LOCUS NC_010723:1 17599 bp DNA linear BCT 25-AUG-2011 DEFINITION Clostridium botulinum E3 str. Alaska E43, complete genome. ACCESSION NC_010723 SOURCE Clostridium botulinum E3 str. Alaska E43, complete genome. COMMENT locus start: 1; locus end: 17599 FEATURES Location/Qualifiers source 1..17599 /organism="Clostridium botulinum E3 str. Alaska E43, complete genome." gene 126..1496 /gene="dnaA" /locus_tag="dnaA" /db_xref="127..1497" CDS 126..1496 /gene="dnaA" /locus_tag="dnaA" /product="chromosomal replication initiator protein DnaA" /translation="MDADLNKLWEKTLNIVKSEMSEVSFNTWIKSCEPISISDTSIKI SVPNSFTKDILDKRYKSLVANSIEAVCSKLYDIKFIIESDLNNEDELNSSDNSIKNRN KNSTKNIVVNDEMSSTLNPKYTFNSFVIGNSNRFAHAASLAVAEAPAKAYNPLFIYGG VGLGKTHLMHAIGHYILQNNTKAKVVYVSSEKFTNELINAIKDDKNEEFRKKYRNVDV LLIDDIQFIAGKERTQEEFFHTFNELHDANKQIILSSDRPPKEIPTLEDRLRSRFEWG LIADIQVPDFETRMAILKKKADVENLKVANEVMGYIATKIKSNIRELEGALIRIIAYS SLTNREVTVDLASEALKDIISKKQGKHVTIPSIQEIVANYFNLKIDDLKSQRRTRNVA YPRQIAMYLSRKLTDMSLPKIGEEFGGRDHTTVIHAYEKISENLKSDENLQHTVSDIT KKVSQN" gene 1757..2857 /gene="dnaN" /locus_tag="dnaN" /db_xref="1758..2858" CDS 1757..2857 /gene="dnaN" /locus_tag="dnaN" /product="DNA polymerase III, beta subunit" /translation="MIFICEKHKLLDGISIVQKAITGKSTMKVLDGIYINATNDGLRL IGSDMDLSIQTLVKADVLDKGEIVIDAKIFGEIIRKLPNSDIKIETVKEDVIQITCEK SVFNVVCMNAEEFPSLPNINEDLKVEVNESILKNMIKGTSFSIAQDEARPILQGILFQ VKDRTLNLVALDGYRLAIRSEFLDNDINLEVVIPGKTLVEVSKILEDHDKNVDITFTN NHILFNLENTKIISRLLDGKFVNYTSLLPQEHKLLVTVKKEEFQNGIERASLMAKDGN NNLINLDLKEETLIITSNSQLGKVREEVLINLQGEEIEIAFNSRYLLDVLKNMESDEV ILEMTSGVSPCVIKEKDGENSQYLVLPVRQMR" gene 2896..3102 /gene="yaaA" /locus_tag="yaaA" /db_xref="2897..3103" CDS 2896..3102 /gene="yaaA" /locus_tag="yaaA" /product="S4 domain protein YaaA" /translation="MNKIKINTEIIKLDAFLKWSGIASLGSEAKIYIQEGLIKVNGEI CLQRGKKLKAGDIIEFEDEKFEIV" gene 3174..4259 /gene="" /locus_tag="" /db_xref="3175..4260" CDS 3174..4259 /gene="" /locus_tag="" /product="DNA replication and repair protein RecF" /translation="MYIKAIMLANYRNYNNLELNLSEGVNVFIGDNAQGKTNVLESIY YCAFAKSHRTSRDKDLINWKENEAYISLLVGKKRLDKRIDIKILRDGKKAIKVNSIKI NKIGELFGTFNVVMFSPEDLKIIKESPGIRRKFLDMELCQISKKYYFNLVQYNKILNE RNVILRSRDFNKDILEVYDLQLVECADYIVKERLEYIDKINYYGKFIHNEITSGKEDI VFKYDSGIKFKDNFKYAFLEKLRNNLLRDREQGITSVGPHRDDFNVLINNIDVKKFGS QGQQRTAVLTMKFSSLKIIKEITKEYPILLLDDVLSELDINRKRYVLSTLSDIQTIIT CTGINDLEDYLDDKSKVFNVCNGEIVN" gene 4274..4534 /gene="" /locus_tag="" /db_xref="4275..4535" CDS 4274..4534 /gene="" /locus_tag="" /product="hypothetical protein" /translation="VFLHLGENVVVPIKDIIGIFDLDNTMYSSDTIQFLRLAEEDGFV ERITNEKPKSFVIAEVNKMSKIYLSPISSATLTKRTDIEYNS" gene 4570..6477 /gene="gyrB" /locus_tag="gyrB" /db_xref="4571..6478" CDS 4570..6477 /gene="gyrB" /locus_tag="gyrB" /product="DNA gyrase, B subunit" /translation="LEQNNQKYDENQIQVLEGLEAVRKRPGMYIGSTSARGLHHLVYE IVDNSIDEALAGYCKNIKVKINKDNSITSSDDGRGMPVGMHPKMHKSAVEVIMTILHA GGKFGGGGYKVSGGLHGVGASVVNALSEQCIVTVKREGHIWQQEYSKGKVLYDLKQIG DTEESGTTIYFKPDAEIFDEVEFDFDTLSQRLRELAFLNKGINITLIDCRDDREENYY YEGGIKSFVAYLNRNKTPLHPEPIYVEGIKDKVTVELGLQYNDGYTENLFSFANNIDT IEGGTHLVGFKTALTRAFNDYAKRFGFIKENDKNFSGDDIREGLTAVISVKIEDPQFE GQTKTKLGNSEVKGIVDSIVSEYIGIFLEENPGTSKIIIDKALMAARAREAARKAREL TRKSVLERTTLPGKLADCSSKDPRECEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKI MNVEKQRLDKILNSETIRSMVTAFGGGIGKDFDIEKIRYNRIIIMTDADVDGAHIRTL LLTFFYRYMRELVEQGHVYIAQPPLFRVGKGKKETYAYSDAELDQVLQDMGGKDNSVD IQRYKGLGEMNATQLWDTTMDPSKRILLKAEIEDAMAADEIFTILMGEKVEPRREFIE QNAKNVVNLDI" gene 6500..9049 /gene="gyrA" /locus_tag="gyrA" /db_xref="6501..9050" CDS 6500..9049 /gene="gyrA" /locus_tag="gyrA" /product="DNA gyrase, A subunit" /translation="MEFNEGKIISVDIKNEMKKCYIDYAMSVIVGRALPDVRDGLKPV HRRILYSMQILGLSPEKGYRKCARIVGDVLGKYHPHGDTSVYDALVRMAQDFSMRYML