LOCUS NC_009699:1 19192 bp DNA linear BCT 03-SEP-2013 DEFINITION Clostridium botulinum F str. Langeland chromosome, complete genome. ACCESSION NC_009699 SOURCE Clostridium botulinum F str. Langeland chromosome, complete genome. COMMENT locus start: 1; locus end: 19192 COMMENT n1_4mer:GRV/n1_4mer:RV = 6.705243; n0_4mer:D = 22.115852; n0_4mer:PS = 42.844236 FEATURES Location/Qualifiers source 1..19192 /organism="Clostridium botulinum F str. Langeland chromosome, complete genome." gene 1..1338 /gene="dnaA" /locus_tag="dnaA" /db_xref="1..1338" CDS 1..1338 /gene="dnaA" /product="chromosomal replication initiation protein" /translation="MSTHLTETWEKAINIIKGELTEVSFNTWIKSINPISLENNSLKL AVPNDFTKGILESRYKDLIVNALKLLTSKKYNIDFIVTTEEKIEKNHNNEKSNIVVND EMSTMLNPKYTFDSFVIGNSNRFAHAASLAVAESPAKAYNPLFIYGGVGLGKTHLMHA IGHYILHNNPKSQVVYVSSEKFTNELINSIKDDKNVEFRNKYRNIDILLVDDIQFIAG KERTQEEFFHTFNALYEANKQIIISSDRPPKEIPTLEDRLRSRFEWGLIADIQAPDFE TRMAILKKKADVEKLNIPNEVMVYIATKIKSNIRELEGALIRIVAFSSLTNKEISVDL ASEALKDIISSKQTRQVTIDIIQEVVANYYNLKIEDLKSARRTRNIAFPRQIAMYLSR KLTDMSLPKIGEEFGGRDHTTVIHAYEKISNNLKKDESLQNAIKELNKRINQK" gene 1601..2704 /gene="dnaN" /locus_tag="dnaN" /db_xref="1601..2704" CDS 1601..2704 /gene="dnaN" /product="DNA polymerase III subunit beta" /translation="MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVL TGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRKLPNDLIEINTLEDNSIEIICQN SRFNLIYMNPDEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTP NHILFSIGETKIISRLLEGEFISYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGN TNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGDDLQIAFNSKYLLDVLKTMEDNEV VLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN" gene 2723..2929 /gene="" /locus_tag="" /db_xref="2723..2929" CDS 2723..2929 /gene="" /product="S4 domain-containing protein" /translation="MVEIKINSEIIKLDSFLKWSGATTLGSEAKFFIQNGEVKVNGEI EKRRGRKLKIDDLIEFNNETYKII" gene 3008..4102 /gene="recF" /locus_tag="recF" /db_xref="3008..4102" CDS 3008..4102 /gene="recF" /product="recombination protein F" /translation="MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIY YSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKI KKISELMGNLNVVMFSPEDLRIIKDYPGNRRKFLDIELCKINNVYYHDLVQYNKILSE RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLED INFRYLTNIKDFDNAEKELLIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFG SQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTII TCTGIEEIDKYLHKKQSQLYLVNNGKIKRV" gene 4123..4386 /gene="" /locus_tag="" /db_xref="4123..4386" CDS 4123..4386 /gene="" /product="hypothetical protein" /translation="MFLHLGENVVVPIKDVIGIFDMETSMYSSDTIQFLRLAEEDGFV ERITKEKPKSFVIAEVDKKSKIYLSPISSATLTKRTKVLYNEL" gene 4404..6317 /gene="gyrB" /locus_tag="gyrB" /db_xref="4404..6317" CDS 4404..6317 /gene="gyrB" /product="DNA gyrase subunit B" /translation="MSQENKQVYDESQIQVLEGLEAVRKRPGMYIGSTSLRGLHHLVY EIVDNSIDEALAGFCTHIEVFIHKDNSITVVDDGRGMPVGMHSKVKKPAVEVIMTILH AGGKFGGGGYKVSGGLHGVGASVVNALSEQCEVEVRREGHIWKQRFERGITKTGLDIV GDTEDHGTKIYFKPDIEIFDELEFEYDTLAQRLRELAFLNKGIKISLTDERYDKKEMF HYEGGLRSFVLYLNRNKEKLHQQPIYVDKNKDGCIVEIAMQYNDGYAENIFSFANNID TIEGGTHLAGFKSALTRAINDYARKFNYLKDTDKNLSGDDVREGLTAVISVKLTDPQF EGQTKTKLGNSEVRGIVDTIVGESIGSFLEENPNVGKIIIEKGLSASRAREAAKRARE LTRRKSVLESTSLPGKLADCSSKDPSLCEIYLVEGDSAGGSAKQGRNREFQAILPLKG KIMNVEKQRLDKILASDEIRAMITAFGAGIGKEFDIEKIRYNRIIIMTDADVDGAHIR TLLLTFFYRYMKELIEEGHVYIAQPPLYRIFKAKKEIYVYSDPELDAALLELGGKDTN TNIQRYKGLGEMNPEQLWDTTMDPEHRTLLQVTIEDAMVADEIFTILMGTKVEPRREF IENNADKVVNLDI" gene 6343..8838 /gene="gyrA" /locus_tag="gyrA" /db_xref="6343..8838" CDS 6343..8838 /gene="gyrA" /product="DNA gyrase subunit A" /translation="MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVH RRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHGDTAVYEALVRMAQDFSIRYTLV DGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS RFPNLLVNGSAGIAVGMATNIPPHNLTEVIDGIIMLIDNEDVNILDLMTKIKGPDFPT