Pre_GI: BLASTP Hits

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Query: NC_020409:363114:386605 Desulfovibrio piezophilus str. nov C1TLV30 chromosome, complete

Start: 386605, End: 387594, Length: 990

Host Lineage: Desulfovibrio piezophilus; Desulfovibrio; Desulfovibrionaceae; Desulfovibrionales; Proteobacteria; Bacteria

General Information: Desulfovibrio piezophilus sp. nov. C1TLV30 is able to grow up to 20 MPa. Pressure growth conditions. Nitrogen fixation, Dissimilatory sulfate reduction. Sulfate-reducing, piezophilic and anaerobic bacterium. Isolated from Mediterranean deep-sea falls at a depth of 1700 m




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SubjectStartEndLengthSubject Host DescriptionCDS descriptionE-valueBit score
NC_016629:3308329:331179233117923312781990Desulfovibrio africanus str. Walvis Bay chromosome, completeketol-acid reductoisomerase8e-144509
NC_019978:224619:244408244408245400993Halobacteroides halobius DSM 5150, complete genomeketol-acid reductoisomerase5e-119427
NC_005125:2811986:281722328172232818221999Gloeobacter violaceus PCC 7421, complete genomeketol-acid reductoisomerase3e-117421
NC_007604:1586243:160937316093731610356984Synechococcus elongatus PCC 7942, complete genomeketol-acid reductoisomerase9e-117419
NC_006576:2683467:103431034311335993Synechococcus elongatus PCC 6301, complete genomeketol-acid reductoisomerase2e-116419
NC_018012:1076839:1084631108463110856531023Thiocystis violascens DSM 198 chromosome, complete genomeketol-acid reductoisomerase1e-115416
NC_014328:2203437:2221957222195722229701014Clostridium ljungdahlii ATCC 49587 chromosome, complete genomeketol-acid reductoisomerase2e-114412
NC_014323:4355266:4358635435863543596511017Herbaspirillum seropedicae SmR1 chromosome, complete genomeketol-acid reductoisomerase oxidoreductase2e-114412
NC_014328:2203437:2220565222056522215781014Clostridium ljungdahlii ATCC 49587 chromosome, complete genomeketol-acid reductoisomerase2e-114412
NC_007516:774489:808492808492809487996Synechococcus sp. CC9605, complete genomeketol-acid reductoisomerase1e-113409
NC_020291:270234:2858052858052868091005Clostridium saccharoperbutylacetonicum N1-4(HMT), complete genomeketol-acid reductoisomerase IlvC4e-113408
NC_014654:1581689:159116815911681592154987Halanaerobium sp. 'sapolanicus' chromosome, complete genomeketol-acid reductoisomerase8e-113407
NC_015222:2136657:2157495215749521585111017Nitrosomonas sp. AL212 chromosome, complete genomeketol-acid reductoisomerase6e-113407
NC_015738:1971211:198871919887191989714996Eggerthella sp. YY7918, complete genomeketol-acid reductoisomerase4e-113407
NC_010003:1724500:173363417336341734626993Petrotoga mobilis SJ95, complete genomeketol-acid reductoisomerase4e-113407
NC_009617:234782:2474722474722484761005Clostridium beijerinckii NCIMB 8052 chromosome, complete genomeketol-acid reductoisomerase2e-112405
NC_014153:2282725:2296272229627222972881017Thiomonas intermedia K12 chromosome, complete genomeketol-acid reductoisomerase3e-112404
NC_014314:576465:6369916369916379951005Dehalogenimonas lykanthroporepellens BL-DC-9 chromosome, completeketol-acid reductoisomerase7e-112404
NC_000853:569576:5768585768585778681011Thermotoga maritima MSB8, complete genomeketol-acid reductoisomerase9e-112403
NC_010483:363525:3756603756603766701011Thermotoga sp. RQ2, complete genomeketol-acid reductoisomerase9e-112403
NC_013642:323799:3310813310813320911011Thermotoga naphthophila RKU-10, complete genomeketol-acid reductoisomerase1e-111403
NC_016584:3645245:365718036571803658178999Desulfosporosinus orientis DSM 765 chromosome, complete genomeketol-acid reductoisomerase3e-111402
