LOCUS NC_010723:24 20231 bp DNA linear BCT 25-AUG-2011 DEFINITION Clostridium botulinum E3 str. Alaska E43, complete genome. ACCESSION NC_010723 SOURCE Clostridium botulinum E3 str. Alaska E43, complete genome. COMMENT locus start: 3133302; locus end: 3153532 FEATURES Location/Qualifiers source 1..20231 /organism="Clostridium botulinum E3 str. Alaska E43, complete genome." gene complement(0..1859) /gene="" /locus_tag="" /db_xref="3133302..3135161" CDS complement(0..1859) /gene="" /locus_tag="" /product="choline/ethanolamine kinase family protein" /translation="MLEDRLEILKILNNNFNINQRELSKRTNISLGKVNSILKEFTLD GTIERVINNRGILYRVTEKGMKILEENLASAKNTRLKIHEENNKSVKEAVILAAGRAR DFGKPVGMLEIEDFKLIDRTLNILKENGINKITIITGYESACYEEYFKNNKNINLVKS DKYKWTGTMYSLSLAREYVKDDFLLIENDLIFEKRTIKELIESNSRDCILLTNESGSG DEAFVEIRNNHLFKMSKDIHQFNRIDGEMIGITKVSHKLYQMMLEEFKDNINPYLNYE YVLLDVARDYNIGFVKIDDLAWGDADTKKEYENILNHLYPTIRRRELNYEINTVKTIV REAMKVSEDEISEVVAAGGMTNKNYRICVKGKRYILRVAGIGTECMISRKNEMFNSSI ASEREYNVETPYFNVETGIKISTFIENAETLTPRSVKKEENLKQVTRILRDLHEDNEF PMKNEFNVFRELEKYEDILKTADGEFFDDYDEVRERFMKLEQVLKECDRVFVPSHNDL VSENLVKDTEGRIYLIDWEYSGINDDMWDLAALSLENNFSEDDTELMFRLYFNGEVDE NSRKRLLIHQISQDLLWAVWTLIKENEGDDFGTYGIDRYNRGKENLNKLEAEF" gene complement(2567..3112) /gene="" /locus_tag="" /db_xref="3135869..3136414" CDS complement(2567..3112) /gene="" /locus_tag="" /product="hypothetical protein" /translation="MIEVRYKNTEEQWVNFSIYKMNSQSNKDRLATLRRNLFLGIGLF IGVLYCVLGIQEYSKGESPIFSIVLGVIFAGAGIFGYFKLPTYWIKRAKKNFKDLFKK NEKSLGRTIEVFLEKDGLIINRNKEKTKLLLNNITDVTEINNCLYVISNDNPGIVIPN DAFESTDDKINFKNTIMNNIK" gene complement(3480..4103) /gene="" /locus_tag="" /db_xref="3136782..3137405" CDS complement(3480..4103) /gene="" /locus_tag="" /product="hypothetical protein" /translation="MQVNNNGINNNKLYTNYNQEINNINNKSKNNDINIKDNSKDKLD LSKDMRTAASRSRAIMGPQLDKGTASNTTIYVDKSTFMQIANYSTNNSECQWDELGVD GEKRWVVINGQRFECPLSKEEKEAFERASKTMLDYLIEADKEKEEKKEKKENIDFKFN GDGSISIDDESLLSSNPKLSGLLKNDKVMKMLKDIAKTNGGSLSMHI" gene complement(4399..7383) /gene="" /locus_tag="" /db_xref="3137701..3140685" CDS complement(4399..7383) /gene="" /locus_tag="" /product="putative collagenolytic protease" /translation="MGEKINKKRIISLFVALTCIFSLLPTSFGMNKVQAATTNTSGRA TLDITGPQYALTVSGTKLEGNTIDTGVKVDSTKTTYNTQGRFKDFQIDLPKYKQEVTD KKTDKDELQGKITEVETITKIDRTIDIASINKIKKDLLSEIDVTLTQTQNKSTSNEVT NGITIENLPLGTNEVMYEISEKTTTDIKTTVYKINADGSISKDSEDNSQTTDLITSKS TQIIIEHAENYAQGKIKKIDFKSYVGSKDTYLQDLKDGNKNKNKVPFLYNKELLTTDT ESFFTYEHIVNDQIQNLDYIINFDESFKLDGAKVWMDGTSSTASTSGNTLEGALEKTD SKIMVIKMPNNGTLGKNYAIKLKFIDENSSNDYTLRKVDIQSKYNNEEILLQEKNYID KEFKDVSVPGEPKRYEGKIYLDKNAGKVNITPNLGKSSGYTVKLSNHYLDEYGKVKIK QQDFDKFVSFSDSTSDKPNANVIYMDIYEGSESSEQLGALLATYELQVVFNGKQQPYK FNFGDNVTLKEANGGEKEINFNSSIHKYDLYVKDPSQKVRITYEDGLVETVARVKVNG IEESIQIKGSLEFDLRNSGGKQLTITIYKGGTLASDKYVFNIQGDSDEGGGEIPSAQN AYLSYLNFNTGDLKKEDGSAGFSRETLNYDLVVKPDTKVVDVTAIVDEPKASITEAKV IETGENYNLTSGTKSQIPLQESGTTTLQIVVTAENGKDKKMYTVVIKHDGRSNNADLK DIELNTGDYEFNSKHDTFKARVDQDVKSVKVKPIPVDENYSSITVDGEKFTGSPISIS LKGQWKTEVDIVVVAEDKSTKKTYELTIYRTNDPLDDIKDPDDDDDDDDDDDKNDAYY DESNKIWIDMSKYEEWGKVNGKYIYFDKRGRQVKDAWVKVDGSWFYVNKSGYRDTGWI KQSDGRWYYLDNNGRMITGWFYDKERGNQYYFNPNGAMQVGWLYLGGNWYYFESGGRM LQNEKAYIDDAWYSFSNFGIMY" gene complement(7832..9889) /gene="" /locus_tag="" /db_xref="3141134..3143191" CDS complement(7832..9889) /gene="" /locus_tag="" /product="surface protein PspC" /translation="LNINKKVISLFLALTIVFSIVPTINVQASTTDNSSEVVYEGEKE TLLASTRANFADIKIVSDTEVTAKQAKKWAESKGATSTFANLAELYWKYAEKCGDVNP GIAYVQAAKETGYGKFGGVLDESYNNPCGMKTSQGGGDYDPNAHQRFNSWDEGVQAHL DHLALYAGAKGYPKEGTYDPRHFVSIKGKASTVNSLGGRWAPSPTYGKDVNTLYNNLL AYSGIDSEYEENTKPDGPGSDEEEDYTPEFPEAGTEPSIPEITKPVEDSNDNSTNISS SIGWKSENGNWYYYKSDGTKATGWIKPDGNWYYLYSDGSMATGWLQTKGNWYYLQSWG GMQKGWLNDGGKWYYLQGDGIMVTGFSSINGKTYFLDVSGAMRVGWFEISGNWHYFNT DGSMLNGWIKPYEHWYYLYSNGAMAKGWIKLNGDKWYYLNNSGDMATGWTTVGSDTYY LNPSSGEMLKDTVIDGWKIGPDGKRLKRVDGGSSSSKLIVIDPGHNYGGDDGAYATHN RVTYSERDLNMQLSMKLKVKLESNGYQVVMTRNETDRETLSVTQSLTKRVNLANELNA DFFVSVHHNSASAASASGVETYYSTKAQDSNFGGTYSDSKISTSRRMATNITNSIVNK TGATNRGGKDGNLFVCRNTKMPSVLVESGFISNPTEAANCADSNYQHKIADGIAEAVS NAF" gene