VDGHGNFGSVDGDGAAAMRYTEAKMNKIAVEMLRDINKNTVDFMPNFDGEEEEPTVLP SRFPNLLVNGSSGIAVGMATNIPPHNLGEVIDGTIMLIDNPESSILELMTKIKGPDFP TGATIMGHAGIRSAYETGRGKIVVRAKSEIEEENGRHKIIFTEIPYQVNKAKLIENIA ELVKDKKITGISDLRDESDREGMRIVIELKRDANPNIILNLLYKHTKLQDSFGVIMLA LVNNETKVLNLKEVLVHYIDFQKEVITRRTTFELNKAEARAHILEGLKIALDNIDRVI SIIRNSSTSEIAKNTLMDEFNLSEKQSQAILEMKLRRLTGLERDKIDDEYNELLKQME YLRSILASEEKLLGVIKDELLEIKAKYGDERRTDIEQDMNEINIEDLIQEEDVVITLT KSGYIKRISADVYSAQRRGGRGIQAMSTKEDDFVDHITITSTHSDVLFFTNRGRVYKL RAYEIPDAGRQAKGTNIINLIAIEADEKIQTVLTVTDKRRDGYLFMGTKQGIVKKTHL NEFRNLRKNGLIAINLRENDELLKVKITYGDANVMFVTQNGYALRFNEKDVRHMGRTA SGVKAITLKDDDIAVCMDIAVDEEELLVISENGFGKRTPVSEYKIQHRGGVGLITYKI SEKTGKLVGATVCKVDDELMLINTNGVAIRINVSDISKTSRAAMGVTLMRTNEEEKIV AIAKILKSDDEDLEENEFESNEQREVVNEDSSLDELVERAEDNSENDTDIE" gene 9568..11074 /gene="rrsI" /locus_tag="rrsI" /db_xref="9569..11075" CDS 9568..11074 /gene="rrsI" /locus_tag="rrsI" /product="16S ribosomal RNA" /translation="ESLILAQDERWRRA*HMQVER*SFFGSGLAADG*VTRGQPAS*R GIAFRKED*YLIRL*FRMKRQ*KEQSAMRWARVALASW*GNGLPRRRCVADLRG*SAT LGLRHGPDSYGRQQWGILHNGGNPDAATPRE**RSSDCKTLSLGTIMTVPKEEATANY VPAAAVIRRWQALSGFTGRKGSVGGYLSGM*NTRA*PECCIPNWISRVQERKVEFLV* R*NA*ILGRTPVAKATFWTVTDTEARKRGERTGLDTLVVHAVNDEY*V*ELSWLLCRR *RIKYSAWGVRSQD*NSKELTGARTSSGACGLIRSNAKNLT*T*HLLNYS*SRKSLRR QEDRWCMVVVSSCREMLG*VPQRAQPLLLVATI*LSTLARLPWLTRRKVGMTSNHHAP YV*GYTRATMAGTKRCNTARWS*TIKPVSVRIVG*NSPT*SWSC**SRIRMSR*IRSR ALYTPPVTP*ELAIPKVRELTRKGGSDLR*GQRLG*SRNKVAVGEPAAGSPP" gene 11303..14212 /gene="rrlI" /locus_tag="rrlI" /db_xref="11304..14213" CDS 11303..14212 /gene="rrlI" /locus_tag="rrlI" /product="23S ribosomal RNA" /translation="GQATKGAW*MPWHQEPMKDAISCDKLRVDAHSQRSEDFRMRKLT WETPCIVK*IHSFMKVNPGN*NI*VPGGRERKIDFLSSGERKGKSPNQRFIFGVADRT LTRNYG*PNTTGKLDRRG**SCR*KL**SSSDPEYHETRETLWEAGRTTSQG*ILPDD R**SSTVRER*KEPREGSEIEPETMCLQPIKAPYVCDDVLFVERANELRYVARLST*G TEPKGNRVLIGRLVACCRPETG*PIHGQVEARVKPRGGPNHVAVEKAWDELWIAEKFQ SNSDIAGSPRNSFRASVGKCEYWG*STEWARGHSAYRTLSNSEYQILSVRQSDCER*V P*SKGKQPRSSAKVPKCKLSGKGCGISKTTRMLA*KQPLIKRVRNSSLVKRSCAENVR GSNLPPKLRVHTIV*AVEERRNRAEVVP*GAVD*LRVRMLALVARCG*ESHRPNT*GF LRKVRPLRVSRDLS*GRKA*AMDNRLIFRYHYYRYYRWCDGEG*DVLAIGC*SKRLGS *NRQIRSNNSEA*WGRF*GTEVSDSMLPRKASRKRNSARTANRHR*VRRES*GRRKNC S*GTRQIDPVTSGEGVPARAGRRE*AQATV*QKHRSLLKRKLMYRG*RLPGAGRLRGT LSVSEGVNLSPSKRRP*L*RS*GSEIPCQVSSDPHEWRNDLCTVSTANPAKL*CE*RC SLPAIGRKDPVELYCSLALNFGIVCTG*VGDWEIRASALMESSLGYHPDSTGILTGCH ESGDRTLLGGQFDWGGRLLKSNGGAQRFPQNGRKSFVECKGIREPDCETYKSSRDESR A**SGGTSWEGHRSTDKSYLGDNRLISPKSSHRRGGLAPRCRLVASWG*SRSQGLGCS PIKAARELGSERRETVRSLSVAGAGNLRRAVLSTRGPGWTDLWCTSCFARSIAG*LSR DGINAESI*A*SLLQDEISHSVS**DPLKTTRLIGQRCKCGNIFS*LILIGRGLD" gene 14272..14388 /gene="rrfI" /locus_tag="rrfI" /db_xref="14273..14389" CDS 14272..14388 /gene="rrfI" /locus_tag="rrfI" /product="5S ribosomal RNA" /translation="SGDDGIEVTLLSIPNRTVKIYSAEGTAWETVWEIRTLP" gene 14392..14468 /gene="" /locus_tag="" /db_xref="14393..14469" CDS 14392..14468 /gene="" /locus_tag="" /product="tRNA-Met" /translation="GSLAQLVRATGS*PVGPGFESLKVH" gene 14472..14549 /gene="" /locus_tag="" /db_xref="14473..14550" CDS 14472..14549 /gene="" /locus_tag="" /product="tRNA-Ala" /translation="WGYSSVGRAPALHAGGQEFESLYLH" gene 14632..15135 /gene="" /locus_tag="" /db_xref="14633..15136" CDS 14632..15135 /gene="" /locus_tag="" /product="putative HD domain" /translation="MLYRVKQFILGILSYFESDNVNFINKYLNKKEKNLFMKLSKPDR IHSLRVCKEAILIGKDYDTVDEFKIAKCALLHDIGKVEISLNIIEKGVVVIINKVTKR SFLKYNKYSRMTKYYNHPAIGKELLEKINFEDKEILYCIENHHKNLDEIEENLYLKIL NICDSKN" gene 15174..15686 /gene="" /locus_tag="" /db_xref="15175..15687" CDS 15174..15686 /gene="" /locus_tag="" /product="3H domain protein" /translation="VNSIERRESILKSLIQSKTPLKGVEIANKYGVTRQIIVKDIAIL