NC_014410:8694:131491314914144996Thermoanaerobacterium thermosaccharolyticum DSM 571 chromosome,ketol-acid reductoisomerase2e-111402
NC_014829:1574739:1582820158282015838511032Bacillus cellulosilyticus DSM 2522 chromosome, complete genomeketol-acid reductoisomerase1e-111402
NC_015958:11511:160291602917024996Thermoanaerobacter wiegelii Rt8.B1 chromosome, complete genomeKetol-acid reductoisomerase3e-111401
NC_011969:1816746:1828159182815918291661008Bacillus cereus Q1 chromosome, complete genomeketol-acid reductoisomerase3e-111401
NC_019970:8938:133751337514370996Thermoanaerobacterium thermosaccharolyticum M0795, complete genomeketol-acid reductoisomerase6e-111400
NC_009486:346665:3576223576223586321011Thermotoga petrophila RKU-1 chromosome, complete genomeketol-acid reductoisomerase8e-111400
NC_014393:1590893:1628382162838216293861005Clostridium cellulovorans 743B chromosome, complete genomeketol-acid reductoisomerase1e-110399
NC_021182:2219286:2241146224114622421501005Clostridium pasteurianum BC1, complete genomeketol-acid reductoisomerase1e-110399
NC_003030:95918:99393993931004061014Clostridium acetobutylicum ATCC 824, complete genomeketol-acid reductoisomerase4e-110398
NC_015687:95918:99393993931004061014Clostridium acetobutylicum DSM 1731 chromosome, complete genomeketol-acid reductoisomerase4e-110398
NC_017295:95919:99394993941004071014Clostridium acetobutylicum EA 2018 chromosome, complete genomeketol-acid reductoisomerase4e-110398
NC_016077:113246:1286591286591296721014Acidaminococcus intestini RyC-MR95 chromosome, complete genomeketol-acid reductoisomerase7e-110397
NC_019815:114288:1232821232821242981017Candidatus Kinetoplastibacterium crithidii (ex Angomonas deaneiketol-acid reductoisomerase8e-110397
NC_016779:1688946:1703744170374417047511008Bacillus cereus F837/76 chromosome, complete genomeketol-acid reductoisomerase1e-109396
NC_008600:1396471:1402701140270114037171017Bacillus thuringiensis str. Al Hakam, complete genomeketol-acid reductoisomerase2e-109395
NC_006274:1367377:1377258137725813782681011Bacillus cereus E33L, complete genomeketol-acid reductoisomerase2e-109395
NC_016779:1321723:1329337132933713303471011Bacillus cereus F837/76 chromosome, complete genomeketol-acid reductoisomerase2e-109395
NC_021184:569653:584846584846585841996Desulfotomaculum gibsoniae DSM 7213, complete genomeketol-acid reductoisomerase2e-109395
NC_015555:4478:131011310114096996Thermoanaerobacterium xylanolyticum LX-11 chromosome, completeKetol-acid reductoisomerase5e-109394
NC_012472:1339500:1345599134559913466091011Bacillus cereus 03BB102, complete genomeketol-acid reductoisomerase4e-109394
NC_003869:14153:181601816019155996Thermoanaerobacter tengcongensis MB4, complete genomeketol-acid reductoisomerase4e-109394
NC_019757:4953806:495476349547634955758996Cylindrospermum stagnale PCC 7417, complete genomeketol-acid reductoisomerase3e-109394
NC_018867:993903:101491310149131015908996Dehalobacter sp. CF chromosome, complete genomeKetol-acid reductoisomerase8e-109393
NC_014335:1329414:1339310133931013403201011Bacillus cereus biovar anthracis str. CI chromosome, completeketol-acid reductoisomerase1e-108393
NC_008786:1936626:1945330194533019463731044Verminephrobacter eiseniae EF01-2, complete genomeketol-acid reductoisomerase2e-108392
NC_011658:1437813:1447420144742014484301011Bacillus cereus AH187 chromosome, complete genomeketol-acid reductoisomerase1e-108392
NC_011969:1437514:1447121144712114481311011Bacillus cereus Q1 chromosome, complete genomeketol-acid reductoisomerase1e-108392
NC_016771:1397292:1406899140689914079091011Bacillus cereus NC7401, complete genomeketol-acid reductoisomerase1e-108392