complement(10190..12403) /gene="" /locus_tag="" /db_xref="3143492..3145705" CDS complement(10190..12403) /gene="" /locus_tag="" /product="surface protein PspC" /translation="LKIKSKIVSFLFILVMTLGFMPSIMVHASVKDINIISDTEATAN DAKKWAKTKGATEEFLSLADLYWKYSEDCGDVNPAIAYVQSAKETGFGRFGGVLDSSF RNPCGMKTSQGGSDGDKYAHKKFNTWDEGVQAHMDHLALYAGAKGYPKSDTYDPRHFI SIKGKSPTVNSLGGKWAPSITYGEEVNKYYIELCSYSNIAVDKDEQKDDDDDLNDNDD NNSSHFSPPGPVENKPNSLVTDSVIIFDNSFVEPTLNRETNVSSEIGWKEEDGKWYYY YSDESKAAGWINPNGNWYYLNSTGEMSVGWTNLENSWYYFDDEGIMVTGWKCINNNWY YLKTSGIMATGFLQNANDLYYLDKSGAMLSKEGWNLIDGKWYYVENGGKLKLGWLLDN GNKYYLQGDGSMVTGTKLIDNKTYVFDQNGINKTGWININNYWYYFNENSEMSTGWLN LNGTHYYLYDTGAMATGWLSIDEDWYHFSNDGSRSLGWVQSGGYYYYLDLQSGKLLKN TKVDGYEIDSNGRRKSDKNDITPGNTSKNVIVVDAGHNFGGDDGAYATNNGVTYSERD LNMEVAVKLKSELENRGYTVAMTRNESDRETLSAMQSLDKRVKLANDLNATLFVSVHH NSADAVSANGVEVFYSTNAQDDRMGGKSPDQSRIEKSKAMATSVVNNICSKTGAINRG PKNGNLNVCRNTNMPAILIECGFITNANEAERCADSNNQTIVAKAIAEAIQNQLN" gene 12749..13018 /gene="" /locus_tag="" /db_xref="3146051..3146320" CDS 12749..13018 /gene="" /locus_tag="" /product="putative anaerobic ribonucleoside-triphosphate reductase" /translation="MNIIKKNGRIEEFKISKVRQSILSASEEVTEPLSDAELKLIEKE VNNILSKLNRTETSSYEVFGICLNALNELGFKAVCKSYLQGSISF" gene 13273..14100 /gene="" /locus_tag="" /db_xref="3146575..3147402" CDS 13273..14100 /gene="" /locus_tag="" /product="phosphatase YidA" /translation="MKYKLICIDMDGTLLNGHSSISERNKDALKKAVDKGVQVAISTG RIFASADYFAELIGIKTDLISCNGAYIKNRSTNEVIYNNTLTNEQVLKIHNSIKGHNF RIMYYTHDTAIVDSPFPENHTYNLTNKLVSDEKKVKFFVTSDINKILEIYSNKIIKVI CIDDSDDKDTLFKVKDNLLKFSDIETVSSGDNNFEIMQKDVSKGHAAKILSEKLGIKP EEVICIGDNENDLSMIKFAGLGVAMGNGSDVVKKIANYITDTNVNDGVAKVIEKFIL" gene 14403..15233 /gene="" /locus_tag="" /db_xref="3147705..3148535" CDS 14403..15233 /gene="" /locus_tag="" /product="pyridoxine kinase" /translation="MNKNKQKRIAVINDLSGFGRCSLTVALPIISAMKIQCCPLPTAI LSANTAFPNFFFDDYTNNMKNFTNHWQEINLSFDGICTGFLGSKNQIDIVIDFIKKFK NNNTHVLVDPIMGDNGKIYSTYTDEMCNEMKSLIKYADIITPNLTEACKLIDMPYPNR ILDEKELEFIAKSLCENGPKKIVITGLQNHDYVQNYIYEIGKPPKIISVKKIGKDRCG TGDVFSSILAADMVNNLDIIDSVKKASDFISKCLEYTSELDISVHEGICFEEYLCELK" gene complement(15368..15679) /gene="trx" /locus_tag="trx" /db_xref="3148670..3148981" CDS complement(15368..15679) /gene="trx" /locus_tag="trx" /product="thioredoxin" /translation="MSKILKVNEFDDAIKEGIVIVDFFADWCGPCKMLAPIFEELEEE MKDKVKFFKVNVDESGELASKFSVFSIPTMIIFKDGKDVSTEVGFLPKEKIKMNLEKY I" gene complement(15790..16656) /gene="" /locus_tag="" /db_xref="3149092..3149958" CDS complement(15790..16656) /gene="" /locus_tag="" /product="pyridine nucleotide-disulphide oxidoreductase" /translation="MGERYDIAIIGSGPAGLSAALNARIRKKNFIIFGSKDISNKLVK AHTINNYLGFYGKSGLEIRDEFLNHLQSMDINITEEKVNSIYAMGDYFSLMVNEKMYE ATSIILATGVNFGKPFNGEEEFLGKGVGYCATCDAPLYKDKVVTIIAYNKKDEEEANF IASIASKVYYVPMYNEEVEVNSDIEIIKDKPVEIKGDMKVNSLILSNKELKTDGIFIL RDSISPGQLVPGLELDQNHVKVDRLMKTNIKGCFAAGDIVGVPYQYIKSAGEGNIAAL SAVSYVDEKNKR" gene 17165..17962 /gene="" /locus_tag="" /db_xref="3150467..3151264" CDS 17165..17962 /gene="" /locus_tag="" /product="histidinol phosphate phosphatase HisJ family" /translation="MIYYDYHMHCNYSADSQAPMEDMIEKSIELGLKEICFTDHVDYD VLGNPNVKVDYDKYFEDIDFYNNKYKDKISIKKGIEMGLQTQVLERCSKEIKETNFDF VIGSIHTIDRNELFTGDFHKGKTQHEAYEGYYKSLLEIVNSYDDYSVLGHVDLIKRYG NYDTILKDEIFSDYLEAILKKVIYSGKGIEINTSSFRYNLPDLTPSKNILKMYKDLGG EILTIGSDSHNPTQIATKFDYIHDALGSMGYKYICKFNNMKPEFIKL" gene complement(18176..20230) /gene="" /locus_tag="" /db_xref="3151478..3153532" CDS complement(18176..20230) /gene="" /locus_tag="" /product="membrane assosiated methyl-accepting chemotaxis protein with hamp domain" /translation="MRFKKISTRMLTILLFVTILSMVLLSGISYKSSKNIIDKQVEEN MQAELSSTVNAINLKMQEISSMSSQLALNVGSTYKTTYLSQYEEMIGKVIFKSDLVLG AGIWFEPYVYDASKEYVGPYIHKNDDKALVTYDYSNAEYNYFNYDWYKNAKNNNKETV FSDLYYDETLNTIMSTCATPIYDSNDNFIGVISVDMEIPSIQDLINNFKIGENGKALL INNDGKYITNIDDKKIMETKINEDENKSLSEFGNVLLNNTNGKSEFSIDDTKYESYYT TVEPFGWKLFIQIPMSELNKPINELMSKLIVVGILAIILTTLAIIWQVRYLTKTIKKV NDLASNLSNGDFSINELEVISEDELGQMSNSLNKMLSENKNMIQEISDDANKVNSITE NLNKSTESLVENYKVIKDSVKNISESMLNSSATTEEVNASVEEVNSSISLLSQETIKS HDMAKDIKVRAYNIEKMSNDSYNKATSLSGVHETNLKQSIENAKIVENIGAMAKAISD