RAKGNNIMATPDGYIINKELNKIKAIIAVNHKEEELVHEMSIVIKYGGTIEDVIVEHP LYGEIKGMLMIKNYNELNKFAEKYKKQQGRLLSVLTNGVHIHTISADTQEDINSIIDE LKENGFIVSD" gene 15699..17090 /gene="" /locus_tag="" /db_xref="15700..17091" CDS 15699..17090 /gene="" /locus_tag="" /product="FAD-dependent oxidoreductase" /translation="MDYDVLILGGGIVGCAVAYQLSKYNINIALIEKDYDVADDISFI NTAVIYDGSETSNDMMAKLEGIGRKLIEEHCKKFNVSYEKTGALRITSNNNQEYKLDI MYDRAKKRNIEGIHLIEDDKELLHKIEPNLNIDVNKAFYSENVAVISPYDLAISYAEV AADNGVNFKLEEEVIDIQSLARGFKVTTNKNKFTCKFVVNTIPDEISFNNTEEEYDYK NMNYIMFENKSEDKLKTILIDEIDDTTFVINNPKLQNGNLIGIKSNHILSLEEGIDMS VSIFPEAKKDFVSNMFAEVYNKDIMIIDDSKLNSGYIKITGTHYGKITIAPAIALKIK NTLKDNLNIIRKKDYIDKRREVYRFNDLSNKERNEVISVDKRYGNIICVCNNISEGEI IDSIRRPLGARTVEGVKRRTGIGRGNCNGSYCDIKIIKILAREMDKNILDIVEDSKDS NIVSSRIKEFKEI" gene 17109..17345 /gene="" /locus_tag="" /db_xref="17110..17346" CDS 17109..17345 /gene="" /locus_tag="" /product="hypothetical protein" /translation="MSSKDIFTSLVRVKGSPNHKVVSVKSNNPIEKELWREFSKVLSR IYVSAPINIGDIICKNIMNTGIDIVCTKKIDNNK" ORIGIN 1 atgtgtataa gtttattcgc ttagtcaaca attaaaatat attgtttatt atattgttaa 61 tatcttgtta ataatttata tatacactaa aaaaaatttt tttttaataa tatttggagg 121 atagagatgg atgctgacct aaataaatta tgggaaaaaa ccttaaatat agtaaaaagt 181 gaaatgagtg aagtcagttt taacacttgg attaaaagtt gtgaacctat ttctatatct 241 gatacaagta taaaaataag cgttccaaat tcttttacta aagatatttt agacaaaaga 301 tacaaaagtt tggttgctaa ttctatagaa gctgtttgtt ctaaattata tgatataaaa 361 ttcataatag aatcagatct taataatgaa gatgaattaa atagtagtga taattccatt 421 aaaaatagga ataaaaattc tacaaaaaat atagttgtga atgatgaaat gtcttcaact 481 ttaaacccta aatatacttt taattcattt gtaataggta atagcaatag atttgcccat 541 gcagcttcac ttgccgttgc tgaagcgcct gctaaagcgt ataatccttt atttatatat 601 ggaggcgttg gtcttggaaa gacacattta atgcatgcaa ttggacatta tattttacaa 661 aataatacta aagctaaagt agtttatgtt tcttcagaaa aatttacaaa cgaattaata 721 aatgctatta aagatgataa aaatgaagaa tttagaaaaa aatatagaaa cgtagatgtg 781 ttgcttatag atgatattca attcattgca ggaaaagaac gtactcaaga agagttcttc 841 catactttca atgaactcca tgacgcaaat aaacaaataa ttctatcatc tgatcgtcca 901 ccaaaagaga ttccaacatt agaagataga ttaagatcaa gattcgaatg gggtttaatt 961 gctgatattc aagtacctga ttttgaaact agaatggcaa ttcttaagaa aaaagccgat 1021 gtagaaaatt taaaagtagc aaatgaagtt atgggataca tcgctacaaa aattaaatct 1081 aatattagag aattagaagg tgcattaata agaataattg catattcatc tttaactaat 1141 agggaagtta cagttgattt agcatcagaa gcactaaaag atataatctc taaaaaacaa 1201 ggaaaacacg taactatacc ttctattcaa gaaatagtag ctaactattt taatttaaaa 1261 atagatgatt taaaatctca aagaagaact agaaatgtag cttatccaag acaaatagct 1321 atgtatttaa gtcgaaaact aacagatatg tctttgccta aaataggaga agaatttggt 1381 ggtagagatc atactactgt aatacatgct tatgaaaaaa tatctgagaa tctaaagagt 1441 gatgagaact tacaacatac tgtttctgac attacaaaga aggtttctca aaattaatta 1501 acatatgtac ataataagta ttgaataact tgtggataac attagtttaa taatattcta 1561 ttgaaaactg tggataagat gtgctttttg ttactgactt atccacaatc tttttgcaca 1621 aaattccgtt gatattacta tgtttgaagt acttatcaac aaatcaacag cccctactac 1681 tattactatt atatataatt atatatctat tctatttaaa ttaaaaaata tatgtgaata 1741 attaaggagg attttttatg atctttatat gtgaaaaaca taaattatta gatggtattt 1801 ctatagttca aaaagctatt acaggaaagt caacaatgaa agtattagat ggtatttaca 1861 taaatgctac taatgatgga ttaagactaa taggttcaga catggatctt agtattcaaa 1921 cattggttaa agcagatgtt ttagataaag gtgaaatagt tattgatgct aagatatttg 1981 gagagattat tagaaaactt ccaaactcgg atataaaaat agaaactgta aaagaagatg 2041 taatacaaat tacttgtgaa aaatcagttt ttaatgttgt atgcatgaat gctgaggaat 2101 ttccatcatt acctaacatt aacgaagact taaaagtaga agttaatgaa agtattttaa 2161 aaaatatgat taagggaaca tctttttcta ttgcacaaga tgaagcaaga cctattttac 2221 aaggaatatt attccaagtt aaagatagaa ctttaaattt agttgcactt