavidus necator N-1 plasmid BB1p, complete sequence." gene complement(91..468) /gene="aldH" /locus_tag="aldH" /db_xref="1050590..1050967" CDS complement(91..468) /gene="aldH" /product="aldehyde dehydrogenase" /translation="VPEGSGDFFPVTLVDNPPDTGRVVCEEAFGPILPLIKFRDVDEV IARANNSEYGLGGSVWGRDEEAAMEVALRLNTGTVWVNQIHTIGPDKPMAGHKQSGVG IENDVDGLLEYTVPRTVSLRRSQ" gene complement(784..1146) /gene="" /locus_tag="" /db_xref="1051283..1051645" CDS complement(784..1146) /gene="" /product="LysR family transcriptional regulator" /translation="MPCRWDRLVLATTLSQPLAERSSMDFIDTLGYDFIGLPEASAIH NVLKRAAEDLQRELRWRVQVSNFETACRMIEANSALVCFRRIPSLAMRAQWRCGSSGS MMNGPNASCKSACPQPIV" gene complement(1152..1838) /gene="" /locus_tag="" /db_xref="1051651..1052337" CDS complement(1152..1838) /gene="" /product="transposase IS66" /translation="MVVAPGGWHDGAMLNLRQLQDQGLQDLAPEDVLALAQQMLAHIQ QQEREIKLKDVKIEKITFELARLKAWKFGAKTEAMSAEQRRLFEETLAEDEASLQAQL QQAKGEPSIEDNTKSKRTPRRQPLPAHLRRVEHYHEPEDTHCSTPGCGRTMVRVGEDV SEKLDIVPAEFFVHRHIYGKWACRCCQRLVQEPVEPQIIDGGLPAAGLVAHTLISRFV DHLPYYRQEA" gene complement(1873..2064) /gene="" /locus_tag="" /db_xref="1052372..1052563" CDS complement(1873..2064) /gene="" /product="hypothetical protein" /translation="MKLLVHDGFGVWCAARRLNQGRFTWPAIQAGATGAQLTQAQFDA LVLGLPWQRLEDLRAITRI" gene complement(2206..2538) /gene="" /locus_tag="" /db_xref="1052705..1053037" CDS complement(2206..2538) /gene="" /product="transposase IS66" /translation="MFLEQVRTPGASVAGVALSHGLNPNMVHPWMREDRQRQMLTALS EGHAFVPLQVDALSAVTGEVLSCKTPAAATPETIRMEIPRPGGTLVVHWPLSAAQQFS QLLRDWLR" gene 2479..3207 /gene="" /locus_tag="" /db_xref="1052978..1053706" CDS 2479..3207 /gene="" /product="hypothetical protein" /translation="VAERDPRNRSSWRANLFQEHRLEIGAVLPPARPLNCRHLIHRIH DPPSCGRCFIASIDSRWNNWSLTFYELHANHKSEVSGQALAYIGMLYDVEREVATLSP EERRRMRQEKAHAVADALHAWMIGQRQKVPDGSAIAKALDYSLKRWEALTRYLDDGNA PIDNNWVENQIRPWAIGRSNWLFAGSLRAGQRAAAVMSLVRSAQLNGLDPHAYLTDVL TRLPTQRASAIGELLPHRWTAAVS" gene complement(3339..4076) /gene="" /locus_tag="" /db_xref="1053838..1054575" CDS complement(3339..4076) /gene="" /product="LysR family transcriptional regulator" /translation="MLTSAGHELVRHAKSVLETVNEMSSAMSDFAVGVRGHVRVWANT SAVVQFLPNDLAAFLEREPLIRISLEEKLSYEVVEAVMRGVADIGVFADNVHAPGIQK FAYRKDVLVVLVPADHPLADRAEVQFADTLDFDFVGLNQGSSLLNRMTEAAAALERIL KLRIQVTSFDGICRMIEAGHGIGLLPAGAVREEILVAGLRAVRLADPWAVRSLWLGVR DLHALQPEANKLLQHLSGIELNAKTPQ" gene 4428..5741 /gene="dctA2" /locus_tag="dctA2" /db_xref="1054927..1056240" CDS 4428..5741 /gene="dctA2" /product="C4-dicarboxylate ABC transporter" /translation="MPTVDSAIPAGVPDERPHKKWYSHLYVQVLIAVAIGIIMGHIYP NAAQAMKPLGDNFLKLVKLMVAPLIFCTVVHGIASMRDIKAVGRLGGKALIYFEVMTT LALAIGLLVVNIMKPGVGMHVDPSTLDASSIATYTKAAHEQTVVGYLSHIIPHTVIGA FAEGDLLQVLFISIIFAFALQMLGERGKPMLDVIDSAANTFFNMVRIVMYVSPLGAFG AIAFTIGKYGIGSLASYGQLLVTYYLTAVLFVVIVLGAVCRLTGFGLFSFLRYIREEL LIVLGTSSSESALPRLMAKLERLGCDKSAVGLVVPSGYSFNLDGSCINMTVLAIFIAQ ATDTYLSFGHQVTLLAILLLTSKGAASVSGGAFIVLAATLGSIPGFPVAGLVLVLGVY RFISEGGAIVNVIGNGIATIVVAKWEKLLDEEALHLELQRGPDFN" gene 5812..6306 /gene="" /locus_tag="" /db_xref="1056311..1056805" CDS 5812..6306 /gene="" /product="" /translation="MDAKRVFIQEVATRDGFQNEAKYVETDEKVSLINALSECGYAKV EVTSFTSPKAIPALRDAEAVMHRISRHEGVVYTVLVPNVRGAERALSCSVDEVNLVMS VSESHNRTNLRMSREQSFAQLSDVIGLVRSTQTAINVYLAWAAAPTRPAPPETYAQKS SFTC" gene 6769..8082 /gene="dctA3" /locus_tag="dctA3" /db_xref="1057268..1058581" CDS 6769..8082 /gene="dctA3" /product="C4-dicarboxylate ABC transporter" /translation="MPNSVNQTDVLSTRPGSLFRFVARNLYVQVLLAMAIGITLGHYY PEIAAQMKPFGDGFIKLIKMTIGMVIFFTVVSGIAGMQSFGKVGKVGGIALLYFEIVS TIALLIGLVVANIIKPGESFNVDPAKLDASQIKQYVTAAQGNGLTDFFLNIIPKTLVG AFTSDSILSVVFVSILMGLVLGKIGDKGRPIRELVDACSHWIFGIINMIMRFAPIGAF