IAEQVNLLSLNASIEAARAGEQGKGFAVVASEIGNLATQTSDTVKEIIETISKVQEAF NKLTNDSMQMLLFIKDTVTPDYKEFVGIANQYDKDANAIEDISIKISEMTNNIEYITH EVGDAIQNIAEASQDTASNSGEIVSSIELVSQLVDEISTIVENETEVSDNLNSMVKKF TL" ORIGIN 1 ttaaaattca gcttctagtt tatttaaatt ttcttttcct ctattgtatc tatctattcc 61 ataggtacca aaatcatccc cttcattctc tttaatcaat gtccatacag cccataataa 121 atcttggctt atttgatgaa ttaataatct ttttcttgag ttctcatcta cttcaccatt 181 gaaataaagt ctaaacatta attctgtatc atcttccgaa aaattatttt ctaaagaaag 241 tgctgctaaa tcccacatgt catcatttat accgctgtat tcccagtcta ttaaataaat 301 tctaccttct gtatccttca ctaaattttc tgatactaaa tcattatgag atggaacaaa 361 aactctatca cactctttta gaacttgttc taatttcata aatctttctc ttacttcatc 421 ataatcatca aagaattctc catcagcagt ctttaaaata tcttcatatt tttcaagttc 481 tctaaacaca ttaaattcat ttttcattgg aaattcatta tcctcatgta aatcccttaa 541 tattctagta acttgtttta gattttcttc ctttttaaca cttcttggag ttaaagtttc 601 tgcattctct ataaaagtag atatttttat tccagtttca acattgaaat atggagtttc 661 aacattgtat tctctttctg aagcaattga agagttaaac atttcatttt ttctacttat 721 catacattca gttccaatac ctgcaactct taatatatac ctttttccct taacacaaat 781 tctataattt ttattagtca tacctccagc agctacaact tcacttatct catcttcaga 841 tactttcata gcttctctaa ctatagtttt tacagtgtta atttcataat ttagctcacg 901 tcttcttata gttggataaa gatgatttaa aatattttca tattcttttt tagtatctgc 961 atctccccaa gcaagatcat ctatttttac aaatccaata ttgtaatctc tagcaacatc 1021 taataataca tattcataat ttaaatatgg atttatatta tctttaaatt cctcaagcat 1081 catttgatat aatttatgag ataccttagt tataccaatc atttcaccat caattctatt 1141 aaattgatgt atatccttag acatcttgaa tagatgatta tttcttattt caacaaaagc 1201 ttcatcccct gatccacttt cattagtaag aagaatgcaa tccctactat tactttctat 1261 aagttcttta attgttcttt tttcaaaaat caaatcattt tctattaata aaaaatcatc 1321 tttaacatat tctctagcta aagataatga atacatagtt ccagtccatt tgtatttatc 1381 acttttaact aaatttatat tcttattatt cttaaagtac tcttcataac aagcactttc 1441 ataacctgtt attatagtaa ttttattaat tccattttct ttaagtatat ttaaagttct 1501 atctattaat ttaaaatctt ctatttcaag cattcctaca ggttttccaa aatccctagc 1561 tcttcccgct gcaagaataa cagcttcttt aactgattta ttattctctt catgaatttt 1621 caatctagtg tttttcgctg atgcaagatt ttcttctaat attttcatgc ctttttcagt 1681 taccctatat aaaatccctc tattgtttat aactctttct atagttccat caagtgtaaa 1741 ctcctttaaa attgaattca cttttcctaa tgaaatattt gttctctttg acaactctct 1801 ttgattaata ttaaaattat tgtttaatat ttttaatatt tctaatctat cttctaacat 1861 ataacctctc cccctttaat ttgtaaacca catatttaat tctttgatct tctaatataa 1921 atctcatgga gtactgtcta ctaaaaaaac tctgataaaa gtaaattcca acaccatatc 1981 tgattatact aaaacataaa aatctagtta aaaatatata aataattata tatatctaaa 2041 attattttat ccaaaattct aatttttagg cttataacct ataacacaat tgaacataca 2101 gaatttagaa ttctgccaaa acatatactt tataaaaaat atatctttaa atccaattaa 2161 aaatttttta tcattaaatt tttcctttta aaatacaaaa taaccgcaat ctctgttcat 2221 aatatgaact ttaataaatt atattataat tcatataaaa agtaaataca tttatcaaat 2281 ttttaccata taattttaat agaaagttta attaataaat ataataataa tttttgatac 2341 tcagcattga gtcaaaaaaa atgctagtct aataaaaaat aagctatatt ttaagttaaa 2401 ttgcaactta aaatatagct ttaattatta tattaatgtt taataccaat agaacaattt 2461 atctagacga ctttaccaat acatatatcc aaaacgtagt tcagaataac ggtgtggcaa 2521 tttacatatt aaattactta attcaaattt atctataaat atattaacta ttttatatta 2581 ttcattatag tatttttaaa atttatctta tcatctgtac tttcaaatgc atcattagga 2641 ataactattc ccggattatc atttgaaatt acatataaac aattatttat ctctgtaaca 2701 tctgtaatat tatttaataa aagttttgtc ttttccttat ttctattaat tatcaacccg 2761 tctttttcca agaatacttc tatagttctc cccaaagatt tttcattttt cttaaataaa 2821 tccttgaaat ttttctttgc tcttttaatc cagtatgtag gcaatttaaa atatccaaat 2881 atacctgcac cagcaaaaat tacacctaat acaatagaaa atatcggact ttctccttta 2941 ctgtattctt gaattcctaa aacacaatac aacacaccta taaaaagacc tattcctaaa 3001 aataaatttc ttcttaacgt agccaatcta tctttattac tttggctatt cattttataa 3061 atagaaaaat taacccattg ttcttcagta tttttatacc ttacctcaat cattctcttc 3121 ccctcacact ctcgttaata tataaattaa tgtttatttt aatatatttt taaactttaa 3181 attagatcga aatcaagatt tacctatatt ataactttaa ctttataata taataattta 3241 acacaataat cactttgcaa tatatatttt atattaaaat