FGLIVGTRGIREAYETGRGKVIIRAKAEIEEEKGRNRIVVTEIPYQVNKSKLIENMAN LVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKHTKLQDTFGIIMLAL VNNQPQVLNLKEILVNYVEFQKEVIRRRTKFDLDKALARAHILEGLRIALDHIDEVIK LIRSSKNAAEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISY FREILDKEELVLSIIKEELIEIKNKYGDERKTEIVKGEHDIDIEDLIEDKKVIVTLTH GGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRGKVYKLK AYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEIS KYSSIRKGGLNAINLREDDELIDVKMTKGNNEIIIVTQNGYCIRFNEEDVRPMGRVAT GVKGITLREDDKAVSMEVVLGDEALLSISENGFGKRTDIEEYPIHRRGGKGVITYKIT DKTGPIVGARFVKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVA IAKIKSEDIIEEEILNEEILNEENLNEENLNEE" gene 8935..9051 /gene="" /locus_tag="" /db_xref="8935..9051" CDS 8935..9051 /gene="" /product="hypothetical protein" /translation="MKTIKTEEILSYLNQTEFCVINIILNFDTIKLVLETRV" gene 9253..10754 /gene="rrsA" /locus_tag="rrsA" /db_xref="9253..10754" gene 10912..13813 /gene="rrlA" /locus_tag="rrlA" /db_xref="10912..13813" gene 13907..14023 /gene="rrfA" /locus_tag="rrfA" /db_xref="13907..14023" CDS 13907..14023 /gene="rrfA" /product="5S ribosomal RNA" /translation="SGDYGLKVTPVPMPNTKVKLQSADGTAGEALWKSKSLP" gene 14031..14109 /gene="" /locus_tag="" /db_xref="14031..14109" gene 14032..14108 /gene="" /locus_tag="" /db_xref="14032..14108" gene 14111..14188 /gene="" /locus_tag="" /db_xref="14111..14188" CDS 14111..14188 /gene="" /product="tRNA-Ala" /translation="WGYSSVGRAPALQAGGQEFESPYLH" gene 14112..14187 /gene="" /locus_tag="" /db_xref="14112..14187" CDS 14112..14187 /gene="" /product="tRNA-Ala" /translation="GGIAQLGEHLPCKQGVRSSNLLIST" gene 14382..14804 /gene="" /locus_tag="" /db_xref="14382..14804" CDS 14382..14804 /gene="" /product="hypothetical protein" /translation="MHLDNEELKIFNKLSISEQKHSIKVAYDIEKLYEKGKYNLTKDE FIKVALLHDIGKLNYKVDIIKKSIIVIMDRITNSRIKKFQNIKSVYVHYNHPYLGYCI LKQYNKYSEKMLYLIKNHHNENIINKELSLLIYSDNLN" gene 14820..15341 /gene="" /locus_tag="" /db_xref="14820..15341" CDS 14820..15341 /gene="" /product="transcriptional regulator" /translation="MNSIERRKNIENMLMKNNKPIKGSEMGQKLGVTRQVIVKDIAIL RASGKNIIATPEGYLIPNENKQLIRKIIAVCHDSKDIKDELEVIIKFGGIVEDVVVEH PIYGEIKAMLMIKSMYDIDNFIKSIQNNKAEPLLILTGGIHLHTISSDNEDIMNKIIE ELNQKNYLVDEEV" gene 15343..16506 /gene="" /locus_tag="" /db_xref="15343..16506" CDS 15343..16506 /gene="" /product="(Fe-S)-binding protein" /translation="MDKVALLKCDEYDLEKVEKTIRNGFELLGGNSFLNKLIPYNSKV LLKPNMLSIENEGSPVVTNSVVFEAVIRILRDYSNNISFGDSPGFGDSKKAAEKSGLL NIAKKYNIEFKDFKESINVSCDNSILCRSWTVAKAAYETDVLITLPKLKTHAMAYFTG AVKNQFGCIPGTLKATWHTRMPNANNFCKMLLDLNTLLETDFAILDGIIAMEGNGPKN GTPYNLNTIIMGDSLTAVDSVAVKIIGYDNPLDIPVLKEAYDSNWGSVFLKDIDILGE KIDSMVVDNFKLCREGGDFYFINPKVTNFLKDIIAPDPILIKEKCIGCNRCAEVCPEK PYVIDMIKKGDKKIPVWNMKKCIRCFCCQELCPKGAIETKYSFIGRFLNRDGR" gene 16511..19192 /gene="" /locus_tag="" /db_xref="16511..19192" CDS 16511..19192 /gene="" /product="hypothetical protein" /translation="MKNKKALFIPLFIIILFIAFFNKIINFIINIKWFEEVNYLAVYF TKMRAIIILMIPIFIIFFISIWMYYKSLTINKNKSVVDIGLNKNNYGKKLFFIFNFIV SIFLAYIFSSSYWYRILQFNNSVDFNVKDPIFFKDVSFYIFKLPLFESLYKVIISLLL FLVITTFIAYFILEAKYKIQSRKDINLKNINHGIKSFAGKQLAIVSGLIILFISFGHL IKIWNLVYSSNGVSFGASYTDVHATLLFYKIIVVITLISSIVTLLSIVKGKFKPVSIC IGITIFLIVSQNIASFLVQNFIVKSNEKTLEQPYIKNNIDLTRKAFALDDIEIRDFDI KNDLQKQDIADNKASIDNVRINSFKPTLEFYNQVQIIRYYYTFNDIDIDRYNINGKYN QVFLAAREIDTDALNPNTWQNRHLIYTHGFGAVMNKVNSVTSEGQPDFVIKDIPPYNK TNIKLTNPRIYFGEKTNDYVIVNTKINEFDYPREDSNKTNKYNGHAGIKTSFINRLLF AINKKDINFLLSKDIKKDSKIIINRNIVERAKKIAPFLTYDSDPYMVIYNGKIYWIID AYTTTNRYPYSEPYDSINYIRNSAKVVIDSVDGDTNFYITDKKDPIVNNYAKIFKGLF KEEKDAPKEIREHFRYPKDLFSIQSKVLGKYHVKDPGVFYNGEDLWEVSKDQKHVEGE TNTNDAPYIIMKLPDQNKEEMVLLNYFNVMKKDNMIALFGARMDGEQYGKKILYKLPS DKTVYSPYLFKQKINQDTNISKELSLWNREGSKVQYGDTIILPIKNSLLYIEPLYLRA SGKNSIPEMKRVILSYNDKLVLSSSIQEGIKEIFNSKDNKINDKNEKDSTKTIDDSKL KKAQEYYNKAIEAQKNGDWTKYGENINELGNILNSIK" ORIGIN 1 