GAMAFTVGKFGIASLQSLVVLISTFYLTAAIFVFVVLGAIGYWYGFSIFTVLRHFRSE LVLVLATSSSDAALPALMRKLENVGCKPGTVGLVMPAGYVFNSDGTNIYITMAVLFIA QAMHVELTIWHQLMILGVATLTTKGSAGVPGAGFVSLVATLALVPEIPLVGVTMLLGI DRIISEGRALVNVIGNVVATLVIARREGEVGSNDLQLAMRTVTSD" gene 8132..9199 /gene="mdh" /locus_tag="mdh" /db_xref="1058631..1059698" CDS 8132..9199 /gene="mdh" /product="malate dehydrogenase" /translation="MSDENDVLISECSLQRFCTQVLEKIGVPAVEALLATNNLIEADL RGVYSHGVVRFPIYVKRILDGGTNPKPKISVVRETRTTTVVDGDNGLGHLVGIRAMEM AIDKAKDGDCAFVAVKNSNHFGAAAYYAEMAARANMIGFSFTIGAINHMAPWGGAEAV LGNNPFAVAFPTREPFPIVLDMACSVAARGKIIVAAKEGKPIPGDWATGPDGLPTTDA VEALKGFVIPVGGPKGYALTLTVGLLSTMLSGAYFGSEVGDLYAKTTTAQNSGHLFGV LPVASFDDLDAYFDRIQRGIADIRGAKKATGVDRIYLPGEREYLSLVQHREHGIPIGP ALAEELSALAKAYHAEFDILT" gene 9217..9947 /gene="nanR1" /locus_tag="nanR1" /db_xref="1059716..1060446" gene 9999..10316 /gene="" /locus_tag="" /db_xref="1060498..1060815" CDS 9999..10316 /gene="" /product="hypothetical protein" /translation="VEDMLIICVTFTVYDAHIESFREAILANASTSLKEENGCLRFDV CQSKDGATFFFYEQYVDDQAFDVHLLSPHFLEFDRFSVPLVKDKKVERYYLVEQSACV TSS" gene 10413..11714 /gene="" /locus_tag="" /db_xref="1060912..1062213" CDS 10413..11714 /gene="" /product="hypothetical protein" /translation="LNSQGKKPLLGCIADDFTGATDLANNLVRAGMRVVQTIGIPTHT DIGSAIALETADAIVVALKSRTAPIDEAVEQSLSAANWLQSLGVAQLYFKVCSTFDST DRGNIGPVAEELAEVLQAGIVPVCPAFPETNRTVYKAHLYVGDVLLSDSSMRHHPLTP MTDSNLIHVLQRQSKGKVGHVDHAVVRGGSDTIRERLEALRTDGTRFAIVDAIANDDL MTMGRAFASMPLVVAGSGVAIGLPQNFGISPSESAAQLPRAQGPRAIVSGSCSAASNA QVVDFIKRGGEAFPVDPLRIAAGNDIVGEALHWAGSRLSESPVLFYATAAPEAVKSVQ AQLGVEHAGELVERTLSAIALGLVERGVRQLIVAGGETSGACVQALEVQQMRIGPQID PGVPWCYALSPAAGKDGLMLALKSGNFGTVDFFTKAFGMLP" gene 12589..13395 /gene="" /locus_tag="" /db_xref="1063088..1063894" CDS 12589..13395 /gene="" /product="LysR family transcriptional regulator" /translation="MAASAVSKRISDLEDALSARLLDRHSGGVRPTAAGLELVEYAKG VVDLLQRLRADMSLYSEGTKGDVRVFANSTSIVGFLDKDLPGFLLRYPQVEVRLEEWS SPYIVRSLKEGLADVGLFWADTPHAGLETEPYRSARLVVVVPADHPLAESLGVHFSET LEFAHVAFHEGSMIHRLTVQAAESFQRTIRTRLQVTSFDAMRTMVSAGVGLGIMTDVT VSAKPSLKELRVVPLLDEWAALDMRIGYRDRAALPRAAQQFVEHLLGNGG" gene 13503..14669 /gene="" /locus_tag="" /db_xref="1064002..1065168" CDS 13503..14669 /gene="" /product="L-carnitine dehydratase/bile acid-inducible protein F" /translation="MTLPLDGITVVALEQAIAAPLATRHLADWGARVIKVERPETGDF ARGYDKVMQGMSSQFVWTNRTKESVALDIKTEEGRRALDALLEKADVFLQNLAPGAAK KLGLDAKSLVERHPSLIACDISGYGSTGPFRHKKAYDLLVQCETGFLSINGTPEAQAK CGLSIVDIATGMYALNGVLMALYRRERTGKGTAFEVSLFDSMAEWMSYPAYYTRDSGT PLTRAGARHATIAPYGPFKAGDGKAVFFGIQNEREWQAFCGKVLEDAAIASDPRFSSN PQRLANQLELEALITARFAKWSSEEVAEKLETASIANARLNDVEAFLQHPQLHERDRI QSVSSPVGPLQVFKPPLIIDGIEPVLGPVPAVGEHTGKVLAELGLEASAQGSIQ" gene 14666..15574 /gene="mcl" /locus_tag="mcl" /db_xref="1065165..1066073" CDS 14666..15574 /gene="mcl" /product="malyl-CoA lyase Mcl" /translation="MNITRTYLAVPAHQEKLVTSAARSSADAVFLDLEDAVPESMKAT ALDAAVRAINDLDWGQKVIVVRVNGIGLRQQQEVESLVRLAPRLNAVLLPKVESAEEI VAVQRQVDGTSVAIEAMIETAKGLVFCEQIAAAGGGLEALHFGVGDFGASIGAKGVEI GLSHPQYALASRAGDGYADTALDMWAYPMMRILVAAHAFGLRAIDGPCGAFRDVRLSE SSALKAAAMGYDGKQVIHPLQLDCTNAAFTPTDEEYAAAQRVVAALAEAEREGRGAVQ LDGKLVDYANIRMAKKVVEARGRAIA" gene 15639..16610 /gene="bugT3" /locus_tag="bugT3" /db_xref="1066138..1067109" CDS 15639..16610 /gene="bugT3" /product="extra-cytoplasmic solute receptor BugT" /translation="MNRPVRMALACLLFGVSLAHAAASDTYPSKPVTIVVPQAPGGTN DIVARILGAALSQSTGQQFIVDNRPGAGGNIGSQYAARANPDGYTVLLTISSTQAINP ALYKKIPFDPVKDFDPVALVASVPNVLVAHPSFPASNMKDLIALAKSKPNELRYGSAG NGSLNHLLGEMLNNMAGISLEHVPYKGVGPALNDLLGNQIPLAFASLPSVLPHIKAGK LKALGVSSAKRSSAAPDIPAIAETIPNYSGDLWVALFAVHGTPKDVVSKLTTAVDKVL DDKSVQEKLKAQGAELPRATPQQLSGMLKSDMEKWAKIVKTSGATVD" gene 