atactaatta atttatatta 3301 taattatatt acaatttgta aacatatcca ataacattta tatatacttt tgcatagtat 3361 aaattatagt ttttcctctc attttatata aatatatata gaaatagaat acatacttaa 3421 atcttatact tattacaaaa cttacttact atgtattcta tttctaatta aagtatataa 3481 ttatatatgc atagataaac tgccaccatt agtttttgca atatctttaa gcattttcat 3541 cactttatca ttctttaata atccacttaa ctttggatta ctacttaata aagattcatc 3601 atctatagat atgcttccat caccattaaa tttaaaatct atgttttctt ttttctcttt 3661 cttttcttct ttttccttgt cagcctctat tagataatca agcatagttt ttgaggctct 3721 ttcaaaagct tccttttctt ctttacttaa tggacattca aatctttgtc catttattac 3781 aacccatctt ttttctccat ctacaccaag ctcatcccat tggcattctg aattatttgt 3841 tgaataattt gctatttgca taaatgttga cttatctaca taaatagtag tattactagc 3901 tgtaccctta tctaattgag gtcccattat ggctctactt ctactagccg ccgttctcat 3961 atctttagat aagtctaatt tatctttaga attatcttta atatttatat cattgttttt 4021 tgatttatta ttaatattat ttatttcctg attataatta gtatatagtt tgttattatt 4081 tattccatta ttattaactt gcataattat ccccctcttc acttcttact ttattatcga 4141 tataattcat ttcttcttta ttataattaa atattattct ttcttaatta gtaactaact 4201 cttataagct ataattctca taaatttatt atgtaataag actctacttt aaatatgaat 4261 ttatataaac aatagaatca attcatttac tctttcaaaa ttacttattt ctatatcatc 4321 acactaccaa aatataatgt ataaaaaaat gagatgattc ctttatatat aatcatctca 4381 tttttatctt atataatttt taatacatta ttccaaaatt agaaaatgaa taccaagcat 4441 catctatata tgctttttca ttttgaagca ttctaccacc tgattcaaaa tagtaccagt 4501 tcccacctaa gtataaccag cctacttgca ttgctccatt tggattaaag taatattgat 4561 ttcctctttc tttatcataa aaccaaccag ttatcattct tccattatta tcaagatagt 4621 accatctgcc atcactttgt tttatccagc cagtatctct atatcctgat ttgttaacat 4681 aaaaccagct accatcaact tttacccaag catctttaac ttgtctacct cttttgtcaa 4741 agtaaatata ttttccattt acttttcccc attcttcata cttgctcata tcaatccaaa 4801 ttttattact ttcatcataa tatgcatcat ttttgtcatc atcatcgtcg tcgtcgtcat 4861 catcatctgg atctttaatg tcatctaaag gatcattagt tctatatata gttaattcat 4921 aggtcttctt tgtactttta tcttcagcta ctacaactat atcaacttct gttttccatt 4981 gacctttaag acttattgat attggacttc ctgtaaattt ttctccatct acagtaatac 5041 ttgaataatt ttcatctaca ggtattggtt taactttaac agattttaca tcttgatcaa 5101 cacgtgcctt aaatgtatca tgtttagaat taaattcata atcacctgta ttaagttcta 5161 tatcttttaa gtcagcatta ttacttcttc catcatgttt aataactaca gtatacatct 5221 tcttatcttt accattttca gctgtaacaa ctatttgtaa tgttgttgtt ccactttctt 5281 gtaatggaat ttgactttta gtacctgatg ttaaattata attttcacct gtttcaataa 5341 cttttgcttc tgttatactt gcctttggtt catcaactat tgctgttaca tcaacaactt 5401 tagtgtctgg tttaactact aaatcataat ttaaagtttc tcttgaaaat cctgcacttc 5461 catcttcctt ctttaaatct ccagtgttaa aattcaagta acttaaatat gcattttggg 5521 cacttggtat ttctccacca ccctcatctg aatctccttg aatattaaat acatacttat 5581 ctgatgctaa agttccacct ttataaatag taatagttaa ttgttttcct cctgaatttc 5641 tcaaatcaaa ctcaagactt cccttgattt gaatagattc ttcaattcca ttaaccttaa 5701 ctcttgctac tgtttctact aaaccatcct cataagtaat tcttactttt tgtgatgggt 5761 cctttacata taaatcatat ttatgaatag atgaattaaa gtttatttcc ttctctccac 5821 catttgcttc ttttaatgta acattgtcac caaaattaaa tttatatggc tgttgttttc 5881 cattaaatac aacttgtaat tcatatgttg ctaataatgc gcctaattgt tctgatgact 5941 cagaaccttc ataaatatcc atataaataa catttgcatt tggtttatca ctagtgctat 6001 cactaaaaga tacaaattta tcaaaatctt gttgctttat ttttactttt ccatattcat 6061 caagataatg attactaagt ttcactgtat acccactgga cttccctaaa tttggtgtaa 6121 tatttacttt tccagcattt ttatctaagt aaattttacc ttcatatctt tttggttctc 6181 ctggtacact aacatcttta aattctttat ctatataatt tttttcttgt agaagtattt 6241 cttcattatt atatttactt tgaatatcta cttttcttaa tgtatagtca ttactagagt 6301 tttcatctat gaactttaat tttatagcat agttctttcc taaagttccg ttattaggca 6361 tctttataac cataatctta gaatctgttt tttccaatgc accttctaaa gtgtttccac 6421 ttgtgcttgc agttgatgat gttccatcca tccatacctt ggcaccatct aatttaaatg 6481 attcatcaaa atttattata taatctaaat tttgaatttg gtcatttact atatgttcat 6541 aagtaaagaa actttcagta tcagttgtta aaagttcttt gttatataaa aatggaacct 6601 tattcttatt tttatttcca tcttttaaat cttgtaaata agtatccttt gaacctacat 6661 aacttttaaa gtcaattttt ttaatcttac cttgagcata