NC_017208:1380870:1387495138749513885051011Bacillus thuringiensis serovar chinensis CT-43 chromosome, completeketol-acid reductoisomerase3e-108391
NC_010184:1373375:1380898138089813819081011Bacillus weihenstephanensis KBAB4, complete genomeketol-acid reductoisomerase4e-108391
NC_011773:1402155:1408238140823814092481011Bacillus cereus AH820 chromosome, complete genomeketol-acid reductoisomerase4e-108391
NC_013161:1023716:104603910460391047034996Cyanothece sp. PCC 8802, complete genomeketol-acid reductoisomerase2e-107389
NC_011772:1329730:1337117133711713381271011Bacillus cereus G9842, complete genomeketol-acid reductoisomerase1e-107389
NC_003997:1334000:1342535134253513435451011Bacillus anthracis str. Ames, complete genomeketol-acid reductoisomerase1e-107389
NC_007530:1332768:1342658134265813436681011Bacillus anthracis str. 'Ames Ancestor', complete genomeketol-acid reductoisomerase1e-107389
NC_005945:1334000:1342604134260413436141011Bacillus anthracis str. Sterne, complete genomeketol-acid reductoisomerase1e-107389
NC_012581:2885519:2895527289552728965371011Bacillus anthracis str. CDC 684 chromosome, complete genomeketol-acid reductoisomerase1e-107389
NC_012659:1334000:1342558134255813435681011Bacillus anthracis str. A0248, complete genomeketol-acid reductoisomerase1e-107389
NC_014171:1356000:1365500136550013665101011Bacillus thuringiensis BMB171 chromosome, complete genomeketol-acid reductoisomerase1e-107389
NC_011898:3973627:399913639991364000131996Clostridium cellulolyticum H10, complete genomeketol-acid reductoisomerase1e-107389
NC_004722:1352000:1361906136190613629221017Bacillus cereus ATCC 14579, complete genomeketol-acid reductoisomerase3e-107388
NC_011725:1383798:1393702139370213947121011Bacillus cereus B4264 chromosome, complete genomeketol-acid reductoisomerase3e-107388
NC_011726:1023690:104601310460131047008996Cyanothece sp. PCC 8801, complete genomeketol-acid reductoisomerase3e-107388
NC_013517:104480:1237311237311248101080Sebaldella termitidis ATCC 33386, complete genomeketol-acid reductoisomerase3e-107388
NC_012121:1578190:1578720157872015797241005Staphylococcus carnosus subsp. carnosus TM300, complete genomealpha-keto-beta-hydroxylacil reductoisomerase2e-107388
NC_013939:1688400:1696602169660216976181017Deferribacter desulfuricans SSM1, complete genomeketol-acid reductoisomerase4e-107387
NC_016633:1130705:114288611428861143878993Sphaerochaeta pleomorpha str. Grapes chromosome, complete genomeketol-acid reductoisomerase7e-107387
NC_018604:1263927:126932612693261270321996Brachyspira pilosicoli WesB complete genomeketol acid reductoisomerase3e-105382
NC_019908:436000:452771452771453766996Brachyspira pilosicoli P43/6/78 chromosome, complete genomeketol acid reductoisomerase2e-105382
NC_011365:1969198:1973686197368619747051020Gluconacetobacter diazotrophicus PAl 5 chromosome, complete genomeketol-acid reductoisomerase2e-105382
NC_014330:195447:204570204570205565996Brachyspira pilosicoli 95/1000 chromosome, complete genomeketol acid reductoisomerase2e-105382
NC_005835:823187:8269288269288279411014Thermus thermophilus HB27, complete genomeketol-acid reductoisomerase1e-105382
NC_018607:1505908:153519415351941536189996Brachyspira pilosicoli B2904 chromosome, complete genomeketol acid reductoisomerase4e-105381
NC_017243:2383577:239342123934212394416996Brachyspira intermedia PWS/A chromosome, complete genomeketol-acid reductoisomerase8e-105380
NC_012225:1213421:122434312243431225338996Brachyspira hyodysenteriae WA1, complete genomeketol-acid reductoisomerase8e-105380
NC_010723:298954:3116503116503126661017Clostridium botulinum E3 str. Alaska E43, complete genomeketol-acid reductoisomerase4e-104378