16824..17777 /gene="hprA" /locus_tag="hprA" /db_xref="1067323..1068276" CDS 16824..17777 /gene="hprA" /product="glycerate dehydrogenase HprA" /translation="MKIVFLDRSTISPQTTLKTLPFSHQFVFHDETAEEDVAGRIADA DVVITNKVKLSGQQLQQAPNLKLIAVAATGTDIVDLKACASRGIVVSNIRNYAVHTVP EHTFALIFALRRSLTAYHDSVRSGRWQQAGTFCYFDYPIKDLHGSVLGIIGDGVLGQS VAAIARALGIQPLFATYKGRQDMGPLYTPFEEVMRRSDIITLHCPLTPQTRDVIDTPE FEMMERRPLLINTARGGLVNESALVDALKSGKVGGAGFDVATQEPPGVDHPFHQLKDT PNFILTPHVAWASDEAVQGLADQLIDNIIAFARGTPRNVVV" ORIGIN 1 aagcctcggc gcaatggtcc aggcgagtat ggcgagctgg accacttgtg aggcgccacg 61 aaacctgagc cgccgccgcc aatttttttc ctattgcgat cgcctcagcg acaccgttct 121 gggcaccgta tattccaaga gaccatcgac atcgttctca atgccgacgc ccgattgctt 181 gtgaccagcc atgggcttgt cgggcccgat agtgtgaatc tggttaaccc agaccgtacc 241 ggtattaagc cgcagggcga cttccatagc ggcctcctcg tcgcgccccc agaccgaacc 301 acctaggccg tactcactgt tgttcgcgcg cgcaatgacc tcgtccacgt cgcggaactt 361 aatcagcggc aggatcgggc caaatgcttc ttcgcagact acccgtccgg tatccggcgg 421 attgtccaca agcgtgacag ggaagaaatc gccgcttccc tccggcactt caccgccgac 481 aagaatgcga tatccagcgt tgcgcgaccg ctctgcacat cctggagaac cccatgctca 541 acggcgagac catccgcctg gacggcgcca tccgcatggc gccacgctga gcggtcctat 601 cagtatgaga agcgctgcgc tgactgccgg agaacagctt gcctctgcgt gaaccatcag 661 tttctctgag agcggagacc atcagcactg cgcgactacc tgatgaatcg caggggccta 721 caagctcccc gagaagatcc tctgcttagt caccatctag cggcaagttg ctcaggcacc 781 agcttaaaca attggttgcg gacacgcaga tttgcaactt gcgttcggcc cattcatcat 841 tgagccggat gatccgcagc gccattgtgc ccgcatggcg agcgacggta ttctccggaa 901 gcacaccaat gccgaattgg cctcaatcat ccggcacgcg gtctcgaagt tgctgacctg 961 cacgcgccag cgcaactcac gctgcaaatc ctctgcggcg cgcttgagga cattgtgaat 1021 cgcgctggcc tccggtaggc ctatgaagtc gtagcccagc gtatcaatga agtccatgct 1081 gctgcgctcg gccagcggtt ggctcagcgt ggtagcaagc actaggcggt cccacctgca 1141 cggcatcacc tctaggcctc ctgccggtag tacggcaggt gatcaacgaa gcggctgatc 1201 agcgtgtgcg cgaccaagcc ggctgctggt aggccgccgt cgatgatttg cggttcaaca 1261 ggctcttgta ccaggcgttg gcagcatcgg caggcccact tgccatagat gtggcggtgc 1321 acgaagaact ccgccggcac gatatccaac ttctcgctga cgtcctcacc cacgcgtacc 1381 atggtccgtc cacagcccgg tgttgagcag tgagtatcct cgggctcgtg gtaatgctca 1441 actcggcgca ggtgcgccgg aaggggctgg cgacggggcg tgcgtttgga cttggtgttg 1501 tcttcaatag agggctcgcc cttggcctgc tgcaactgcg cttgcagact ggcctcgtct 1561 tcggccagtg tctcctcaaa caggcggcgc tgctcggcgc tcatcgcctc ggtcttggct 1621 ccgaacttcc aggccttgag tcgcgccagt tcgaaggtga tcttctcgat cttgacgtcc 1681 ttgagcttga tctcgcgttc ctgctgctgg atgtgtgcga gcatctgctg cgccagtgcc 1741 aggacgtcct cgggcgcgag atcctgaagg ccttggtctt gtagttggcg caggttcagc 1801 atggcaccat cgtgccagcc accgggcgct actaccatcc ggatttatgc tgtcaacgca 1861 ccgtgatcat cgtcatatcc gcgtaatggc gcggaggtcc tccaggcgct gccagggcag 1921 accgagcacc agggcgtcga actgcgcctg cgtcagttgt gcgccggtgg cgccggcctg 1981 tatggcaggc caggtaaagc gtccctggtt gaggcggcgc gcggcacacc atacgccaaa 2041 gccgtcatgc accaatagct tcatgcgcgt atggcgggcg ttcgagaaca agtaggcgtg 2101 atgagactga gctgcgccga agacctgcac cgcgcgccag caaccgctcg ctttcggcac 2161 gcatgtcgat gggttcgacg gccaaccaca gcgcgtcaat gcggatcatc gcagccagtc 2221 ccgaagcagt tgggaaaact gctgcgccgc tgacagaggc caatgcacaa ccagcgtgcc 2281 gcctgggcgc ggtatctcca tgcggatggt ctcgggcgtt gccgccgccg gcgtcttaca 2341 cgatagcacc tctcccgtga ctgcggacag ggcgtccacc tgtagcggaa caaacgcgtg 2401 gccctcgctc agcgccgtca gcatctgccg ctgacggtcc tcgcgcatcc atgggtgcac 2461 catattcggg ttcagcccgt ggctgagcgc gacccccgca accgaagctc ctggcgtgcg 2521 aacctgttcc aggaacatcg ccttgaaatc ggcgctgtac tcccgccggc gcggccgctc 2581 aactgtcgac acttgatcca tcgtatccac gatcctcctt cgtgtggacg atgcttcatc 