attttcagca tgctcaataa 6721 ttatttgtgt tgatttacta gttattaagt ctgttgtttg agaattatct tctgaatctt 6781 ttgaaatgct tccatcagca ttaattttat atacagtagt ttttatatct gtagtagttt 6841 tttcacttat ttcatacata acttcattag ttcctagtgg aagattttct attgtaattc 6901 cattagtaac ctcattagaa gttgatttgt tttgagtttg agttaaagtt acatcaattt 6961 cacttaaaag atcttttttt attttattaa ttgaagcaat atctattgtt ctatctatct 7021 tagtaattgt ttcaacttct gttattttac cttgtaattc atccttatca gttttcttat 7081 cagtaacttc ttgcttatac tttggtaaat cgatttgaaa atctttaaat ctaccttggg 7141 tattataagt agttttcgta ctatcaactt ttacaccagt atcaatcgta ttaccttcaa 7201 gcttagtacc tgaaactgtt aatgcatatt gaggaccagt aatatctaaa gttgctcttc 7261 cactagtatt cgtagtagca gcttgtactt tattcatgcc aaatgatgtt ggcaataaag 7321 aaaaaataca tgttaatgct acaaatagtg atataattct ctttttatta attttctccc 7381 ccatgttcat cctcctaata ttttttccca aatttattat atatacttat atattatcat 7441 attttagcaa taaaagagta tatcctaatt agacttattt ctaattattt ctcaggaaat 7501 attcaaaaaa atacacttat tttaaaaatg ttaattatat tatattttcg tccaaatata 7561 atatagcttt atatagaaaa ataatgaaat tatataattt cttaatataa taataaaatt 7621 tcctaggaga ttttaaatcc agctatttct agctattcac cctttataaa cctagtacag 7681 caatacttgt acgtcattat tttttattct ttagatatac tcattagacc tttaaagtac 7741 agtattctag aaattacttt caaaatttta tatttttata atttcataaa gaaaaaagga 7801 ggcatatgcc tcctttaatt ttattatcta ttttaaaatg catttgatac agcttctgca 7861 ataccatctg ctattttatg ttgataattt gaatcagcac aatttgctgc ttctgttgga 7921 ttacttataa atcctgattc tactagtact gatggcattt ttgtatttct acatacaaat 7981 aaattaccat ccttgccacc tctattcgta gcacctgttt tatttacaat gctatttgta 8041 atattagtag ccattcttct acttgttgaa atcttagaat cgctataagt tccaccaaaa 8101 tttgaatctt gagcttttgt actataataa gtttctacac cactagctga tgctgctgat 8161 gctgaattat gatgtacact tacaaagaaa tcagcattaa gttcatttgc tagattaact 8221 ctcttagtaa gactttgagt aacacttaat gtttctctat cagtttcatt tctagtcata 8281 acaacttgat atccattaga ttcaagcttt acttttaact tcattgaaag ctgcatattt 8341 aaatctcttt cagaataagt tactctatta tgagttgcat aagcaccatc atctccacca 8401 tagttatgac ctggatctat tactatcagc ttagaagaag atgaaccccc atcaactctc 8461 tttaatcttt ttccatcagg acctattttc caaccatcaa taacagtatc tttaagcatt 8521 tctcctgatg aaggatttaa ataataagta tcactaccta ctgtagtcca acctgttgcc 8581 atgtctccac tattgtttag ataataccat ttatctccat taagttttat ccaacccttt 8641 gccatagcac cattagaata taggtaatac caatgttcat atggtttaat ccatccattt 8701 agcatacttc catcagtatt gaaataatgc caatttccac ttatttcaaa ccaacctact 8761 ctcatagctc cacttacatc tagaaaataa gttttaccat ttattgaact aaatcctgta 8821 accataattc catcaccttg aagatagtac cacttaccac catcatttaa ccaacctttt 8881 tgcattccgc cccaagattg aaggtaatac cagtttccct tagtttgtaa ccaaccagtt 8941 gccatagaac catcactata taagtaatac caatttccat ctggctttat ccaacctgtt 9001 gctttagttc catccgactt ataataatac caatttccat tttcactttt ccaaccaatt 9061 gatgatgaaa tattagtact attgtcattt gaatcttcca caggttttgt tatttctggt 9121 atgctaggtt ctgtccctgc ttctgggaat tctggagtat aatcttcttc ctcatcagag 9181 cctggaccat ccggttttgt gttttcttca tattcactgt ctattcctga gtaagctaat 9241 aaattattgt ataatgtatt aacatcttta ccatatgtag gactcggagc ccaccttccg 9301 cctaatgaat taacagtgct tgctttccct tttatactta caaaatgtct tggatcatat 9361 gtaccttctt ttggataacc cttagcacct gcatataatg caagatgatc taaatgagct 9421 tgtactcctt catcccaaga gttaaatctt tgatgtgcat ttggatcata atctcctcca 9481 ccttgtgacg ttttcattcc acaaggattg ttgtagcttt catccaatac cccaccaaat 9541 ttaccatatc cagtttcttt ggctgcttgt acatatgcta ttcctggatt aacatctcca 9601 catttttctg catatttcca ataaagttct gctaaatttg caaatgtgct tgtagcacct 9661 tttgattctg cccacttttt agcttgttta gctgtaactt ctgtatctga tactatctta 9721 atatcagcaa agtttgctct agtactagct aataaagttt ctttttctcc ttcataaact 9781 acttctgaag aattatctgt tgtactagct tgtacattaa ttgtcggaac aattgaaaat 9841 acaatagtta aagcaagaaa taatgaaata acctttttat taatattcaa aatctttctt 9901 tccctccatt cccatctaaa atatatgctt taaaatattg tctacaaaat tctccaaaaa 9961 atttgcatta atattctaaa atcacactaa ttctaacata tgcaaaaaaa aaagtaaatt 10021 tttataatta cctttctaat tatgtctaaa gcctttattc tacttaatta tactataatt 10081 agctagtttt ttatggtttc attttccata gaaaaaataa