atgagtaccc accttacaga aacctgggaa aaagcaataa atattataaa aggtgaactt 61 acggaagtaa gttttaatac atggattaaa agtattaacc ctatttctct tgaaaataat 121 tcattgaaac tagctgtacc aaatgatttt acaaaaggaa ttcttgaaag taggtacaaa 181 gatcttatag ttaatgcgct aaaattactt acctcaaaaa aatataatat agattttatt 241 gtaactactg aagaaaaaat agaaaaaaat cataataacg aaaaatctaa tatagttgta 301 aatgatgaaa tgtctactat gttaaatcct aaatatactt ttgattcgtt tgtaatagga 361 aatagtaata gattcgctca tgcagcatct ttagcagtag cagaatcacc tgctaaagca 421 tataatcctt tatttatata tggtggagtt ggtcttggta aaactcactt aatgcatgct 481 atcggtcatt atatattgca taacaatcct aaatctcaag tagtttatgt atcttctgag 541 aaatttacaa atgaattaat taattctata aaagatgata aaaatgtaga atttagaaat 601 aaatatagaa atatagatat tttattagtt gatgatattc aatttattgc tggaaaagaa 661 agaactcaag aggaattttt ccatactttt aatgcactgt atgaagctaa caaacaaata 721 attatatcaa gtgaccgtcc cccaaaagaa atacctactt tagaagatag attaagatct 781 agatttgaat ggggacttat agctgatatt caagcacctg attttgaaac caggatggcc 841 attttaaaga aaaaagctga tgtagaaaaa ttaaatattc ctaatgaagt aatggtatat 901 atagctacaa aaattaaatc aaatattcgt gaattagaag gagctttaat aagaatagta 961 gccttttcat cacttactaa taaagaaata agtgtagatt tagcttctga agcactgaaa 1021 gatataatct caagtaaaca aacaaggcaa gtcactatag atattattca agaagttgta 1081 gcaaactatt ataatctaaa aatagaagat ctaaaatcag ctagaagaac aagaaatatt 1141 gctttcccgc gtcaaatagc catgtacctt tctagaaaac taacggacat gtcattacct 1201 aaaattggtg aagaatttgg cggacgtgat catactacag ttattcatgc ctatgaaaag 1261 atatctaata acttaaaaaa agacgaaagt cttcaaaatg caataaagga attaaataaa 1321 cgaattaatc aaaaataaaa ataatataca aatgtttata ataatatctt atttatttgt 1381 tgataatgta ctaaatataa attaaacata tttcctgtgg ataagcactt aattttaacg 1441 ttaacttatc cacaattatt ttgttatgaa aaatgttgat attttaagct ttgtaagact 1501 tatcaacata tcaacaagcc ctactactac tactactatt ttattatatt atctatttct 1561 attctaacaa taggaattgt taataaagga ggctaatttt atgaaaataa cttgtcaaaa 1621 aaacatttta ctggaaggta tatctatagt tcaaaaagca gtaactggaa aatcaacttt 1681 acctatatta tctggaatac ttattagagc taataaaaat gaattggtct taactggttc 1741 tgatatggat cttagcattg agactaaagt aaaagctaac attttagaag agggaactat 1801 agttttagat gctagacttt ttggtgaaat aataaggaaa ttacctaatg atctaataga 1861 aataaataca ttagaagata attctataga aataatatgt caaaattcta gatttaatct 1921 aatatatatg aatccagatg aatttcctaa tccacctatt atcaatgaaa atatgatatt 1981 ttccataggt gaaagtaaat taaaaaatat gattaaagga actatatttg ctacagctca 2041 agatgaaaca aggcctattc ttacaggtgt tttatttcaa ataaaagata aaatgttaaa 2101 tttagtagct ttagatggtt acaggttagc tttaagaagt gaagttgttg ataatgataa 2161 tacgataaat gctgttattc cagggaaaac attaaatgaa gtttcaaaaa ttttagaaga 2221 agataatgaa aatgttaata ttacatttac tcctaatcat attttattta gcataggaga 2281 aacaaaaata atttcaagat tattagaagg agaatttatt agctataaat ctataatacc 2341 tgaagaattt aacttaaaaa taatagctaa aagatcagag cttttaaatt ctatagaaag 2401 agcctctctt atggctaaag aaggaaacac taatttagtt aaatttgatt tttcagatga 2461 taaaattgta ataacatcaa attctcaatt aggaatggtc cgagaagaat taaaggttgt 2521 attgcaagga gacgatttac aaattgcatt taattcaaaa tatcttttag atgtgttaaa 2581 aactatggaa gataatgagg ttgtattaga attttcaagt agtgtaagtc cttgtattat 2641 aaaaaataca gaaataaaca attgcactta cctagtttta ccagtaagat taaacaataa 2701 ttaattaaaa gaggtattaa aaatggttga aataaaaatt aattcagaaa taataaaatt 2761 agattctttc ttaaaatggt caggggcaac aactttaggt tcagaggcta aattttttat 2821 acaaaacgga gaagttaaag ttaatggaga aatagaaaaa agaagaggta gaaaacttaa 2881 aatagacgac ttaatagagt ttaataatga aacatataaa attatttaaa attatttaaa 2941 attttatata aattttaaat aattgttact aaaaaatatt atggtataat taatttaggt 3001 gtttagaatg tatataaaaa atgtgcactt aataaatttt agaaattatg atgatatgta 3061 tttagaatta agtccaaata caaatatttt tgtaggtaat aatgctcaag ggaaaactaa 3121 tatactagaa agtatatatt attcaagtat aggaaagtct catagaacaa ataaagataa 3181 agatcttata aaatgggata aaaataatac atacttaagg acatatgtat ccagggaaag 3241 