2641 gcttcaatcg attcaagatg gaacaactgg tcgcttacgt tctacgaact gcacgccaac 2701 cataaaagcg aggtgtcggg tcaggcgctg gcgtacatcg gcatgctcta cgacgttgag 2761 cgcgaggtgg cgacactcag cccagaggag cgacgacgca tgcggcagga gaaggcgcac 2821 gccgtggccg atgcattgca tgcctggatg atcggccaac ggcaaaaagt gccagacgga 2881 tcggccatcg ccaaagcact ggattacagc ctcaaacgct gggaagcgct cacgcgctac 2941 ctcgacgatg gcaatgcgcc gatcgataac aactgggtgg aaaaccagat ccgaccttgg 3001 gcgatcggcc gctcgaactg gctgttcgcg ggctcattac gagcaggaca acgggcggcc 3061 gcggtcatga gcttggtgcg ttcggcacag ttgaacggac tggatccgca tgcgtacctg 3121 acggacgtgc tgaccagact gccgacccag cgcgctagcg ccatcggtga actgctgccg 3181 caccgttgga cggctgccgt ctcctgaaac catacaccgt caagaccgct tcgccagccg 3241 cctactttag ataggcaata tcatcctttc attacggcaa tgggaaaatg gccgtatgtt 3301 caaccaccct ttcccaactc gaaaacgtcg gcaagtcact actggggcgt cttagcattg 3361 agctcaatac cgctcaaatg ctggagaagc ttgtttgctt ctggttgaag agcatgcaaa 3421 tcgcgcacgc ctagccatag cgaacgcacg gcccaagggt ccgccagacg gacggcacgt 3481 agacctgcaa cgagtatttc ttcacgcact gcacccgcgg gtaggaggcc aatgccgtgc 3541 cccgcttcta tcatgcggca aatgccgtcg aaactagtca cctggatcct gagtttcaat 3601 attcgctcaa gcgccgccgc tgcttccgtc atccggttca aaagggagct gccctggttc 3661 agcccaacga agtcgaagtc caaagtgtca gcaaactgta cctcagctcg atctgcgagc 3721 ggatggtcgg caggcacaag gactactagc acatccttgc gataagcgaa cttttgtata 3781 cccggcgcat gtacgttgtc tgcgaacacc ccaatgtcag caacacctct catcaccgcc 3841 tcgaccacct cgtagctcag tttctcttcc aagctgattc ggatgagagg ctcgcgctca 3901 agaaaggcag ctaagtcatt gggcaggaac tgtactaccg cagatgtgtt ggcccacacc 3961 cgaacatgtc cgcggacccc aacggcaaag tcactcatag cgctcgacat ctcattcacg 4021 gtctccaaga cactcttggc gtgtcgcacc aactcatggc cggcagaagt gagcatcaag 4081 cccctaggga gcctaaaaaa caatgggcaa tccactgttc gctccagttc cgctatccgc 4141 ttgctaaccg ccgatagcgt catatgcgcc ttttccgacg cacgggttac gccgccgttc 4201 tctgcgacga gcagaaagat tcgaagggat tgaaggtcga aatgtgttgg cttaagcaca 4261 aaaattctct cgtattcgcg tccgctccta tagtaatcgc cctcagtagc ttttcccaga 4321 aggaaagggt tgttgaccga taactgattc tcggatgtag gcatctgtaa aaaaataccg 4381 ccctaacgag cggaattacc cgccgggcac agtagcagga gacagacatg cccacagtcg 4441 attcggccat tcccgccggc gtgccagacg aacgcccgca caagaagtgg tacagccacc 4501 tctatgtcca ggtgctcatt gccgtagcaa tcggcattat tatgggacac atctacccca 4561 atgccgccca agccatgaag ccgcttggcg acaacttcct gaagctggtg aagttgatgg 4621 tcgcaccgct aatcttctgc accgtcgtgc atggcatcgc aagcatgcgg gacataaagg 4681 cggtggggcg ccttggcggc aaagccctca tctacttcga ggtcatgaca acactggccc 4741 tggccatagg cttgctggtt gtcaacatca tgaagcccgg cgtcggaatg cacgtagacc 4801 catcgacgct tgatgcttcg tctattgcaa cctacaccaa ggccgcccat gagcaaacgg 4861 tggtcggcta cctctcccac atcattcctc acaccgtgat tggggcattt gcggaaggtg 4921 accttctgca ggtgttgttc atctccatca tctttgcgtt tgctctgcaa atgcttggtg 4981 aacgaggcaa gccgatgttg gacgtgatcg actcggctgc gaacaccttc ttcaacatgg 5041 tacgtatcgt catgtacgta tcgccattgg gtgccttcgg agcaatcgca ttcacgatcg 5101 gcaagtacgg cattgggtcg ttggcatcat atggccaact gcttgtcacg tattacctga 5161 ctgcggtgct gtttgtcgtg attgttttgg gtgcggtatg ccgcctaact ggtttcggct 5221 tgttcagctt cctccggtac atccgcgaag agctgctgat tgtccttggg acatcatcct 5281 cagagtctgc gcttcccagg ctgatggcaa agttggagcg cctcggttgc gacaagtcag 5341 cagtcggcct tgtcgttcca tcgggctatt ccttcaatct ggacggaagc tgcatcaaca 5401 tgaccgtcct tgcaatcttc atcgcgcagg caaccgacac atatctgtct ttcggccacc 5461 aagttaccct gctggccatt ctcctgttga cctcgaaggg cgcggcgagc gtgagtggcg 5521 gtgcattcat cgtactagct gccaccctcg gctcgatccc ggggttcccc gtcgcaggct 5581 tggtgctcgt gcttggcgtc taccgcttca tttcagaagg cggcgcaatc gtgaacgtca 5641 ttgggaatgg gatcgccacg attgtcgtcg ccaaatggga gaagcttctg