NC_010674:311378:3221283221283231441017Clostridium botulinum B str. Eklund 17B, complete genomeketol-acid reductoisomerase5e-104377
NC_014150:2501299:252190325219032522898996Brachyspira murdochii DSM 12563 chromosome, complete genomeketol-acid reductoisomerase8e-104377
NC_018643:937486:9440479440479450691023Alpha proteobacterium HIMB5 chromosome, complete genomeketol-acid reductoisomerase2e-103375
NC_018507:339000:3479833479833489991017Candidatus Portiera aleyrodidarum BT-B chromosome, complete genomeketol-acid reductoisomerase2e-103375
NC_018677:329829:3414003414003424161017Candidatus Portiera aleyrodidarum BT-B-HRs chromosome, completeKetol-acid reductoisomerase2e-103375
NC_019814:678404:6972846972846983001017Candidatus Kinetoplastibacterium blastocrithidii (ex Strigomonasketol-acid reductoisomerase1e-102373
NC_015318:706374:7085747085747095961023Hippea maritima DSM 10411 chromosome, complete genomeKetol-acid reductoisomerase1e-102373
NC_015732:3017387:3031869303186930328701002Spirochaeta caldaria DSM 7334 chromosome, complete genomeKetol-acid reductoisomerase3e-102372
NC_009012:2953638:298681029868102987805996Clostridium thermocellum ATCC 27405, complete genomeketol-acid reductoisomerase3e-102372
NC_013791:579842:5864885864885875161029Bacillus pseudofirmus OF4 chromosome, complete genomeketol-acid reductoisomerase3e-102371
NC_016887:4343830:4363973436397343649861014Nocardia cyriacigeorgica GUH-2, complete genomeKetol-acid reductoisomerase (Acetohydroxy-acid isomeroreductase) (Alpha-keto-beta-hydroxylacil reductoisomerase)9e-102370
NC_013520:1638000:1654683165468316556901008Veillonella parvula DSM 2008, complete genomeketol-acid reductoisomerase1e-101370
NC_013124:1606879:1623365162336516243871023Acidimicrobium ferrooxidans DSM 10331, complete genomeketol-acid reductoisomerase2e-101369
NC_016111:4645145:465215046521504653148999Streptomyces cattleya NRRL 8057, complete genomeacetohydroxy-acid isomeroreductase4e-101368
NC_007205:1:51651615291014Candidatus Pelagibacter ubique HTCC1062, complete genomeketol-acid reductoisomerase5e-101367
NC_016148:141011:1503121503121513221011Thermovirga lienii DSM 17291 chromosome, complete genomeketol-acid reductoisomerase2e-100365
NC_020209:3797830:3813085381308538141011017Pseudomonas poae RE*1-1-14, complete genomeketol-acid reductoisomerase7e-100363
NC_018681:7191008:7212318721231872133311014Nocardia brasiliensis ATCC 700358 chromosome, complete genomeketol-acid reductoisomerase2e-99362
NC_012438:50845:722637226373261999Sulfurihydrogenibium azorense Az-Fu1 chromosome, complete genomeketol-acid reductoisomerase4e-99361
NC_011999:936500:941110941110942102993Macrococcus caseolyticus JCSC5402, complete genomealpha-keto-beta-hydroxylacil reductoisomerase1e-98360
NC_009633:3457185:3467665346766534686661002Alkaliphilus metalliredigens QYMF chromosome, complete genomeketol-acid reductoisomerase7e-94343
NC_000909:1517461:1521358152135815224041047Methanocaldococcus jannaschii DSM 2661, complete genomeketol-acid reductoisomerase2e-92339
NC_014632:1248611:125483912548391255831993Ilyobacter polytropus DSM 2926 chromosome, complete genomeketol-acid reductoisomerase5e-92337
NC_000917:1778173:178341217834121784410999Archaeoglobus fulgidus DSM 4304, complete genomeketol-acid reductoisomerase1e-90333
NC_015474:1216971:123173712317371232726990Pyrococcus sp. NA2 chromosome, complete genomeketol-acid reductoisomerase8e-89327
NC_002754:491695:5062535062535072601008Sulfolobus solfataricus P2, complete genomeketol-acid reductoisomerase2e-85316
NC_003413:893960:900021900021901019999Pyrococcus furiosus DSM 3638, complete genomeketol-acid reductoisomerase8e-85313