attagataaa actatagata taaatatatt taaaaatggc aaaaaagcta taacagtaaa 3301 taaaataaaa ataaaaaaaa tatctgaact aatggggaac ttaaatgttg ttatgttttc 3361 gccagaagac ttaagaataa ttaaggatta ccctggaaat agaaggaaat ttctagatat 3421 agaattatgt aaaataaaca atgtttatta tcatgattta gtccaatata ataaaatttt 3481 atctgaaaga aatacagctt taaaaaattg gaataacaaa attaatgata taattgatat 3541 ttatgatgag caactttcta aatatggagc ttttataatt aaagagagaa ataagtattt 3601 ggataaatta aatattattg gcaaaaatat acataagaag ataaccaatg atttagaaga 3661 cataaatttt agatatttaa ctaatataaa agattttgat aatgcagaaa aagaattatt 3721 aatagttctt aagaaaaatc gaaaaaaaga tcttgagaga aattcaacct ctattggtcc 3781 tcatcgagat gattttgaag taagtataaa caatatagat actagaattt ttggatcaca 3841 aggacaacaa agaacagctg tattaacttt aaagtttgca tcattggaaa taataaaaaa 3901 cataataggg gaatatcctg tgcttttatt agatgatgta ctatctgaat tagattcaaa 3961 tagacagaag tttgtattga attctattga taaaatacaa actataataa cttgtactgg 4021 tatagaagaa atagacaaat acttacataa gaagcaatct caattatatt tagttaataa 4081 tggtaagata aaaagagttt aaagctgaaa ggagattaat ttatgtttct tcacttagga 4141 gaaaatgtag tagtaccgat aaaagatgtt ataggaatat tcgacatgga aacttctatg 4201 tatagttcag atactataca atttttaaga ttagctgaag aggatggatt tgttgaaaga 4261 ataactaaag agaaaccaaa atcttttgtt atagctgaag tagataaaaa gagtaagata 4321 tatttatctc ctatatcttc agctacacta actaaaagaa caaaagtttt gtataatgag 4381 ctgtaaaagt taggaggata aacatgtcac aagaaaataa acaagtttat gatgaaagtc 4441 aaattcaagt gttggaaggt ttagaagctg ttagaaaaag accaggaatg tatattggaa 4501 gcactagttt aaggggactt catcatttgg tttatgaaat agttgataat agcatagatg 4561 aagcactagc tggtttttgt acacatatag aagtttttat acataaagat aattctataa 4621 ctgtggtaga tgatggtcgt ggaatgccag ttggtatgca ttcaaaagta aaaaaacctg 4681 ctgttgaagt aataatgact atattacatg caggtggaaa atttggtggt ggaggatata 4741 aggtttctgg tggacttcat ggtgttggag cttctgttgt taatgcctta tcagaacaat 4801 gtgaagtaga agtaagaaga gaaggccata tttggaaaca aagattcgaa agaggtataa 4861 caaaaactgg attggatata gttggagata cggaagatca tggtacaaaa atatatttta 4921 agccagatat tgaaatattt gatgaattag aatttgaata tgatacttta gctcaaagac 4981 ttagagaatt agccttttta aacaaaggta taaaaatttc attaacagat gaaagatatg 5041 ataaaaaaga aatgtttcac tatgagggcg gattgagatc ttttgtactt tatttaaaca 5101 gaaataagga aaaattacat caacaaccta tatatgttga taaaaataaa gatggatgta 5161 tagttgaaat tgctatgcaa tataacgatg gttatgcaga aaacattttt tcctttgcta 5221 acaatataga cacaatagaa ggtggaactc atttagctgg atttaaatca gctttaacaa 5281 gagctataaa tgactatgct agaaaattta attatttaaa agatacagat aaaaatttat 5341 caggagacga tgttagggaa ggtctaacag cagttatttc agtaaaattg acagatcctc 5401 aatttgaagg acagacaaag acaaagttag gaaatagtga agtaagagga attgtagata 5461 caatagtagg tgaaagcata ggttcattct tagaggaaaa ccctaatgta ggtaaaataa 5521 taattgaaaa aggcttgtca gcctcaagag ctagagaagc tgcaaaaagg gctagggaac 5581 ttactagaag aaaatctgtt ttagaaagta cttctcttcc tggaaaatta gcagattgtt 5641 cttcaaagga tccatcatta tgtgaaattt atttagtcga aggagattct gccggtggat 5701 ctgcaaaaca aggaagaaat agagagttcc aggctattct acctttaaaa ggtaaaataa 5761 tgaatgttga aaagcaaaga ttggataaaa tattagcttc agatgaaata cgtgctatga 5821 taacagcctt tggagcagga ataggaaaag aatttgatat tgagaaaata agatataata 5881 gaataattat aatgacagat gcggatgtag atggagcaca cataagaact ttacttttaa 5941 cattctttta tagatacatg aaagagttaa tagaagaagg tcatgtttat atagctcaac 6001 caccacttta tagaatattt aaggctaaaa aagaaattta tgtttattca gatcctgaat 6061 tggatgctgc attattagaa ttaggtggaa aagatactaa cacaaatatt caaagatata 6121 aaggtcttgg agaaatgaac ccagaacaac tttgggatac tactatggac ccagaacata 6181 gaactttatt acaggtaaca atagaggatg ctatggtagc agatgagata tttactatac 6241 tcatgggaac aaaagtagaa ccaagaagag aatttataga aaataatgct gataaggtag 6301 ttaacttgga tatataagat aagattgtaa agaggtgtaa tcatgctaaa tgaaggaaag 6361 attttgcctg tagatgtaag taaagaaatg aagaaatgtt atatagacta tgccatgagc 6421 gtaatagctg gccgtgcatt accagatgta agagacggat taaagccagt