gatgaagaag 5701 cactccacct tgagcttcag cgtggccccg atttcaattg agcagcaaga gctcaagttg 5761 ccgaagacag ggccggcttt cccaagctca ttcaagaagg aaaacaccat catggatgcc 5821 aagagggttt tcatccagga agttgcgacg cgcgacggat tccaaaacga ggcaaagtac 5881 gttgagactg atgaaaaagt aagtctcatc aacgcactga gcgagtgcgg ctatgcgaag 5941 gtcgaagtga catcctttac gtcacccaaa gccattccgg cccttcgaga cgccgaagcc 6001 gtgatgcacc gcatttcccg tcatgaaggc gttgtgtaca cggttctggt gcccaacgta 6061 cgtggcgcag aacgcgcgct ctcgtgtagc gttgatgagg tcaacctcgt catgtctgtc 6121 agcgagagcc acaatcgcac caacctcagg atgtcgcggg agcagtcatt cgcccagctc 6181 tcagacgtca tcggcctcgt ccgtagcaca cagacagcga tcaacgtcta cctggcttgg 6241 gcggctgccc ctacgcgccc ggcgccaccg gaaacgtatg cacagaagag ctcgttcaca 6301 tgctagagct tgatggatac gacacgggta tcgacctcgc tgccatcatg gctgcgtcag 6361 caaggcttcc tggactcatc gggcacgacg tcccgagtca actactcaag gccggtcgcc 6421 gtctagacct tcacccgatg cctcccaccg tcagcgtgcc ggagcagaat gcattcgcgt 6481 cctcggcagg ctgatccgaa actcacagcc tgcattcctc gcgcagcagg accgcgctct 6541 ccgaaacgag ccgcagcgtc cggccgccac attatcccgg gcagcggtcg tcgctgcccg 6601 cgcctctttt tgtcggccat ttagcagcgc cccagctcac acagaaccgc ttttccaaaa 6661 cgagggttta cccggccccg ctccagggtg tcagtcgttg cgctgtaggt agaccatacc 6721 tatggcgtct agggttgtct ggatgggaat tcaacggagc gagacgaaat gccaaattct 6781 gtgaaccaga cagacgtact atctacaagg ccgggaagtc tcttccgatt tgtggcgcga 6841 aacctctatg tgcaggttct gcttgccatg gcgatcggca ttaccttagg gcattactat 6901 cccgaaatcg ccgcccaaat gaagccattt ggcgacggat ttattaagtt gatcaagatg 6961 acaatcggta tggtcatctt cttcaccgtg gtttcaggca tcgccggcat gcaaagtttc 7021 ggcaaggtcg gaaaggtggg aggcattgca ctgctctatt tcgaaatcgt ctcgactatt 7081 gcccttctca ttggcttggt tgtcgcaaac atcatcaagc ccggcgaaag cttcaacgtc 7141 gaccccgcca agctggacgc gagccagatt aagcagtacg tcaccgcggc tcaaggcaac 7201 ggcttgaccg atttcttcct gaacattatt cccaagacgc ttgttggcgc attcaccagc 7261 gacagtatcc tttcggtggt gttcgtttcc attcttatgg gactggtgct cggaaaaatt 7321 ggcgacaaag gccgtcccat tcgcgaactg gttgacgcct gctcgcattg gatattcggc 7381 atcattaaca tgattatgcg cttcgctccg attggagcat tcggcgccat ggcattcacc 7441 gtcggcaagt ttggtattgc ctcactgcag tcactggttg ttctgatctc gactttctac 7501 ctgaccgcgg caatctttgt tttcgtggtc ttgggtgcga ttggctactg gtacggcttc 7561 agcatcttca cggttctgcg ccacttccgg agcgaactcg tgctggttct tgctacgtcc 7621 tcctcggatg ccgccctgcc ggccctcatg cgcaagctgg agaacgttgg ctgcaagcct 7681 ggaacggttg gcttggttat gcccgcgggt tatgtcttca actctgatgg aaccaacatc 7741 tacattacga tggcggtcct attcatcgca caagccatgc atgtggaact cacgatttgg 7801 catcaactca tgatcttggg cgtcgccacg ctgacgacca agggttccgc cggtgtacct 7861 ggtgccggat tcgtatcgct agtcgcaact ctcgcgttgg tgccggaaat cccactcgtc 7921 ggcgtaacga tgctgctcgg catcgaccgc attatcagcg aaggccgtgc actcgtgaac 7981 gtcatcggaa atgttgtcgc aacgctggta attgcgcgac gtgaaggtga agttggatca 8041 aacgacttgc agcttgctat gcgcaccgtc acttccgact gacatccgag tttcgagaac 8101 ctttccaatt catttcgaat gttgagataa tatgtctgac gaaaacgatg ttctgatttc 8161 ggagtgctca ctccagagat tttgcactca agtgctggaa aagatcggcg ttccggccgt 8221 tgaagcgctc cttgccacga acaacctcat cgaggcagac cttcgcggcg tgtactcgca 8281 cggcgtggtg cgtttcccca tctacgtaaa acgcatcctg gatggcggaa ccaacccgaa 8341 gccgaaaatt tcggtggtcc gcgaaactag gacgaccacc gttgttgacg gtgacaacgg 8401 cttgggccat ctggtgggca tccgggccat ggagatggca atcgacaaag ccaaggatgg 8461 cgattgcgcc tttgttgccg tcaaaaatag caaccatttt ggtgcggccg cctactacgc 8521 agagatggcc gcgcgcgcca acatgattgg cttcagcttc acgattggag ctattaatca 8581 catggctcct tggggcggcg ccgaagcggt actgggtaat aacccgttcg ccgtcgcctt 8641 cccaacgcga gagcccttcc cgattgtctt ggacatggcg tgcagcgtcg cagcgcgggg 8701 gaaaatcatt gtcgccgcaa aggaaggaaa acccattccg