aaaaatcttc cacttaaaca 10141 ttaatgttaa gtaaaagatt cttttattta aattctctta ttttgtatag ttaatttaat 10201 tgattttgaa ttgcctctgc tattgctttt gctacaattg tttgattatt tgaatcagca 10261 catctttctg cttcattagc attagttata aatccacatt caataagtat tgcaggcata 10321 ttagtatttc tacaaacatt taaatttcca tttttaggac ctctatttat agcgcctgtt 10381 ttactacaga tattatttac tacacttgta gccattgctt tgctcttttc aattcttgat 10441 tgatctggtg attttccacc cattctatca tcttgagcat tagtactata aaatacttca 10501 actccatttg cagatactgc atctgctgaa ttatgatgta cacttacaaa taaagtagca 10561 ttaagatcat tagccaattt aactctttta tcaagactct gcatagctga taatgtttct 10621 ctatctgatt catttctagt catagcaaca gtataacctc tattttcaag ttctgattta 10681 agttttactg caacttccat atttaaatct ctttctgaat aagtaactcc attgtttgta 10741 gcatatgcgc catcatctcc accaaaatta tgacctgcat caactacaat tacattttta 10801 gaagtatttc ctggagttat atcattttta tcagattttc gtcttccatt actatctatt 10861 tcataaccat caacttttgt atttttaagt agctttccac tttgtaagtc taagtaatag 10921 taatatccac cactttgtac ccatcctaaa cttctacttc catcattact aaagtggtac 10981 caatcctcat caatacttaa ccatcctgtt gccatagccc cagtatcata taagtaataa 11041 tgagttccat ttaaattaag ccaccctgta ctcatttctg aattttcatt aaagtaatac 11101 caataattat ttatatttat ccaacctgtt ttattgattc cattttgatc aaatacataa 11161 gttttattat ctattaactt tgttccagtg accatacttc catcgccttg aagataatac 11221 ttatttccat tatctaaaag ccaacctaat tttaatttcc caccattttc aacatagtac 11281 cattttccat ctattaaatt ccaaccttct tttgaaagca tggcacctga cttatctaag 11341 taatataaat catttgcatt ttgtaaaaat ccagttgcca ttatgccact tgtcttcagg 11401 taataccaat tattatttat acatttccaa ccggttacca taataccctc atcatcgaaa 11461 taataccaag aattttctaa atttgtccat cctacagaca tctctccagt agaatttaaa 11521 tagtaccaat tcccattagg attaatccat ccggcagctt tgctttcatc tgaataataa 11581 tagtaccact ttccatcttc ttctttccac cctatctctg aactaacatt tgtttctcta 11641 ttaagcgttg gctcaacaaa agaattatca aatataatta cagaatcagt tactaaagaa 11701 ttgggtttat tttcaactgg tcctggtggt gaaaaatgag atgaattatt atcatcattg 11761 tcatttaaat catcatcatc atctttttgt tcatctttat caaccgcaat atttgaataa 11821 ctacataatt ctatataata tttatttact tcttctccat aagtaatact tggggcccat 11881 tttccaccta atgaattaac agttggtgat ttacccttta tacttataaa atgtcttggg 11941 tcatatgtat cacttttagg ataaccttta gcccctgcat aaagtgctaa atgatccatg 12001 tgagcttgta caccctcatc ccaagtatta aactttttat gagcatattt atctccatct 12061 gatccacctt gtgaagtttt cattccacat ggattacgaa aactcgaatc caatacccca 12121 ccaaatcttc caaatccagt ttcttttgct gattgtacat atgcaatagc tggattaaca 12181 tctccacagt cttctgaata cttccaataa aggtctgcta aacttaaaaa ttcttcagtt 12241 gcgcctttag tctttgccca tttttttgca tcatttgcag tagcctctgt atctgatatt 12301 atatttatat cttttacaga tgcatgtacc ataattgatg gcataaaacc taatgtcatt 12361 acaagaataa ataaaaatga cactatttta cttttaatct tcaaaatagt attccccctt 12421 cctagttgtc acatttttta acataattat ccatttttct tataaatata atctaatttt 12481 atcatatgaa gttgttaatg taaattatag ttacaatgga taattaatct atgattactt 12541 tttttcttaa ctaaaatata cgtctaatat aataacttca cctttttttt aataatgatc 12601 tatttttaat aaaactctta cttattgcaa attctaatat aaatccctat aattccaaac 12661 aatatattat attagtctat taaacattta ctttatgtac cgtttaaccg ataatataag 12721 taaacattta taagtcagga gatgattata tgaacataat taaaaagaat ggacgaattg 12781 aagaatttaa aatctcaaag gttagacaat caatacttag tgcatcagaa gaagttacag 12841 aaccattatc agatgctgaa cttaaattga tagaaaaaga agttaataat attttaagta 12901 aattaaacag aacagaaact tcttcttacg aagtatttgg tatatgctta aacgcattaa 12961 atgaattagg ctttaaagca gtatgtaaat cttatcttca aggctcaata agcttttaaa 13021 atgcgtacat tacacatttt ttagttgaaa gttgagagat gctagttaaa tcttgtagta 13081 aaaagtcaca aagaattttt aaaatacata ttaaaattct aatgaagaat tttttcatag 13141 cgctctcaac tctctcctat aaattcttaa ttctttattt tctgatttca attttcaaat 13201 aaaatttact cataaacata atttttttta aacttaataa taatattggt aaagtaaagg 13261 gaggatgtta tttatgaaat ataaattaat atgtatagat atggatggta ccttattaaa 13321 tggtcatagt agtattagtg aaagaaataa agatgcatta aaaaaagctg ttgataaagg 13381 tgtgcaagtt gctataagta ctggaagaat ttttgcttct gctgattatt ttgctgaact 13441 tataggaata aaaacagatt taatttcatg taatggagca tatataaaaa