acacagaaga 6481 ataatatatt caatgcaggg attaggatta gctccagaaa aaggttatag aaaatgcgct 6541 agaattgtag gggatgtact aggtaagtat catcctcatg gagatacagc agtttatgaa 6601 gctttagtaa gaatggctca ggatttttca ataaggtata cattagtaga tggacatgga 6661 aactttggtt ctgtggatgg tgatggagca gcggcaatga gatatacaga agcaaagatg 6721 agcaaaatat ccatggaact tataaaggat ataaataaaa atacagttga ttttattcct 6781 aactttgatg gtgaagaaga agaaccatct gtattaccat caagatttcc taatctttta 6841 gttaatggat ctgcaggtat agcagtaggt atggctacta atattccacc tcataattta 6901 acagaggtta tagatggaat aataatgcta atagataatg aagatgtaaa tatacttgac 6961 ttaatgacta aaataaaagg tccagatttc cctacttttg gattaatcgt aggtacaaga 7021 ggaataagag aagcttatga aacaggtaga ggaaaagtta taattagagc aaaagctgaa 7081 attgaggaag aaaaaggtag gaatagaata gtagttacag aaataccata ccaggttaac 7141 aaatctaagc ttatagaaaa tatggctaac cttgtaaaag ataaaaagat aaatggaatt 7201 tcagatctaa gagatgagtc agatagagat ggtatgagaa ttgttataga attaaaaagg 7261 gatgcaaatc ctaatatagt tttaaatcaa ttatataagc ataccaaact tcaagatacc 7321 tttgggataa ttatgttagc tctagttaat aatcaacctc aagttttaaa tttaaaagaa 7381 atattagtta attatgtgga atttcaaaag gaagtaataa gaagaagaac taaatttgac 7441 ttagataaag cattagcaag ggctcatata ttagaaggtc taagaatagc tttagatcac 7501 attgatgaag tcataaagct tataagatca tctaaaaatg cagctgaagc taaagaggga 7561 ttaatgaata actttaatct ttcagaaaaa caagctcaag ccatattaga tatgaagtta 7621 caaagactta caggtttaga aagagaaaaa atagaagaag aatataaaga gcttatggaa 7681 aagataagtt attttagaga aatattagat aaagaagaat tagtgctaag tataataaaa 7741 gaagaattaa tagaaataaa aaataaatat ggcgatgaaa gaaaaacaga aatagtaaaa 7801 ggtgagcatg atatagatat agaggactta attgaagata agaaggtaat agtaacttta 7861 actcatggtg gatacataaa aagattagat atggatacat actcctcaca aaaaagagga 7921 ggaaagggta ttcaagctac atctacaaaa caggatgatt ttatagaaaa tatgtttgta 7981 acatctactc atagtactat attattcttc acaaataggg gtaaggtata taaacttaaa 8041 gcttatgaaa taccagaagc aggaagaaca gctaaaggta caaatatagt aaatattata 8101 cctatagaaa ataatgagaa gatacaaacg gtaataggtt taaaagacat agatgatatg 8161 aagcattttg taatgtgtac tagaaatgga ataattaaaa agacagagat aagtaaatat 8221 tcttccataa gaaaaggcgg gttaaatgcc ataaatttaa gagaagatga tgaattaata 8281 gatgtaaaaa tgactaaagg aaataatgaa ataataatag ttacacaaaa tgggtactgc 8341 ataagattta atgaagaaga tgtaaggcct atgggaagag ttgcaacagg agttaagggt 8401 ataacattaa gagaagatga taaagctgta tctatggagg tagtgttagg agatgaagca 8461 ctactatcaa taagtgaaaa tggatttgga aaaagaacag atatagaaga atatcctatc 8521 catagaagag gtggaaaagg agttataact tataagataa ctgacaaaac aggcccaata 8581 gttggagcaa ggtttgtaaa agaagatgat gaacttatgc tagtaaatag cggtgatgtt 8641 gcaataagaa taaatgtttc agaaatatct aaaactagta gaaacgccat gggtgtaaaa 8701 ttaatgagaa ctagtgaaga agaaaagata gtagctatag ctaaaataaa gagtgaagat 8761 ataatagaag aagaaatttt aaatgaagaa attttaaatg aagagaactt aaatgaagag 8821 aacttaaatg aagaataaat aataagaaaa atcagcatta atgctgattt ttcttattat 8881 gaattttaat tataaaaaac aacttttcca gacgtatcaa tataagaaat ataaatgaaa 8941 accataaaaa cagaagaaat attatcatat ttaaatcaaa cagagttttg tgttataaat 9001 ataatattaa attttgatac aataaaactc gtccttgaga caagggtttg aaagtaacaa 9061 aaactttcaa aacaaatttg aaaaaagttc ttgacaaaga acaacaaatt tgataagata 9121 taaaagtcgc tgaggcgatg tggtctttga aaattaaaca gagaattaac aagaaacatt 9181 cttgtaacag ccagtaagat aaggtaataa accttgtcaa tgaatttgag aagagattaa 9241 acttttaaat ttagagtttg atcctggctc aggacgaacg ctggcggcgt gcttaacaca 9301 tgcaagtcga gcgatgaagc ttccttcggg aagtggatta gcggcggacg ggtgagtaac 9361 acgtgggtaa cctgcctcaa agtgggggat agccttccga aaggaagatt aataccgcat 9421 aatataagag aatcgcatga ttttcttatc aaagatttat tgctttgaga tggacccgcg 9481 gcgcattagc tagttggtaa ggtaacggct taccaaggca acgatgcgta gccgacctga 9541 gagggtgatc ggccacattg gaactgagac acggtccaga ctcctacggg aggcagcagt 9601 ggggaatatt gcgcaatggg ggaaaccctg acgcagcaac gccgcgtggg tgatgaaggt 9661 cttcggattg taaagccctg ttttctagga