gatggatata 2281 gattagctat aagaagcgaa tttttagata atgatattaa tttagaagtg gttattcctg 2341 gaaagacatt agttgaggtc tcaaaaatat tagaagacca tgataaaaat gtggatataa 2401 cttttactaa taatcatata ttatttaatt tggaaaatac taaaattata tcaagactat 2461 tagatggtaa atttgtaaac tatacatcat tactacctca agaacataaa ttattagtca 2521 ctgtgaaaaa agaagaattt caaaatggta ttgaaagagc ttctttaatg gccaaagatg 2581 gaaataataa tttaatcaat ttagacctta aagaagagac gttaataata acttctaatt 2641 ctcaattggg aaaagttagg gaagaagtat tgataaatct gcaaggtgaa gaaatagaaa 2701 ttgcatttaa ttcaagatat ttattagatg ttcttaaaaa tatggagagt gatgaagtga 2761 ttttagaaat gacatcaggg gtaagtccat gtgttataaa agaaaaagat ggggaaaatt 2821 cccaatattt agttctacca gtaagacaaa tgagataagg aggaaataaa tatttattaa 2881 tatttatgca aaaaatatga ataaaattaa gattaatact gaaattataa aattagatgc 2941 gtttttgaaa tggagtggta tagcttctct aggatcagaa gctaagattt atatacaaga 3001 aggtttaata aaagttaatg gagaaatatg cttacaaaga ggaaagaaat taaaagctgg 3061 agatattata gaatttgaag atgaaaaatt tgaaatagtt tagtatagct tttaattttt 3121 aataaatagg cgataacttt tctattatga tataattgat tataggtgta ttttatgtat 3181 ataaaagcga taatgttagc taattataga aattacaata atttggagtt aaatcttagt 3241 gaaggtgtta atgtatttat aggtgataat gcccaaggta agactaatgt tttagaatca 3301 atttactatt gtgcatttgc taaatctcac agaacatcac gagataaaga tttgataaat 3361 tggaaagaaa atgaagcata tataagttta cttgtaggaa aaaaaagact agacaaaaga 3421 atcgatataa aaattttaag agacggaaaa aaagctataa aggttaattc tataaaaata 3481 aataaaatag gagaactttt tgggactttt aacgtagtta tgttttcacc agaagattta 3541 aaaataataa aagaatctcc cggaataaga agaaaatttc tagatatgga attatgccaa 3601 ataagtaaaa aatattattt taatctagtg cagtataata aaatattgaa tgaaagaaat 3661 gtaattttaa gatcaagaga ttttaataaa gatattttag aggtatatga tttgcaatta 3721 gtagaatgtg ctgattatat tgttaaagaa aggttagaat atatagataa gataaattat 3781 tatggaaaat ttattcataa tgaaataact tcaggaaaag aagacatagt tttcaaatat 3841 gattcaggta taaagtttaa agataatttc aaatatgctt ttttggaaaa attaagaaat 3901 aatttattaa gagacaggga acaaggaata acatcagtag gtccacatag agatgacttt 3961 aatgtattaa taaataatat agacgtgaaa aaatttggtt ctcaaggtca acaaaggact 4021 gcagttttaa ctatgaaatt ttcttcactt aagattatta aagaaattac aaaggaatat 4081 ccaattttat tacttgacga tgttctttca gaactagaca taaatagaaa gcgatatgta 4141 ttaagtactc ttagtgatat acaaacaata attacttgta cgggtataaa tgatttagaa 4201 gattacttag atgataaatc taaagtattc aacgtatgca atggagaaat agtgaattaa 4261 aggaggaata tatagtgttt ttgcatttag gagaaaatgt tgtagttcct ataaaagata 4321 ttattggaat attcgattta gataatacta tgtatagttc agatactata caatttttaa 4381 gattagctga agaagatggc tttgtagaaa gaattacgaa tgaaaaacct aaatcttttg 4441 ttattgctga agttaataaa atgagtaaga tatatctttc gccaatatct tctgcaacat 4501 taactaaaag aactgacata gaatacaatt cgtaagctat aaatattttt aaatttagga 4561 ggagtctgtt ttggaacaaa ataatcaaaa atatgatgaa aatcagattc aagtacttga 4621 agggttagaa gctgttagaa aaagaccggg gatgtacata ggtagtacta gtgctagagg 4681 actacatcac ttagtatatg aaatagttga taatagtata gatgaggctt tagcaggata 4741 ttgtaaaaat ataaaagtta aaatcaataa ggataattct attacttcat ctgatgatgg 4801 aagaggaatg cctgttggta tgcatcctaa aatgcataaa tcagcagttg aagtaataat 4861 gactatactt catgccggcg gaaaatttgg tggtggagga tacaaagtat ctggtggttt 4921 acatggtgtt ggtgcttctg ttgttaatgc tctttcagaa cagtgtattg ttacagtaaa 4981 aagagaaggt catatatggc aacaagagta tagtaaaggt aaagtacttt atgatttaaa 5041 acaaattgga gacactgaag aaagtggtac aacaatatat tttaaacctg atgcagaaat 5101 atttgatgaa gttgaatttg acttcgacac attatctcaa agattaagag aattagcttt 5161 tttaaataaa ggcattaata taacattaat tgattgtaga gatgataggg aagaaaatta 5221 ttactacgag ggcggaataa aatcctttgt agcttatttg aatagaaata agacaccact 5281 acatccagaa cctatatatg tagaaggaat aaaggataaa gttacggttg aattaggatt 5341 acaatataat gatgggtata