NC_012623:1119192:1133924113392411349341011Sulfolobus islandicus Y.N.15.51 chromosome, complete genomeketol-acid reductoisomerase6e-84311
NC_017276:1335474:1337534133753413385411008Sulfolobus islandicus REY15A chromosome, complete genomeketol-acid reductoisomerase6e-84311
NC_012622:1421098:1423158142315814241651008Sulfolobus islandicus Y.G.57.14 chromosome, complete genomeketol-acid reductoisomerase6e-84311
NC_012588:1443753:1445813144581314468201008Sulfolobus islandicus M.14.25 chromosome, complete genomeketol-acid reductoisomerase5e-84311
NC_012632:1568170:1570230157023015712371008Sulfolobus islandicus M.16.27 chromosome, complete genomeketol-acid reductoisomerase5e-84311
NC_012726:1431745:1433805143380514348121008Sulfolobus islandicus M.16.4 chromosome, complete genomeketol-acid reductoisomerase5e-84311
NC_013769:1528196:1530256153025615312631008Sulfolobus islandicus L.D.8.5 chromosome, complete genomeketol-acid reductoisomerase5e-84311
NC_012589:1531725:1533785153378515347921008Sulfolobus islandicus L.S.2.15, complete genomeketol-acid reductoisomerase6e-84310
NC_000868:1299027:130441213044121305410999Pyrococcus abyssi GE5, complete genomeketol-acid reductoisomerase1e-83310
NC_017275:1486526:1488586148858614895931008Sulfolobus islandicus HVE10/4 chromosome, complete genomeketol-acid reductoisomerase4e-83308
NC_016645:1059482:109911310991131100099987Pyrobaculum sp. 1860 chromosome, complete genomeketol-acid reductoisomerase7e-82304
NC_010525:1360871:136876313687631369746984Thermoproteus neutrophilus V24Sta, complete genomeketol-acid reductoisomerase3e-80298
NC_009376:1551630:155163015516301552616987Pyrobaculum arsenaticum DSM 13514 chromosome, complete genomeketol-acid reductoisomerase2e-79296
NC_016885:324793:344572344572345564993Pyrobaculum oguniense TE7 chromosome, complete genomeketol-acid reductoisomerase2e-78292
NC_009073:1614320:163112916311291632115987Pyrobaculum calidifontis JCM 11548, complete genomeketol-acid reductoisomerase4e-78291
NC_015435:310406:3124573124573134641008Metallosphaera cuprina Ar-4 chromosome, complete genomeketol-acid reductoisomerase1e-76286
NC_003106:1435295:144986614498661450864999Sulfolobus tokodaii str. 7, complete genomeketol-acid reductoisomerase5e-76285
NC_015518:1052701:105471910547191055717999Acidianus hospitalis W1 chromosome, complete genomeketol-acid reductoisomerase1e-75283
NC_007181:1295956:133197913319791332977999Sulfolobus acidocaldarius DSM 639, complete genomeketol-acid reductoisomerase5e-74278
NC_009440:1857589:1873671187367118746781008Metallosphaera sedula DSM 5348 chromosome, complete genomeketol-acid reductoisomerase6e-74278
NC_014537:663216:670328670328671311984Vulcanisaeta distributa DSM 14429 chromosome, complete genomeketol-acid reductoisomerase3e-72272
NC_012026:1135041:115773611577361158707972Anaplasma marginale str. Florida, complete genomeketol-acid reductoisomerase (ilvC)2e-54212
NC_013532:85371:891878918790158972Anaplasma centrale str. Israel, complete genomeketol-acid reductoisomerase3e-54212
NC_004842:1132441:115352811535281154499972Anaplasma marginale str. St. Maries, complete genomeketol-acid reductoisomerase6e-54211
NC_014364:815346:8593208593208603331014Spirochaeta smaragdinae DSM 11293 chromosome, complete genomeketol-acid reductoisomerase6e-54211
NC_015160:3448570:3458083345808334591291047Odoribacter splanchnicus DSM 20712 chromosome, complete genomeketol-acid reductoisomerase3e-40166
NC_012622:650361:662867662867663865999Sulfolobus islandicus Y.G.57.14 chromosome, complete genomeketol-acid reductoisomerase3e-38159
NC_012623:2205891:221694622169462217944999Sulfolobus islandicus Y.N.15.51 chromosome, complete genomeketol-acid reductoisomerase3e-38159