cgataatgac ggtactagag gaggaagcca 9721 cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcgagcgttg tccggattta 9781 ctgggcgtaa agggtgcgta ggcggatgtt taagtgggat gtgaaatccc cgggcttaac 9841 ctgggggctg cattccaaac tggatatcta gagtgcagga gaggaaagcg gaattcctag 9901 tgtagcggtg aaatgcgtag agattaggaa gaacaccagt ggcgaaggcg gctttctgga 9961 ctgtaactga cgctgaggca cgaaagcgtg ggtagcaaac aggattagat accctggtag 10021 tccacgccgt aaacgatgga tactaggtgt agggggtatc aactccccct gtgccgcagt 10081 taacacaata agtatcccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg 10141 acgggggccc gcacaagcag cggagcatgt ggtttaattc gaagcaacgc gaagaacctt 10201 acctggactt gacatccctt gcatagccta gagataggtg aagcccttcg gggcaaggag 10261 acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttaggttaa gtcctgcaac 10321 gagcgcaacc cttgttatta gttgctacca ttaagttgag cactctaatg agactgcctg 10381 ggtaaccagg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg tccagggcta 10441 cacacgtgct acaatggtag gtacaataag acgcaagacc gtgaggtgga gcaaaactta 10501 taaaacctat ctcagttcgg attgtaggct gcaactcgcc tacatgaagc tggagttgct 10561 agtaatcgcg aatcagaatg tcgcggtgaa tacgttcccg ggccttgtac acaccgcccg 10621 tcacaccatg agagctggta acacccgaag tccgtgaggt aaccgtaagg aaccagcggc 10681 cgaaggtggg attagtgatt ggggtgaagt cgtaacaagg tagccgtagg agaacctgcg 10741 gctggatcac ctcctttcta aggagaatag aaagaagaaa attctttcta aaggctgaat 10801 tctctgttta attttgagag accaagagtt tctcaaaatg ttctttgaaa attgcacagt 10861 gaataagtaa agctaaaggt ataaaaatcc tttgtaagaa tacaatttta aggtcaagct 10921 acaaagggcg catggtgaat gccttggcac taggagccga agaaggacgc gataagctgc 10981 gataagcctt gggtagccgc aaataggctg agatccaggg atttccgaat gaggaaactc 11041 acatgggtaa ccccatgtat tatgcactga atacataggt gtataagggt aaacccgggg 11101 aactgaaaca tctaagtacc cggaggaaga gaaagaaaaa tcgatttcct aagtagcggc 11161 gagcgaacgg gaaagagccc aaaccagaaa cttgtttctg gggttgtgga tagatcataa 11221 aagaagaggt atcttaatcg aagagggctg gaacgcccta ccatagaagg taatagtcct 11281 gtagataaaa agagaaaact tcgagatcta atccagagta ccacgagaca cgtgaaacct 11341 tgtgggaagc agggaggacc acctcccaag gctaaatact acctagtgac cgatagtgaa 11401 gcagtaccgt gagggaaagg tgaaaagaac cccggaaggg gagtgaaata gaacctgaaa 11461 ccgtgtgcct acaaccgatc ggagcacgtt aaagtgtgac gatgtgcttt ttgtagaacg 11521 agccagcgag ttacgctatg tagcaaggtt aagtacttaa ggtatggagc cgaagggaaa 11581 ccgagtctga aaagggcgaa aagttgcatg gtgtagaccc gaaaccgggt gacctatcca 11641 tggccaggtt gaagcgagag taaaatctcg tggaggaccg aaccacgttg gtgttgaaaa 11701 accatgggat gagctgtgga tagcggagaa attccaatcg aactcggaga tagctggttc 11761 tcctcgaaat agctttaggg ctagcgtcgt gtaattgagt aatggaggta gagcactgaa 11821 tgggctaggg gctatagtag ttaccgaacc ctatcaaact ccgaatgcca tatacttgta 11881 tcacggcagt cagactgcga atgataagat ccgtagtcaa aagggaaaca gcccagacca 11941 tcagctaagg tcccaaagtg taagttaagt ggaaaaggat gtgggatttc taagacaact 12001 aggatgttgg cttagaagca gccactcatt taaagagtgc gtaatagctc actagtcaag 12061 agatcctgcg ccgaagatgt ccggggctca aacttaccac cgaagctatg ggtgtacact 12121 atgtgtacgc ggtagaggag ctttctgtat gggttgaagt cgtaccgtaa ggagcggtgg 12181 actgtacaga agtgagaatg ctggcataag tagcgagaaa taagtgagaa tcttattggc 12241 cgaaaaccta aggtttcctg aggaaggttc gtccgctcag ggttagtcgg gacctaagcc 12301 gaggccgaaa ggcgtaggtg atggacaatc ggttgatatt ccgataccac ctatttacgt 12361 ttgagaaatg gggtgacgca gtaggataag atgtgcgcac tattggatgt gcgtctaagc 12421 acttaggcat gcttgatagg caaatccgtc gagctaagct gaggtgtgat ggggagccat 12481 ttatggcgaa gtatctgatt ccacactgcc aagaaaagcc tctatcgagt gaataggtgc 12541 ccgtaccgca aaccgacaca ggtaggtgag gagagaatcc taaggccatc ggaagaattg 12601 ctgttaagga actcggcaaa ttgaccccgt aacttcggga gaaggggtgc ctacgaaagt 12661 aggccgcaga gaataggccc aagcaactgt ttagcaaaaa cacaggtctc tgctaaagcg 12721 aaagctgatg tataggggct gacgcctgcc cggtgctgga aggttaaggg gaacacttag 12781 cgtaagcgaa ggtgtgaact taagccccag taaacggcgg ccgtaactat aacggtccta 12841 aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaatggcgta atgatttggg 12901 cactgtctca acagcaaatc cggcgaaatt gtagtgcaag tgaagatgct tgctacccgc 12961 gattggacgg aaagaccccg tagagcttta ctgtagctta gcattgaatc tcggtattgt 13021 ctgtacagga taggtgggag acttggaagc ttgggcgtca gcctgagtgg agtcatcctt 13081 gggataccac cctgacagta ctggggttct aaccggcggc catgaatctg gtcacgggac 13141 attgttaggt gggcagtttg actggggcgg tcgcctccta aaaagtaacg gaggcgccca 13201 aaagttccct cagcgcggtc ggaaatcgcg cgaagagtgc aaaggcagaa gggagcttga 13261 ctgcgacaca tacaggtgga gcagggacga aagtcgggct tagtgatccg gtggtacctc 13321 gtgggagggc catcgctcaa cggataaaag ctacctcggg gataacaggc tgatctcccc 13381 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg 13441 ctgaagtagg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc 13501 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gtaggaaatt tgagaggagc 13561 tgtccttagt acgagaggac cgggatggac caacctctgg tgcaccagtt gtcacgccag 13621 tggcacagct gggtagctat gttgggactg gataaacgct gaaagcatct aagcgtgaag 13681 ccaacctcaa gatgagattt cccatagcgt aagctagtaa gactcctgga agaacaccag 13741 gttgataggt cagagatgta agcatggcaa catgttaagt tgactgatac taataagtcg 13801 agggcttgac caaaataaaa ttcactgtgt aattttgaga gaaacaataa aaaaattgtt 13861 aaatctctat tgacaaattg aaaaaaagca attataatgt taaatatctg gtgattatgg 13921 cttgaaggta acacccgttc ccatgccgaa cacgaaggtt aagcttcaaa gcgctgatgg 13981 tactgcaggg gaagccctgt ggaagagtaa gtcgttgcca ggtgaaaaca tggatcttta 14041 gctcagttgg ttagagcaac cggctcataa ccggtaggtc cggggttcga gtccctgaag 14101 gtccaccatt tgggggtata gctcagttgg gagagcacct gccttgcaag cagggggtca 14161 ggagttcgaa tctccttatc tccaccataa atataagata cttatgaatt ttcataggta 14221 tcttattttt ttatatccat ttaaaaaaca agaaatttat tgtaaactaa taatataaga 14281 ttattttaat agggaggttt aataaaatag tatatagaat taaacaattt cactggaacg 14341 ctactgctaa attggaatat gaagacatag tatatataaa tatgcactta gataatgagg 14401 aattaaagat ttttaataaa ttatctataa gcgaacaaaa gcattctata aaggtagctt 14461 atgatataga aaagttatat gaaaagggta aatataatct aacaaaagat gagtttataa 14521 aggtagcttt actacacgat ataggaaaat taaattataa agttgatatt ataaaaaaaa 14581 gcataatagt aattatggat agaattacaa attccagaat aaaaaaattt caaaatatta 14641 agtcagttta tgttcattat aatcatcctt atttaggtta ttgtatttta aaacaatata 14701 ataaatatag tgaaaaaatg ttatatttaa ttaaaaacca tcataatgaa aatattataa 14761 ataaagagtt aagcttatta atatatagtg ataatttaaa ttaataaggt ggtgatatta 14821 tgaattcaat agaaagaaga aaaaatattg aaaatatgtt aatgaaaaat aataaaccta 14881 ttaaaggtag tgaaatggga caaaaattag gtgtaacaag gcaggttata gtaaaagata 14941 tagctatttt aagagcttct ggtaaaaata taattgctac gcctgaaggt tatttaatac 15001 ctaatgaaaa taaacaatta ataaggaaaa ttatagctgt atgccatgat agtaaagata 15061 taaaagacga attagaggtt ataattaaat ttggaggaat agtagaagat gttgtagtag 15121 aacatcctat atatggagaa ataaaagcca tgttgatgat aaaatctatg tatgacatag 15181 acaattttat aaaaagtata caaaacaata aggcggaacc gttattaata cttactggtg 15241 gaattcattt gcatacaata tcttcagata atgaagatat aatgaataaa ataatagaag 15301 aattaaacca aaaaaattat ttagtagatg aggaagtata aaatggataa agtagccctt 15361 ttaaaatgtg atgaatatga tttagaaaag gtagagaaaa ctataagaaa tggatttgaa 15421 cttttaggag gaaactcttt tttgaataaa cttatacctt ataatagtaa agttctttta 15481 aaacctaaca tgcttagtat tgaaaatgaa ggttctcctg tggtaactaa tagtgtagtt 15541 tttgaagcag taataagaat attaagagat tattctaata acatatcctt tggagattct 15601 cctggatttg gagattctaa aaaggcagct gaaaaatctg gattacttaa tatagcaaag 15661 aaatataata tagaatttaa agattttaaa gaatctatta atgtaagttg tgataattct 15721 attttatgca gatcctggac tgtagcgaag gcagcttatg aaacagatgt actaataaca 15781 ttacctaaat taaagacaca tgctatggct tactttacag gtgcagttaa gaatcagttt 15841 ggatgtatac caggaacttt aaaagctact tggcatacaa gaatgcctaa tgcaaataat 15901 ttctgtaaaa tgttattaga tctaaataca ttgttagaaa ctgattttgc tatactagat 15961 ggcattatag ctatggaagg aaatggtcct aaaaatggaa ctccttataa tcttaataca 