cagaaaattt attttctttt gctaataaca ttgacacaat 5401 tgaaggggga acccatttag ttggattcaa aactgcatta actagagctt tcaatgatta 5461 tgcaaaaaga tttggattta taaaggaaaa tgataagaac ttttctggtg atgatataag 5521 agaaggtctt acagcagtaa tatctgttaa aatcgaagat cctcaatttg aaggtcaaac 5581 taaaacaaaa ttaggtaata gtgaagttaa gggaattgtt gactctatag ttagtgagta 5641 tataggaata ttcttagaag aaaatcctgg aactagtaag ataattatag ataaagcttt 5701 aatggcagca agagcaagag aggctgctag aaaagcaaga gaattaacaa gaaaatctgt 5761 gctagaaaga acaacattac ctggtaaatt agcagattgt tcatctaaag atcctagaga 5821 atgtgagatt tatatagtcg aaggggattc agccggtgga tctgcaaaac aaggtaggga 5881 cagaaaattc caagctattt tacctttaag aggtaaaata atgaatgttg aaaaacaaag 5941 attagataaa atattaaatt cagaaactat aagatcaatg gttactgcat ttggtggtgg 6001 aattggtaaa gattttgata tcgaaaaaat tagatataat agaataataa ttatgactga 6061 tgccgatgtt gatggtgcgc atataagaac attattatta acatttttct atagatatat 6121 gagagaacta gtagaacaag gtcatgtata cattgcgcaa ccgcctttat ttagagttgg 6181 taaaggtaaa aaagaaacat atgcttattc tgatgctgaa ttagatcaag tattacaaga 6241 tatgggtgga aaagataatt cagtagatat tcaaagatat aaaggtttag gagaaatgaa 6301 tgctactcaa ttatgggata caactatgga tccatcaaag agaattctat taaaggctga 6361 aattgaagat gcaatggcag ctgatgagat atttactatc ttaatgggag agaaagttga 6421 accaagaaga gaatttatag aacaaaatgc taagaatgtt gttaatttag acatttagta 6481 cttaatatga ggtgaagtac atggaattta atgaaggaaa aattatatct gtagatataa 6541 aaaacgaaat gaaaaaatgc tatatagact atgctatgag tgttatagta ggtcgtgcat 6601 taccagatgt tagagatggg ttaaaacctg ttcatagaag gatattatat tctatgcaaa 6661 ttctaggatt atcacctgaa aagggatata gaaaatgtgc tagaatagtt ggagacgttt 6721 taggtaagta tcatccacat ggagacactt ctgtttatga tgcattagtt agaatggctc 6781 aagatttctc aatgagatat atgttagtag atggacatgg aaactttggt tctgttgacg 6841 gtgatggtgc tgcagcaatg agatatacag aagctaagat gaataaaata gcagttgaaa 6901 tgttaagaga tattaacaaa aatactgttg attttatgcc aaattttgat ggtgaagaag 6961 aagaaccaac agttttacca tctagatttc caaatctttt agttaatgga tcatcaggaa 7021 tagcagttgg aatggcaact aatatacctc ctcataattt aggggaagta atagatggaa 7081 caataatgct tatagataat ccagaatcta gcatactaga gcttatgact aaaattaaag 7141 gtcctgattt cccgactggg gcaacaataa tgggtcatgc aggaattaga tcagcttatg 7201 aaactggaag aggtaaaata gtagttagag ctaaatcaga gattgaagaa gaaaatggta 7261 gacataaaat aatatttact gaaatacctt atcaagttaa taaagctaaa cttatagaaa 7321 atattgctga attagtaaaa gataaaaaga ttactggaat atctgattta agagatgaat 7381 cagatagaga aggtatgaga atagtaattg aattaaagag agatgctaat ccaaacataa 7441 tattaaactt attatataag catacaaaac ttcaagatag ttttggtgtt attatgttag 7501 cattagtaaa taacgaaaca aaggttttaa acctaaaaga agtattagtt cattatatag 7561 acttccaaaa agaagttata acaagaagaa ctacttttga gctaaataag gcagaagcta 7621 gagcacacat attagaaggt ttaaaaattg cactagataa tattgataga gtaataagta 7681 taataagaaa ttcatctaca agtgaaatag ctaaaaatac tttaatggat gaatttaatc 7741 tttcagaaaa gcaatctcaa gcaattttag aaatgaaatt aagaagatta acaggcttag 7801 aaagagataa gattgatgat gaatataatg aattattaaa acaaatggaa tatttaagat 7861 ctatattagc aagtgaagaa aaactgctag gagttataaa agatgaactt ttagaaataa 7921 aagctaaata tggagatgaa agaagaactg atattgaaca agatatgaat gaaattaata 7981 tagaagatct tattcaagaa gaggatgtag ttataacttt aactaaatct ggatatatta 8041 agagaatttc agcagatgta tactcagctc aaagaagagg tggaagagga atacaagcaa 8101 tgtcaacgaa ggaagatgat tttgtagatc atataacaat aacttcgact cattcagatg 8161 tattattctt tactaataga ggtagggtat acaaattaag agcttacgaa atacctgatg 8221 ctggaaggca agcgaaggga accaatataa ttaacttaat agctattgaa gctgatgaaa 8281 aaatacaaac agtattaaca gtaacagata agcgtagaga tggttattta tttatgggta 8341 ctaagcaagg tattgtaaag aagactcact taaatgaatt tagaaattta agaaaaaatg 8401 gactaatagc tataaatcta agagaaaatg atgaattatt aaaagttaag