atagatctac 13501 aaatgaagta atatataata atacattaac taatgaacaa gtgttaaaaa ttcataattc 13561 tataaaggga cataatttta gaataatgta ttatactcat gacacagcta ttgttgattc 13621 tccatttcct gaaaatcaca catataattt aacaaataaa ttagttagtg atgaaaaaaa 13681 agttaagttt tttgttacat ctgatataaa taaaattctt gaaatatata gcaataaaat 13741 aatcaaagta atttgcatag atgatagtga tgataaagat actttattta aagttaaaga 13801 taacttatta aagttttctg atatagaaac tgtaagctct ggagataata attttgaaat 13861 tatgcaaaaa gatgtttcaa aaggacatgc tgcaaaaatt ctttctgaaa aacttggcat 13921 aaaaccagag gaagttatat gtataggtga taatgaaaat gacttatcaa tgattaaatt 13981 tgcaggactt ggtgtagcta tggggaatgg ctcagatgtg gttaaaaaaa ttgctaatta 14041 cattacagat acaaatgtaa atgatggtgt tgcaaaagta atagaaaaat ttatacttta 14101 atgtgaattc tttaaattta tctaatttat aactttaatt atacgcaaat tatttaacaa 14161 tataatttgc tcaaaataaa aaaatgtaat aaacgtatct tataccttta ttacatttat 14221 attatcttta acttttggaa tttattgagt tttgttagtt aaagtctttt catttgttta 14281 taatatataa tatgaagtaa ataaataaat gttacataaa attttttaat attaactttt 14341 ataaataaaa ttatttaata gaattttatt tataaaagtt acatttgatt cgaggtgata 14401 attatgaata aaaacaaaca aaaaagaatt gctgttataa atgatttatc tggatttggg 14461 cgttgttctt taactgttgc acttccaata atatctgcta tgaaaataca atgttgccct 14521 ttgcctaccg caattttatc tgcaaataca gcattcccaa atttcttttt tgatgattat 14581 acaaataata tgaaaaattt cacaaatcat tggcaggaga tcaacttaag ttttgatgga 14641 atatgtacag gttttttagg ttctaaaaat caaattgata ttgttataga ttttataaaa 14701 aaatttaaaa ataataatac acacgtttta gttgatccta taatgggcga caacggaaaa 14761 atatattcaa cttacacaga tgaaatgtgt aatgaaatga aatcgctaat aaaatatgct 14821 gatataataa ctcctaatct aactgaagct tgcaagttaa tagatatgcc ctatccaaat 14881 agaattctag atgaaaaaga actagaattt atagctaaaa gtctctgtga aaatggacca 14941 aaaaaaattg taataacagg attacaaaac catgattatg tacagaatta catttatgaa 15001 attggaaaac ctccaaagat aataagtgta aaaaaaattg gaaaagatcg ttgtggtaca 15061 ggggatgtgt ttagttcaat attagctgct gatatggtaa ataatttaga tattatagat 15121 agtgtaaaaa aggctagtga ttttataagt aaatgcctag aatacacatc agaattagac 15181 atttcagtac acgaaggtat atgctttgaa gaatatttat gtgaactaaa ataaaaaagc 15241 gtggataaat ccacgctttt tagttaaaaa ttactagtta aaagtattat aacaatgtgt 15301 actttaaaat tccccaacga aatttttcct taattctcaa ctttccacta tggattctca 15361 actaattttt atatgtattt ttctaaattc atttttattt tttcttttgg taaaaatccc 15421 acttcagtac taacatcttt accgtcttta aatattatca tagttggtat actaaatacg 15481 ctaaacttgc tagctaattc tccgctttca tctacattaa ccttaaagaa tttaacctta 15541 tctttcattt cttcttctaa ttcttcaaat ataggtgcta acatcttaca aggtccacac 15601 caatcagcga aaaagtccac tattacaatt ccttctttta ttgcatcatc gaattcatta 15661 acttttaata tttttgacat aacttattac ctcactttat tctattattt tttataacag 15721 tttgttttta aatatataaa tcttatagag tgtaatcatt cataaccttt aacttagttt 15781 ttaaatatac ttatctttta tttttttcat ctacataaga aactgcagat aatgctgcaa 15841 tatttccttc tcctgctgat ttaatatatt gataaggcac tcctactata tctccagctg 15901 caaagcatcc ttttatattt gtcttcatca atctatcaac tttaacatga ttttgatcta 15961 gttctaatcc tggaaccaat tgaccaggtg atatactatc tcttagtata aaaataccat 16021 ctgtttttaa ttctttatta gataaaatta gagaattaac tttcatatct ccttttatct 16081 caacaggctt atcttttata atttcaatat ctgagtttac ttctacttcc tcattataca 16141 taggtacgta atatacctta gatgctattg atgctataaa attagcttct tcttcatctt 16201 ttttattata agctataata gtcactactt tatctttata taaaggtgca tcacaagttg 16261 cacaatatcc aacacctttt cctaaaaact cttcttcccc attaaatggc tttccaaagt 16321 tcactccagt agctaaaatt atagaagttg cttcatacat cttttcattt accattaatg 16381 aaaaataatc gcccatagca taaatactat ttaccttttc ttcggtaata tttatgtcca 16441 tagattgtaa atgattaaga aactcatctc ttatttctaa gccacttttc ccataaaacc 16501 ctaaataatt atttattgta tgagccttta ctagtttatt actaatatct ttacttccaa 16561 agattataaa attcttcttt cttattcttg catttaatgc tgctgataag cccgcaggac 16621 cacttccaat aatagctatg tcatatctct cacccataaa gttccctcca aatcttaatt 16681 aaaaatatca atctaaaata tattattact aaaaatcaat atttaaatac atttaagtaa 16741 tataaaagct tttatatcta tcttccgtat aagctcatac caactataac ttcacaaaag 16801 atatagttag aattcaattt tactaaattt aatttattaa aatatatttg tttgatttat 16861 