ggcgactggg caacgggtcc 8761 tgatggtctt cccaccaccg atgcggttga agcgctcaaa ggtttcgtca tccccgtggg 8821 cggcccgaag ggctacgctc tgactttgac agtcgggttg ctcagcacca tgctgtctgg 8881 ggcatatttt ggcagcgaag tgggcgacct gtatgccaag accactactg ctcaaaacag 8941 cggccacctg tttggtgtgc taccggtcgc gtctttcgat gacctcgacg catactttga 9001 ccggattcag aggggtattg ctgacattcg aggtgccaag aaagctactg gggtcgaccg 9061 catttatctt cctggcgagc gcgaatatct ttccctggtg cagcatcgcg aacatggcat 9121 tcccatcggg ccggccttgg ccgaagagct aagcgccctc gctaaggcgt atcatgcaga 9181 gtttgacata ctcacatgat aaagctgagg taaatcgtga aaggtgcact cctgccggtg 9241 gaaccggtgc gtcgccgcaa actgtccgag gaagttgccg agcaactgca gtcaatgatg 9301 cttagtggcc agctgcaggc cggccagtcg ctgccctcgg agcgtgagct aatgagcatg 9361 tttgcggtgg gacgttcctc ggtacgagaa gcactctatt actgcaacgg atgggcttag 9421 tctcattacg gaatgaggaa ccagcctcgg ttaccaggcc gtcagctcaa accctcgtgg 9481 acgagctctc tggagcggtg acgcatattc tggcagacgc ggaaggagcg cgggagctac 9541 agcaagcgcg cactttgttt gaggcgctgc tcgtgcgcta tgcggcggaa catgcatcga 9601 acgaggacat cgtcagactg aaggaggcgc tggacgccaa ccactcggca ataggcaatc 9661 aggctgagtt tgggaaaacg gacgtcgcat ttcaccaggt cctcgcagag ataccgaaga 9721 attcgatatt cacatcgctc caagctgccg ttgcggcctg gctggcggag cagcgattcg 9781 tatcgttgcg tatggagggg gcggaacagg cggcctatga cgcccaccgt gccatctacg 9841 aagcaatcgc tgcgcatgac cccaacgctg cggagcgagc aatgaactcg caccttaata 9901 aggtcggtga ctattactgg agcgcacgcc acgacaatag tgaatgaaca gtttttccga 9961 gttcattttg ctggaatcgc gcgactccac aactttttgt ggaagacatg cttataatct 10021 gcgttacctt cacagtttac gacgctcata ttgaatcttt tcgtgaggcg attctcgcta 10081 acgcgagcac ttcgctgaaa gaggaaaacg gctgccttcg gtttgacgta tgtcagagta 10141 aggatggagc caccttcttt ttttacgagc agtacgtcga cgaccaggcc ttcgacgtgc 10201 atctactcag ccctcacttc ctcgagttcg acaggttctc ggtgcctctg gtcaaggaca 10261 agaaggtcga acgctattac ctggtcgaac aatcagcttg cgtaacaagc tcgtgacacg 10321 ctggcccata tagggcctct gcattccaaa gtctgcgact gtgccatttc ccttggcaca 10381 gggccgcctc acgtacatac ggagcacgca agttgaattc acaaggcaag aaaccccttc 10441 tcggttgcat cgcggacgac ttcaccggcg caactgacct cgcaaacaat ttggtccgcg 10501 ccggtatgcg cgtggttcag acaatcggga ttccgactca cacagacatc gggtcggcaa 10561 tcgctttgga gactgcggac gcgatcgttg ttgccctgaa gtcacgcaca gctccgatcg 10621 acgaagccgt cgagcagtcg ctatccgctg caaattggct gcagagctta ggtgtggccc 10681 aactgtactt taaagtgtgc tcgaccttcg attcgactga ccgcgggaac attggccccg 10741 tggccgagga actggcagaa gttttgcagg cgggaatcgt cccggtctgc cccgcttttc 10801 cggagaccaa tcggacggtc tacaaggcac acctgtatgt cggcgatgta cttctgagcg 10861 acagcagcat gcgtcatcac ccgctcacac cgatgacgga ctcgaacctc attcacgtgc 10921 tgcaacgtca gtctaagggg aaagtcggcc atgtcgacca tgcagtagtt cgcggtggct 10981 ctgacacaat tcgagagcga ctggaagcgc tccgtaccga cgggactcga ttcgccatcg 11041 tcgacgccat cgccaatgac gacctcatga ccatgggaag agccttcgct agtatgccct 11101 tggtagtggc aggatccggc gtggcgattg gcttaccaca gaactttggc atttcaccca 11161 gcgagagcgc cgctcaactt ccccgcgctc agggcccgcg ggcgatagtg tcaggtagtt 11221 gctccgcggc gtccaacgcc caggtagtgg acttcattaa gcgaggcgga gaagcgtttc 11281 ccgtcgaccc gcttcgcatt gcggcaggca acgacatcgt cggcgaagct ctgcattggg 11341 ctgggtcccg actttccgaa tcacctgtgc tcttctatgc cactgccgcg cccgaagcgg 11401 tgaagtctgt acaggcgcaa ctcggcgttg aacatgcggg ggagcttgtt gagcgaacac 11461 tgtcggctat cgctcttggc ttggtggagc gcggcgttcg acagctcatc gttgccggcg 11521 gcgaaacttc cggtgcgtgc gtacaggctt tggaggtaca gcagatgcgt atcggcccgc 11581 aaatcgaccc cggggtaccc tggtgctacg ctctctcgcc ggcggctggt aaggatggcc 11641 ttatgctagc gctgaagtcc ggaaactttg gcaccgttga cttcttcacc aaagcctttg 11701 ggatgctgcc atgacggagc aggaacttcg gtttgagatt tgccgggtca gacgcagtct 11761 