atcacttatg 8461 gtgatgctaa tgtaatgttc gttactcaaa atggttatgc attaagattt aacgaaaaag 8521 atgttagaca catgggtaga actgcatctg gtgttaaagc tataacatta aaagatgatg 8581 atatagctgt atgtatggat atcgcagttg atgaagaaga attattagtt attagtgaaa 8641 atggatttgg taagagaact cctgtaagtg aatacaagat tcaacatagg ggaggcgtag 8701 gtcttataac ttataagata agtgaaaaga caggaaaatt agttggtgct acagtttgta 8761 aggttgatga tgaattaatg cttataaata ctaacggcgt agctataaga ataaatgtat 8821 cagatatatc aaaaacaagt agagctgcta tgggtgtaac cttaatgaga actaatgaag 8881 aagaaaaaat agttgctata gctaaaatac taaaaagtga tgatgaagat ttagaagaaa 8941 atgaatttga atctaatgaa caaagagaag tagtaaatga agatagcagt ttagatgaac 9001 tagtagagag agcagaagat aatagtgaaa atgatactga tatagaatag ataaagaatt 9061 taactaataa aacaaaaaaa ccatgtaggt atagataaaa tcctacatgg tttttatttt 9121 atatctataa aaaattctaa attaaagcat tttttctata tgcaatgtat agatatagaa 9181 gtaatgtctg ccaggcgtgt cgatataaga aatgttaaga aaatataaga aaaataaaga 9241 ataatatata taattttatt ataacggtat atatagtaat agaggtgtat tatatatatg 9301 gtaagataaa acacgtcgca gagagatgcg atgaacaagt tttaaaaaat taggttttag 9361 aaattaaatt tcaacttaaa aaaacttgtt gacaagaaac aaattaagtg atatacttaa 9421 gaggtcgctt gagggcgaca caataaaaca aaagttgaca tgaagtcagc cgagaaaaat 9481 tggtctttga aaattgaaca gaaatcaaga aacattaaaa taaaccagca attctttatt 9541 tgagtaagtc aagattaaac tttttattga gagtttgatc ctggctcagg acgaacgctg 9601 gcggcgtgcc taacacatgc aagtcgagcg atgaagcttc ttcggaagtg gattagcggc 9661 ggacgggtga gtaacacgtg ggcaacctgc ctcatagaga gggatagcct ttcgaaagga 9721 agattaatac ctcataagat tgtagtttcg catgaaacgg caataaaagg agcaatccgc 9781 tatgagatgg gcccgcgtcg cattagctag ttggtaaggt aatggcttac caaggcgacg 9841 atgcgtagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccagactc 9901 ctacgggagg cagcagtggg gaatattgca caatggggga aaccctgatg cagcaacgcc 9961 gcgtgagtga tgaaggtctt cggattgtaa aactctgtct ttggggacga taatgacggt 10021 acccaaggag gaagccacgg ctaactacgt gccagcagcc gcggtaatac gtaggtggca 10081 agcgttgtcc ggatttactg ggcgtaaagg gagcgtaggt ggatatttaa gtgggatgtg 10141 aaatactcgg gcttaacctg agtgctgcat tccaaactgg atatctagag tgcaggagag 10201 gaaagtggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaagaa caccagtggc 10261 gaaggcgact ttctggactg taactgacac tgaggctcga aagcgtgggg agcgaacagg 10321 attagatacc ctggtagtcc acgccgtaaa cgatgaatac taggtgtagg agctgtcatg 10381 gcttctgtgc cgccgctaac gcattaagta ttccgcctgg ggagtacggt cgcaagatta 10441 aaactcaaag gaattgacgg gggcccgcac aagcagcgga gcatgtggtt taattcgaag 10501 caacgcgaag aaccttacct agacttgaca tctcctgaat tactcttaat cgaggaagtc 10561 gcttcggcga caggaagaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt 10621 gggttaagtc ccgcaacgag cgcaaccctt attgttagtt gctaccattt agttgagcac 10681 tctagcgaga ctgccgtggt taacgcggag gaaggtgggg atgacgtcaa atcatcatgc 10741 cccttatgtc tagggctaca cacgtgctac aatggctggt acaaagagat gcaataccgc 10801 gaggtggagc taaactataa aaccagtctc agttcggatt gtaggctgaa actcgcctac 10861 atgaagctgg agttgctagt aatcgcgaat cagaatgtcg cggtgaatac gttcccgggc 10921 cttgtacaca ccgcccgtca caccatgaga gttggcaata cccaaagttc gtgagctaac 10981 ccgtaaggga ggcagcgacc taaggtaggg tcagcgattg gggtgaagtc gtaacaaggt 11041 agccgtagga gaacctgcgg ctggatcacc tcctttctat ggagaaatct agttaacatg 11101 atgtttaact agtaattaaa atatacttac ttcgaaaaag aaggtttaat ttaatgtaga 11161 ttctgttcaa ttttgaaaga ctaagtcttt caaaatgttc tttgaaaatt gcacatagta 11221 aattgtatat aatacaacaa gccaagaatt attctttgta tttaaataag aactattcat 11281 taattgttaa aattagatta ataggtcaag ctacaaaggg cgcatggtga atgccttggc 11341 atcaggagcc gatgaaggac gcgataagct gcgataagct tcgggtagac gcacatagtc 11401 agagatccga agatttccga atgaggaaac tcacatggga aaccccatgt atcgtaaagt 11461 gaatacatag ctttatgaag gtaaacccag ggaactgaaa