16021 attataatgg gggatagcct tacagcggta gattccgttg ctgttaaaat aataggatat 16081 gacaatccat tagatatacc tgtattaaaa gaagcttatg atagcaattg gggaagtgtt 16141 tttttaaagg acatagatat attaggtgaa aaaatagata gcatggtcgt agataatttt 16201 aaactttgta gagagggtgg ggatttttat tttataaatc ctaaagttac aaatttttta 16261 aaggatatta ttgcaccaga tcctatttta ataaaagaaa agtgtatagg atgcaataga 16321 tgtgctgaag tatgtcctga aaaaccatat gttatagata tgataaaaaa aggggataaa 16381 aaaataccag tttggaatat gaaaaaatgt ataagatgtt tttgttgtca ggaactttgt 16441 cccaaaggag ctatagaaac taaatattct tttattggaa gatttttaaa tagagatggt 16501 aggtgataaa atgaaaaata aaaaagcatt gtttatacca ttattcataa taattttatt 16561 tatagcattt tttaataaaa taataaattt tattattaat ataaaatggt ttgaagaagt 16621 aaactattta gcggtatatt ttactaaaat gagagctata attattttaa tgattcctat 16681 atttataata ttttttatat ctatatggat gtattataaa agccttacta taaataagaa 16741 taaaagtgta gtagatatag gattaaataa aaacaattat gggaaaaaat tattctttat 16801 ttttaacttt atagtatcta tatttttagc ttatatattt tcaagcagtt attggtatag 16861 aatattacaa tttaataatt ctgtagattt taatgtgaaa gatcctatct tttttaaaga 16921 tgtttctttt tatatattta aattgccact ttttgaatct ttatataaag ttataatttc 16981 tttattatta tttttagtta taacaacatt tatagcttat tttattttag aggctaaata 17041 taaaattcaa agtagaaagg atataaattt aaaaaatata aatcatggca taaaatcctt 17101 tgcaggaaaa caattagcta ttgtatctgg tcttataatt ctatttatat cctttggaca 17161 tttaataaaa atatggaatt tagtttatag ttctaatgga gtatcttttg gtgctagcta 17221 tactgatgtt cacgctactt tattatttta taaaataata gtagtaatta cactaatttc 17281 atctatagta acattattaa gtatagtaaa aggtaaattt aagccagtaa gcatatgtat 17341 aggtataact atatttttga tagtatcaca aaatatagca tcttttttag tacaaaactt 17401 tatagtcaaa tctaatgaaa aaactttaga gcagccatat ataaaaaata atatagattt 17461 aacaagaaaa gcttttgcat tagatgatat tgaaataaga gattttgata ttaaaaatga 17521 tttacaaaag caagatattg cagacaacaa agcaagtata gataacgtaa gaataaattc 17581 ttttaaacct acattagaat tttataatca agttcaaata ataagatatt actatacttt 17641 taatgacata gatatagata ggtataatat aaatggaaaa tacaatcaag tattcctagc 17701 tgcaagagaa atagatacag atgcattaaa tccaaataca tggcaaaata gacacttaat 17761 atatacacat ggttttggag cagttatgaa taaagtaaat tctgtaacaa gtgaaggaca 17821 acctgacttt gttataaaag atataccacc ttataataaa acaaatataa aattgactaa 17881 tcctaggata tattttggag aaaaaactaa tgactatgtt atagtaaaca ctaagataaa 17941 tgaatttgat tatcctagag aagattctaa taaaactaat aaatataatg gacatgcagg 18001 tataaaaacg agctttatta atagattatt atttgctata aataaaaaag atattaattt 18061 tcttttatca aaggatataa aaaaagatag taaaataata ataaatagaa atattgtaga 18121 gagggcaaaa aaaatagcac cctttttaac ttatgatagt gatccttata tggttatata 18181 taatggcaaa atatattgga taatagatgc ttatactact acaaatagat atccttattc 18241 agagccttac gatagtataa attatataag gaattcagct aaagtagtaa tagattctgt 18301 agatggagat accaattttt atattacaga taaaaaggat cctatagtta ataattatgc 18361 taaaatattt aagggattat ttaaagaaga aaaagatgcg cctaaagaaa taagagaaca 18421 ttttagatat ccaaaagatt tatttagtat tcaaagcaaa gtattaggaa aatatcatgt 18481 aaaagatcct ggtgtgtttt ataatggtga agatctatgg gaagtgtcta aggatcaaaa 18541 acatgttgaa ggagaaacaa atacaaatga tgctccttat ataattatga aattaccaga 18601 ccaaaataaa gaagaaatgg tacttttgaa ttattttaat gtaatgaaaa aggataatat 18661 gatagcttta tttggagcaa gaatggatgg agagcaatat ggtaaaaaaa tattatataa 18721 acttccatca gacaaaacag tatatagtcc atatttattt aagcaaaaaa taaatcaaga 18781 tactaatata tctaaggaat tatcattatg gaatagagaa ggttcaaagg tgcaatatgg 18841 agacactata atattaccta taaaaaactc tcttctttat atagaaccac tatatttgcg 18901 agctagtggg aaaaatagta taccagaaat gaaaagagta atattatcct ataatgataa 18961 attagtttta tcatctagta ttcaagaagg tataaaagaa atatttaatt ctaaagataa 19021 taaaatcaat gataaaaatg aaaaagatag tacaaagact atagatgatt caaaattgaa 19081 aaaagctcaa gaatattata ataaagccat agaagctcaa aaaaatggag attggactaa 19141 atatggagaa aatataaatg aattaggtaa tatattaaat agtataaaat aa //