gtttgaacgc ggctacgtgc atgccaccgc cggtaacatc agcgtccggc ttgaagacgg 11821 ttacctgatc acaccaaccg atgcagcttg ggctttctcg agccggaacg gctggccaag 11881 gtcacgagca atggtgagca agtctcgggc gaccgagcaa gcaagacgct gacactgcat 11941 cgcagaatct atagcgcaga caccgaagca cgttgtgtcc tccacactca ttcaactcat 12001 ctggtggcac taactttatc gggagtctgg tctaccgaag acatcgtacc gcctatcact 12061 ccatacttcg tcatgaaggt cggccatgta cctttggttg agtatcaccg tccagggcac 12121 ccccgcgttg ccgatttggt ttccaaggca attgaagaac gccaggcacg gggagatcgg 12181 cttcgtgcgg tgacgctaga ccgattaggt ccgaacgtct ggggcgacac gccagcaagt 12241 gccatggcaa ccttggaaga gttggaggag actgcgccct tatggctgat ggctcatccg 12301 aagccggccc cctggatgaa gaggcaattg atgcgcttcg ccagaccttt ggggctgatt 12361 ggtaggaatg tcccgatatc gggggcggaa atcacatcaa gcgttccatg actgcgaaac 12421 ccgcccttac cgagcggcgc gtctagcaca ttcgcctcat gcgcgctatc ctgtccgcaa 12481 ttggcctacc tatcggcggc gattgtgcgg attgacccca ttaccctagc tatttttctc 12541 acggtcgttg aggagcagag tcttgcgcgc gccgcggagc gccatttcat ggccgcgtcc 12601 gctgtcagca agagaataag cgacctagag gacgctcttt ccgcgcggct ccttgaccga 12661 catagtggcg gcgtccgccc tacggcggca ggactcgaac ttgtggaata cgcaaaagga 12721 gttgtcgatt tactccagcg tctccgtgcg gatatgagtc tgtatagcga aggcacaaaa 12781 ggcgacgtgc gcgtcttcgc taatagcact tccatcgttg ggttcttgga taaggatctt 12841 ccaggatttc ttctccgcta tccgcaagtt gaagtgcgac tcgaggagtg gagtagtcca 12901 tatattgtcc gctccttaaa agaaggcctt gcggatgttg gactcttttg ggctgatacc 12961 ccacacgcgg gtctggagac ggagccctac cgatcggccc gcctcgtcgt tgtcgtgcct 13021 gcagaccacc cattagcgga gagcctagga gtccactttt cagagacgtt ggagtttgct 13081 catgtagcct ttcatgaggg cagcatgatt cacaggctga ccgttcaggc cgccgagtca 13141 ttccagcgaa ccatacggac taggctgcag gtcacgagct tcgacgccat gcgtaccatg 13201 gtatcggcgg gcgtggggct cggcatcatg accgatgtca ccgtttctgc aaagcccagc 13261 ctcaaggagc ttcgcgtcgt ccccttgctc gatgaatggg ctgcactcga tatgaggatt 13321 gggtaccgcg accgcgctgc attgcctcgt gcagcgcagc aatttgtcga acacctcctg 13381 gggaacggtg ggtagtactc agtcatcaaa atttggaacg gccgccatcg aaatccaatg 13441 atttacggcc gtcccagctt taccgacact agccattcaa gaaacaagac ggctggtgtc 13501 ccatgactct tcccctggat ggaattactg tagtagcgct ggagcaagcc atcgctgctc 13561 ccctggcaac acgtcacctc gcagactggg gggctcgggt catcaaggtc gagcgtcctg 13621 agaccgggga tttcgcacgc ggctatgaca aggtcatgca aggcatgtcg agccagtttg 13681 tgtggacgaa ccgcaccaag gaaagcgtcg cgctcgatat caagacggag gagggacggc 13741 gcgcgctgga tgctctcctt gaaaaagcgg atgtgttcct gcaaaacctg gcgcccggtg 13801 ccgccaagaa gctcggcctt gatgccaaga gcctcgtcga gcgtcacccc tccctgattg 13861 catgcgacat ctcgggctac ggctcaaccg ggccgttccg ccacaagaaa gcgtatgact 13921 tgctggtcca gtgtgaaact ggcttccttt ccatcaatgg gacacctgag gcacaagcca 13981 agtgtgggtt gtcgattgtc gacatcgcca ccgggatgta cgcactgaac ggcgtcctca 14041 tggcgctgta ccgacgcgag cgtaccggca agggcacggc attcgaagtc tcgctgttcg 14101 acagtatggc ggaatggatg agttaccccg cctactacac gcgtgacagc ggaacaccgc 14161 tgacccgcgc cggcgcgcgt cacgccacca ttgcgccgta cggacccttc aaggccggcg 14221 atggcaaggc tgtgttcttt ggcattcaga acgaacgcga gtggcaagct ttctgcggca 14281 aggttctcga agatgccgcc attgcttccg acccgcggtt tagctccaat ccgcaacgcc 14341 tcgccaacca actggagctg gaggctctga ttacggcacg ctttgcaaag tggtcgagcg 14401 aggaagtcgc ggaaaagctg gagaccgcct caattgccaa cgcgcgtcta aacgacgtag 14461 aagcgttctt gcaacaccca cagttgcacg aacgagaccg gattcaaagc gtttcaagcc 14521 cggtaggccc gcttcaggtg ttcaagcctc ctctcatcat cgatggaatc gagccagtac 14581 tgggaccggt tccggccgtc ggcgagcaca ccggcaaagt gcttgcagaa cttggcctgg 14641 aggcatcggc tcaagggagc atccaatgaa catcacgcga acctatcttg ccgtccccgc 14701 tcatcaggaa aagctggtga cgtcagctgc acgctccagt gcagacgccg tcttcctgga 14761 ccttgaggat