catctaagta cctggaggaa 11521 gagaaagaaa aatcgatttt cttagtagcg gcgagcgaaa gggaaagagc ccaaaccaaa 11581 gatttatctt tggggttgcg gacagaacat taacgagaaa ttatggttaa ccgaacacaa 11641 ctggaaagtt ggaccgtagg gggtaatagt cctgtaggtg aaaattataa tgatcaagtt 11701 ctgatccaga gtaccacgag acacgtgaaa ccttgtggga agcagggagg accacctccc 11761 aaggctaaat actacctgat gaccgatagt gaagcagtac cgtgagggaa aggtgaaaag 11821 aaccccggga ggggagtgaa atagaacctg aaaccatgtg cctacaaccg atcaaagcac 11881 cttatgtgtg tgatgatgtg ctttttgtag aacgagccaa cgagttacgg tatgtagcaa 11941 ggttaagtac ttaaggtacg gagccgaagg gaaaccgagt cttaataggg cgactagttg 12001 catgctgtag acccgaaacc gggtgaccta tccatggcca ggttgaagcg agggtaaaac 12061 ctcgtggagg accgaaccac gttgctgttg aaaaagcatg ggatgagctg tggatagcgg 12121 agaaattcca atcgaactcg gatatagctg gttctcctcg aaatagcttt agggctagcg 12181 tcggaaaatg tgagtactgg gggtagagca ctgaatgggc tagggggcat agcgcttacc 12241 gaaccttatc aaactccgaa taccagatac tatcagtccg gcagtcagac tgcgaaagat 12301 aagttccgta gtcaaaaggg aaacagccca gatcgtcagc taaggtccca aagtgtaagt 12361 taagtggaaa aggatgtggg atttctaaga caactaggat gttggcttag aagcagccac 12421 tcattaaaag agtgcgtaat agctcactag tcaagagatc ctgcgccgaa aatgtccggg 12481 gctcaaactt accaccgaag ctacgggttc acacaatagt gtgagcggta gaggagcgtc 12541 gtaatcgggc tgaagtcgta ccgtaaggag cggtggactg attacgagtg agaatgttgg 12601 cattagtagc gagatgtggg tgagaatccc acaggccgaa tacctaaggt ttcctcagga 12661 aggttcgtcc gctgagggtt agtcgggacc taagctgagg ccgaaaggcg taagcgatgg 12721 acaatcggtt gatattccga taccactatt atcgttatta tcgatggtgt gacggagaag 12781 gataggatgt gctagctatt ggatgctagt ctaagcgttt agggagttag aataggcaaa 12841 tccgttctaa caattctgag gcgtgatggg gaaggttcta aggaaccgaa gtatctgatt 12901 ccatgcttcc aagaaaagca tctagaaaga gaaatagtgc ccgtaccgca aaccgacaca 12961 ggtaggtgag gagagaatcc taaggccggc ggaagaattg cagttaagga actaggcaaa 13021 ttgaccccgt aacttcggga gaaggggtgc ctgcgagagc aggccgcaga gaataggcac 13081 aagcaactgt ttaacaaaaa cacaggtctc tgctaaagcg aaagctgatg tataggggct 13141 gacgcctgcc cggtgctgga aggttaaggg gaacacttag cgtaagcgaa ggtgtgaact 13201 taagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc 13261 aggtaagttc tgacccgcac gaatggcgta atgacttgtg cactgtctca actgcaaatc 13321 cggcgaagtt gtagtgcgag tgaagatgct cgctacccgc gattggacgg aaagaccccg 13381 tagagcttta ctgtagctta gcattgaatt tcggtattgt ctgtacagga taggtgggag 13441 actgggaaat cagagcgtca gctttgatgg agtcatcctt gggataccac cctgatagta 13501 ctggaattct aactggatgc catgaatctg gtgacaggac attgttaggt gggcagtttg 13561 actggggcgg tcgcctccta aaaagtaacg gaggcgccca aaggttccct cagaacggtc 13621 ggaaatcgtt cgtagagtgc aaaggcataa gggagcctga ctgcgagacc tacaagtcga 13681 gcagggacga aagtcgggct tagtgatccg gtggtacctc gtgggagggc catcgctcaa 13741 cggataaaag ctacctcggg gataacaggc tgatctcccc caagagttca catcgacggg 13801 gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgaagtagg tcccaagggt 13861 tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc 13921 ggtccctatc cgtcgcgggc gcaggaaatt tgagaagagc tgtccttagt acgagaggac 13981 cgggatggac cgacctatgg tgtaccagtt gtttcgccag aagcatagct gggtagctaa 14041 gtcgggacgg gataaacgct gaaagcatct aagcgtgaag cctccttcaa gatgagattt 14101 cccatagcgt aagctagtaa gaccccttga agactacaag gttgataggt cagaggtgta 14161 agtgtggcaa catatttagc tgactgatac taataggtcg agggcttgac caataaataa 14221 tagttatata caatttatgt gcaattttga aagaacattc tttcaatccg aatctggtga 14281 tgatggcata gaagtaacac tcctttccat tccgaacagg acagttaaga tctatagcgc 14341 cgaaggtact gcatgggaga ctgtgtggga gattaggacg ttgccaggta agggatcttt 14401 agctcagttg gttagagcaa ccggctcata accggtaggt ccggggttcg agtccctgaa 14461 ggtccaccat tttgggggta tagctcagtt gggagagcac ctgccttgca cgcagggggt 14521 caagagttcg aatctcttta tctccaccat