NC_012589:564343:574324574324575322999Sulfolobus islandicus L.S.2.15, complete genomeketol-acid reductoisomerase3e-38159
NC_009749:879174:8902238902238912661044Francisella tularensis subsp. holarctica FTA, complete genomeketol-acid reductoisomerase3e-38159
NC_007880:877317:8884628884628895051044Francisella tularensis subsp. holarctica, complete genomeKetol-acid reductoisomerase3e-38159
NC_008369:880975:8921198921198931621044Francisella tularensis subsp. holarctica OSU18, complete genomeketol-acid reductoisomerase3e-38159
NC_015144:47783:7619776197772431047Weeksella virosa DSM 16922 chromosome, complete genomeketol-acid reductoisomerase2e-38159
NC_009257:1023696:1027244102724410282871044Francisella tularensis subsp. tularensis WY96-3418 chromosome,ketol-acid reductoisomerase4e-38158
NC_008601:1094041:1097585109758510986281044Francisella tularensis subsp. novicida U112, complete genomeketol-acid reductoisomerase4e-38158
NC_002754:1749834:176064817606481761646999Sulfolobus solfataricus P2, complete genomeKetol-acid reductoisomerase (ilvC-3)6e-38158
NC_014933:3942156:3955037395503739560801044Bacteroides helcogenes P 36-108 chromosome, complete genomeketol-acid reductoisomerase1e-37157
NC_017275:568722:578344578344579339996Sulfolobus islandicus HVE10/4 chromosome, complete genomeketol-acid reductoisomerase1e-37157
NC_011565:469962:4735474735474745931047Candidatus Azobacteroides pseudotrichonymphae genomovar. CFP2,ketol-acid reductoisomerase9e-38157
NC_010336:1670000:1681623168162316826661044Francisella philomiragia subsp. philomiragia ATCC 25017, completeKetol-acid reductoisomerase2e-37156
NC_017025:1278374:1301992130199213030351044Flavobacterium indicum GPTSA100-9, complete genomeketol-acid reductoisomerase8e-37154
NC_014759:1957979:1966415196641519674611047Marivirga tractuosa DSM 4126 chromosome, complete genomeketol-acid reductoisomerase1e-36153
NC_011184:2840713:2845980284598028474641485Vibrio fischeri MJ11 chromosome I, complete sequenceketol-acid reductoisomerase6e-36151
NC_004347:4541742:4541742454174245432201479Shewanella oneidensis MR-1, complete genomeketol-acid reductoisomerase7e-35148
NC_014972:3771642:3771642377164237731171476Desulfobulbus propionicus DSM 2032 chromosome, complete genomeketol-acid reductoisomerase3e-34145
NC_008571:2229369:2238749223874922402241476Gramella forsetii KT0803, complete genomeketol-acid reductoisomerase1e-33144
NC_015914:2769042:2778964277896427800071044Cyclobacterium marinum DSM 745 chromosome, complete genomeketol-acid reductoisomerase1e-33143
NC_012918:1697860:1735563173556317370351473Geobacter sp. M21 chromosome, complete genomeketol-acid reductoisomerase4e-33142
NC_010506:537833:5413885413885428691482Shewanella woodyi ATCC 51908, complete genomeketol-acid reductoisomerase1e-32140
NC_009831:5107924:5123306512330651247871482Shewanella sediminis HAW-EB3, complete genomeKetol-acid reductoisomerase6e-32138
NC_010634:166900:1713381713381728161479Yersinia pseudotuberculosis PB1/+, complete genomeketol-acid reductoisomerase3e-30132
NC_006155:172313:1767461767461782241479Yersinia pseudotuberculosis IP 32953, complete genomeketol-acid reductoisomerase3e-30132
NC_006138:799174:803305803305804018714Desulfotalea psychrophila LSv54, complete genomeketol-acid reductoisomerase4e-30132
NC_005061:647567:6555436555436570211479Candidatus Blochmannia floridanus, complete genomeketol-acid reductoisomerase9e-30130
NC_014909:662500:6700966700966715921497Candidatus Blochmannia vafer str. BVAF chromosome, complete genomeketol-acid reductoisomerase2e-29129
NC_006138:799174:803981803981804778798Desulfotalea psychrophila LSv54, complete genomeketol-acid reductoisomerase2e-0653.5