gcagttccgg aaagcatgaa ggcgactgcc ctggatgctg ccgtccgtgc 14821 cattaatgac ctggattggg gtcagaaggt aatcgtggtc cgagtcaatg gcatcggcct 14881 gcgccagcag caggaagtcg agtcactggt tcgcttggcc ccccggctga atgccgtgct 14941 gttgccaaag gtcgagtccg ctgaggaaat cgttgccgta cagcgccagg tggacggcac 15001 cagcgttgcc atcgaggcga tgattgagac ggccaaggga ttggtcttct gcgaacagat 15061 tgcggccgcc ggcggcggcc tcgaagcact gcactttggg gtaggagact tcggcgcatc 15121 cattggtgcc aaaggggtgg aaatcgggct ttcccacccg cagtatgcgc tggcatcccg 15181 ggccggcgac ggctatgccg acactgccct tgatatgtgg gcttacccga tgatgcgcat 15241 cctggtcgcc gctcatgctt ttggcctccg ggctattgac ggcccgtgcg gtgctttccg 15301 tgatgtcagg ttaagcgagt cgagtgcgct gaaggcagca gccatgggct atgacggcaa 15361 gcaggtgatt cacccgctgc agttggactg taccaacgcg gccttcacac cgactgacga 15421 agaatacgcc gcagcccagc gagtcgttgc cgcccttgcc gaggcggagc gggaagggcg 15481 tggtgctgtg caactcgacg ggaagctcgt ggattacgca aacatcagga tggcaaagaa 15541 ggtcgtggaa gcacgcggcc gggcaattgc ttaaggcagt tcagcaccgg acatagccgc 15601 ccccatacga gggccggaat tacctcagga gacaaaaaat gaatcgaccg gttcggatgg 15661 cacttgcgtg tcttctcttt ggagtgtcac tggcacacgc agctgctagc gatacctatc 15721 cctcaaaacc tgtaaccatt gtcgtgcccc aggctccggg tggtacaaat gacatcgtcg 15781 ctcgcatctt gggcgcggcg ctttcgcagt ctacgggtca gcagttcatc gtcgacaacc 15841 ggcccggcgc cggcggcaac attggtagtc agtatgctgc gagagccaac ccggacggct 15901 acaccgtatt actgacaatc agtagcactc aggccatcaa tccggcgttg tacaagaaaa 15961 taccgttcga cccggtgaag gacttcgacc ccgtcgcgct cgtggccagc gtgcctaatg 16021 tgttggtcgc ccacccatcc ttcccggcgt cgaacatgaa ggacctcatc gccctggcga 16081 aaagcaagcc gaacgagctt cggtacggct cagccggcaa tggctcgcta aatcacttgc 16141 ttggtgaaat gctgaacaac atggccggca tttccttaga gcacgttccg tacaagggtg 16201 tgggtcctgc cctcaatgat ttgctgggca accagattcc gcttgcgttc gccagcctgc 16261 cgtccgtctt gccgcacatc aaggccggca agctcaaggc acttggcgtc agctccgcca 16321 agcggtcgag cgcggcgccg gacatccccg ccattgctga gaccattcca aactacagtg 16381 gcgacctgtg ggttgccctc ttcgcagtgc atggcacccc taaggacgta gtttccaagc 16441 tgacaactgc tgtggacaaa gtgcttgacg acaagagtgt ccaggaaaag cttaaggctc 16501 agggtgccga acttccccgc gccacgccac agcagctgtc cggcatgctg aagtctgaca 16561 tggagaagtg ggcgaagatc gtcaagacgt ccggtgcgac cgtcgactga ggtagtgtga 16621 ccggatggag gggaaactgg gcgccgacct tttccatttc ggaaaggaca gtcaactaag 16681 cctcgattcc cccgcagcag cgttccggtc atcatgtaag ccagcgcgaa gcagtcctgc 16741 ccgctcttcg ttcatccaat cccggcggga tggttcaacc atgcgtaaat cgacgcgtcc 16801 tgcccgcacc tggtgctatt cccatgaaga ttgtatttct ggaccggtca accatctccc 16861 cccagactac gctcaagacg ttgccgttct cgcatcaatt tgtttttcat gacgagactg 16921 ccgaagaaga tgttgccggc cgaattgccg acgctgacgt cgtgattacc aacaaggtaa 16981 aacttagtgg ccagcagctg caacaggcgc cgaatctgaa gctcatcgcc gttgcagcga 17041 ccgggaccga catcgtcgac ctgaaggcat gtgcgtcaag aggaatcgtg gtgtccaaca 17101 tccggaatta tgccgtgcac acggtcccgg aacatacgtt cgcactgata tttgcattgc 17161 gtcgaagctt gaccgcatac catgattctg tccgcagcgg acgttggcag caagcaggaa 17221 cgttctgcta cttcgattac cccattaaag accttcacgg ctccgtcctc ggcatcatcg 17281 gagatggtgt ccttgggcag tctgttgccg ctatagcccg tgcgctcggc atacagcctc 17341 ttttcgccac ctacaagggc cgacaggata tggggccgct gtacacgccc tttgaggaag 17401 tcatgcgtcg aagcgacatc atcacgttgc attgcccgct gacgccgcag acgcgcgatg 17461 tcatcgatac cccggaattc gagatgatgg aaaggcgccc cctcctcatc aacactgcgc 17521 gcggcggcct cgtcaatgaa tcagcgctcg tagacgcatt gaaaagcggc aaggttggcg 17581 gtgcgggatt tgacgtagcg acccaggaac cccccggtgt ggaccacccc ttccaccaat 17641 tgaaggacac acccaacttc attctgactc cgcatgtggc ctgggcaagc gacgaggccg 17701 tgcaaggctt ggctgaccag ctcatcgaca acatcattgc ctttgcacgg ggcactcccc 17761 gaaacgttgt ggtctaa //