ttatttaaaa gctatgaagt taattcatag 14581 ctttttttgt tataattaaa agtaattaac ttttttatta ggagaaacaa aaatgttata 14641 tagagtaaaa caatttatat taggaatact ttcgtatttt gaaagtgata atgttaattt 14701 tataaataaa tatttaaata aaaaagaaaa aaatttattt atgaaattaa gtaagcctga 14761 tagaattcat tctcttaggg tttgcaaaga ggcaattctt ataggaaagg attatgatac 14821 tgtagatgag tttaaaatag ctaaatgtgc attattgcat gatataggca aagtagaaat 14881 ttcattaaat attattgaaa aaggagtcgt agtaattata aataaagtta ctaagaggag 14941 ctttttaaag tataataaat atagtagaat gactaaatat tataatcatc ctgcaattgg 15001 taaagaatta ttagaaaaga taaactttga agataaagaa atattatatt gcatagaaaa 15061 tcatcacaaa aatttagacg aaattgaaga aaatttatat ttaaaaatat taaatatatg 15121 tgatagtaaa aattaagcct aaataaacat aataagttat ggagggtaaa atttgtgaat 15181 tctattgaaa gaagagaaag tatacttaaa agtttaattc aaagtaaaac tccattaaaa 15241 ggcgttgaaa tagcaaataa atatggagta acaagacaaa taatagtaaa agatatagct 15301 atacttagag ctaaaggaaa taatataatg gctactcctg atggatatat tattaataag 15361 gaattaaata aaataaaagc tataatagct gtaaatcata aagaagaaga attagttcat 15421 gaaatgagta tagttattaa gtatggtggt actattgaag atgttatagt agaacatcct 15481 ttgtatggag aaataaaagg gatgttaatg attaaaaatt ataatgaatt aaataaattt 15541 gcagaaaaat ataagaagca acaaggtaga ttattatctg tattaactaa tggtgtacac 15601 attcatacta tatcagcaga tactcaagaa gatataaata gtattataga tgaattaaaa 15661 gaaaatggat ttatagtatc tgattaggag gaaaataata tggattatga tgtactaatt 15721 ttaggtggag gcatagtagg atgtgctgta gcttatcaat tatcaaaata taatattaac 15781 atagcattaa tagaaaaaga ttatgatgtg gctgatgata tatcatttat aaatacagca 15841 gttatttatg atggatcaga aacttctaat gatatgatgg caaaacttga aggtataggt 15901 agaaagttaa ttgaggaaca ttgtaaaaag tttaatgtat catatgaaaa aacaggagca 15961 ttgagaataa catctaataa taatcaagaa tataaattag atattatgta tgatagagct 16021 aagaaaagaa atatagaagg tatccattta atagaagatg ataaagaatt gcttcataaa 16081 attgaaccta atttaaatat agatgttaat aaggcttttt attctgaaaa tgtagctgtc 16141 atttctccat acgatttagc tatatcttat gcagaggttg ctgctgataa tggggtgaat 16201 tttaaattag aggaggaagt aatagatatt caaagtcttg ctcgtggatt taaagtaact 16261 acaaacaaaa ataaatttac atgtaaattt gtagttaata caatacctga tgaaatatct 16321 ttcaataata ctgaagaaga atatgattat aaaaatatga attatataat gtttgaaaat 16381 aaatctgagg ataaattaaa aactattctt atagatgaaa tagatgatac aacttttgtt 16441 ataaataatc ctaaattaca aaatggaaat ttaataggaa taaagagtaa tcatatttta 16501 tcattagaag agggtattga tatgtctgtt agtatatttc ctgaagctaa aaaggatttt 16561 gtgagtaata tgtttgcaga agtatataat aaagatataa tgataataga tgatagtaag 16621 ttaaatagtg ggtatataaa gattacaggc actcactatg gtaaaattac tattgcacca 16681 gccattgcgc ttaagatcaa aaacacttta aaggataatt taaatattat tagaaaaaaa 16741 gattatatag ataaaagaag agaagtatat agatttaacg atttatcaaa taaagaaaga 16801 aatgaagtta tttctgtaga taaaagatat ggaaatataa tttgtgtatg taataatata 16861 tctgaaggtg aaataattga tagtattaga agaccattag gtgctcggac tgttgaaggt 16921 gtaaaaagac gtactggtat tggtagaggt aattgcaatg gatcttattg tgatataaag 16981 ataataaaaa tattggccag agaaatggat aagaatattt tagacatagt agaagactca 17041 aaagattcta atatagtatc tagtagaatt aaggaattca aggaaatata aaatttagga 17101 gatgtattta tgagtagtaa ggatattttt actagtttag ttagagtaaa aggaagtccc 17161 aatcataaag ttgtttctgt aaaaagcaat aatcctatag agaaggaatt atggagggaa 17221 ttttcaaaag ttcttagtag aatttatgtt agtgcaccta tcaatattgg tgatataata 17281 tgtaaaaata taatgaatac tggaatagat attgtgtgta ctaaaaaaat cgataataat 17341 aaataaaaaa gcttataata tattgacaaa tataagcatt ttaatataat atatttaata 17401 caagttaata tgcaaacctt agataaggct agtagtaata ttatgatttt tagagagtta 17461 gtggttggtg taaactaaca ttcatgtatt gtgaatccac ctttgaggga ctgtgaatta 17521 agcagtacgg taatatcgtt aaattttaga gtgaatagca tttatgctat taattagggt 17581 ggcaacgcgg agtttttcg //