tatatatctt tactgttagt aatgtcaaac aaatttaaag ttattataaa aataataatg 16921 gtgcggcata tgatcatttt ttattcttta gatatactca tcataccttt aaaacatggt 16981 attctaaaag ttatccacaa attctttaat aatataaatt tctaaagaat aaaaaaacat 17041 tttattttgt aatatagtaa aattagtatt aaaattactt aaagtaatat atttaaataa 17101 atgttgatat ataatcagta aagttttata aatatgtatc aataatttac taaggaggct 17161 tttttatgat ttattatgac taccatatgc attgcaatta ttcagctgac tcacaggctc 17221 ctatggagga tatgatagaa aaatctattg agttaggact taaagaaatt tgttttactg 17281 atcatgttga ttatgatgta ttaggaaatc caaacgttaa agttgattat gataaatact 17341 ttgaagatat agatttttat aacaacaaat ataaagataa aatatctata aaaaaaggta 17401 ttgaaatggg acttcaaact caagttttag agcgttgttc taaagaaata aaagaaacta 17461 actttgattt tgtcatcgga tctatacata ctatagatcg aaatgaacta tttacaggtg 17521 attttcataa aggaaagacg caacatgagg cttatgaagg atattataaa agcttactag 17581 aaatagtaaa ttcatatgat gattattctg ttttaggaca tgttgatttg ataaaaagat 17641 atggaaatta cgatacaatt ttaaaagatg aaattttttc tgactacttg gaagctatac 17701 taaaaaaagt tatttattca ggtaaaggaa tagaaattaa tacctcatca tttagatata 17761 atcttcctga tttaacacca tctaaaaata ttcttaaaat gtataaagat ttaggtggag 17821 aaatacttac tataggttca gattctcata atccaactca aatagctact aaatttgatt 17881 atattcatga tgctttaggt tctatgggat acaaatatat ttgtaaattc aataacatga 17941 aacctgaatt cataaaacta taatacaaaa aacaaaatca taaatcaaca atataaaata 18001 caaaattata aaacatttaa attttaataa ttatataact tactaaaaaa atcagttata 18061 aagaataata tcttctataa ctgatttttt atattttata ttaaatttcc actaaacgta 18121 attacaatat ataactcctt tgtatctttg taattatata agttaataac aatcttttat 18181 aaagtaaatt ttttaaccat agaattaaga ttatctgaaa cctcagtttc attttccaca 18241 atagtagata tttcatcaac taattgtgaa actagttcta tactagatac aatctctcca 18301 ctattagatg cagtatcttg agaagcttct gcaatatttt gtattgcatc tccaacttca 18361 tgtgtaatgt attcaatgtt atttgtcatc tcagatattt ttatagatat atcttcaata 18421 gcatttgcat ccttatcata ttgattagct attccaacaa attctttata atctggagtt 18481 acagtgtctt taataaataa taacatctgc atagaatcat tagttaattt attaaatgct 18541 tcttgcactt tacttatagt ctcaataatt tcttttaccg tatctgatgt ttgtgttgca 18601 agattaccta tttcagaagc aacaactgca aatccttttc cttgttcacc agcacgtgca 18661 gcttctattg aagcattaag tgataataaa ttaacttgtt ctgctatatc agaaatagct 18721 ttagccatag cccctatatt ttcaactatt ttagcattct caatactttg ctttagattt 18781 gtttcatgaa ctcctgataa actagttgct ttattataag aatcattgct cattttttct 18841 atattgtatg cccttacctt tatgtctttt gccatgtcat gacttttaat tgtttcctgt 18901 gacaataaac ttattgaaga gttaacttct tctacagaag cattaacctc ttcagtggta 18961 gcactagaat taagcataga ttcacttatg ttcttaactg aatctttaat tactttataa 19021 ttttctacta gagattcagt tgatttattt aaattctccg ttatgctatt tactttgttt 19081 gcatcatcag atatttcttg tatcatgttc ttattttctg ataacatttt gtttaatgaa 19141 ttactcattt gtcctaattc atcctcagat attacttcaa gctcatttat agaaaaatct 19201 ccatttgata aatttgatgc taaatcatta acttttttta tagttttagt tagatatcga 19261 acttgccata ttattgctaa tgtagttaat attatggcta aaatccctac cactataagt 19321 ttagacatta attcatttat aggtttattc aattctgaca tcggtatttg tataaacagc 19381 ttccatccaa atggctctac tgtagtataa taagattcat actttgtatc atcaatagaa 19441 aactcacttt taccattagt attatttaac aaaacatttc cgaattctga aagtgattta 19501 ttctcatctt cattaatttt agtttccatt atttttttat catcaatgtt agttatgtat 19561 ttaccatcat tgttaatcaa taatgcctta ccattttcac caattttgaa attatttatc 19621 aaatcctgaa ttgatggaat ttccatatca acactaatta caccaatgaa attatcattt 19681 gaatcatata taggtgtagc gcaagtgctc attatagtat ttaatgtttc atcatagtac 19741 aaatcactaa atacagtttc tttattgtta ttcttagcat tcttatacca atcataatta 19801 aaataattat attcagcatt gctataatca tatgtcacta atgccttatc atcattctta 19861 tgaatataag gtccgacata ttctttactc gcatcataaa catatggttc aaaccaaatt 19921 ccagcaccaa gcaccaaatc acttttaaat attacttttc ctatcatttc ttcatattgt 19981 gataaatatg tagttttata agttgatcca acattcaatg caagttgact agacattgat 20041 gaaatttctt gcattttaag atttattgca tttactgtag aacttaattc tgcttgcatg 20101 ttttcttcca cttgtttgtc tataatattc ttgctacttt tataacttat acccgatagc 20161 aaaaccattg ataaaatagt tacgaacaga agaattgtaa gcattcttgt acttattttt 20221 ttaaatctca t //